Air ; analysis ; Air Pollutants, Occupational ; analysis ; Electrophoresis, Capillary ; Esters ; analysis ; Phthalic Acids ; analysis ; Sensitivity and Specificity

ObjectiveTo investigate effects of 8Hz 90dB and 8Hz 130dB infrasound on hippocampi ultrastructure of SD rats.Methods60 male SD rats were randomly divided into the control group and experimental group. Rats of experimental group were exposed to 8Hz 90dB or 8Hz 130dB infrasound 2 hours every day for 1,7,14, 21,28,35 and 42 days. As soon as the exposure finished,the right hippocampus of rat was taken and examined by electron microscopy.ResultsHippocampus ultrastructure of rats was changed after only one day exposure to infrasound and the longer exposure to infrasound, the worse ultrastructure changed. But during the latter period,damages of hippocampus ultrastructure became lighter. Different rules were observed between 90dB and 130dB groups.ConclusionInfrasound of 8Hz 90dB and 8Hz 130dB can make hippocampus ultrastructure of rats damaged mainly as denaturalizations. These changes are nonliner with time, and the hippocampus of rats has adaptability to infrasound.

Objective:To evaluate the effect of short-time group psychological guidance on mental quality of college students. Methods:Designed the content of one day's psychological coach.600 college students received psychological coach successively.60 students were randomly chosen as the intervened group,who were treated with one-day group psychological guidance,while 60 students,who never attended the group psychological guidance,were chosen as control.Assessed and compared the mental quality of the intervened group and the control group with the Faith in People Scale (FPS),Personal Evaluation Inventory (PEI),Self-Rating Anxiety Scale(SAS),Self-Rating Depression Scale (SDS) on the day before or 1 and 12 weeks after coach.Results:There were no significant differences of the scale of FPS,PEI,SAS,and SDS between two groups before coach and there were significant differences between two groups after coach.Conclusion:Short-time group guidance is helpful to developing college students' ability in interpersonal contact and self-confidence and improving depression and anxiety.

Objective To explore caregiver burden and coping strategies of primary caregivers of patients with stroke and their correlation. Methods 120 primary caregivers of patient with stroke from four provincial hospitals of Guangzhou were included in this study. The Chinese version of Zarit Burden Interview (ZBI) and Simplified Coping Style Questionnaire (SCSQ) were applied to assess caregiver burden and coping strategies. Results The total score of caregiver burden of the primary caregivers was (39.54 ± 13.88), 86.7%primary caregivers' burden were in the mild and middle level. Positive coping was used more often than the negative coping among primary caregivers. Positive coping was negatively related to the caregiver burden (P ＜0. O1) and negative coping was positively related to the burden (P ＜ 0. 01). Conclusions Care burden were prevalent in the caregivers of patient with several extent and burden was related with coping style closely. Nurses should guide caregivers to take proper coping style and alleviate disease burden.

Objective To explore the relationship between illness perceptions and medication adherence of patients undergoing percutaneous coronary intervention (PCI).Methods The Illness Perception Questionnaire-Revised(IPQ-R) and Medication Adherence Questionnaire were used to investigate 95 patients undergoing PCI.Results (1)Illness perception status of patients undergoing PCI:average score of identity was (4.87 ± 3.50).Subscales of illness representation were listed according to scores from high to low as follows:timeline (acute/chronic) (3.68 ± 1.1 0),personal control (3.59 ± 0.83),treatment control (3.54 ± 0.65),illness coherence (3.02 ± 1.04),consequences (2.79 ± 0.87),emotional representation (2.24 ± 0.86),timeline cyclical (1.92 ± 1.06).In the part of causes,diet or eating habit scored highest (3.35 ± 1.83).(2)The average total score of medication adherence of patients undergoing PCI was (14.52 ± 1.99) and rate of complete adherence was 35.8％.(3) Identity of patients undergoing PCI was positively correlated with medication adherence (r =0.203,P ＜ 0.05).And personal control (r =-0.236,P ＜ 0.05) and emotional representation (r =-0.228,P ＜ 0.05) were negatively correlated with medication adherence.Conclusions Health care workers should improve symptom identity of patients undergoing PCI,emphasize the importance of adherence to medication,and help them relieve negative emotion in time to enhance their medication adherence.

Adult ; Aged ; Betacoronavirus ; Coronavirus Infections ; complications ; therapy ; Critical Care ; Female ; Humans ; Length of Stay ; Male ; Middle Aged ; Pandemics ; Patient Positioning ; Pneumonia, Viral ; complications ; therapy ; Prone Position ; Respiratory Distress Syndrome, Adult ; therapy ; virology ; Respiratory Function Tests ; Retrospective Studies ; Treatment Outcome

We designed specific primers and fluorescence-labeled probes to develop real-time and conventional RT-PCR assays for detection of human coronavirus NL63 or HKU1. Subsequently, experiments were undertaken to assess diagnostic criteria such as specificity, sensitivity and reproducibility. The detection limit of the real-time RT-PCR assays was 10 RNA copies per reaction mixture. No cross-reactivity was observed between RNA samples derived from designed HCoV and other HCoV or human metapneumovirus. A total of 158 nasopharyngeal swab specimens collected from adult patients with acute respiratory tract infection in Beijing were screened for the presence of human coronavirus NL63 and HKU1 by using real-time RT-PCR and conventional RT-PCR method. The fluorescence quantitative RT-PCR method detected six specimens positive for human coronavirus NL63, five specimens positive for human coronavirus HKU1; and conventional RT-PCR method detected three HCoV-NL63 positive and three HCoV-HKU1 positive, respectively. The convention RT-PCR products of positive samples were obtained and sequence analysis confirmed the reliability of the above methods. In summary, the real-time RT-PCR assay for HCoV- NL63 or HKU1 was more sensitive than conventional RT-PCR and with less time (less than 4 hours) for completion. It may be suitable for molecular epidemiological surveillance and clinical diagnosis for human coronavirus NL63 and HKU1.
Coronavirus ; classification ; genetics ; isolation & purification ; Humans ; Nasopharynx ; virology ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; methods ; Sensitivity and Specificity

Objective To describe the social support and self-efficacy in patients with viral and hepatitis cirrhosis and to investigate the relevance of social support and self-efficacy.Methods By convenient sampling,totals of 105 patients were surveyed with social support scale,and chronic disease self-efficacy scale.Results Totals of 130 questionnaires were handed out and 105 effective questionnaires were recovered,with the rate of 80.77％.The score of social support of patients was (41.87 ± 7.47),and 61.0％ patients with moderate level,39.0％ patients with high level.Among them,subjective support score was (24.43 ± 4.08) with high level,objective support score was(10.45 ± 2.39) with low level,social support score was (6.99 ± 2.44)with moderate level.The self-efficacy score of patients was (48.53 ± 7.78).The scores of social support and its dimensions were markedly and positively correlated with self-efficacy and its dimensions and the value of correlation coefficients ranged from 0.601 to 0.835 (P ＜ 0.01).Conclusions The level of social support in patients with viral and hepatitis cirrhosis is moderate or high,with higher subjective social support level and lower objective social support level.In all the chronic diseases,the self-efficacy in the patients is in higher level.The score of social support is markedly and positively correlated with self-efficacy,the higher social support,the stronger self-efficacy.

This paper investigated the envelope protein E1/E2 quasispecies genetic characterization of 4 HCV positive sera (Genotype 1b: 274, 366, 383; Genotype 2a: 283) in China. Nucleotide acid was extracted and glycoprotein E1/E2 (191-764aa) coding genes were obtained by RT-PCR, positive clones were randomly selected for sequencing. The phylogenetic relationships and the homology of nucleotide and amino acid were analyzed based on E1/E2 coding genes, and some vital functional regions of E1/E2 were characterized. A total of 43 sequences (274: 10; 283: 12; 366: 13; 383: 8) were obtained showing high genetic heterogeneity in HVR1 and HVR2 regions, while sequences of the neutralizing epitopes, transmembrane domain I, II and N-terminal ectodomain were comparatively conservative. Single base (C) insertion mutation at nt1279 ( E1 region, aa313), resulting in a mutated E1 coding protein (beginning at aa 313) and interruption at N terminus (aa 398) of HVR1 region of E2, was dominant quasispecies sequence(11/12) found in serum 283 . This is the first report on E1/E2 quasispecies in Chinese HCV patients and this novel pattern of insertion mutation provides important information for further study on HCV pathogenesis and immune evasion.
Amino Acid Sequence ; Base Sequence ; DNA Mutational Analysis ; Hepacivirus ; genetics ; pathogenicity ; Hepatitis C ; virology ; Humans ; Molecular Sequence Data ; Mutagenesis, Insertional ; Viral Envelope Proteins ; chemistry ; genetics

Objective To express the nuclear capsid protein (N protein) and the spike protein (S protein) of HCoV-HKU1, and to develop the corresponding serum assay for antibody detection. Methods The N protein of HCoV-HKU1 was expressed in E. Coli, anti-N antibody assay was established using Western Blotting with turn-based membrane. HCoV-HKU1 S protein was constructed in the eukaryotic expression plasmids, and confirmed by Western Blotting, S antibody assay was established using indirect immunofluorescence assay (IFA). We analyzed anti-S and anti-N antibody among 100 normal adult serum.Results Expression of S and N protein were confirmed;100 normal adult serum were analyzed using the established serological detection assay, in which HCoV-HKU1 S antibody positive rate was 47% , N antibody positive rate was 48% , Both S and N antibodies positive were 21% , Both S and N antibodies negative were 22%. Co-detection S and N antibody was achieved 74% positive rate. Conclusion The methods we established here could be used for serological analysis of HCoV-HKU1. Either detection of HCoV-HKU1 S or N antibodies achieved good results. Higher positive detection rate of anti-S or anti-N antibody was found in the normal adults.