2.Indoleamine 2,3-dioxygenase and forkhead boxP3 on immune tolerance of gallbladder carcinoma cells
Jian GAO ; Xingyuan JIAO ; Peng ZHANG ; Jun DU ; Jingsen SHI
Chinese Journal of Hepatobiliary Surgery 2012;18(4):252-255
Objective To investigate the expressions of indoleamine 2,3-dioxygenase (IDO) and transcription factor forkhead box P3(FOXP3)in gallbladder adenocarcinoma; and to evaluate the relationship between the expressions of IDO and FOXP3 protein,and clinicopathology and lymph node metastasis of gallbladder carcinoma.Methods The expressions of IDO and FOXP3 in 51 cases of primary gallbladder cancer,90 cases of chronic cholecystitis,and 20 cases of normal gallbladder tissues were studied by immunohistochemical staining. Results The positive expression rates of IDO and FOXP3 in gallbladder carcinoma were 72.5% (37/51) and 60.8% (31/51),in normal gallbladder mucosa were 5% (1/20) and 20.0% (4/20),in cholecystitis and gallstone were 11.1% (10/90) and 32.2% (29/90),respectively.There were significant differences among the three groups in IDO and FOXP3 expressions (P<0.01).The positive expression rates of IDO and FOXP3 were 7.1% and 14.3% for simple hyperplasia,17.7% and 35.3% for atypical hyperplasia,40% and 35% for gallbladder carcinoma (stages Ⅰ-Ⅲ),and 77.4% and 64.5% for gallbladder carcinoma (stages Ⅳ-Ⅴ ).There were significant differences among the four groups in IDO and FOXP3 expressions (P<0.01).There were significant differences among the Nevin stage groups,lymph node metastasis group and gallbladder stone in IDO and FOXP3 expressions.However,there were no significant differences among the sex groups and the age groups in IDO and FOXP3 expressions (P>0.05).In gallbladder carcinoma,the expression of IDO had a positive correlation with FOXP3 expression in lymphocyte (γ=0.406,P=0.003).Conclusion In gallbladder cancer,a high-expression of IDO and an expression of FOXP3 in lymphocyte are closely related with immune tolerance of gallbladder carcinoma.The results provide a reference for clinical use of immunotherapy in gallbladder cancer.
3.CYP2C9*3 and MSA2756G gene polymorphisms in patients with hyperlipemia in Ningxia Hui population
Juyi LI ; Jing JIN ; Peng GAO ; Juan DU ; Jian WANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2004;0(05):-
Objective To investigate the distribution of CYP2C9*3 and methionine synthetase(MSA2756G) genes related to drug therapy in hyperlipidemia patients of Ningxia region as well as its relation with hyperlipidemia.Methods Genotype was determined by using amplication-created restriction sites(ACRS) and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in hyperlipidemia patients.Results Among the 180 hyperlipidemia patients of Ningxia Hui population,the frequency of CYP2C9*3 alleles was 3.33% and mutation rate in men(3.05%) was significantly higher than that in women(0.28%)(P0.05).The frequency of MSA2756G(15.83%) alleles was significantly higher than that in healthy control group(10.25%)(P
4.Impact of down-regulation of histone methyltransferase enhancer of zeste homolog 2 on RUNX3 expressions, proliferation and apoptosis in human pancreatic cancer
Jian DU ; Guang TAN ; Haifeng LUO ; Peng GONG ; Zhongyu WANG
Chinese Journal of Hepatobiliary Surgery 2016;22(4):249-252
Objective To investigate the impact of down-regulation of histone methyltransferase enhancer of zeste homolog 2 (EZH2) on RUNX3 expressions,proliferation and apoptosis in human pancreatic cancer.Methods The expressions of EZH2 and RUNX3 in 38 pancreatic cancer patients and human pancreatic cancer AsPC-1,PANC-1 and BxPC-3 cells were detected by immunohistochemistry and western blot,respectively.Cells were transfected with siEZH2 by lipofectamin 2000.Real time-PCR and western blot were used to detect EZH2 and RUNX3 expressions.Cell growth and apoptosis in vitro and vivo were assessed by MTT,flow cytometry and nude mice experiments,respectively.The correlation among the expressions of EZH2,clinical pathological features and overall survival rate were analyzed.Results Elevated EZH2 and decreased RUNX3 expressions were observed in human pancreatic cancer tissues and cells (P < 0.05).Knockdown of EZH2 reduced cell growth and induced apoptosis in vitro and vivo by upregulating RUNX3 protein expression (P < 0.05).In addition,the EZH2 expressions were correlated with tumor stage,lymph node metastasis and poor prognosis (P < 0.05).Conclusions EZH2 expressions were correlated with malignancy and poor prognosis in pancreatic cancer.Tumor cell proliferation was promoted by EZH2 through down-regulation of RUNX3.EZH2 may be a potential therapeutic target for pancreatic cancer.
5.Single point and two-step acupuncture treatments for shoulder periarthritis.
Wen-Zhong DU ; Yong-Gang HE ; Jian-Peng ZHANG
Chinese Acupuncture & Moxibustion 2011;31(8):720-720
Acupuncture Therapy
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Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Periarthritis
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physiopathology
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therapy
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Shoulder Joint
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injuries
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physiopathology
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Young Adult
6.Long snake moxibustion at Governor Vessel in dog-days for 58 cases of ankylosing spondylitis.
Wen-Zhong DU ; Jian-Peng ZHANG ; Yong-Gang HE
Chinese Acupuncture & Moxibustion 2011;31(10):951-952
Adolescent
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Adult
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Female
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Humans
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Male
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Meridians
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Middle Aged
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Moxibustion
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Spondylitis, Ankylosing
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therapy
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Young Adult
7.Effect of Kangquan Recipe on apoptosis regulatory genes bax and bcl-2 mRNA in prostate of rats.
Yuan-peng HUANG ; Jian DU ; Zhen-feng HONG
Chinese Journal of Integrated Traditional and Western Medicine 2007;27(8):711-714
OBJECTIVETo investigate the effect of Kangquan Recipe (KQR) on apoptosis regulatory genes bax and bcl-2 mRNA in prostate of rats.
METHODSBenign prostatic hyperplasia model rat was established by injecting testosterone after castration. The model rats were killed and prostate glands were removed for examination after being treated with administration of KQR by gastrogavage for 30 days. The wet weight of prostate was measured and the mRNA expressions of bax and bcl-2 in rats' tissue of abdominal lobe of prostate were determined by RT-PCR.
RESULTSCompared with the model group, wet weight of prostate was lower significantly in the groups treated with different dosages of KQR (P < 0.05 or P < 0.01), and that in the high dose KQR treated group was similar to that in the normal group (P > 0.05). Compared with the model group, the expressions of bax mRNA and ratios of bax/bcl-2 were significantly higher and the expressions of bcl-2 mRNA significantly lower in the KQR treated groups (P < 0.01), and these indexes in the high dose KQR treated group were insignificantly different from those in the normal group (P > 0.05).
CONCLUSIONKQR shows an obvious treatment effect on rats with benign prostatic hyperplasia, the mechanism might be through effectively regulating the expressions of bax mRNA and bcl-2 mRNA in prostatic tissue to accelerate the cell apoptosis of prostate in obvious dose-effect manner.
Animals ; Apoptosis ; drug effects ; Drugs, Chinese Herbal ; therapeutic use ; Gene Expression ; drug effects ; Male ; Phytotherapy ; Prostate ; drug effects ; metabolism ; pathology ; Prostatic Hyperplasia ; chemically induced ; drug therapy ; genetics ; Proto-Oncogene Proteins c-bcl-2 ; genetics ; RNA, Messenger ; biosynthesis ; genetics ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Testosterone ; bcl-2-Associated X Protein ; genetics
8.Constitution of a Real-time Monitoring System of Cerebral Hemorrhage with Magnetic Induction.
Bin PENG ; Qinghua TANG ; Jian SUN ; Mingxin QIN ; Wencai PAN ; Zhenwei DU ; Zhao ZHANG ; Shangbin LI
Journal of Biomedical Engineering 2015;32(2):440-445
The real-time monitoring of cerebral hemorrhage can reduce its disability and fatality rates greatly. On the basis of magnetic induction phase shift, we in this study used filter and amplifier hardware module, NI-PXI data-acquisition system and LabVIEW software to set up an experiment system. We used Band-pass sample method and correlation phase demodulation algorithm in the system. In order to test and evaluate the performance of the system, we carried out saline simulation experiments of brain hemorrhage. We also carried out rabbit cerebral hemorrhage experiments. The results of both saline simulation and animal experiments suggested that our monitoring system had a high phase detection precision, and it needed only about 0.030 4s to finish a single phase shift measurement, and the change of phase shift was directly proportional to the volume of saline or blood. The experimental results were consistent with theory. As a result, this system has the ability of real-time monitoring the progression of cerebral hemorrhage precisely, with many distinguished features, such as low cost, high phase detection precision, high sensitivity of response so that it has showed a good application prospect.
Algorithms
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Animals
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Cerebral Hemorrhage
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diagnosis
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Computer Systems
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Magnetics
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Rabbits
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Software
9.The study of relationship between lung function and IFN-γ, IL-4 levels in peripheral blood in patients with pulmonary tuberculosis
Dan LI ; Debing DU ; Jian HE ; Ping YU ; Peng LIU ; Shizhen LUO ; Dongyun CHEN
Chinese Journal of Primary Medicine and Pharmacy 2011;18(3):301-302
Objective To explore the correlation of lung function and IFN-γ and IL-4 levels in peripheral blood in pulmonary tuberculosis patients. Methods The IFN-γ and IL-4 levels in peripheral blood of 75 pulmonary tuberculosis patients(severe 25 cases,moderate 25 casea,and mild 25 cases) and 30 healthy volunteers were measured by ELISA. Meanwhile FEV1 ,FEV1% and MMEF% of lung function in 75 pulmonary tuberculosis patients were measured.Results The levels of IFN-γ[(0. 204 ±0. 018) μg/L] and IL-4[(0. 523 ±0. 035) μg/L] in peripheral blood were significantly different both between tuberculosis group and control group and among tuberculosis patients ( severe,moderate,and mild) (t =7. 685,6. 374 ,all P <0. 05) ;FEV1 ,FEV1% ,and MMEF% of severe and moderate tuberculosis patients were significantly lower than those of mild tuberculosis patients and normal reference value; In tuberculosis patients, FEV1% and MMEF% were negatively related with IL-4 level in peripheral blood( r = -0. 46, -0. 43, all P < 0. 05 ), also significantly positively related with the IFN-γ level in peripheral blood ( r = 0. 47,0. 45, all P <0. 05). Conclusion Pulmonary tuberculosis morbility may be due to cellular levels of patients. The IFN-γ and IL-4 levels in peripheral blood could affect the lung function of pulmonary tuberculosis patients.
10.Effect of HbA1c meeting the standard or not on microalbuminuria,blood lipids and liver enzymes in patients with type 2 diabetes
Keying ZHANG ; Peng CAO ; Jian DU ; Yinan YU ; Yun LI ; Hongfei LUO
Journal of Chinese Physician 2011;13(2):167-169
Objective To investigate the effect of HbA1c meeting the standard or not on microalbuminuria,blood lipids and liver enzymes in patients with type 2 diabetes.Methods A retrospective analysis was performed on 457 subjects who had type 2 diabetes.They were divided into substandard group and standard group according to HbA1c result.The general information and relevant laboratory indicators of patients were.collected and compared between two groups.Results The microalbuminuria,serum triglyceride and liver enzymes (glutamyl transpeptidase,alkaline phosphatase,aspertate aminotransferase) were significantly different between two groups [ (189.8 ± 235.3) mg/dl vs (38.9 ± 85.5) mg/dl,(2.64 ± 2.99) mmol/L vs (2.02 ± 1.50)mmol/L,(41.7 ±52.9)U/L vs (29.7 ±24.9)U/L,(83.6 ±28.6) U/L vs (74.3 ±25.8)U/L,(26.7 ±19.1)U/L vs (22.0 ±10.5) U/L,P <0.05].HbA1c level was positively correlated with microalbuminuria,glutamyl transpeptidase and alkaline phosphatase (r =0.209,0.115,0.11,P <0.01).The microalbuminuria was an independent risk factor of affecting HbA1c to reach the standard (OR = 1.009,P <0.05).Conclusions HbA1c meeting the standard or not can influence many factors except blood glucose.