2.Application of Functional Magnetic Resonance Imaging in Aphasia(review)
Lu-ping SONG ; Jian-min XU ; Tong ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(11):926-928
Functional magnetic resonance imaging(fMRI),as a noninvasive technology of measuring human brain function,has been used in neurolingustic research on normal subjects and on patients with brain damage,to investigate the neural basis underlying language processing.Such studies complement and development the classical knowledge accumulated on aphasia,provide new insights into the neural mechanisms of aphasia and the plasticity of recovery from aphasia,and might be helpful to evaluate the capability of language functional recovery and the efficiency of rehabilitative strategies also.
4.Molecular authentication of Sailonggu and its resource distribution in Qinghai-Tibet Plateau.
Fang ZHAO ; Xiao-gong DENG ; Tong-zuo ZHANG ; Jian-ping SU ; Gong-hua LIN
China Journal of Chinese Materia Medica 2015;40(3):399-403
To provide accurate information on geographic distribution of crude drug Sailonggu in the plateau, we identified zokor species (Eospalax spp.) in Qinghai-Tibet Plateau using molecular methods. Based on the mitochondrial cytochrome B (cytb) gene sequences, we then extracted haplotypes from these sequences and reconstructed phylogenetic trees for the haplotypes using both maximum likelihood (ML) and Bayesian inference (BI) methods. Based on the trees, the species of each sample were determined. Five hundred and three samples from 35 populations were sequenced and their whole cytb sequences (1140 bp) were obtained. From these sequences 150 haplotypes were detected, in which, 126 were Eospalax baileyi, 20 were E. cansus, and 4 were E. smithi of the 35 populations, 28 were E. baileyi type, 5 were E. cansus type, and the remaining 2 were mixed of E. baileyi + E. cansus (DT2) and E. baileyi + E. smithi (ZN3). The results showed that, the regions around the Qinghai lake and near the upper stream of Yellow River started at Guide could be viewed as the producing area of authentic Sailonggu, and also, the cytb gene is a powerful molecular marker to determine the species of zokors as well as for the authentication of geographic distribution of Sailonggu.
Animals
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Bone and Bones
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metabolism
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Haplotypes
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Medicine, Tibetan Traditional
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Phylogeny
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Rodentia
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classification
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genetics
5.The Study of Treatment of Odor Gas Emitted from the Wastewater Plant by the Biofilter
Jian-Jun LI ; Yan-Zhen LIANG ; Tong-Sheng CHEN ; Ying-Hua CEN ; Guo-Ping SUN ;
Microbiology 1992;0(05):-
A pilot-scale research on purification of odorous gas emitted from wastewater treatment plant using a biofilter was conducted. The aim of this study is to check on the performance of biofilter running in various conditions and the effect of pH fluctuations on the performance of biofilter. The relation between distribution of microorganism and removal of odorous gases were also discussed here. The experimental results show that the predominant odor-causing gas can be efficiently eliminated by a biofilter inoculated with deodoring microorganism which were isolated previously. Moreover the biofilter had been proved having good tolerance to shocking loads of pollutant and can operate well in the condition of low pH.
6.Expression of mammary serine proteinase inhibitor in oral squamous cell carcinoma and its clinical significance.
Jun LUO ; Lan ZHOU ; Gen-jian ZHENG ; Tong JI ; Lai-ping ZHONG
Chinese Journal of Stomatology 2009;44(10):606-610
OBJECTIVETo investigate the expression of mammary serine proteinase inhibitor(Maspin) in patients with oral squamous cell carcinoma and its relationship with clinicopathological features.
METHODSThe Maspin protein expression was examined in 45 patients with oral squamous cell carcinoma and in non-malignant oral epithelia by immunohistochemical staining. The relationship between the Maspin protein expression and the clinicopathological parameters was statistically analyzed.
RESULTSThe Maspin protein expression in the cancerous tissues of oral squamous cell carcinoma was lower than that in the non-malignant oral epithelia (P = 0.001). Negative correlation was found between the Maspin protein expression in cancerous tissue and the status of lymph node metastasis (P = 0.038). Positive Maspin protein expression indicated negative lymph node metastasis, and negative correlation was also found between the Maspin protein expression and the postoperative metastasis (P = 0.004). Positive correlation was found between the Maspin expression and the patients' survival rate (P = 0.014), patients with positive Maspin protein expression having higher survival rate than those with negative Maspin expression.
CONCLUSIONSMaspin might be useful as a potential prognostic marker for oral squamous cell carcinoma.
Adult ; Aged ; Aged, 80 and over ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Female ; Humans ; Male ; Middle Aged ; Mouth Neoplasms ; metabolism ; pathology ; Serine Proteinase Inhibitors ; metabolism ; Serpins ; metabolism
7.Preventive effect of genistein on malignant transformation of oral leukoplakia.
Ya YANG ; Zeng-tong ZHOU ; Jian-ping GE
Chinese Journal of Stomatology 2006;41(2):116-117
OBJECTIVETo investigate the preventive effect of genistein on the malignant transformation of oral leukoplakia in hamster cheek pouch.
METHODSSixty male Syrian golden hamsters were randomly divided into 4 groups, with two of 20 and two of 10. DMBA solution (0.5% in acetone) was applied topically to the left cheek pouch of male Syrian golden hamsters in group A and B, while animals in group C were painted with acetone, 3 times a week for 6 weeks. For the next 12 weeks, animals in group B received genistein (10 mg.kg(-1).d(-1)) daily by gavage; animals in group A and C received same volume of saline; animals in group D serving as blank control. At the end of the experiment, animals were sacrificed and tissue samples were collected for histopathological examination.
RESULTSNo significant differences in the tumor latency and tumor incidence were observed between the control and genistein treated group.
CONCLUSIONSNo inhibitory effect of genistein on the transformation of oral leukoplakia was observed in hamster.
Animals ; Cricetinae ; Genistein ; pharmacology ; therapeutic use ; Leukoplakia, Oral ; pathology ; prevention & control ; Male ; Mesocricetus ; Mouth Mucosa ; drug effects ; pathology ; Precancerous Conditions ; pathology ; prevention & control
8.Study on isoflavone active aglycone preparation by immobilized beta-glucosidase from Aspergillus niger.
Li-Hua PAN ; Jian-Ping LUO ; Shao-Tong JIANG
Chinese Journal of Biotechnology 2007;23(6):1060-1064
With sodium alginate as a carrier and glutaraldehyde as the crosslinking agent, an improved immobilization method of beta-glucosidase for production of soybean genistein was developed. As compared with entrapment or entrapment-crosslinkage, crosslinkage-entrapment that beta-glucosidase was treated with glutaraldehyde and then entrapped in sodium alginate remained high loading efficiency and activity recovery, Effects of bead sizes, concentrations of alginate and glutaraldehyde as well, on the loading efficiency and activity recovery were assessed. When compared with the free enzyme, the optimum temperature, pH value and Km of the immobilized beta-glucosidase were respectively shifted from 50 degrees C to 40 degrees C, 4.5 to 4.0 and 2.57 microg/mL to 2.02 miocrog/mL. The stabilities of the immobilized beta-glucosidase were considerably better than that of the native enzyme. The immobilized beta-glucosidase was employed to genistein production, 84.94% of the activity and 56.04% of conversion were kept after consecutive use of 6 times.
Alginates
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chemistry
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Aspergillus niger
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enzymology
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genetics
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Enzyme Stability
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Enzymes, Immobilized
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metabolism
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Genistein
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chemical synthesis
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chemistry
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Glucuronic Acid
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chemistry
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Glutaral
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chemistry
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Hexuronic Acids
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chemistry
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Isoflavones
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chemistry
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Soybeans
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chemistry
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beta-Glucosidase
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chemistry
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metabolism
9.Research on application of determination of MMP-13 in osteoarthritis.
Wen-Xiao CHEN ; Fang-Jun SHAN ; Hong-Ting JIN ; Ping-Er WANG ; Lu-Wei XIAO ; Pei-Jian TONG
China Journal of Orthopaedics and Traumatology 2014;27(7):617-620
Osteoarthritis (OA) is a complex chronic progressive disease attacked by biological and mechanical factors and a result from the anabolic and catabolic imbalance in chondrocyte, subchondral bone and extracellular matrix(ECM). Etiology and pathological of OA are not yet entirely clear. The degradation and destruction of collagen II caused by matrix metalloproteinase -13 (MMP-13) is considered the core factor in the occurrence and development of OA. The research of MMP-13 inhibitor provide ideas and methods for the treatment of OA. In this article,the role and determination of MMP-13 in OA and the development prospect of MMP-13 inhibitor in the treatment of OA research progress were reviewed.
Animals
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Collagen
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metabolism
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Humans
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Matrix Metalloproteinase 13
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analysis
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physiology
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Matrix Metalloproteinase Inhibitors
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therapeutic use
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Osteoarthritis
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drug therapy
;
etiology
10.Effect of spermine on cell growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense.
Ming WEI ; Shao-Tong JIANG ; Jian-Ping LUO
Chinese Journal of Biotechnology 2007;23(2):327-331
The effect of outer spermine on cell growth, accumulation of polysaccharides and utilization of nutrient together with the intracellular polyamine contents were investigated in suspension cultures of protocorm-like bodies from Dendrobium huoshanense. The results indicated that spermine at 0.6 mmol/L was the most effective in increasing cell growth and polysaccharide synthesis. The specific growth rate of cell increased from 0.046d(-1) to 0.054d(-1), and the maximum dry weight and polysaccharide production reached 32.4g DW/L and 2.46g/L respectively, which were 1.32-fold and 1.31-fold that of the control on day 30. The titres of intracellular free polyamines were higher in the cultures treated with spermine than that of the control. Invertase and nitrate reductase activities were found to increase significantly in the cultured cells treated with spermine, which was beneficial to the utilization of carbon and nitrogen source.
Biomass
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Carbon
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metabolism
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Cell Proliferation
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drug effects
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Cells, Cultured
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Dendrobium
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cytology
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drug effects
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metabolism
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Nitrate Reductase
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metabolism
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Nitrogen
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metabolism
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Plant Proteins
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metabolism
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Plant Stems
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cytology
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drug effects
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metabolism
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Polyamines
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metabolism
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Polysaccharides
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biosynthesis
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Spermine
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pharmacology
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Time Factors
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beta-Fructofuranosidase
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metabolism