OBJECTIVE: To investigate the possible beneficial effects of administration of Naoluo Xintong Recipe (NLXT) on the expression of HSP70 and bFGF in rats subjected to ischemia-reperfusion with qi deficiency and blood stasis syndrome. METHODS: We blindly randomized 40 male Wistar rats into 5 groups which were categorized as follows: (1); sham-operated group; (2) rats subjected to transient middle cerebral artery (MCA) occlusion with qi deficiency and blood stasis syndrome (control group); (3) NLXT-treated group; (4) NLXT-pretreated group; (5) Nao'an Capsule-treated group. Focal brain ischemia was induced by the intraluminal suture MCA occlusion method after qi deficiency and blood stasis syndrome had been made. The rats' brains were harvested at the 24h of reperfusion after 2h of MCA occlusion. The expression of HSP70 and bFGF was measured by by immunohistochemical staining. RESULTS: Strong HSP70 immunoreactivity in neurons was observed in NLXT-treated and NLXT-pretreated groups as compared to control group, and increased bFGF immunoreactivity in neurons was observed in the ischemic hemisphere in NLXT-pretreated group in comparison with the ischemic hemisphere in the control group (P<0.01). NLXT has stronger effects on HSP70 and bFGF than Nao'an Capsule. CONCLUSION: The neuroprotective effects of Naoluo Xintong Recipe may be related to its up-regulation of HSP70 and bFGF in the rat brain after focal cerebral ischemia.

The immune nutrients play an important role in nutritional support,immune regulation and maintenance of intestinal barrier function.In recent years,with the deepening study,the effect of immune nutrients in the comprehensive cancer treatment has constantly being recognized.Many studies have shown that glutamine (Gln),arginine (Arg) and polyunsaturated fatty acids (PUFA) have effect on the tumor itself and the combination cancer chemotherapy.

Objective To evaluate the microRNA role in the pathogenesis of alcohol dependence.Methods By computer retrieval Medline,mirbase,PubMed,Chinese journal full-text database (CNKI),ten thousand database and VIP database,manual retrieval relevant references.Literature retrieval time limit are built from the library until July 2014.English database to Alcoholism,Alcohol Dependence,Alcohol Abstinence,Alcohol Amnestic Disorder Fetal Alcohol Spectrum Disorders,mirna as keywords retrieval;Chinese database to Alcoholism,Alcohol Dependence,Alcohol Abstinence,Alcohol Amnestic Disorder Fetal Alcohol Spectrum Disorders,microRNA,etc.Retrieved from the database to the related literature in July 2014.Results A total of 26 studies were recruited including 8 Chinese literatures and 18 English literatures.Conclusion Abnormal expression of miRNAs will become the next research direction.MicroRNAs may become new biomarker of alcohol dependence on diagnosis,may also makes the molecular drug targets,or the molecular simulation for new drug research and development,this could be for the treatment of alcohol dependence provides a new means.

0.05), but after 21 days of different estrogen treatment when the different estrogen levels were stabilized there were significant differences in PWL among the 3 groups ( P

85%).From the in vitro release experiment,the rhBMP-2 was controlled to release from the chitosan microsphere over 30 days.[Conclusion]The rhBMP-2 loaded chitosan microsphere prepared by emulsion cross-linking method shows a good controlled release property.Such a novel type of controlled release system has the potential of being applied for controlled cytokine delivery and the bone tissue engineering.

Objective:The pathogenesis of placental vascular disease(PVD) was further investigated. Methode:PVD group (study group) and normal pregnancy (control group) were recruited. Two-channel mi-croarray technique was applied to screen the most differently expressive genes of human genome, and veri-fied the different expressions in mRNA and protein levels by quantitative PCR and Westem-blot, also local-ized the expression of different genes in placental tissue by immunohistochemistry. Reeults:HspA6 and Hs-pA1A had extremely higher expression in placentas with PVD than normal pregnancy. These genes both en-coded Hsp70. Hsp70 was mostly expressed in endothelial and smooth muscle cells of placental microves-ssls. The expression of Hsp70 mRNA and protein were up-regulated in placental tissue and microvascular endothelial cells from PVD group compared with normal pregnancy ( P <0.05) ;The expression of Hsp70 mR-NA and protein had negative correlation with infant birth weight ( P < 0.05). Conclueione:Hsp70 was associ-ated with endothelial activation in the pathogenesis of PVD by initiating an innate immune response and in-flammatory reaction.

Objective To evaluate safety and validity of 3D video-assisted thoracoscopic technology in cardiac surgery.Methods Between October 2013 and July 2014,50 patients underwent 3 D Video-assisted thoracoscopic cardiac surgery including:5 atrial septal defect closure,1 ventricular septal defect closure,repairment of 1 unroofed coronary sinus,1 partial atrioventricular septal defect and 1 cor triatriatum,28 mitral valve replacement (1 re-MVR),1 tricuspid valve replacement,9 mitral valvuloplasty,2 left atrial myoxoma resection,and 1 left internal mammary artery harvest + minimally invasive direct coronary artery bypass.All procedures were performed under extracorporeal circulation,using 30° thoracoscope and 3D high resolution monitor.Results There was no perioperative death.One patient was converted to median sternotomy because of pericardial adhesions.Mean operative time was(232.8 ± 54.7) min,mean cardiopulmonary bypass time was(128.0 ±42.5) min,mean aortic cross-clamp time was (74.7 ± 25.1) min.Mean ICU stay was (30.4 ± 22.0) h,mean duration of mechanical ventilation was(13.7 ± 11.9) h.Mean postoperative drainage time was (3.34 ± 1.91) d,mean hospital stay was (6.52 ± 3.06) days.There were 11 (22％) operative complications,including 4 intrathoracic bleeding,1 wound infection,3 hemothorax,1 brachial plexus injury,1 intractable hiccup and 1 mild paravalvular leakage.Compared with 20 mitral valve replacement by 2D thoracoscopy,the 3 D group was slightly less time consuming(P ＞ 0.05).Conclusion 3 D video-assisted thoracoscopic cardiac surgery is feasible and safe,and it' s worthwhile to promote this promising technology.

Adult ; Coronary Artery Disease ; complications ; etiology ; Female ; Heart Valve Prosthesis ; adverse effects ; Humans ; Mitral Valve ; surgery ; Myocardial Infarction ; etiology ; Postoperative Complications ; Thromboembolism ; complications ; etiology

Brain ; diagnostic imaging ; pathology ; Cerebral Palsy ; diagnosis ; pathology ; Cognition Disorders ; diagnosis ; pathology ; Early Diagnosis ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; diagnosis ; pathology ; Language Disorders ; diagnosis ; pathology ; Leukomalacia, Periventricular ; complications ; diagnosis ; pathology ; Magnetic Resonance Imaging ; methods ; Prognosis ; Radiography ; Severity of Illness Index

Objective:To investigate the existence of side population cells with the potency of stem cells in human gallbladder carcinoma cell line GBC-SD and the differences in ABCG2,Oct-4 and CD34 expression among SP cells,non-SP cells and GBC-SD cells.Methods:SP and non-SP cells were sorted from GBC-SD cells by fluorescence-activated cell sorting(FACS).The expression of ABCG2,Oct-4 and CD34 in SP cells,non-SP cells,and GBC-SD cells was detected by reverse transcription-polymerase chain reaction(RT-PCR),Western blot,flow cytometry(FCM)and immunofluorescence chemistry.Results:SP cells with stem cell potency were isolated from GBC-SD cells with a proportion of(0.64±0.08)%.The metastatic ability of SP cells was obviously higher than that of non-SP cells and GBC-SD cells(P<0.05).The expression of ABCG2 was significantly higher in SP cells than in non-SP cells and GBC-SD cells[(89.56±3.86)%vs.(1.32±0.49)%and(12.37±1.61)%,P=0.001].The expression of Oct-4 in these cells was(94.87±1.40)%,(88.16±2.34)%and(90.17±1.61)%,respectively(P>0.05).CD34 was neady absent in these cells on protein level[(1.78±0.51)%vs.(0.63±0.21)%and(0.96±0.381)%,P>0.05)],but it was highly expressed in non-SP cells and GBC-SD cells and absent in SP cells off mRNA leve;.Conclusion:SP calls which hava the potency of stem cells,exist in human gallbladder carcinoma GBC-SD cell line and have the phenotype of ABCG2+Oct-4+CD34-.