Objective:To evaluate the clinical value of MR cholangiopancreatography(MRCP) as the only imaging procedure in diagnosing biliary complications after orthotopic liver transplantation(OLT).Methods: The MRCP results were analyzed in 54 consecutive patients presented with clinical or biochemical signs of biliary complications after receiving OLT,and were compared with the findings eventually confirmed by surgery,direct cholangiography,and clinical follow-up.Two different MRCP snapshot techniques were applied: thick-slab T_(2)-weighted sequences and multi-section thin-slab T_(2)-weighted sequences.Results: Surgery,direct cholangiography,and clinical follow-up confirmed that 36 patients had biliary complications.MRCP had a sensitivity of 100%(36/36) in diagnosing biliary complications after OLT,with a positive predictive value of 91.7%(33/36),a false-positive rate of 5.56%(2/36),and an overall diagnostic accuracy of 94.4%(51/54).MRCP as the only imaging procedure achieved a specific diagnosis in 96.3%(52/54) of the patients in the present study.Only 2 patients required additional endoscopic retrograde cholangiopancreatography and percutaneous transhepatic cholecystostomy.Direct cholangiography was required only as a therapeutic procedure in 25.9%(14/54) of the patients.Conclusion: MRCP is an effective imaging procedure in the assessment of biliary complications after OLT.Direct cholangiography is not needed for diagnosis but should be reserved as a therapeutic procedure.

retina. Degeneration was found in the epithelial cells of lens at day 30 after radiation. During day 90 to 360 after radiation, lens capsule was thickened, degeneration of epithelial cells, and edema on the posterior surface were found, and cataract ensued. The injurious effect was dose-related. Conclusion High power EMP may cause posterior capsular opacity and subsequent cataract, and the injurious effect was dose-related.

Objective To analyse the clinical characteristics,gene mutation and enzymatic activity of αgalactosidase A(α-GalA)in a 15-year-old male patient with typical Fabry disease,whose mother was without any clinical manifestations.Methods Clinical features and laboratory data were collected from the patient and his mother.Genomie DNA was extracted from peripheral blood of the patient.his mother,and a healthy control subject.Seven exons of the GLA gene were amplified by PCR.PCR products were purified.cloned into T vector,and then sequenced.The enzymatic activity of α-GalA Was measured by fluorimetrie substrate assay. Results DNA sequencing results showed that a missense mutation of 10036-10038delAAG in exon 7 WaS identified in the patient,resulting in the replacement of 374 lysine and 375 glyeine by arginine,which Was not previously reported.The patient Was a hemizygote with gene mutation,his mother WaS a heterozygote carrying gene mutation,and the healthy control without mutation.α-GalA enzymatic activity assay showed that the enzymatic activity of the patient with GLA gene mutation was only 50%of the healthy control subject,while the enzymatic activity of the patient's mother Was about 70%of the heahhy control SObject.Conclusiolls Detecting GLA gene mutation and α-GalA enzymatic activity in patients with Fabry disease who have been clinically diagnosed seelns to be helpful in finding other patients in the family and in further understanding the molecular pathogenesis of that disease.

To investigate the inhibitory effects of RNAi expression vector on CaMKⅡ? expression of PC12 cell lines. siRNA expression vectors were constructed to be targeted directly at CaMKⅡ? gene. The recombinants were transfected into PC12 cell line with liposome. The expression of CaMKⅡ? was detected by RT-PCR and Western blot. Expression vectors could reduce the expressions of CaMKⅡ? mRNA and protein in PC12, Compared with blank vector group, the former, the ratio of inhibition of the expression of CaMKⅡ? mRNA was 46.40% and 64.69%, in 48th hour and 72th hour, the ratio of inhibitory of the expression of CaMKⅡ? protein was 74.77% in 72th hour. RNAi expression vectors can effectively inhibit the expression of CaMKⅡ? in PC12.

A simple,reliable RPHPLC method on ODS column with UV detector for deter-mination of the anticoagulant rodenticide coumatetralyl in animal tissue was developed.1,1′-Bi-(2-Naphthol)was used as an internal standard to check the process ofexperiments.The mean recoveries from the spiked rabbit liver were around 90% atthe levels of 0.4-16mg/kg.The coeffecint of variation of 5 determination was 3.2%.The results showed that this method has a satisfactory reproducibility.The methodis practical for examining poisons in poisoning cases.

Nine Rabbits were orally administrated with a single dose of 25mg/kg,100mg/kgcoumatetralyl to establish poisoning model.Poisoning sympton and pathological changes were observed.Coumatetralyl content in main internal organsand muscle was determined by RPHPLC method.The results were discussed.

AIM:To prepare the Lyophiled Royal Jelly Soft Capsule and study its stability and Influential factors.METHODS:The suspending agent and processing method were optimized using sedimentation volume rate as the index.Soft capsules were prepared and product stability under high temperature and high humidity environment was studied according to the determination of the content of 10-HAD by HPLC.RESULTS:The finished product yield in pilot test was more than 90%,the soft capsule products stored in cold were stable,while those stored under room temperature or high temperature and high humidity were unstable with a noticeable decrease in quality.Water content in capsule shell affects the 10-HDA content of the finished product.CONCLUSION:The preparative process is feasible and the products should be storaged in cold enviroment.

Objective To study the effects of chickpea protein hydrolysate(CPH) in different doses on of antioxidant abilities in serum,liver and heart in aged mice induced by D-galactose .Method The subacute aged model mice were made by neck back subcutaneous injection of D-galactose continually.The content of malondialdehyde(MDA) and activity of superoxide dismutase(SOD) and glutathione peroxidase(GSH-PX) in the serum,liver and heart of the mice were tested.Results The SOD and GSH-Px activities in aged model group were significantly decreased and MDA significantly increased compared with normal mice;CPH in different doses evidently increased the SOD and GSH-Px activities and reduced the MDA level in serum,liver and heart in mice.Conclusion CPH can obviously increase the antioxidant ability of mice and has certain anti-aging effects,especially the high dose .

Objective To study the condition of postoperation complications in patients with supratentorial cerebral hemisphere gliomas,and to provide objective references for clinical classification and treatment. Methods Data of 100 patients with supratentorial cerebral hemisphere gliomas,treated surgicaly in the department of neurosurgery,gliomas center of Tiantan hospital from June 2006 to December 2007,were reviewed and analyzed.We classified postoperative complications as neurological,regional and systemic complications,and especially mainly analyzed the reasons of oceching,prevention and cure of the neurological postoperative complications.Results Through the complete preoperative evaluation in patients to choose the proper operative route,methods and technique means can improve postoperative KPS score compare with preoperative obviously ,which can achieve better operation result.Conclusion Neurosurgeon must design consummately individualization operation plan depending on patient history,neurology symptoms,physical sign,and preoperative check,and understand the operation anatomy including the pathology anatomy and function anatomy. They must foresee the postoperative complications,prevent it as far as possible,and treat it reasonable in time.

Objective To investigate effect of lipid emulsion on cardiac arrest induced by bupivacain intoxication.Methods Forty SD rats were randomly devided into 2 groups.Group A applied epinephrine (n=20);Group L applied lipid emulsion combinding epinephring(n=20).The rats were administered Lupivacaine 20 mg/kg.Cardiopulmonary resuscitation(CPR) was started. Epinephrine 10 μg/kg were repeated three times followed by epinephrine 10 μg/kg every 5 minutes.Normal saline 5 ml/kg and 1 ml·kg-1·min-1 were administered in group A.20% Lipid emulsion 5 ml/kg and 0.25 ml·kg-1·min-1 were administered in group L.Heart rate,blood pressure, temperature were monitored.Blood gas analysiswere checked at 5 min,30 min after restoration of spontaneous circulation(ROSC).To record the number of successful CPR,time of ROSC and epinephrine dose.Results 11 rats(55%) and 16 rats(80%) were successful resuscitated.The success rate was higher in group L than in group A.Epinephrine dose was higher in group A than in group L.There was no difference in heart rate between the two groups.Systolic blood pressure and PaO2 was higher in group L than in group A(P<0.05).Conclusion There was better effect in lipid emulsion combined epinephrine than soly epinephrine when applied in cardiac arrest induced by bupivacaine intoxication.