1.Construction of monitoring system on chemical contaminant in Chinese export plant food and it's application.
Guang-jiang TANG ; Yong-ning WU ; Jian-zhong SHEN
Chinese Journal of Preventive Medicine 2010;44(7):584-586
China
;
Food
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Food Contamination
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prevention & control
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Food Inspection
;
methods
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Plants
;
chemistry
2.Progresses in molecular biologic studies on coagulase negative staphylococcus infection.
Jian-hui DI ; Xu-zhuang SHEN ; Yong-hong YANG
Chinese Journal of Pediatrics 2004;42(1):26-29
Bacteremia
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etiology
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Catheterization
;
adverse effects
;
Child
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Coagulase
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metabolism
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Cross Infection
;
etiology
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Drug Resistance, Bacterial
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drug effects
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Endocarditis, Bacterial
;
etiology
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Humans
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Methicillin
;
pharmacology
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Quinolones
;
pharmacology
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Staphylococcal Infections
;
complications
;
drug therapy
;
microbiology
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Staphylococcus
;
classification
;
drug effects
;
pathogenicity
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Urinary Tract Infections
;
etiology
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Vancomycin
;
pharmacology
3.Practice and the reflection on the new model of public hospital reform at Beijing Children’s Hospital Group
Xin NI ; Yong LIU ; Jian TIAN ; Hui WANG ; Ying SHEN
Chinese Journal of Hospital Administration 2015;(9):654-657,658
In 2012,Beijing Children’s Hospital established the Beijing Children’s Hospital Group to explore the new model of public hospital reform.This study covered the target setting,management framework,network system,and sharing mechanism of the group,and discussed the merits and setbacks of such a practice,for insights of public hospital reform.
4.Design and Application of Distal Radius Anatomical Shape Bracket Splints.
Mao WU ; Yong LIU ; Jie-feng SHEN ; Yong MA ; Jian-wei WANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(5):555-558
OBJECTIVETo observe the clinical effect of anatomic shape bracket splints designed by 3D-MAX software.
METHODSHealthy adult volunteers' forearms and wrist data were measured and processed by 3D-MAX software. Then we designed and made anatomic shape bracket splints. Totally 60 distal radial fracture patients were equally assigned to the test group and the control group. After manual reduction, patients in the test group used anatomic shape bracket splints, while those in the control group used common splints. The palmar dip angle, ulnar fleet angle, and radius height of standard X-ray in normal and lateral positions were measured after treatment, at 1, 3, and 6 week after treatment, respectively. Meanwhile, the incidence of complications was recorded during the whole treatment process.
RESULTSCompared with the control group, the palmar dip angle and ulnar fleet angle were larger in the test group after one week of treatment (P < 0.05). The radius height of the treatment group was higher after one, three, and six weeks of treatment (P < 0.05). Compared with the control group, the incidence of complications was obviously less in the test group (P < 0.05).
CONCLUSIONSAnatomic shape bracket splints designed by 3D-MAX software fit local anatomic features and need no more shaping. Its easier use with lesser complications could maintain the stability of bone fracture better.
Adult ; Humans ; Imaging, Three-Dimensional ; Radius ; Radius Fractures ; therapy ; Splints ; Wrist Joint
6.Genetic Transformation of Candida glycerinogenes by REMI and Electroporation
Yong-Guang ZHANG ; Wei SHEN ; Zhi-Ming RAO ; Hui-Ying FANG ; Jian ZHUGE ;
Microbiology 1992;0(05):-
In order to isolate genes related with the osmoadaptation and glycerol metabolism of Candida glycerinogenes, a transformation system based on the dominant selectable marker Zeocin and restriction enzyme-mediated integration (REMI) was established. Effects of seven restriction enzymes on transformation efficiency of C.glycerinogenes were tested. Transformation conditions were optimized in the presence of Hind III. Under the optimal conditions of OD_ 600 ≈1.3, voltage of 1.5 kV, 2.0?10~9 competent cells/mL, 100 units of Hind III added, the transformation efficiency was up to 129 trnaformants/?g DNA. 58% of transformants were stable on nonselective medium. These results suggest that REMI technique would be beneficial to the genetic transformation of C.glycerinogenes.
7.The effect of endostatin mediated by adenovirus on the inflammation and cytokines of arthritis rats
De-Qian MENG ; Yu ZHANG ; Wei-Gan SHEN ; Yong-Ji XUE ; Li-Chun XU ; Jian GU ;
Chinese Journal of Rheumatology 2003;0(12):-
Objective To investigate the effect of recombinant adenovirus mediatied human endostatin (rAD-GFP-ES)on rats with collagen typeⅡinduced arthritis(CIA),and explore the mechanism of inflamma- tion and cytokines inhibition on rats CIA.Methods The rAD-GFP-ES was amplified and purified.The model of rat CIA was induced by intradermal injection of typeⅡcollagen combined with complete Freund's adjuvant(CFA). On the second day after the injection,the therapeutic administration of rAD-GFP-ES(1?10~(11)pfu?kg~(-1)?week~(-1)?4 weeks)were performed to the rats.The mean arthritis index(AI)was scored every week since then.The relative concentrations of ES,IL-I?,TNF-?in sera collected at the fourth week were evaluated by western blotting. Results①The titer of the purified rAD-GFP-ES and rAD-GFP was 6.6?10~(12)pfu/ml and 4.8?10~(12)pfu/ml,re- spectively(A_(260nm)/A_(280nm)>1.3).②The concentration of ES in sera of the group treated with rAD-GFP-ES was 2.4-lold higher compared to the normal group.③The mean arthritis index of the group treated with rAD-GFP- ES was much lower than that of the model group.The administration of rAD-GFP-ES could significantly de- creas the production of IL-1?and TNF-?in sera.Conclusions①The rAD-GFP-ES is efficiently expressed in vivo.②The rAD-GFP-ES has an inhibitory effect on the arthritis index of rat CIA.③IL-1?and TNF-?are involved in the pathogenesis of RA.The rAD-GFP-ES has an inhibitory effect on the expression of IL-1?and TNF-?in rat CIA.
8.Expressions of IL-1?,IL-6,SCF,TPO on bone marrow stromal cells in patients with multiple myelo-ma
Wen-Yi SHEN ; Hua LU ; Xiao-Ming FEI ; Peng LIU ; Jian-Yong LI ;
Cancer Research and Clinic 2006;0(11):-
Objective To study multiple cytokine mRNA expressions of bone marrow stromal cells in patients with multiple myeloma(MM),as well as to explore the role of these cytokines in the occurrence and development of MM.Methods Semi-quantitiative RT-PCR was used to detect the mRNA expressions of IL- 1?,IL-6,SCF,TPO.Results The mRNA expression levels of IL-1? and IL-6 were higher than that of nor- mal controls and other hematological malignancies(P
9.Short-term clinical outcome of single level degenerative lumbar diseases treated by minimally invasive surgery oblique lumbar interbody fusion
Junhong SHEN ; Jian WANG ; Chao LIU ; Changqing LI ; Chao ZHANG ; Yong PAN ; Yue ZHOU
Chinese Journal of Orthopaedics 2017;37(16):997-1005
Objective To investigate the short-term clinical outcome of one-level degenerative diseases for a single surgeon during his initial phase of performing a minimally invasive surgery oblique lumbar interbody fusion (OLIF) on the basis of perioperative parameters and follow-up data.Methods A prospective analysis of 49 consecutive patients that underwent a OLIF between November 2014 and March 2016 by corresponding author was performed.Only those patients that were single level,index surgeries were included.Every patient had a diagnosis of degenerative lumbar diseases including lumbar spondylolisthesis (25 cases),discogenic low back pain (14 cases) or segmental instability (10 cases).Patients underwent an indirect decompression and fusion using an expandable tubular retractor and single intervertebral cage with bilateral percutaneous pedicle screw fixation.49 patients were divided into the A group (the first 24 patients) and the B group (25 patients after the initial 24 patients).The following data were compared between the two groups:surgical time for Skin-Skin (minutes),estimated blood loss (ml),radiograph exposure time (seconds),the clinical and radiographic results,and intra-/postoperative complications.All intraoperative parameters only included the measurement and findings related with the OLIF procedure.The short-term clinical outcome of single level degenerative lumbar diseases treated by OLIF was assessed on the basis of follow-up data.The learning curve was measured using a logarithmic curve-fit regression analysis.Results Average operative time was significantly longer in the A group 47.1±10.6 min compared with the B group 37.2± 10.0 min.In comparison with the B group,the A group had significantly more X-ray exposure time (25.3±6.1 s versus 17.1±6.9 s).The operative and X-ray exposure time gradually decreased as the series progressed,and an asymptote was reached after about 20 cases.There was no statistically significant difference in intraoperative blood loss between the A group (28.1± 18.2) ml and the B group 24.4± 10.9 ml.The most observed complication was donor site pain (11 cases,45.8%),followed by thigh numbness/pain (5 cases,20.8%) and psoas/quadriceps weakness (2cases,8.3%),paralytic ileus (one case,4.2%) and sympathetic nerve injury (one case,4.2%) in the A group.Donor site pain occurred in four patients (16.0%),thigh numbness/ pain in three patients (12.0%),psoas/quadrieeps weakness in one patient (4.0%) and sympathetic nerve injury in one patient (4.0%) in the B group.All complications were transient and resolved within 3 months.The incidence of complications excluding donor site pain in the early period (A group) and the later period (B group) was 37.5% and 20.0%,respectively,although there were no significant differences in perioperative complications between both groups.Forty nine patients were followed up for more than 1 year,and the average follow-up period was 18.5±3.9 months.The back pain VAS and ODI scores decreased respectively from 6.4±2.3 before surgery to 1.5±0.9 in final follow-up and from 37.1 ±9.4 before surgery to 11.8±3.9 in the last follow-up time.Total fusion rate was 89.8% (44/49 cases)in final follow-up.Radiographic evaluation showed similar bony fusion in the A group (22 of 24 cases) with the B group (22 of 25 cases) in the last follow-up time.Conclusion Single level degenerative lumbar diseases can safely and effectively be treated by using OLIF with a good short-term clinical outcome.The procedure presents a learning curve to the practicing spine surgeon with regards to operative time,X-ray exposure time and intra-/postoperative complications.Intraoperative parameters improved with understanding the minimally invasive technique.Close attention to details can minimize complications that may be associated with the learning curve.
10.Detection of Gene Mutation and Relationship between Phenotype and Genotype in Four Chinese Families with Osteogenesis Imperfecta
yong-cheng, GUO ; shen-jie, CHENG ; yu, YUE ; jian-wei, SHI
Journal of Applied Clinical Pediatrics 2004;0(08):-
T mutation was detected in the 4th propositus at the 9th intron,but any COL1A1 or COL1A2 gene mutation was detected in the third propositus and the other members in the former families.Conclusions The genetic mutation of COL1A1 may result in OI in China,but other mutations may also exist.Moreover,the phenotype was influenced not only by OI genotype,but also by the genetic background,environment and other factors.