Cardiomyopathy, Dilated ; pathology ; Humans ; Infant, Newborn ; Male

OBJECTIVE To investigate the coding genes of ?-lactamases,aminoglycoside modifying enzymes and the drug-resistant to chlorhexidine-sulfanilamide genes on 20 Acinetobacter baumannii isolates in Xinjiang.METHODS Twenty strains of A.baumannii were isolated from hospitalized patients,and 9 kinds of ?-lactamases genes,3 kinds of aminoglycoside modifying enzymes genes and drug-resistant to chlorhexidine-sulfanilamide genes were detected.The drug-resistant to chlorhexidine-sulfanilamide genes were labeled and cluster analysis was performed to analyze the affinity of strain.RESULTS The detection rates of ?-lactamases coding genes of TEM,ADC and SHV groups were 65%,60% and 5%,respectively.The others were not found in all 20 isolates tested.The detection rates of aminoglycoside modifying enzymes coding genes of aac(3)-Ⅰ,aac(6′)-Ⅰ and aac(3″)-Ⅰwere 60%,65%and 70%,respectively.And the detection rates of qacE△1-stull genes were 70%.There were 9 strains showed clone transmission according to cluster analysis.CONCLUSIONS Drug-resistance of the 20 strains to ?-lactam and aminoglycosides is connected with ?-lactamases and aminoglycoside modifying enzymes,and there exists clone transmission.

CD36 Antigens ; metabolism ; Cell Aggregation ; Cholesterol ; blood ; Foam Cells ; pathology ; Glomerulonephritis, IGA ; blood ; metabolism ; pathology ; Glomerulonephritis, Membranoproliferative ; blood ; metabolism ; pathology ; Glomerulonephritis, Membranous ; blood ; metabolism ; pathology ; Glomerulosclerosis, Focal Segmental ; blood ; metabolism ; pathology ; Humans ; Nephritis ; blood ; metabolism ; pathology ; Nephritis, Hereditary ; blood ; metabolism ; pathology

·AIM: To analyze the clinical manifestation of Alport syndrome, especially the ocular features.·METHODS: The physical, ophthalmologic and audiologic examination results of thirty two patients with Alport syndrome were analyzed retrospectively.·RESULTS: Thirty (93.7%) patients had some family history. All patients had renal disease: eighteen(56.3%) patients with chronic renal failure, four(12.5%) patients with renal insufficiency, and the other ten(31.3%) patients with hematuria. Twenty (62.5%) patients had sensorineural deafness. Thirteen (40.6%) patients had ocular deformity, five(15.6%) patients had typical ocular changes: three patients with anterior lenticonus, and two patients with macular flecks.·CONCLUSION: Ocular anomalies are not requisite for the diagnosis of Alport syndrome. But its typical ocular features should be recognized by the ophthalmologists which supports the diagnosis.

OBJECTIVESTo probe into the relationship between the CT grouping of liver cirrhosis and its complications as well as clinical condition.

METHODSOn the basis of CT findings in 357 cases with liver cirrhosis, CT grouping of the cirrhosis was performed. The complications of the cirrhosis, including enlargement of spleen, varicose collateral veins, ascites, pleurorrhea, primary liver carcinoma, gallbladder stone, etc, of all groups were analyzed. According to examination the blood routine, and the serum urea nitrogen (SUN), creatinine and uric acid, the condition of spleen function accentuation and liver-kidney syndrome were estimated.

RESULTSThree hundred and fifty-seven cases with cirrhosis were divided into homogeneous group (87 cases, 24.4%), segmental group (41 cases, 11.5%) and nodal group (229 cases, 64.1%). The grade of spleen enlargement in the segmental group and the nodal group was significantly greater than that in homogeneous group. The cases with varicose in the segmental group was the most (70.7%), in the nodal group next (17.0%) and in the homogeneous group the least (2.3%). There was significant difference among three groups. In the nodal group, there was ascites in 182 cases (79.5%) and significantly more than that in the segmental group (11 cases, 26.8%) and the homogeneous group (9 cases, 10.3%), and the former significantly more than the latter. There were 68 cases (29.7%) with primary liver carcinoma in the nodal group, and significantly more than that in the segmental group (1 case, 2.4%) and the homogeneous group (5 cases, 5.7%). The cases with hemoglobin decrease in the nodal group were significantly more than that in the homogeneous group. The averages of hemoglobin and platelet in the nodal group and the segmental group were significantly lower than that in the homogeneous group. The cases with SUN increase in the nodal group and the segmental group were significantly more than that in the homogeneous group. The concentration of SUN in the nodal group was significantly higher than that in the homogeneous group.

CONCLUSIONSThere are close relationship between the grouping of liver cirrhosis on basis of CT findings and complications of the cirrhosis. The practice of grouping might be useful for estimating clinical condition

Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Kidney ; physiopathology ; Liver Cirrhosis ; complications ; diagnostic imaging ; physiopathology ; Male ; Middle Aged ; Tomography, X-Ray Computed

Objective To establish a rapid and accurate detecting method for sulfite in food residues. Methods Applying an fluorine ionsalkali - stable liquid to extract ultrasonically. After removing the suspended substances by high - speed centrifugation,we used online dialysis - ion chromatography to determine sulfite. Chromatographic conditionswere set as follows:Metrosep A Supp 5 - 150 / 4. 0 anion analysis column,using 3. 2 mmol / L sodium carbonate - 1. 0 mmol / L sodium bicarbonate 5% acetone as the eluent,with 0. 70 mL / min flow rate,20 μL injection volume and a conductivity detector. Results Sulfites( calculated as sulfur dioxide)manifested a good linear relationship when its concentration ranged from 0. 50 μg / mL to 50. 00 μg / mL,r = 0. 999 8. The minimum detection limit was 0. 54 mg / kg. The spiked concentrations were 10. 0 mg / kg,100. 0 mg / kg,500. 0 mg / kg,900. 0 mg / kg to samples. Relative standard deviation(n = 6,% )was 5. 04,1. 20,0. 56,0. 98,with the recovery rate between 85. 4% - 104. 4% . Conclusion This method is simple,rapid,with high sensitivity and accuracy of measurement,and which is suitable for the detection of sulfites content in food matrix.

[Abstract] Objective To study the morphology, muscle architecture index and distribution pattern of intramuscular nerve dense area of elbow muscle, so as to provide anatomical location for poster-lateral approach of elbow joint and transplantation of elbow muscle flap. Methods Through gross anatomy, muscle architecture index and modified Sihler’s intramuscular nerve staining, 10 cases with an average age of 64. 2 years were selected. Results The elbow muscle was approximate triangle, the muscle wet weight was (6. 31±0. 85) g, the muscle length was (6. 24±0. 78) cm, the muscle fiber length was (4. 74±0. 88) cm, pennation angle(70. 60±6. 41)°and the muscle physiological cross-sectional area was (0. 41±0. 15) cm

Objective To develop pathogenic surveillance on measles and to effectively isolate measles virus. To know the genetic characterizations and molecular epidemiology of wildtype measles viruses from 2005 to 2007 in Guangdong Province,and provide the scientific basis for measles control and eradication. Methods Vero/ Slam cell line were used,measles viruses were isolated from throat swabs or urine specimens collected from uspected measles patients in outbreaks and sporadic patienta. A 450 nucleotides fragment of the C-terminal of the nucleoprotoin (N) gene was amplified and by RT-PCR and subjected to scquenee and phylngenetie analysis using Bio-Edit software. Results 82 wild-type measels virus were obtained from 377 throat swabs and urine specimens from 2005 to 2007 in Guangdong Province measles lab. The measles isolation rate was 23.58 % in 2005,17.11% in 2006,39.13% in 2007.The succeed rate of virus isolation is related to the quality of specimens collected and the days after rash occurrence. Conclusions We have grasped the techniealability of measles virus isolation and confirm action,and got higher isolation ratio. The wild-type measles virus isolated from Guangdong Province is of H1 genotype from 2005 to 2007,which is the same as the dominant genotype circulation.

Objective To compare the impact of the first 24 hours mean blood glucose (MBG)level and admission glucose (AG) during hospitalization on the short term mortality and combined end point events in patients with ST-segment elevation acute myocardial infarction (STEMI). Methods A total of 7446 Chinese STEMI patients hospitalized within 12 hours of symptom onset were included. Plasma glucose was measured at admission, 6 and 24 hours after admission, respectively. The MBG level through the first 24 hours for each patient was calculated. Patients were stratified into six groups according to their MBG levels: ＜4.5, 4.5 -5.5, 5.6-7.0, 7.1 -8.5, 8.6-11.0and ＞11.0 mmol/L. The incidence of all cause mortality and combined end point of death, re-infarction, cardiogenic shock, recurrence ischemia, and stroke at 7 days and 30 days post hospitalization were analyzed. Nested models were compared to determine whether logistic regression models that included MBG provided a significantly better fit than logistic regression models included AG. Results Compared with the MBG of 4. 5 - 5.5 mmol/L group, 7-day and 30-day mortality and combined end point events increased in proportion to plasma MBG level increase.Multivariate logistic regression analysis showed that elevated MBG ( equal or greater than 7. 1 - 8. 5 mmol/L) level is an independent predictor of 7-day and 30-day mortality and combined end point events. Nested models analysis showed that the prognostic impact of MBG is superior to AG . P ＜ 0. 001 ) on predicting 7-day and 30-day mortality and combined end point events in this patient cohort. Conclusion Elevated MBG ( ≥7. 1 mmol/L) level is an independent predictor of 7-day and 30-day mortality and combined end point events. MBG is superior to AG on predicting short-term prognosis in this patient cohort.

OBJECTIVETo construct the multi-probe ribonuclease protection assay (RPA) template set to be used for detecting expression patterns of MD-2, TLR4, CD14 mRNAs in human peripheral blood mononuclear cells.

METHODSThe designed cDNA fragments of the three genes were generated by polymerase chain reaction (PCR) using specific primers and directionally cloned into EcoR I and Hind III sites of expression plasmid pSP72 containing the T7 promoter, the linearized plasmids was used as template to synthesize anti-sense RNA probes. Then we extracted total RNA from peripheral blood mononuclear cells (PBMC) and detected the dynamic expression patterns of the three genes with RPA method.

RESULTSThe proper sequence and orientation of the template set were confirmed by sequencing and the template set was successfully used to assay TLR4, MD-2 and CD14 mRNAs in human PBMC. The results showed that the three detected genes decreased transiently 1-3 hours after 100 ng/ml LPS stimulation.

CONCLUSIONSThese new RPA multi-probe set provided valuable tool for the simultaneous quantitative determination of expression of TLR4, CD14 and MD-2 mRNAs in both constitutive and inducible types.

Antigens, Surface ; analysis ; Base Sequence ; Biological Assay ; Cells, Cultured ; DNA ; analysis ; genetics ; Gene Expression Profiling ; methods ; Humans ; Lipopolysaccharide Receptors ; analysis ; Lymphocyte Antigen 96 ; Membrane Glycoproteins ; analysis ; Molecular Probe Techniques ; Molecular Sequence Data ; Monocytes ; metabolism ; RNA Probes ; analysis ; genetics ; Receptors, Cell Surface ; analysis ; Receptors, Immunologic ; analysis ; Ribonucleases ; Toll-Like Receptor 4 ; Toll-Like Receptors