1.Epidemiological Analysis and Surveillance System Evaluation for Nosocomial Infections
Yuanhui LI ; Jieyu CHEN ; Jian RU ; Yuxiu QIN ; Qiumei LIAO
Chinese Journal of Nosocomiology 2006;0(09):-
OBJECTIVE To analyze the epidemiological characteristics,the efficiency of the surveillance system for nosocomial infections in our hospital,the sensitivity,specificity and timeliness of case reporting and to evaluate the efficiencies of the monitoring and reporting system.METHODS We reviewed the nosocomial infection reporting data in the year of 2005 and identified all the reported cases,confirmed cases and under-reported cases.Sensitivity and specificity of the reporting system were calculated based on these data.RESULTS The incidence of nosocomial infection was 3.04% in 2005.A majority(72.07%) of the cases were reported within 7 days of occurrence.Respiratory tract infection accounted for 57.75% of all nosocomial infections and was by far the most common site of concern.The rates of false positive reporting ranged from 14-37% and were statistically significantly associated with the sites of the infection(P
3.Electromyographic analysis of upper limbs and trunk during elbow extension activities in C_5 and C_6 SCI patients
Ying LIU ; Jian-Jun LI ; Gui-Ru HUA ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(11):-
Objective To explore how the spared muscles in upper limbs and trunk compensated for elbow extension movements in patients with SCI at the level of C_5 and C_6.Methods Fifteen patients with SCI at the level of C_5 and C_6 and fifteen healthy subjects were divided into an SCI group and a control group respectively.The surface EMG recorded from the anterior dehoid,middle deltoid,posterior deltoid,triceps braehii muscle,biceps braehii muscle,upper peetoralis,serratus anterior and latissimus dorsi during four elbow extension activities including grasp- ing cup,reaching for light-switch,wheelchair ambulation and upper limb weight-bearing.The relative EMG intensi- ties of muscles were expressed as a percentage of maximal voluntary contraction.Results Several muscles partici- pated in the activity simultaneously when both groups of subjects performed every motor task.But the primary muscles involved in the movements were different between the two groups.Furthermore,the SCI group tended to employ more muscles than the controls to perform a activity.The compensatory strategies used in various motor tasks by the spared muscles were different.Conclusion The motorueuron recruitment patterns used by the C_(5~6)SCI patients in per- forming elbow extension activities could be different from those by the healthy subjects.The patients accomplished performing different motor tasks by changing their compensatory strategies.
5.SAS Application in Studies on Medium Optimizationof Lactic Acid Fennenlation
Zi-Jun XU ; Jian LI ; Feng-Lai LIANG ; Jian-Fang MA ; Ru-Lin LIU ;
Microbiology 1992;0(03):-
The optimum medium for Lactobacillus M7 was systematically studied with SAS system. Firstly, the prime factors affecting lactic acid yield were selected by means of Plackett-Burman design; secondly, the pnme facias were optimized by response rurface analysis. Under the optimum level determined, the yield is increased by 15%.
6.Serum B-type natriuretic peptide changes in patients with acute coronary syndrome during percutaneous coronary intervention
Jian LI ; Bing DENG ; Ai-Li LIU ; Ru-Hui LIU ; Li-Qiang ZHENG ;
Chinese Journal of Emergency Medicine 2006;0(10):-
Objective To observe the serum B-type natriuretic peptide(BNP)changes in patients with acute coronary syndrome during percutaneous coronary intervention(PCI).Method The serum BNP level was determined and the heart function was assessed in 236 consecutive patients with acute coronary syndrome(ACS) admitted to CCU and in 54 normal normal suvjects as control from January 2005 to December 2006 in Tongji Hospital.The ACS patients were further divided into various subgroups according to the involved arteries and performance of PCI.Serum BNP,hypersensitive c respose protein(HsCRP)level,amd heart comstitution and function were analyzed.Results The serum BNP and HsCRP level were significantly increased in patients with ACS [(332.06?483.17)ng/L and(31.06?52.15)mg/L]more than those in normal subjects [(81.44?195.55)ng/L and(11.15?20.78)mg/L,respectively,P
7.Neuropsychological study on silent brain infarction
Ming LI ; Jian LI ; Bai-ru HUANG ; Huishun ZHANG ; Weijun LI
Chinese Journal of Rehabilitation Theory and Practice 2002;8(6):364-366
ObjectiveTo investigate the neuropsychological impairments and risk factors that caused cognitive disturbance in patients with silent brain infarction (SBI). Methods36 SBI subjects and 25 normal controls were compared on a comprehensive neuropsychological test battery. Logistic regression was used to analysis the risk factors. ResultsThe SBI group scored lower on the visual retention test (VRT), the WAIS RC full scale IQ, standard scores of the perceptual organization factor and memory/attention factors than did the control group. The score of self rating depression scale (SDS) was higher in SBI group. Hypertension and leukoaraiosis (LA) were correlates of cognitive impairment in logistic regression analyses. Conclusions SBI has some degree of neuropsychological impairment, especially deficits of visuospatial ability, disturbance of perceptual organization, memory and attention, and some degree of depression. Hypertension and LA independently contribute to the risk factors of cognitive disturbance.
8.CLONING OF NATTOKINASE GENE AND EXPRESSION IN E. COLI
Li-Xin LUO ; Zhi-Li HUANG ; Ru-De YANG ; Jun-Jian LING ; Shi-Zhong LIANG ;
Microbiology 1992;0(03):-
In this study, nattokinase gene was amplified by PCR using bacillus subtilis chromosomal DNA as template and cloned into expressed vector pBV220. After transforming recombinant plasmid into E.coli HB101, the recombinant strain was yielded. It was proved that expression products was secretive and expression protein was 12% of total cell protein by SDS-PAGE. Optimum culture time and inducing time was determined as 6h and 5h respectively. The plasmid stability studies showed that recombinant plasmid has excellent segregational stability but the structural stability was not good in the host cell.
9.The relationship between perfusion defects on myocardial SPECT and stenotic severity on CT coronary angiography
Jian-ming, LI ; Rong-fang, SHI ; Ting, LI ; Xiao-bin, ZHAO ; Ru-ming, LU ; Yu, LIANG
Chinese Journal of Nuclear Medicine 2011;31(6):394-399
ObjectiveTo evaluate the diagnostic performance of CTCA in predicting myocardial perfusion defects through comparative analysis between MPI defects and severity of coronary stenosis on CTCA.MethodsFour hundred and seventy-eight patients who underwent CTCA and 99Tcm-MIBI MPI in the same period were analyzed retrospectively.According to the severity of coronary stenosis judged by visual evaluation of the vessel diameter,the patients were divided into five groups:no stenosis,mild stenosis,moderate stenosis,severe stenosis and total occlusion.MPI results were classified as negative or positive for perfusion defects,and the prevalence of perfusion defects in every group was calculated per-patient and per-vessel basis.The cut-off of stenotic severity for predicting myocardial perfusion defects was designated as 50% or 75%,with MPI as standard reference.True positive,true negative,false positive and false negative statistics were thus determined separately on patient and vessel basis.The diagnostic performance for CTCA were calculated and compared.Pearson Chi-square and its partition tests or Fisher exact test were used to compare ordinal variables.ResultsFifty-eight patients showed myocardial perfusion defects.Either by patientbased or vessel-based analysis,the prevalence of myocardial perfusion defects showed an increasing trend with greater coronary artery stenosis in each group,and there were statistical differences among them (x2 =116.62 and 483.83,both P < 0.05).On patient-based analysis,sensitivity ( SN),specificity ( SP),positive predictive value( PPV),negative predictive value(NPV) and accuracy (AC) for CTCA predicting myocardial perfusion defects were 62.1 % ( 36/58 ) and 34.5% ( 20/58 ) (x2 =8.84,P < 0.05 ),84.5%(355/420) and 97.1% (408/420) (x2 =40.16,P <0.05),35.6% (30/101) and 62.5% (20/32) (x2 =7.19,P<0.05),94.2% (355/377) and 91.5% (408/446) (x2 =2.18,P >0.05),81.8% (391/478)and 89.5 % (428/478) (x2 =11.66,P < 0.05 ) when the cutoff was set to 50% and 75%,respectively.On vessel-based analysis,the SN,SP,PPV,NPV and AC for CTCA predicting myocardial perfusion defects were 58.8% (40/68) and 30.9% (21/68) (x2 =10.73,P < 0.05),95.9% (1768/1844) and 99.0% (1826/1844) (x2 =36.72,P < 0.05 ),34.5% (40/116) and 53.8% (21/39) (x2 =4.59,P <0.05 ),98.4% (1768/1796) and 97.5% ( 1826/1873 ) (x2 =4.14,P < 0.05 ),94.6% ( 1808/1912 ) and 96.6% ( 1847/1912 ) (x2 =10.31,P < 0.05 ),respectively.ConclusionsThe prevalence of myocardial perfusion defects correlates positively with the severity of coronary stenosis seen on CTCA.CTCA may predict perfusion defects with high SP and NPV.However,the PPV of CTCA in predicting myocardial perfusion defects is poor when the stenosis cut-off is set at 50%.It is significantly improved when the cutoff value is set at 75 %.
10.A comparative study of the influence of different bowtie of 64 multi-slice CT on cardiac image quality and radiation dosage
Jian-Hua GAO ; Xian-Chang SUN ; Jian-Ying LI ; Na LI ; Qing-Tang XIA ; Wen ZHAO ; Ru-Ping DAI ;
Chinese Journal of Radiology 2001;0(08):-
0.05 indicating no statistical difference.However,the noise measurements for the L and C groups were 30.05 and 27.80,respectively,with P