1.Retroviral Expression System and Its Application in Efficient Foreign Gene Expression
Jian XU ; Shi-Chong LI ; Zhao-Lie CHEN ;
China Biotechnology 2006;0(05):-
Retroviral expression system which consists of retroviral vector,envelop protein vector and packaging cell line is an efficient expression system for recombinant protein.It has great potential in gene therapy and biopharmacy.Transcriptional active genome regions are the preferred targets for retrovirus integration.Furthermore,VSV-G protein enables this system a broader host range and makes virus integration more efficient.After infection of high-titer virus,high production clones can be selected through simple screening.So far,the research on retrovirus expression system has developed into application in bio-pharmacy industry.Here the composition of this system and the mechanism of virus transduction and summarize the application and prospect of retroviral expression system are introduced.
3.Study on inhibitory effect of calycosin on hepatic stellate cell activation in rats by up-regulating peroxisome proliferator-activated receptor γ.
Jian PING ; Hong-yun CHEN ; Yang ZHOU ; Gao-feng CHEN ; Lie-ming XU ; Yang CHENG
China Journal of Chinese Materia Medica 2015;40(12):2383-2388
To observe the effect of calycosin on the proliferation and activation of primary hepatic stellate cells (HSCs) in rats, and prove calycosin shows the effects through peroxisome proliferator-activated receptor γ(PPARγ) and farnesoid X receptor (FXR). The results indicated that calycosin could inhibit HSC proliferation and expressions of activation marker smooth muscle actin-α and type I collagen. With the increase in HSC activation time, FXR expression reduced, but with no notable impact from calycosin. Calycosin could up-regulate PPARγ expression and its nuclear transition in a concentration-dependent manner. Its prohibitory effect on HSC activation could be blocked by PPARγ antagonist. In conclusion, calycosin could inhibit HSC activation and proliferation, which may be related with the up-regulation of PPARγ signal pathway.
Animals
;
Cell Proliferation
;
drug effects
;
Cells, Cultured
;
Drugs, Chinese Herbal
;
pharmacology
;
Hepatic Stellate Cells
;
cytology
;
drug effects
;
metabolism
;
Isoflavones
;
pharmacology
;
Male
;
PPAR gamma
;
genetics
;
metabolism
;
Rats
;
Rats, Sprague-Dawley
;
Receptors, Cytoplasmic and Nuclear
;
genetics
;
metabolism
;
Up-Regulation
;
drug effects
4.Effect of Yiguan Decoction on differentiation of bone marrow mesenchymal stem cells into hepatocyte-like cells: an experimental research.
Jian PING ; Hong-Yun CHEN ; Zhou YANG ; Cheng YANG ; Lie-Ming XU
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(3):348-354
OBJECTIVETo observe the effect of Yiguan Decoction (YGD) on differentiation of bone marrow mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro.
METHODSRat BMSCs were isolated using whole bone marrow adherent method. The properties of BMSCs were identified by analyzing the expression of surface cytokines by flow cytometry. The third passage cells were differentiated into fat cells to identify their features. BMSCs were incubated with hepatocyte growth factor (HGF) plus fibroblast growth factor 4 (FGF4) or YGD containing serum YGD for 21 days. The mRNA expression of alpha-fetoprotein (alphaAFP), albumin (Alb), and hepatocyte nuclear factor 4alpha (HNF4alpha) were detected by real time PCR. Expression of AFP and cytokeratin 18 (CK18) protein was detected by cell immunofluorescence. Glycogen synthesis was observed using periodic acid-Schiff stain (PAS). CK18, Wnt 3alpha, and alphacatenin protein expressions were detected by Western blot.
RESULTSHigh expression of CD90, CD29, and CD44, and low expression of CD34 and CD11b were observed in BMSCs isolated by whole bone mar- row adherent method, and numerous lipid droplets were observed in BMSCs using oil red O staining. Both YGD containing serum and growth factor stimulated the expression levels of Alb, AFP, HNF4alpha mRNA and CK18 protein. The down-regulated expression of Wnt 3alpha and beta-catenin could be detected at 21 days after induction. The synthesized glycogen granule could be seen. Down-regulated Wnt 3alpha and beta-catenin expression could also be observed.
CONCLUSIONYGD could induce the differentiation of rat BMSCs into hepatocyte-like cells, which was related to down-regulating Wnt/beta-catenin signal pathway.
Animals ; Bone Marrow Cells ; cytology ; drug effects ; Cell Differentiation ; drug effects ; Cells, Cultured ; Drugs, Chinese Herbal ; pharmacology ; Hepatocytes ; cytology ; Male ; Mesenchymal Stromal Cells ; cytology ; drug effects ; Rats ; Rats, Sprague-Dawley ; Wnt Signaling Pathway
5.Immune Responses of Th1/Th2 in Children with Mycoplasma Pneumoniae Pneumonia
jian-min, CAI ; zhen-hai, WANG ; hong-lie, ZHOU ; tang-xin, CHEN
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To study the immune responses of Th1 and Th2 subsets of T cells in 26 children with mycoplasma pneumo-niae pneumonia(MPP).Methods ELISA was used to detect the levels of interferon- ?( IFN- ?) and interleukin-4(IL-4) in the serum in 12 healthy children as normal controls and 26 patients of acute stage as acute stage MPP group, 9 of whom in recovery stage were as recovery stage MPP group. Results IFN - ? level in acute stage MPP group was significant higher than that in normal controls (P 0.05); and IFN - 7/IL - 4 ratio was significant higher than that in normal controls( P0.05).Conclusion Thl responses increase and Th2 responses decrease in children with MPP,and this kind of response persists during the recovery stage.
6.Significance of Interleukin-10 and Transforming Growth Factor-beta 1 Detection in Children with Mycoplasma Pneumoniae Pneumonia
jian-min, CAI ; tong-xin, CHEN ; zhen-hai, WANG ; hong-lie, ZHOU
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To explore the changes of interleukin-10(IL-10) and transforming growth factor-beta 1(TGF-?1)in 26 children with mycoplasma pneumoniae pneumonia(MPP).Methods Enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of IL-10 and TGF-?1 in the serum of 12 healthy children as normal controls and 26 patients of acute stage as acute stage MPP group,9 of acute stage MPP group in recovery stage as recovery stage MPP group.The levels of IL-10 and TGF-?1 were compared between the three groups.Results IL-10 level in acute stage MPP group was significantly lower than that in normal controls(P
7.Evaluation and clinical significance of HBV large protein (LHBs) in diagnosis of hepatitis B.
Yong-Jian CHEN ; Yong-Lie ZHOU ; Jun XIA ; Wei-Wei XIA
Chinese Journal of Experimental and Clinical Virology 2007;21(3):241-243
OBJECTIVETo explore the significance of HBV large protein (LHBs) in diagnosis of hepatitis B, we detected the LHBs, HBV DNA, PreS1 and other hepatitis B viral markers (HBV M) in the serum of patients infected with HBV.
METHODSHBV DNA was quantitatively detected using RT-PCR, LHBs, PreS1 and HBV M were analyzed by ELISA in totally 385 serum samples.
RESULTSThere was a significant difference between the positive rate of LHBs (86.97%) and PreS1 (49.5%) in the 307 serum positive for HBV DNA (P less than 0.05). There was a correlation between the levels of LHBs and the logarithm of HBV DNA (r=0.935). In the serum specimens of patients negative for HBeAg, there was no significant difference between the positive rate of LHBs (76.92%) and the HBV DNA (67.95%), but the positive rate of PreS1 (45.73%) was lower than that of LHBs or HBV DNA.
CONCLUSIONThere was a close correlation between the copies of HBV DNA and the levels of LHBs, both the positive rate and the coincidence rate of LBHs and HBV DNA were higher than those of PreS1. LHBs can reflect the replication status of HBV.
Adolescent ; Adult ; Aged ; DNA, Viral ; blood ; genetics ; Female ; Hepatitis B ; blood ; diagnosis ; Hepatitis B Surface Antigens ; blood ; Hepatitis B virus ; genetics ; immunology ; Humans ; Male ; Middle Aged ; Protein Precursors ; blood ; Reverse Transcriptase Polymerase Chain Reaction ; Sensitivity and Specificity ; Viral Matrix Proteins ; blood ; Young Adult
8.Immunophenotype of solid pseudopapillary tumor of pancreas and its pathological indication.
Ying CHEN ; Guan-zhen YU ; Da-lie MA ; Can-rong NI ; Jian-ming ZHENG ; Ming-hua ZHU
Chinese Journal of Pathology 2006;35(8):488-489
Actins
;
analysis
;
Antigens, CD34
;
analysis
;
Carcinoma, Papillary
;
classification
;
metabolism
;
pathology
;
Female
;
Humans
;
Immunohistochemistry
;
Keratin-19
;
analysis
;
Keratin-20
;
analysis
;
Muscle, Smooth
;
chemistry
;
Pancreatic Neoplasms
;
classification
;
metabolism
;
pathology
;
Proto-Oncogene Proteins c-kit
;
analysis
;
Receptors, Estrogen
;
analysis
;
Receptors, Progesterone
;
analysis
9.Histiocytic sarcoma of stomach: report of a case.
Ting FENG ; Miao-xia HE ; Wei-yong GU ; Chen-guang BAI ; Da-lie MA ; Jian-ming ZHENG ; Ming-hua ZHU
Chinese Journal of Pathology 2012;41(2):130-131
Aged
;
Antigens, CD
;
metabolism
;
Antigens, Differentiation, Myelomonocytic
;
metabolism
;
Carcinoma, Large Cell
;
metabolism
;
pathology
;
Diagnosis, Differential
;
Histiocytic Sarcoma
;
metabolism
;
pathology
;
surgery
;
Hodgkin Disease
;
metabolism
;
pathology
;
Humans
;
Lymphoma, Large B-Cell, Diffuse
;
metabolism
;
pathology
;
Lymphoma, Large-Cell, Anaplastic
;
metabolism
;
pathology
;
Male
;
Melanoma
;
metabolism
;
pathology
;
Receptors, Cell Surface
;
metabolism
;
Stomach Neoplasms
;
metabolism
;
pathology
;
surgery
10.Study on the mechanism of the interaction between montmorillonite and bacterium.
Xiu-rong HU ; Guang-lie LU ; Lin-shen CHEN ; Jian-ming GU ; Yue ZHANG
Acta Pharmaceutica Sinica 2002;37(9):718-720
AIMTo investigate the mechanism of the interaction between montmorillonite and bacteria by studying the reactions of different charges of montmorillonites with bacteria.
METHODSBacteriostatic test: one loop of E. coli and Staphylococcus aureus at the concentration of 1 x 10(6).mL-1 was incubated to the plate culture medium containing different concentrations of montmorillonite, and incubated 24 h to observe the growth of bacteria. Bacterial adsorptive test: different amounts of montmorillonite were added into the artificially simulated intestinal solution (containing bacteria 1 x 10(7).mL-1). After the culture, the bacterial colonies were counted.
RESULTSThe results showed that montmorillonite per se showed no bacteriostatic or bactericidal effect, but after exchange with metal ion and functional groups which inhibits bacteria, then it showed these activities. Adsorption was the main way between montmorillonite and bacteria. The special way of fixing bacteria into the "carriage" of montmorillonite gel which carry this structure was its pharmacological basis of curing diarrhea. The adsorption effect was related to layer charge density of the montmorillonites.
CONCLUSIONMontmorillonite showed adsorption ability of bacteria with minus related to its layer charge, but has no bacteriostatic and bactericidal effect.
Adsorption ; Anti-Ulcer Agents ; pharmacology ; Bentonite ; pharmacology ; Escherichia coli ; drug effects ; Staphylococcus aureus ; drug effects