Burns, Inhalation ; complications ; Humans ; Male ; Middle Aged ; Nitric Acid ; adverse effects ; Pulmonary Edema ; chemically induced ; therapy

Objective To investigate the effects of verapamil on hyperalgesia and spinal cord dorsal horn c fos expression after trauma in rats Methods Forty Wistar rats weighing 160 220g were randomly divided into five groups with eight animals in each group Traumatic pain model was made by amputation of right hind limb 0 5cm below ankle joint In group A animals received intraperitoneal verapamil 10mg/kg after trauma; in group B fentanyl 30 ?g/kg; in group C verapamil 10mg/kg plus fentanyl 30?g/kg; in group D normal saline; in group E animals received neither trauma nor verapamil or fentanyl The pain threshold of the skin surrounding the site of trauma was measured at 15 min, 30 min,1h and 2h after trauma and the interval between the radiation heat applied to the skin over ankle and the withdrawal of the hind limb was taken as pain threshold Fos like immunoreactive (FLI) neurons were determined in 1 2 luminal of ipsilateral lumber spinal cord dorsal horn with FOS immunohistochemistry technique 2h after trauma Results The pain threshold of the skin surrounding trauma site decreased significantly at 15 min, 30 min, 1h and 2h after trauma (P

Zymonomas mobilis was transformed with a polyhydroxybutyrate synthesis operon phbCAB equipped with a pdc promoter from Z. mobilis. For the first time,PHB was produced in recombinant Z.mobilis. Shake flask studies indicated that accumulation of PHB in Zymomonas mobilis increased approximately 10% ethanol productivity for the first 48h of anaerobic fermentation. After that,the PHB effect was observed as insignificant probably due to the exhaustion of the sugar.

Animals ; Colonoscopy ; methods ; Colorectal Neoplasms ; pathology ; Disease Models, Animal ; Heterogeneous-Nuclear Ribonucleoproteins ; metabolism ; Precancerous Conditions ; pathology ; Rodentia

Adolescent ; Cranial Nerve Neoplasms ; Facial Nerve ; Humans ; Male ; Neurilemmoma ; Parotid Region ; pathology

AIM:To investigate the effect of case-based learning ( CBL) teaching combination with paper review method in the teaching of ophthalmology residents. ·METHODS:The study was conducted from 2015 to 2016. The residents in Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital were included in the research. During the year of 2015, the traditional lecture-based learning ( LBL) method was applied (as a control group). During the year of 2016, the CBL teaching combination with paper review teaching method was applied (as an experimental group). At the end of the course, exams and questionnaires were conducted to evaluate the teaching effect of two different methods. The exams consisted of theoretical and operational assessment. The teaching satisfactions, learning interests, scientific research interests and clinical abilities were included in the questionnaires. The data was analyzed by SPSS 16. 0. ·RESULTS:The results of final exams indicated that the scores of the experimental group (88. 2±6. 5) were higher than the control group ( 75. 6 ± 6. 0 ). The difference showed statistically significant (t=6. 68, P<0. 05). The results of questionnaires indicated that students showed much more satisfied with CBL teaching combination with paper review teaching method ( 91%) than traditional teaching method (50%, x2 =8. 84, P<0. 05). Students in the experimental group improved learning interest ( x2 =6. 29, P<0. 05), increased research interest (x2=4. 54, P<0.05) and approved clinical ability (x2 =4. 25, P<0. 05). The comparison of two groups showed the statistically difference (P<0. 05). ·CONCLUSION: CBL teaching combination with paper review teaching method is beneficial to improve the teaching effect, and it is also beneficial to train residents'clinical skills and research abilities.

Now symptomatic extracranial and intracranial stenting usually uses bare metal stents, but the incidence of restenosis is higher.The studies of drug-eluting stems for the treatment of coronary atherosclerosis have suggested that the incidence of restenosis decreases significantly. Although extracranial and intracranial drug-eluting stem placement appears to be safe,further large,prospective,randomized,controlled trials are needed to demonstrate its safety and effectiveness.

OBJECTIVE To assess the method and reliability of the platysma myocutaneous flap with posterior based pedicle for defects in the region of parotid gland and masseter muscle.METHODS The clinical data of 6 patients who were reconstructed with platysma myocutaneous flap with posterior based pedicle for defects in the region of parotid gland and masseter muscle from 2006 to2007 were studied. The blood supply and design of the flap,the keys in operation and the outcome after operation were also discussed.RESULTS All the patients were followed up from 2 months to one year.The color of the platysma myocutaneous flap was matched with the facial skin.All the operation results were satisfied. CONCLUSION Platysma myocutaneous flap has abundant blood supply.The operation is easy and reliable.It is suitable for reconstruction of the defects in parotid gland and masseter muscle region.

Objective To investigate the effects of tagged fluid and substraction in CT virtual colonoscopy (CTVC) on the conspicuity of polyps and to confirm the optimal attenuation value and the optimal viewing window. Methods Polyps measured 3-10 mm were created in fresh porcine colon in vitro and submerged in saline and mixed with positive contrast medium for the CT values of 200 HU, 400 Hu, 600 HU, and 800 HU. Polyps were measured before and after substraction and compared with those in the control group. The effects of different viewing windows and different attenuation values on the measurement and conspicuity of polyps were analyzed. Results The optimal attenuation value of tagged fluid was 800 HU and the optimal viewing window was colon (-150 HU, 1 500 HU) and bone (500 HU, 2 500 HU). Conclusion The combination of tagged fluid and CT substraction can improve the conspicuity of small polyps covered by colonic fluid.

Objective To develop a visible detection system prepared by protein microarrays which was based on GoldMag particles-labeled technique and compare the results of detection using the protein which was labeled with GoldMag particles and colloidal gold.Methods Human IgG was printed on the glass slides modified with epoxy groups,and goat anti-human IgG conjugated with GoldMag particles and colloidal gold respectlively was then added on the slide.The glass slides were incubated,and then the black images of microarray spots were produced by immuno-gold-silver staining method and observed by naked eyes and recorded with common flatbed scanner.Results The high signal-to-noise ratio could be obtained when the optimized procedures of GoldMag particles-labeling probe were introduced to the protein chip.The conditions of optimum assay were as follows:the spotting concentration of human IgG was 0.2 mg/ml,the glass slides were incubated at 37 ℃ for 2 hour to immobilize human IgG,and the silver enhancement time was 10 min-15 min.Parallelly,the optimized conditions for colloidal gold were as follows:the spotting concentration of human IgG was 0.1 mg/ml,the slides were incubated at 37 ℃ for 1 hour to immobilize human IgG,and the silver enhancement time was 15 min-20 min.Conclusions GoldMag particles-labeled protein technique is comparable to colloidal gold in applying to protein microarrays.The method is considerably simple and practical,and the protein labeled by GoldMag particles could be quantitative.