1.International Indicators for Rational Drug Use in Outpatient Prescriptions of 21 Medical Institutions in Shanghai Songjiang District
China Pharmacy 2005;0(19):-
OBJECTIVE: To investigate the situation of rational use of drugs in the outpatient prescriptions in 21 medical institutions in Shanghai Songjiang district.METHODS: The international indicators for rational drug use were employed to evaluate outpatient prescriptions in 21 medical institutions in Shanghai Songjiang district.RESULTS: As for the international indicators for rational drug use in the outpatient prescriptions,an average of 2.5 kinds of drugs were prescribed per prescription in 21 hospitals,42% prescriptions prescribed antibacterials,20% prescribed injections,and 43.97% prescribed basic drugs of zero difference rate.CONCLUSIONS: The drug use in outpatients of 21 medical institutions was rational on the whole;however,the proportions of prescribing antibacterials and injections in some hospitals were still on the high side. Thus the rational drug use level remains to be improved further.
4.One New Method of Nucleic Acid Amplification-Loop-mediated Isothermal Amplification of DNA
Xueen FANG ; Jian LI ; Qin CHEN
Virologica Sinica 2008;23(3):167-172
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.
5.The clinical analysis of ultrasound biomicroscope scanning photography in anterior segment structure changes in blunt eye injury
Jian ZENG ; Jing CHEN ; Lingzhu FANG
Chinese Journal of Ultrasonography 1993;0(02):-
Objective To clinical analyze the changes of ocular anterior segment structure using ultrasound biomicroscope(UBM) and their clinical values in blunt eye injury.Methods Sixty-nine eyes with no-opening wound in blunt eye injury were examined by UBM(Paradigm Model P40).Scanning photography of central and 3,6,9,12 clock hour were recorded and printed,other clock hours were scanned if needed.These records were studied with clinical findings.Results UNM findings showed that 27 eyes had hyphema,22 eyes had anterior chamber angle recession,16 eyes had iridodialysis,19 eyes had local luxation of lens,5 eyes had vitreous henia in anterior chamber,19 eyes had cyclodialysis,23 eyes anterior choroidal detachment and 9 eyes had anterior vitreous haemorrhage.Of the 69 eyes,62 eyes had combined structure changes showed above,which account(89.9)%.Conclusions UBM is a practical tool for diagnosis of anterior segment structure changes in blunt eye injury,especially when there are corea opacity,hyphema,cyclodialysis,ocular hypotension and others.It can provide exact informations for the diagnosis and treatment,which can not be showed by routine ocular examination and B-scanning ultrasonography.
6.Application of Antibacterials for the Inpatients with Chronic Obstructive Pulmonary Disease in Our Hospital
Weijun FANG ; Jian CHEN ; Junyan LI
China Pharmacy 2005;0(20):-
OBJECTIVE:To investigate the application of antibacterials in patients with chronic obstructive pulmonary disease(COPD)in our hospital.METHODS:A retrospective analysis was performed on 77 COPD inpatients with respect to the varieties of antibiotics,DDDs,drug costs,routes of administration,medication duration,combined use of antibiotics,therapeutic outcome,bacterial culture and susceptibility test,adverse drug reactions(ADR)and irrational drug use etc.RESULTS:100% of the patients received antibacterials.Topping the first five places on the DDDs list were levofloxacin,cefuroxime,amoxicillin/clavulanate potassium,ceftazidime and azithromycin.The combined use of two kinds of antibiotics accounted for 77.65% versus 2.35% of three kinds concomitantly.CONCLUSION:The use of antibacterials in COPD patients in our hospital is basically rational;however,the indications of antibacterials for COPD patients should be followed strictly toachieve rational and standard drug use.
7.Progress in glucaric acid.
Yuying QIU ; Fang FANG ; Guocheng DU ; Jian CHEN
Chinese Journal of Biotechnology 2015;31(4):481-490
Glucaric acid (GA) is derived from glucose and commonly used in chemical industry. It is also considered as one of the "Top value-added chemicals from biomass" as carbohydrate monomers to produce various synthetic polymers and bioenergy. The demand for GA in food manufacture is increasing. GA has also attracted public attentions due to its therapeutic uses such as regulating hormones, increasing the immune function and reducing the risks of cancers. Currently GA is produced by chemical oxidation. Research on production of GA via microbial synthesis is still at preliminary stage. We reviewed the advances of glucaric acid applications, preparation and quantification methods. The prospects on production of GA by microbial fermentation were also discussed.
Biomass
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Chemical Industry
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Fermentation
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Glucaric Acid
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chemistry
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Glucose
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Polymers
8.Substrate specificities of bile salt hydrolase 1 and its mutants from Lactobacillus salivarius.
Jie BI ; Fang FANG ; Yuying QIU ; Qingli YANG ; Jian CHEN
Chinese Journal of Biotechnology 2014;30(3):445-454
In order to analyze the correlation between critical residues in the catalytic centre of BSH and the enzyme substrate specificity, seven mutants of Lactobacillus salivarius bile salt hydrolase (BSH1) were constructed by using the Escherichia coli pET-20b(+) gene expression system, rational design and site-directed mutagenesis. These BSH1 mutants exhibited different hydrolytic activities against various conjugated bile salts through substrate specificities comparison. Among the residues being tested, Cys2 and Thr264 were deduced as key sites for BSH1 to catalyze taurocholic acid and glycocholic acid, respectively. Moreover, Cys2 and Thr264 were important for keeping the catalytic activity of BSH1. The high conservative Cys2 was not the only active site, other mutant amino acid sites were possibly involved in substrate binding. These mutant residues might influence the space and shape of the substrate-binding pockets or the channel size for substrate passing through and entering active site of BSH1, thus, the hydrolytic activity of BSH1 was changed to different conjugated bile salt.
Amidohydrolases
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genetics
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metabolism
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Bile Acids and Salts
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metabolism
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Escherichia coli
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metabolism
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Gene Expression
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Lactobacillus
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enzymology
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genetics
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Substrate Specificity
9.The mechanism of RCE-4, an active ingredient of Reineckia carnea , in combination with celecoxib on the anti-proliferation of cervical cancer Ca Ski cells
Hong-heng ZHEN ; Fang-fang YOU ; Fan CHENG ; Kun ZOU ; Chong-xu CHEN ; Jian-feng CHEN
Acta Pharmaceutica Sinica 2021;56(7):1911-1920
This research explored the synergistic effects and the potential mechanisms of RCE-4 and various nonsteroidal anti-inflammatory drugs (NSAIDs) on the proliferation of cervical cancer Ca Ski cells. The MTT assay and CalcuSyn V2.0 software were used to detect cell proliferation and calculate the combination index (CI); the expression levels of various proteins were analyzed using Western blot assay; mitochondrial membrane potential (MMP) was assessed using JC-1 staining; acridine orange/ethidium bromide (AO/EB) double-fluorescence staining was used to detect the apoptosis of Ca Ski cells; a co-immunoprecipitation (Co-IP) assay was used to analyze the relative content of Bcl-2-Beclin 1 complex in Ca Ski cells. The results demonstrate that the combination of RCE-4 and NSAIDs increases the inhibition of Ca Ski cells compared to the single-RCE-4 group, and celecoxib provided the best synergistic effect among the four NSAIDs tested, with a CI of 0.32. The combination of RCE-4 and celecoxib significantly down-regulated the expression of cyclooxygenase-2 (COX-2) and nuclear transcription factor-