To evaluate the value of detection of DNA aneuploidy in exfoliated airway epithelia cells of sputum specimens by the automated image cytometry for the identification of lung cancer, 100 patients were divided into patient group (50 patients with lung cancer) and control group (30 patients with tuberculosis and 20 healthy people). Sputum was obtained for the quantitative analysis of DNA content of exfoliated airway epithelial cells with the automated image cytometry, together with the examinations of brush cytology and conventional sputum cytology. Our results showed that DNA aneuploidy (DI>2.5 or 5c) was found in 20 out of 50 sputum samples of lung cancer, 1 out of 30 sputum samples from tuberculosis patients, and none of 20 sputum samples from healthy people. The positive rates of conventional sputum cytology and brush cytology were 16% and 32%, which was lower than that of DNA aneuploidy detection by the automated image cytometry (P<0.01, P>0.05). Our study showed-that automated image cytometry, which uses DNA aneuploidy as a marker for tumor, can detect the malignant cells in sputum samples of lung cancer and it is a sensitive and specific method serving as a complement for the diagnosis of lung cancer.
*Aneuploidy ; DNA, Neoplasm/*analysis ; Image Cytometry/methods ; Image Processing, Computer-Assisted ; Lung Neoplasms/genetics ; Lung Neoplasms/*pathology ; Sensitivity and Specificity ; Sputum/*cytology

The character of intensive care unit(ICU) makes ICU a chlinical teaching base and plays an important role in medical teaching.By learning from ICU,students can understand and remember basic medical curriculum easily,form their global concept,cultivate their clinical thinking habit,promote their ability of emergency treatment,culture their idea of innovation,and help theim to revere life which is thought as the central idea of medical moral principles.It is worth paying attention to strengthen ICU construction and to properly utilize teaching resource of ICU.

Objective Cloning of some specific genes related to the prostate cancer. Methods Specimen from a patient with prostate cancer and from a normal adult were studied by the improved mRNA differential display and the differentially expressed sequence-tags were cloned,sequenced and analized.Reverse transcriptive-polymerase chain reaction(RT-PCR) was used to examine the expression level of Clusterin in prostate cancer tissues,prostate cancer cell line and normal prostate tissues. Results Significant difference was observed between the two kinds of tissues in gene expression and seven differentially expressed sequence-tags were found through analysis in GenBank.Among them,five were proved to be new gene tags,one shared 100% homology with Clusterin,which was highly expressed in the prostate cancer tissue and the other one shared 97% homology with superoxide dismutase 1 (SOD1),which was highly expressed in the normal prostate tissue.It was proved by RT-PCR that to compare with the internal marker gene ?-actin,the expression level of clusterin in prostate cancer is much higher than that of normal prostate. Conclusions Five new gene tags,clusterin and SOD1 were found to be differentially expressed between tissues of prostate cancer and normal prostate and may play important roles in carcinogenesis and development of prostate cancer.

Herbicides ; poisoning ; Humans ; Paraquat ; poisoning ; Poisoning ; complications ; therapy

Precision medicine is currently being paid much attention and greatly promotes the diagnosis and treatment of various diseases,especially cancer.Surgery plays a pivotal role in the treatment of hepatocellular carcinoma (HCC) and precision hepatectomy stands as the most updated surgical idea.Application of the concept and techniques of precision medicine to further improve HCC surgery,consummate the skills and modalities of HCC surgical treatment,enrich the nature of precision hepatectomy,so as to make precise evaluation,early prediction,reduce postoperative recurrence and metastasis,find biomarkers of molecular targeted therapy and improve the prognosis is the prime task of HCC surgery.

0.05).Lymph node metas- tasis,macroscopic vascular invasion,TNM classification,Edmondson pathologic classification,tumor location and capsule,carbohydrate antigen 19-9 (CA19-9) might affect the outcome post-LT.Conclu- sion The overall survival of LT for CCC was dismal.We should select the recipients very carefully and strictly.Those CCC patients with large tumor,TNM class-Ⅲ,bi-lobe distribution or who can not be ruled out lymph node or vascular invasion,should be contraindicated for LT.

Objective To reevaluate Shanghai Criteria of liver transplantation (LT) for hepatocellular carcinoma (HCC).Method We evaluated the outcome of 433 consecutive patients with HCC who underwent LT from 2001 to 2013 at Zhong shan Hospital,Fudan University.The Kaplan Meier product-limit method with log rank test was used to evaluate survival rate according to different Criteria.Result The 5-year overall survival (OS) rate and relapse-free survival (RFS) rate were as follows:Milan Criteria (n =169; 72.5％ and 79.0％),UCSF Criteria (n =223; 68.2％ and 75.7％) ;Shanghai Criteria (n =255; 65.9％ and 70.6％) ; Pittsburgh Criteria (n =419,55.6％ and 61.2％).There was no significant difference in the 5 year OS and RFS between Shanghai Criteria group and Milan Criteria group (P＞0.05).Conclusion Shanghai Criteria,which modestly expanded current criteria while still preserving similar long term survival,could be more fit for the situation of China.

Whether the sentinel lymph node (SLN) metastasis has important clinical significance for the therapy and prognosis of tumor. Sentinel lymph node biopsy (SLNB) has became the most accurate clinical method to confirm the status of sentinel lymph node. And the key of the success of SLNB is the localization of SLN. The methods used to locate SLN mainly are blue dye tracer method, radionuclide tracer technique, blue dye tracer method combined with radionuclide tracer technique, indirect lymphography, NIR imaging, and photoacoustic imaging. This article demonstrates the principle, application method and contrast agents of the indirect lymphography and the application in head and neck tumor.
Contrast Media ; Head and Neck Neoplasms ; diagnosis ; Humans ; Lymph Nodes ; Lymphatic Metastasis ; Lymphography ; Prognosis ; Sentinel Lymph Node Biopsy

Objective To observe the effect of intact vagus nerve stimulation(IVNS)on liver inflammation and mRNA expression of suppressors of cytokoine signaling(SOCS)in rats. Methods Eighty Sprague Dawley rats were randomly divided into control group(n=20),sham group(n=20),LPS group(n=20,with intravenous injection of LPS to induce system inflammatory response)and LPS +IVNS group(n=20,with intact vagus nerve stimulation after LPS administration).The level of TNFαand IL-10 in the liver was detected with enzyme linked immunosorbent assay at 0,2,4,6 hours post-injection time.mRNA expressions of SOCS1 and SOCS3 in the liver were also determined with reverse transcription and polymerase chain reaction(RT-PCR). Resuits The liver TNFαin LPS+INVS group was lower than that of LPS group after LPS injection at 2,4 and 6 hours(P＜0.05).Compared with LPS group,liver IL-10 level in LPS+IVNS group was higher(P＜0.05)at post-injection time points(4 and 6 hours).After four hours of LPS challenge,mRNA expressions of both SOCS1 and SOCS3 were significantly increased.Compared with LPS group,mRNA expression of SOCS3 in LPS+INVS group was upregulated more significantly(P＜0.01),while mRNA expression of SOCS1 showed insignificant difference. Conclusions IVNS can depress liver inflammation and the anti-inflammatory mechanism involves SOCS signal transduction pathway.

Objective To investigate the cytokine spectrum of cultured human amniotic-derived mesenchymal stem cells (A-MSC) for understanding its basis of molecular biology in hematopoietic in vitro.Methods Their hematopoietic cytokines expression was analyzed using RT-PCR in the mRNA level. Results It showed that in vitro subcultured human amniotic-derived mesenchymal stem cells were capable to express many important hematopoietic cytokines such as LIF, SCF, M-CSF, G-CSF, GM-CSF, IL-3, IL-6, IL-11 and so on. Conclusion Production of abundant of hematopoietic cytokines by human amniotic-derived mesenchymal stem cells may be effective for hematopoietic support and HSC transplantation.