OBJECTIVE:To establish a RP-HPLC method for the determination of nitrochlodipine in rabbit plasma.METH-ODS:Chromatographic conditions and extractive conditions were optimized with orthogonal design,internal standard:felodipine,solid phase extract column:C18,column size:NOVA-PackC18(4.6mm?250mm),detection wavelength:237nm.RESULTS: The optimal chromatographic conditions were:mobile phase acetonitrile-water(55∶45),flow rate 1.0ml/min,column temperature 45℃.The extraction conditions were A1 B1 C3.The calibration curve was linear within the range of 6.25～400?g/L(r=0.9 996) and the minimum limit of detection was 2.5?g/L.The average RSD of within-day ranged from 3.81% to 5.58% and that of day-to-day from 4.60% to 8.76%.The average recovery ranged from 100.7% to 101.8%.CONCLUS-ION:The method is simple,rapid,highly accurate and precise.It can be used for the quantitative determination of nitrochlodipine in plasma.

Objective: Enteral nutrition was used to correct the malnutrition in the patients suffered from esophageal rupture postoperatively. Methods: The naso intestinal tube was placed during operation and the enteral nutrition was used postoperatively. The albumin, prealbumin and transferrin were measured before and day1, 5, 8 and 12 after operation. Results: All 27 patients were discharged with no death. Albumin, prealbumin and transferrin decreased on the 1st day postoperatively and reached the preoperative level on the fifth postoperativeday. Conclusion: Enteral nutrition plays an important role in the postoperative treatment for esophageal rupture.

Objective To evaluate the effect of Rab23 on the proliferation of a squamous cell carcinoma cell line Sa3,and to investigate its mechanisms.Methods Cultured Sa3 cells were classified into four groups:normal control group transfected with green fluorescent protein (GFP),Rab23-overexpressing group transfected with a GFP-labelled Rab23-overexpressing plasmid,Rab23-silencing group transfected with a plasmid carrying a Rab23-targeting shRNA,empty vector group transfected with an empty vector.After additional culture for different durations,plate colony formation assay and flow cytometry were performed to evaluate the proliferative activity of Sa3 cells,and Western blot was conducted to detect the expression of Erl/phosphorylated-Erk in Sa3 cells.Statistical analysis was carried out by t test,one-way analysis of variance and Bonferroni's multiple comparison test.Results Stable Sa3 cell lines with overexpression or silencing of Rab23 were established by plasmid construction and lentivirus-mediated transfection.The plate colony formation assay showed that the colony formation rate was significantly lower in the Rab23-overexpressing group than in the normal control group (2.3％ ± 0.2％ vs.3.6％ ± 0.3％,P ＜ 0.05),but higher in the Rab23-silencing group than in the empty vector group (4.1％ ± 0.2％ vs.1.8％ ± 0.03％,P ＜ 0.01).Rab23 overexpression induced G1 phase arrest in Sa3 cells.The proliferation index was significantly decreased in the Rab23-overexpressing group compared with the normal control group (0.581 ± 0.035 vs.0.698 ± 0.018,P ＜ 0.05),but increased in the Rab23-silencing group compared with the empty vector group (0.567 ± 0.015 vs.0.444 ± 0.014,P ＜ 0.01).As Western blot showed,there were no significant changes in the expression of Erk in the Rab23-silencing or-overexpressing group compared with the normal control group,whereas the expression of p-Erk was attenuated in the Rab23-overexpressing group compared with the normal control group,but enhanced in the Rab23-silencing group compared with the empty vector group.Conclusions Rab23 could inhibit the proliferation of Sa3 cells,which may be associated with the Erk pathway.

Objective: To evaluate the effect of nutritional support for lung transplantation patients.Methods: The lung transplantation patient received perioperative enteral nutrition(EN) .Exogenous glutamine(Gln) and recombinant human growth hormone(rhGH) were postoperatively used for 7-14 days.Results: The patients weight increased from 53 kg to 55 kg. No respiratory failure and acute rejection occurred postoperatively. The patient recovered fluently.Conclusion: Appropriate perioperative nutritional support and postoperative metabolic intervention can facilitate the recovery of lung transplant patient.

Objective To estimate the biological effects of leptin on human HaCaT keratinocytes and explore their molecular mechanisms.Methods Cell counting kit-8 (CCK-8) was used to evaluate the proliferation of cultured HaCaT cells treated with different concentrations of leptin for 24 and 48 hours.Some HaCaT cells were classified into four groups to remain untreated,be treated with leptin (100 μg/L) and piceatannol (a specific inhibitor of STAT3 phosphorylation) alone or in combination for 24 hours,respectively,followed by the evaluation of cell proliferation using CCK-8 kit.Flow cytometry was performed to assess cell cycle of HaCaT cells treated with leptin of 100 μg/L,Western blot to determine the phosphorylation level of Erk1/2 and STAT3 in HaCaT cells treated with leptin of 100 μg/L for different durations.Statistical analysis was done by Student's t-test for unpaired data using GraphPad Prism 5 software.Results The proliferation of HaCaT cells was accelerated to different degrees after treatment with leptin of 50 and 100 μg/L for 24 and 48 hours,and the accelerating effect was in a dose-dependent manner within 24 hours (r =0.9989,P ＜ 0.05).Piceatannol apparently inhibited the promotive effect of leptin on the proliferation of HaCaT cells.There was an obvious elevation in the percentage of cells at S phase ((57.70 ± 5.88)％ vs.(42.50 ± 7.55)％,P ＞ 0.05),but a significant decrease in that at G0/G1 phase ((39.70 ± 1.57)％ vs.(45.20 ± 1.44)％,P ＜ 0.05),with a significant increase in proliferation index (0.603 ±0.0157 vs.0.564 ± 0.0144,P ＜ 0.05) in HaCaT cells treated with leptin of 100 μg/L for 24 hours compared with the untreated controls.Western blot showed that leptin of 100 μg/L markedly enhanced the phosphorylation level of STAT3 in HaCaT cells.Conclusion Leptin may upregulate the proliferation of HaCaT cells through activation of STAT3 pathway.

Objective To explore the correlation of rhinovirus infection and the morbidity of asthma in children.Methods The RV gene in nasopharyngeal secretion of 30 children with asthma acute exacerbation(asthma group),30 children in asthma clinical remission(remission group) and 30 healthy children (control group) were detected by reverse transcription-polymerase chain reaction(RT-PCR).The total serum IgE(T-IgE) was determined by chemiluminescence.The eosinophil(EOS) count (EOS%) of peripheral blood and lung function were also measured.Results The rhinovirus infection rate was 36.67% in asthma group and 3.33% in remission group.No rhinovirus was detected in control group.The rate of rhinovirus infections in asthma group was significantly highter than that in remission group and control group(?2=7.50,13.47 Pa0.05). FEV1% was (62.73?13.54)% in asthma rhinovirus infection and (86.42?17.78)% masthma with no rhinovirus infection.There was remarkable difference between 2 groups(F=14.553 P﹤0.05). The T-IgE was (836.32?44.801) kU/L and EOS% was 10.63?4.09 in asthma group with rhinovirus infection.The total IgE was (439.10?231.28) kU/L and EOS% was 5.04?2.64 in asthma group with no rhinovirus infection.There was significant difference between 2 groups(U=2.475,F=20.806 Pa0.05).Conclusions There is a close correlation between rhinovirus infection and acute attack of asthma(or worsening asthma) in children. Asthma exacerbations would be likely to happened easier because of rhinovirus infection in asthmatic children with high T-IgE.

AIM: To determine the effect of suction duration on thickness and diameter of corneal flap created by microkeratome in porcine eyes in laser in situ kerato-mileusis (LASIK).METHODS: Sixty porcine eyes were randomly assigned to three groups according to different suction durations: group 1 (10 seconds), group 2 (20 seconds), and group 3 (30 seconds). A Moria M2 microkeratome (Moria, France) with a 160μm head was used to create a corneal flap. Corneal flap thickness was measured by automated ultrasonic pachymetry, and the flap diameter was measured by a vernier caliper.RESULTS: The flap thickness of group 1, group 2 and group 3 was 146.05±13.46μm, 157.35±18.95μm and 169.25±21.02μm, respectively. There was a statistically significant difference among three groups (P=0.001). The mean flap diameter in groups 1, 2 and 3 was 8.63±0.19mm, 8.89±0.24mm and 9.06±0.18mm, respectively. A statisti-cally significant difference was found among groups (P<0.01).CONCLUSION: In LASIK in porcine eyes, an increase in suction duration resulted in a thicker and greater flap.

Objective To discuss the national standard method of"General Test Method in Salt IndustryDetermination of Iodine Ion"and point OUt an incorrect concentration of sodium hypochlorite(NaClO)solution which is not matched with the concentration of oxalic acid solution being used in the national standard method.Methods The iodine ion in the reference salt was determined step by step as stipulated in the method.During the test,the concentration of the NaClO solution was altered from 5.0%to 0.1%in order to screen the suitable ranges of the concentration of NaClO solution.Results 5.0%NaClO solution was used according to the national standard method,which led to a significant deviation up to 700 ms/kg.The relative errors of the standard iodized salt determination were respectively 2312.0%,185.0%,4.0%,3.3%,-0.6%,-3.0%,-3.3%with different concentration NaClO(5.0%,3.0%,2.5%,1.0%,0.3%,0.2%,0.1%).Conclusion Under the circumstance that the concentration of the oxalic acid solution remains the same.the concentration of NaClO solution must be revised into 0.3%～2.5%in the national standard method to decrease testing errors.

OBJECTIVE To assess the method and reliability of the platysma myocutaneous flap with posterior based pedicle for defects in the region of parotid gland and masseter muscle.METHODS The clinical data of 6 patients who were reconstructed with platysma myocutaneous flap with posterior based pedicle for defects in the region of parotid gland and masseter muscle from 2006 to2007 were studied. The blood supply and design of the flap,the keys in operation and the outcome after operation were also discussed.RESULTS All the patients were followed up from 2 months to one year.The color of the platysma myocutaneous flap was matched with the facial skin.All the operation results were satisfied. CONCLUSION Platysma myocutaneous flap has abundant blood supply.The operation is easy and reliable.It is suitable for reconstruction of the defects in parotid gland and masseter muscle region.