With the improvement of cataract operation, the cataract surgery has become increasingly perfect. The cataract patients show greater expectation for the result of cataract operation. As a result, refractive cataract surgery has become the main trend. Detailed investigations of corneal diseases, lens density, corneal topography, preferable intraocular lens ( IOL ) choice, and IOL power calculation can help us get a better knowledge of preoperative conditions on patients, which can be conducted with pentacam. So we can have a better forecast of post - operative outcome and improve the quality of vision for cataract patients after surgery.

To study the effect of a program of physical therapy,40 subjects with total hip replacement for femoral neck fracture were randomized into rehabilitation and control groups.The program included ROM exercises,low resistance exercises of hip abductors and ADL exercises.Muscle strength of hip abductors were measured by Kendall's Muscle Testing and Function,hip ROM,functional ability and pain were evaluated by Harris Hip score on the lst and 3rd month after surgery.Statistical analysis with t test was used for comparing Harris Hip scores.The result shows that muscle strength and hip function were improved significantly in rehabilitation group (p<0.05).That means the program is effective in long term after hip replacement.

Angiodysplasia ; Child, Preschool ; Humans ; Male ; Pulmonary Artery ; abnormalities ; Pulmonary Veins ; abnormalities

Objective: To observe the changes of plasma levels of omentin-1 and vaspin in patients with acute coronary syndrome (ACS). Methods: Our research included in 2 groups: ACS group, the patients with conifrmed diagnosis in our hospital from 2013-05 to 2013-09,n=100 including 52 male, 48 female and Control group, the patients with simultaneous coronary angiography excluded CAD,n=88. Plasma levels of omentin-1 and vaspin were examined by ELISA and the differences were compared between 2 groups. Results: Plasma levels of omentin-1 and vaspin in ACS group were lower than Control group (t=2.718,P<0.05) and (t=2.416,P<0.05). Correlation analysis presented that in ACS group, omentin-1 level was negatively related to total cholesterol (TC) (r=-0.702,P<0.05) and C-reactive protein (CRP) (r=-0.714,P<0.05); vaspin was negatively related to TC (r=-0.655, P<0.05) and CRP (r=-0.587,P<0.05); Omentin-1 was positively related to vaspin (r=0.643,P<0.05). Multiple linear stepwise regression analysis showed that plasma levels of omentin-1 and vaspin might be affected by CRP, TC and LDL-C. Conclusion: ACS patients had decreased plasma levels of omentin-1 and vaspin; in addition, omentin-1 and vaspin might be involved in lipid metabolism.

Objective To observe the expressions of stem cell factor(SCF) and macrophage colony-stimulating factor(M-CSF) in bronchoaveolar lavage fluid(BALF) of asthmatic mouse,and the effect of bacillus calmette-guerin(BCG) intervention on them.Methods Thirty Kunming rats were randomly divided into asthmatic group,BCG intervention group and control group,each group had 10 rats.Mice were sensitized and challenged with ovalbumin (OVA) to establish asthmatic model.Asthmatic mice were injected intradermally with BCG on 7,3,1 d before sensitization.After 24 h of last challenge,BALF were collected.The total cells and eosinophils(EOS) were counted in the BALF.The SCF and M-CSF levels of BALF were determined by enzyme-linked immunosorbent assay(ELISA).Results The number of total cells and EOS [(27.06?4.25)?107 L-1,(7.58?1.30)?107 L-1]in BALF in asthmatic mice were more than those in control group[(4.93?1.43)?107 L-1,0](Pa0.05).The M-CSF level also increased noticeably in asthmatic mice[(204.30?10.39) ng/L] compared with the control group[(181.33?8.63) ng/L](P

Objective To evaluate the efficacy of different processes ofBaijin Capsule to treatment of mice with chronic unpredictable stress-induced depression.Methods Chronic unpredictable stress was used to establish depression mice models. Experimental mice were divided into the following groups according to body mass: model group, positive medicine group, artware one (traditional process) high-dose and low-dose groups, and artware (refining process) two high-dose and low-dose groups. All medication groups were given relevant medicine for gavage. By detecting the two behavior indexes, immobility time of swimming and tail suspension of mice, the anti-depression effects of traditional process and refining process ofBaijin Capsule were investigated. By detecting autonomic activities and body mass, tested medicine was examined for function state of central excitation and changing animal body.Results The times for automatic activities ofBaijin Capsule in the model and artware (refining process) two high-dose and low-dose groups were (138.27±40.70)s, (100.01±34.75)s and (88.15±30.62)s, respectively, with statistical significance (P<0.01); the mice swimming immobility times in the model and artware (refining process) two low-dose groups were 668±19 and 705±24, respectively, with statistical significance (P<0.01); the mice tail suspension immobility times in model and artware (traditional process) one high-dose groups group were (28.14±1.25)g and (26.43±2.58)g, respectively, with statistical significance (P<0.05); the mice tail suspension immobility times in model and artware (refining process) two high-dose groups were (98.29±36.90)s and (87.54±30.05)s. Mice tail suspension immobility time in artware (refining process) two high-dose group decreased,without statistical significance.Conclusion Refining process ofBaijin Capsule can effectively be used to treat mice with chronic unpredictable stress-induced depression, and the efficacy is superior to the traditional process.

Adrenal Gland Diseases ; diagnosis ; pathology ; therapy ; Female ; Hemorrhage ; diagnosis ; pathology ; therapy ; Humans ; Infant, Newborn ; Magnetic Resonance Imaging ; Male ; Prognosis ; Tomography, X-Ray Computed

Protein-protein binding is the basis of virus and host cell interactions. With the application of technology of studying protein interactions, more knowledge of replication and pathogenesis of hepatitis C virus (HCV) was acquired. Non-structure protein 5B(NS5B) of HCV is a kind of viral protein, which plays an important role in replication of HCV. However, the effect of NS5B is not clear. To investigate the biological function of NS5B, we performed yeast two hybrid to look for proteins in hepatocytes interacting with NS5B. We constructed NS5B bait plasmid by cloning the gene of NS5B into pGBKT7, then transformed it into yeast AH109(a type). The transformed yeast was mated with yeast Y187(? type)containing liver cDNA library plasmid in 2?YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing x-?-gal for screening. Thirty-three colonies were selected and sequenced. Among them, two colonies were new genes with unknown function. The preliminary successful cloning of gene of protein interacting with NS5B paved the way for the study of the physiological function of NS5B and its associated protein.

OBJECTIVE:To investigate the dissolution of DEHP in PVC infusion set and the packaged infusion sets of the Paclitaxel injection so as to evaluate the safety of clinical infusion sets for paclitaxel.METHODS:The packaged infusion sets of Paclitaxel injection were collected from 9 Three-A grade hospitals in Chongqing.Paclitaxel injections were prepared as per actual concentration needed by the clinic and dripped through eight different infusion sets(in which 7 were packaged infusion sets) for 3 h.The peak area of the collected solution was measured and its content was determined by HPLC,and the effect of infusion time on the dissolution of DEHP was investigated as well.RESULTS:The dissolution of DEHP dripped through eight infusion sets were 1 408,9 393,6 576.5,2 412.6,8 194.4,0,8 477.2,and 8 037.4 ?g,respectively.The dissolution increased with the prolonging of infusion time.CONCLUSION:At present,the majority of packaged infusion sets of Paclitaxel injection were made form PVC materials.The safe medication of Paclitaxel can't be ensured for the dissolved DEHP can enter the blood directly,thus its hazardness should be given great attention.