Objective To observe the effectiveness of comprehensive rehabilitation on the recovery of motor function of patients with severe cerebral injury in recovery phase.Methods 72 cases with severe cerebral injury in recovery phase were randomly divided into rehabilitation group (n=38) and control group (n=34). Patients in control group were treated with routine method, and those in rehabilitation group with comprehensive rehabilitation treatment in addition.Results Motor function, activities of daily living (ADL) and complication of patients were evaluated at the first day and two months later after be in hospital. The effect of comprehensive rehabilitation treatment were better than that of control group(P<0.05,P<0.01). Conclusion Comprehensive rehabilitation is important to the patients with severe cerebral injury of recovering, not only in recovery of motor function but also in reducing the complication.

ObjectiveTo compare the therapeutic effects of percutaneous cervical discectomy (PCD group),percutaneous cervical disc nucleoplasty(PCN) and the association of them (PCDN) for the treatment of cervical intervertebral disk displacement and instability of cervical vertebral column.Methods From February 2003 to April 2011,171 consecutive patients with cervical disc herniation have presented at the authors' hospital and were retrospectively studied.The average age of patients was 47.8 years(ranged,21-74).Ninety-seven cases were treated with PCD,50 cases with PCN,and the other 24 cases with PCDN.Clinical result and the stability of cervical vertebral column after operation were evaluated and compared among the 3 groups.ResultsAll cases had been followed up for a median of 4.1 years.There was significant difference in the pre- and post-operation the Japanese Orthopaedic Association(JOA) scoring system on within 3groups (PCD:t=21.85,P＜0.05; PCN:t=14.50,P＜0.05; PCDN:t=8.56,P＜0.05).All cases had been successfully operated.There was no significant difference between groups among the 3 groups in terms of the clinical outcomes(The recovery rate of JOA standard evaluation,F=2.19,P=0.12).According to Odom criteria,the excellent and good rate are as follows:81.35％ in PCD,82.44％ in PCN,83.19％ in PCDN,respectively.There was no significant difference between groups among the 3 groups in terms of the clinical success rate (P＞0.05).There was no instability of cervical vertebral column cases in 3 groups after operation(P＞0.05),and no significant difference was found in terms of cervical vertebral column stability in pre- and post-operation in each group.ConclusionAll the three operations including PCN,PCD and PCDN are safe,minimally invasive spine surgery for the treatment of cervical intervertebral disk displacement; they achieve good clinical outcomes and there are no difference on the stability of cervical vertebral column between preoperation and postoperation.

Accidents, Occupational ; Adolescent ; Adult ; Arsenic Poisoning ; Child ; Female ; Humans ; Male ; Middle Aged ; Water Pollution, Chemical

Objective To explore high density lipoprotein(HDL)/low density lipoprotein(LDL)and total type Ⅰ collagen amino terminal extender peptide(t-PINP)/C-terminal peptide of type Ⅰ collagen β special sequence(β-CTX)and risk of os-teoporosis vertebral fractures(OPVFs)in elderly women.Methods The clinical data of 446 female OPVFs patients aged above 60 years old from January 2019 to December 2020 were retrospectively analyzed.According to whether or not fracture,patients were divided into non-fracture group(186 patients)and fracture group(260 patients).Univariate analysis was performed to analysis age,body mass index(BMI),N-terminal mioldle molecular fragment of osteocalcin,N-MID OC),t-PINP,β-CTX,25-hydroxyvitamin D[25-(OH)VitD],blood sugar(Glu),total cholesterol(TC),high-density lipoprotein(HDL),low-density lipoprotein(LDL),Ca,P,Mg,urea(UREA),creatinine(Cr)and Cystatin C(CysC),and correlation between OPVFs and the above indexes and lipid,bone metabolism indexes between two groups;Logistic regression was performed to analyze risk factors and stratification relationship between vertebral fracture and HDL/LDL,t-PINP/β-CTX.Logistic regression was used to ana-lyze risk factors and stratification relationship between OPVFs and HDL/LDL,t-PINP/β-CTX.Results There were no signif-icant difference in age and BMI between non-fracture group and fracture group(P＞0.05).Compared with non-fracture group,contents of HDL,t-PINP/β-CTX and HDL/LDL in fracture group were decreased,and contents of β-CTX were increased(P＜0.05).OPVFs was positively correlated with β-CTX(r=0.110,P＜0.05),and negatively correlated with HDL,HDL/LDL and t-PINP/β-CTX(r=-0.157,-0.175,-0.181,P＜0.05).HDL and HDL/LDL were negatively correlated with β-CTX(r=-0.22,-0.12,P＜0.05)and t-PINP(r=-0.13,-0.10,P＜0.05).25-(OH)VitD was positively correlated with TC and HDL(r=0.11,0.18,P＜0.05).HDL/LDL was positively correlated with t-PINP/β-CTX(r=0.11,P=0.02).t-PINP/β-CTX[OR=0.998,95％CI(0.997,1.000),P＜0.05],HDL/LDL[OR=0.228,95％CI(0.104,0.499),P＜0.01]were risk factors for vertebral fracture.The lower levels between two tristratified indicators,the higher the vertebral fracture rate.The risk of fracture was 2.5 and 2 times higher in the lowest stratum than in the highest stratum,with an adjusted OR was[2.112,95％CI(1.310,3.404)]and[2.331,95％CI(1.453,3.739)],respectively.Conclusion Serum low HDL/LDL and t-PINP/β-CTX are independent risk factors for OPVF in elderly women,and have good predictive value for OPVF risk.

To explore the method of explants directly induced bud and establish the tissue culture system of mutiple shoot by means of direct organogenesis, core bud and daughter bulbs (the top of bud stem expanded to form daughter bulb) of T. edulis were used as explants and treated with thidiazuron (TDZ) and 1-naphthlcetic acid (NAA). The results showed that the optimal medium for bud inducted form core bud and daughter bulb were MS + TDZ 2.0 mg x L(-1) + NAA 4.0 mg x L(-1) and MS +TDZ 2.0 mg x L(-1) + NAA 2.0 mg x L(-1) respectively, both of them had a bud induction rate of 72.92%, 79.22%. The optimal medium for cluster buds multiplication was MS + TDZ 0.2 mg x L(-1) + NAA 0.2 mg x L(-1), and proliferation coefficient was 2.23. After proliferation, cluster buds rooting occurred on MS medium with IBA 1.0 mg x L(-1) and the rooting rate was 52.6%, three to five seedlings in each plant. Using core bud and daughter bulb of T. edulis, the optimum medium for adventitious bud directly inducted from daughter bulb, core bud and cluster bud multiplication were screened out and the tissue culture system of multiple shoot by means of direct organogenesis was established.
Naphthaleneacetic Acids ; pharmacology ; Phenylurea Compounds ; pharmacology ; Plant Growth Regulators ; pharmacology ; Plant Shoots ; drug effects ; growth & development ; Plant Stems ; drug effects ; growth & development ; Seedlings ; drug effects ; growth & development ; Thiadiazoles ; pharmacology ; Tissue Culture Techniques ; Tulipa ; drug effects ; growth & development

OBJECTIVETo investigate the changes of hair dermal papilla and its regulatory role in the growth cycle of the hair follicles.

METHODSSingle hair follicles were isolated from surgical specimens of human scalp and cultured in Williams E medium. The growth of the hair follicle was measured and the morphology and structure of the dermal papilla in the different growth cycles were observed continuously.

RESULTSThe hair follicle could grow in the medium for 12 days at the average growth rate of 0.2-0.3 mm/day. The flat and round dermal papilla lay at the bottom of the hair bulb in the telogen and anagen stages. In the hair follicle with accelerated growth, the dermal papilla became elongated, loosened, and closely adhered to the hair matrix. In the catagen stage the dermal papilla shrunk, and became separated from the hair matrix. A new hair bulb was regenerated when the hair follicle was transected at a low level. The hair follicle stopped growing after transection at a higher position.

CONCLUSIONThe hair dermal papilla is the essential for hair follicle growth, and plays an important role in regulating the hair growth cycle.

Dermis ; cytology ; growth & development ; Hair ; growth & development ; Hair Follicle ; cytology ; growth & development ; Humans ; Tissue Culture Techniques

OBJECTIVETo study the methylation of O-6-methylguanine-DNA methyltransferase (MGMT) and p16 gene in the sputum cells of radon-exposed population. To provide the experimental base for finding the molecular biomarker of the high risk population of the radon-induced lung cancer.

METHODS91 radon-exposed workers were divided into 4 groups, high dosage group (> 120 WLM), middle dosage group (between 60 and 120 WLM), low dosage group (between 30 and 60 WLB) and lower dosage group (between 2 and 30 WLM) according to the accumulated exposure dosage of the radon daughters. The abnormal methylation of p16 and MGMT gene in the sputum cells of the population in the four groups was detected with the methylation specific PCR (MSP).

RESULTSThere was significantly upward trend for the p16 gene methylation rate (0.00%-20.00%), the MGMT gene methylation rate (0.00%-28.00%) and the total methylation rate (0.00%-40.00%) with the increase of the accumulated exposure dosage of the radon daughters (P < 0.01).

CONCLUSIONThe methylation of p16 and MGMT gene is related to the accumulate exposure dosage of the radon daughters.

Carcinogens, Environmental ; adverse effects ; Cyclin-Dependent Kinase Inhibitor p16 ; genetics ; metabolism ; DNA Methylation ; Dose-Response Relationship, Radiation ; Humans ; Male ; O(6)-Methylguanine-DNA Methyltransferase ; genetics ; metabolism ; Occupational Exposure ; Radon ; adverse effects ; Radon Daughters ; adverse effects ; Sputum ; metabolism

Adolescent ; Adult ; Aged ; Alanine Transaminase ; blood ; Female ; Hepatitis B virus ; growth & development ; Hepatitis B, Chronic ; blood ; virology ; Humans ; Male ; Middle Aged ; Viral Load

Objective To evaluate the interactive effects of fine particulate matter and temperature on non-accidental mortality of residents in Pudong, Shanghai. Methods Daily mortality, air pollutants and meteorological data from Jan 1^st.2016 to Dec 31^st.2017 were collected.Generalized additive Poisson regression models was used to estimate the effects of PM_2.5 pollution on daily mortality, bivariate response surface models and temperature stratified models were applied to examine the interaction of temperature with PM_2.5 on mortality. Results A total of 43 345 non-accidental deaths were included, daily mean PM_2.5 concentration was 39.1 μg/m³, daily mean temperature was 17.7 ℃.A 10 μg/m³ increase in the daily PM_2.5 at lag 1 day was associated with a 0.56%(95%CI:0.11%-1.01%), 0.49%(95%CI:-0.19%-1.18%) and 0.22%(95%CI:-1.14%-1.60%) increase in non-accidental, cardiovascular and respiratory mortality, respectively.Higher risks were identified for males and the older (≥65 years).The effect estimates per 10 μg/m³ increase in PM_2.5 in medium temperature level were 0.59%(95%CI:0.04%-1.14%)for non-accidental, 0.51%(95%CI:-0.32%-1.35%)for cardiovascular and 0.51%(95%CI:-0.32%-1.35%) for respiratory mortalities.The PM_2.5 effects were approximately 2-4 times higher in higher temperature level for non-accidental and cardiovascular mortalities compared with other temperature levels; for respiratory mortality, the PM_2.5 effects was approximately 2-fold higher in lower temperature levels than the medium, although the interactions between temperature and PM_2.5 were statistically insignificant. Conclusions Temperature may modify the effects of PM_2.5 on mortality in Pudong, Shanghai and the interactive pattern may be different across disease-specific mortalities.

Objective: To facilitate the functional analysis of chromosomal genes and their products, the recombineering technique to epitope tagging of chromosomal genes of Y. pestis was adapted. Methods: The epitope tag was generated by primer annealing and then fused with resistance gene by fusion PCR. The epitope-resistance cassette was inserted into pBluecript, resulted in the template plasmid, pBS-MH. The tagging cassette for rpoS was obtained by PCR amplification from pBS-MH with primers containing homology specific to the target gene. PCR products were transformed into recombination competent cells and recombinants were selected. PCR and DNA sequencing were used to confirm the correct tagging event. The expression of the tagged protein was detected with Western blot by using monoclonal antibody to the epitope. Results: The template plasmid containing fusion of epitope and resistance gene was successfully constructed. The sigma factor gene, rpoS, was tagged with a myc-his tag at the COOH terminus. Expression of the tagged rpoS was successfully detected indirectly by the antibody against His tag. Conclusion: The chromosomal gene tagging by recombineering technique represents a powerful tool in the functional study of bacterial genes and their products.