Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Chronic Disease ; therapy ; Female ; Humans ; Male ; Middle Aged ; Pterygopalatine Fossa ; innervation ; Rhinitis ; physiopathology ; therapy ; Smell ; Treatment Outcome ; Young Adult

BACKGROUND:Recent advances in polylactic acid-polyethylene glycol (PLA-PEG) block copolymer and hydroxyapatite surface-grafted with poly(l-lactide) (HA-g-PLLA), including their synthesis and preparation, have been approved for application in bone tissue engineering. But they have stil many disadvantages. Combination of HA-g-PLLA and PEG-PLA could show a great potential for development in bone tissue engineering. OBJECTIVE:To investigate the effect of PLA-PEG/HA-g-PLLA composite fibrous material with different content of HA-g-PLLA on adhesion, multiplication and cellviability of osteoblasts. METHODS:Four fibrous materials of 5%PLA-PEG, 5%PLA-PEG/5%HA-g-PLLA, 5%PLA-PEG/10%HA-g-PLLA, 5%PLA-PEG/15%HA-g-PLLA were prepared using electrospinning technique and divided into four experimental groups. Mouse osteoblasts were inoculated on the materials. A statistical analysis was done based on results from celldyeing and alkaline phosphatase detection at certain time points to evaluate the effect of new materials on osteoblast adhesion, proliferation and viability. RESULTS AND CONCLUSION:In the experiment of cellmultiplication and the test of alkaline phosphatase activity, both absorbance values and activity of alkaline phosphatase from high to low were 5%PLA-PEG/10%HA-g-PLLA>5%PLA-PEG/15%HA-g-PLLA>5%PLA-PEG/5%HA-g-PLLA>5%HA-g-PLLA (P<0.05). The absorbance value of PLA-PEG/HA-g-PLLA roughly presented a waveform with the increasing addition of HA-g-PLLA. The effect of PLA-PEG/HA-g-PLLA composite fibrous material on adhesion, multiplication and osteogensis of osteoblasts increases with addition of HA-g-PLLA in a certain range.

Objective:To culture canine dental pulp stem cells(cDPSCs)in vitro.Methods:Canine pulp cells were isolated and cultured by enzyme digestion and explanted tissue culture respectively.Cell morphology was observed under phase-contrast micro-scope.The clone forming unit(CFU)of the cells was examined by plate clone formation assay.Cell markers and protein-expression were examined by flow cytometry(FC)and immunofluorescence.Odontogenic and adipogenic potential were evaluated by alizarin red staining and oil red O staining.Results:Short spindle fibroblast-like and steadily growing cells were obtained by both methods.The clone assay showed that CFU was 1 5.1 7% ±2.79%.FC observasion showed that the CD90,STRO-1 and CD24 positive cells were 24.43% ±7.1 0%,20.67% ±1 .42% and 2.03% ±0.06% respectively,but CD34 was negative.Immunofluorescence analysis showed positive expression of Nestin,Vimentin,weak expression of ALP and negative expression of DSP of the cells.Differentiation ex-periment confirmed the odontogenic and adipogenic differentiation potential of the cells.Conclusion:cDPSCs can be cultured in vitro.

Objective To investigate the clinical results of bilateral transpedicular kyphoplasty for the osteoporotic vertebral compression fractures with single balloon. Methods From March 2002 to February 2004, percutaneous bilateral transpedicular kyphoplasty with single balloon was performed in 15 patients, who presented with painful vertebral compression fractures. In the group, there were 6 vertebrae of 6 males and 11 vertebrae of 9 females, and the average age of the patients was 70.5 years with a range of 62 to 82 years. 17 osteoporotic vertebral compression fractures, which involved 8 T12, 7 L1, 1 T8 and 1 T11, were treated. Osteoporosis was identified in all cases by the measurement of lumbar bone mineral density (BMD) and the evaluation of radiographs. The average BMD was 0.603 g/cm2. CT scan showed the posterior vertebral wall intact in all patients, and MRI displayed low signal on T1WI and high signal on T2WI of the vertebral fractures. Results All patients experienced dramatic pain relief after the procedures. The mean pain score (VAS) decreased significantly from 8.3 preoperatively to 2.1 after operation. The mean vertebral body height restoration was 54.2%?34.1% on the anterior border, 60.5%?35.4% in the middle portion, and 40.7%?32.3% on the posterior border respectively. And the sagittal alignment was improved significantly, as the average correction of the Cobb's angle reached 9.5?. No cement leakage was found on X-ray, except one with less cement at the anterior vertebral edge, but no clinical symptom occurred. All the patients were available at the final follow-up, the mean period was 10.5 months (range, 2-48 months). Conclusion Bilateral transpedicular kyphoplasty with single balloon not only can provide effective pain relief and vertebral height restoration, but also can improve the sagittal alignment significantly. Its clinical outcome was satisfactory.

Diagnosis related groups (DRGs)have been widely used in many countries and regions.The versions of DRGs in different regions are modified according to the local circumstances and conditions.Beijing version DRGs (BJ-DRGs)is the first DRGs system in Chinese,which was developed locally based on the conditions of medical information system and health relative policies in Beijing.This paper introduced the grouping principles,logic and methodology of BJ-DRGs.Taking“ear,nose,mouth and throat disorders”for example,this paper demonstrated the grouping process of BJ-DRGs.

OBJECTIVETo observe the effect of external application of Zhuangshenling Recipe (ZR) combined with Western medicine (WM) on the heart function of chronic heart failure (CHF) patients of Xin-Shen yang deficiency, interior retention of water-fluid syndrome (XSYDIRWFS).

METHODSTotally 140 CHF patients of XSYDIRWFS were randomly assigned to two groups, the treatment group and the control group, 70 in each group. All patients received WM therapy. Those in the treatment group were applied with ZR at Xinshu (BL15) and Shenshu (BL23), while those in the control group were applied with placebos at Xinshu (BL15) and Shenshu (BL23). The therapeutic course for all was 12 weeks. The integrals of TCM syndrome, grading of cardiac function, brain natriuretic polypeptide (BNP), and 6 min walking distance were observed before and after treatment.

RESULTSAfter twelve weeks of treatment, the effective rate of improved grading of cardiac function, the total effective rate of TCM syndrome efficacy, and the BNP level were obviously better in the treatment group (P < 0.05). There was no statistical difference in 6 min walking distance between the two groups (P > 0.05).

CONCLUSIONExternal application of ZR combined with WM could improve the heart function of CHF patients of XSYDIRWFS.

Aged ; Chronic Disease ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Heart Failure ; drug therapy ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Yang Deficiency ; drug therapy

Human lymphocyte function-associated antigen 3 (hLFA3) has been identified as an important T cell accessory molecule. Rhesus monkeys (Macaca mulatta) have been widely used as animal models for human immune disorders. Due to the species-specificity of immune system, it is necessary to study M. mulatta LFA3 (mmLFA3). In this study, the gene encoding mmLFA3 CD2-binding domain (mmLFA3Sh) was amplified by polymerase chain reaction (PCR) and genetically fused to human IgG1 Fc fragment in pPIC9K to construct the expression plasmid pPIC9K-mmLFA3Sh-Ig. Approximately 3-4 mg mmLFA3Sh-Ig protein was recovered from 1 L of inductive media, and mmLFA3Sh-Ig produced by the P. pastoris can bind to the CD2 positive cells, and suppress the monkey and human lymphocytes proliferation induced by Con A and alloantigen in a dose-dependent manner. These results suggested that mmLFA3Sh-Ig might be used as a novel tool for pathogenesis and experimental immunotherapy of Rhesus monkey immune disorders.
Animals ; CD58 Antigens ; biosynthesis ; Humans ; Immunoglobulin G ; Lymphocyte Activation ; Macaca mulatta ; Pichia ; Plasmids ; Protein Interaction Domains and Motifs ; Recombinant Fusion Proteins ; biosynthesis ; T-Lymphocytes

Objective To explore the relationships between erythropoietin( EPO), endothelin - 1 (ET - 1) and perinatal anoxia. Method ELISA was used to test cord blood EPO and ET-1 in 54 high risk neonates as subjects and 14 healthy neonates as controls.Results The cord blood EPO levels in amniotic fluids turbid Ⅲ degree group and group eclampsia/pre - eclampsia were higher than those in control group (t= 4.0842,3 680 allP

Objective To synthesize glycylproline p-nitroanilide tosylate as a substrate for glycylprolyl dipeptidyl-aminopeptidase(GPDA),and to study its clinical application.Methods The title compound was prepared by DCC-HOBt synthesis.GPDA in human serum was detected by continuous monitoring method using substrate. Results The substrate with purity of 98.5% was obtained.The linearity of the method was up to 358.1 U/L.Intraassay CV and interassay CV of GPDA in diverse serum samples were 3.01% and 5.04%,respectively.It was shown that GPDA level in patients with hepatic cancer was significantly increased,while those in patients with gastric carcinoma and chronic gastritis were significantly decreased compared with that in the control group in clinical detecting(P