1.Effect of exogenous PGC -1α on VEGF expression in human retinal vascular endothelial cells
Jian, JIANG ; Xiao-Bo, XIA ; Lu, ZHANG
International Eye Science 2015;(2):198-200
AlM: To investigate the effect of exogenous peroxisome-proliferator-activated receptor-γcoactivator-1α ( PGC-1α) on vascular endothelial growth factor ( VEGF) expression in human retinal vascular endothelial cells ( HRVEC) .METHODS:Recombinant PGC-1α protein was added to HRVEC, and no recombinant PGC-1α protein was added to HRVEC as control group. After 24h of incubation, two groups of cells were then placed into a normoxic ( 20%O2 ) or hypoxic ( 1% O2 ) environment for another 16h. The expression of VEGF mRNA and protein were detected by real - time PCR, ELlSA and immunofluorescence cytochemistry.RESULTS: VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC - 1αprotein both under normoxia and hypoxia conditions as compared with control groups (P<0. 01).CONCLUSlON: PGC-1α can upregulate the expression of VEGF in HRVEC under normoxia and hypoxia conditions.
5.A Comparative Study on Sensitivity of HE,IHC and RT-PCR in Detection of Breast Cancer Metastases in Axillary Lymph Nodes
Bo CHEN ; Yunfei LU ; Jian ZENG ; Sheng HE
Chinese Journal of Bases and Clinics in General Surgery 2003;0(03):-
Objective To compare the sensitivity of HE,immunohistochemistry (IHC) and RT-PCR in detection of breast cancer metastases in axillary lymph nodes.Methods Twenty female patients with newly diagnosed and clinically node-negative breast cancers underwent modified radical mastectomy, including a complete axillary lymph node dissection. The ages of the patients ranged from 31 years to 65 years, and the diagnosis of breast cancer was approved by pathological finding. Two hundred and thirty-nine axillary lymph nodes were found in these 20 patients. Metastases in axillary lymph nodes were explored by HE, cytokeratin 19 IHC and RT-PCR for cytokeratin 19 respectively. Results Seven(2.9%) lymph nodes were found to have metastatic cancers by HE in 3 patients,all nodes were found in level Ⅰ. Metastatic cancers were found in 13 (5.4%) nodes by IHC in 7 patients,11 nodes in level Ⅰ and 2 nodes in level Ⅱ; and 52(21.8%) nodes were found to contain tumor cells by RT-PCR in 14 patients,30 nodes in level Ⅰ and 22 nodes in level Ⅱ. All of 7 histologically(HE) positive nodes were found to contain tumor cells by IHC and RT-PCR. Among 232 histologically(HE) negative nodes,6(2.6%) nodes were found to contain tumor cells by IHC,and 45(19.4%) nodes were found to contain tumor cells by RT-PCR, all 6 IHC positive nodes showed the expected 460-base pair products on gel electrophoresis ( P
6.Effect of Diabetic Status on the Preventive Efficiency of Methylcobal on Peripheral Neuropathy in Rats
Jian-bo LI ; Jia-wei CHEN ; Xiao-lu LI
Chinese Journal of Rehabilitation Theory and Practice 2006;12(10):861-862
ObjectiveTo investigate the effect of varied hyperglycemia and durations on preventing peripheral neuropathy (PN) with methylcobal in diabetic rats.Methods80 Sprague Dawley rats were selected and randomly chosen 16 as normal control (NC) group, others 64 animals as diabetic model induced by alloxan. The model rats were evenly divided into relatively good control (GC) group and relatively poor control (PC) group based on levels of hyperglycemia regulated with exogenous insulin. 16 rats of each GC/PC group were intramuscularly injected with methylcobal (500 μg/kg). The sensory nerve conduction velocity (SNCV), motor nerve conduction velocity (MNCV) and evoked potential amplitute (EPA) of sciatic nerve of rats were detected by evoked electromyogram respectively at 2nd, 8th and 12th week after onset of diabetes. Fructosamine in serum was also detected.ResultsThe rats injected with methylcobal had a significantly delayed reduction of SNCV, MNCV and EP in GC group (fructosamine,1.0 mmol/L) than that in PC group (fructosamine, 1.2 mmol/L) ( P<0.05~0.01). Up to 8th week after onset of diabetes, there was no obvious difference in physioelectroparameters between the GC group and NC group. At 12th week after onset of diabetes, physioelectroparameters in the PC group fell to the level of non-methylcobal injected group. No effect of methylcobal on blood glucose revealed in any groups ( P>0.05).ConclusionMethylcobal has a beneficial effect on delaying the onset of diabetic PN, and is of high efficacy with a good control of diabetic state, especially at early stage.
7.Correlative Study on Acute Ischemic Cerebrovascular Disease and Chlamydia Pneumoniae in Children
jian, CHANG ; dong, LIANG ; dian-bo, CAO ; ji-rong, LU ; yin-bo, CHEN
Journal of Applied Clinical Pediatrics 2003;0(10):-
0.05).Between subgroups divided by the clinical restoration time,the CP IgM seropositive rate of the tachy restoration group was significantly higher than that in the deferred group(P0.05).Among subgroups divided by the clinical manifestation,the subgroup of paralysis plus disorder of consciousness had significant higher CP IgM seropositive rate than other groups(Pa
8.A wireless wide area network picture archiving and communication system based on open source code and its clinical application
Jian MA ; Jian-Huan LU ; Yun-Bo ZHOU ; Xiao-Dan MA ;
Chinese Journal of Radiology 2000;0(11):-
Objective To build a set of inexpensive wireless wide area network PACS based on open source code.Methods To improve the open source code MyFreePACS on Internet.Results The basic function of PACS can be achieved in an economical way,using internet explorer to browse and deal with the DICOM Images,with the capability of such functions as displaying and managing of the pictures.It is possible to make rounds at patient bedside with wireless device and remote medical consultation with specialists through Internet.Conclusion It is possible to utilize the free open source code MyFreePACS on the internet to construct an economical PACS on Web in the medium and small-scale hospital.
9.Change of Interleukin-2 in Nasopharyngeal Secretion of Children with Acute Respiratory Syncytial Virus Bronchitis
jian, CHANG ; dong, LIANG ; ji-rong, LU ; yin-bo, CHEN ; hong-xia, ZHAO
Journal of Applied Clinical Pediatrics 1994;0(04):-
0.05).Conclusion There is no association between IL-2 levels in NS and RSV bronchitis.The IL-2 levels show a heterogenous behavior.
10.Preoperative renal artery infusion chemoembolization for intermediate-and advanced-stage renal carcinoma
Jian XU ; Jianmin CAO ; Guangming LU ; Weidong KONG ; Dazhi GAO ; Bo CHEN
Journal of Medical Postgraduates 2003;0(08):-
Objective:It is difficult to separate intermediate-and advanced-stage renal carcinoma,and in some cases even harder to totally remove it.This study aimed to assess the effect of the renal artery infusion chemoembolization on intermediate-and advanced-stage renal carcinoma before surgery.Methods: Twenty-seven patients with intermediate-or advanced-stage renal carcinoma accepted the Sandwich mode of selective bronchial renal artery embolization 3-7 days before surgery.Then we analyzed both the methods of embolization and surgery after intervention therapy.Results: All the patients were successfully treated by transarterial embolization.During the operation,renal artery pulsatility significantly decreased or even disappeared,with obvious swelling of the surrounding renal tissues but very little bleeding.Conclusion: Preoperative chemoembolization for intermediate-and advanced-stage renal carcinoma can improve surgical conditions,shorten the operation time,decrease bleeding and elevate the success rate.