1.Preparation and anti-cancer activity in vitro of curcumin loaded mesoporous silica nanoparticle.
China Journal of Chinese Materia Medica 2015;40(21):4189-4193
This paper is to prepare curcumin (Cur) loaded mesoporous silica nanoparticle (Cur-MSN), evaluate its release behavior and anti-cancer activity in vitro. Mesoporous silica nanoparticle (MSN) was prepared by polymerization method and Cur-MSN was obtained using solvent evaporation method and impregnation centrifugation method. The preparation method was optimized using entrapment efficiency (EE) and loading efficiency (LE) as indexes. Cur-MSN was characterized with scanning electron microscope and its particle size and zeta potential were determined. Finally, in vitro release behavior in 0.2% SDS solution and its cell-killing effect on HeLa cells were also evaluated. The Cur-MSN prepared with process optimization method was round and uniform and exhibited typical mesoporous characterization. The mean particle size and Zeta potential of Cur-MSN were 75.8 nm and -30.1 mV, respectively. EE and LE of three batches of Cur-MSN were (72.55 ± 2.01)% and (16.21 ± 1.12)%, respectively. In vitro release behavior of Cur-MSN showed a sustained release profile with 83.5% cumulative release within 96 h. The killing effect of Cur-MSN on HeLa cells was dose-dependent with IC50 of 19.40 mg x L(-1), which was similar to that of Cur.
Antineoplastic Agents, Phytogenic
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chemistry
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pharmacology
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Curcumin
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chemistry
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pharmacology
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Drug Carriers
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Nanoparticles
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chemistry
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Neoplasms
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drug therapy
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Particle Size
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Porosity
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Silicon Dioxide
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chemistry
3.Langerhans' cell histiocytosis.
Chinese Journal of Pathology 2005;34(11):752-753
Antigens, CD
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metabolism
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Antigens, CD1
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metabolism
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Antigens, Differentiation, Myelomonocytic
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metabolism
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Diagnosis, Differential
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Histiocytosis, Langerhans-Cell
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metabolism
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pathology
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Histiocytosis, Sinus
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pathology
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Humans
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Infant
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Langerhans Cells
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pathology
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Lymph Nodes
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pathology
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Lymphohistiocytosis, Hemophagocytic
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pathology
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Male
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S100 Proteins
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metabolism
4.Inducible Nitric Oxide Synthase and p53 Expression in Hepatocellular Carcinoma and Their Relationship with Angiogenesis
Jian ZHANG ; Sheng HE ; Maode LI ;
Chinese Journal of Bases and Clinics in General Surgery 2003;0(05):-
Objective To investigate the expression of inducible nitric oxide synthase (iNOS) and p53 protein in hepatocellular carcinoma (HCC) and their relationship with angiogenesis. Methods Immunohistochemical method and image analysis technique were used to detect the expression of iNOS and p53 protein in tumor tissue sections of 59 HCC patients. Microvessel density (MVD) was counted by immunohistochemical staining with anti CD34 antibody.Results ①The expression rates of iNOS and p53 were 81.4%(48/59), 64.4%(38/59) in HCC patients, respectively. The expression intensities of iNOS and p53 were 5 635?1 287, 3 352?873 in HCC patients, respectively. ②MVD was 32.5?2.73 in the tumor tissue of HCC patients. ③The expression of iNOS was correlated with the expression of p53 and MVD in HCC patients ( P
5.Determination of Kaempferol-7-O-?-D-glucopyranoside in Hosta ventricosa Stearn by HPLC
Jian HE ; Ying GAO ; Weimin LI
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To establish a HPLC method for the determination of kaempferol-7-O-?-D-glucopyranoside in Hosta ventricosa stearn.Methods HPLC ana1ysis was carried out on a Phenomenex Luna C18(250 mm?4.6 mm,5 ?m) column,gradient elution with the mobile phase of 0.5 % phosphate acid in water-methanol.The detective wavelength was set at 364 nm.Results calibration curve was linear within the range of 0.113 ~ 2.260 ?g for kaempferol-7-O-?-D-glucopyranoside,coefficient correlation being 1.The average recovery was 101.1 %(RSD= 2.58 %,n=6).Conclusion The content of kaempferol-7-O-?-D-glucopyranoside in Hosta ventricosa stearn is 0.2 mg/g(0.02 %) on average.This method is simple,accurate and practical for the determination of kaempferol-7O-?-D-glucopyranoside.
6.The comparison of the diagnostic values of 18F-FDG-SPECT and helical CT in the detection of the metastasis of postoperative breast cancer
Jian HE ; Suping LI ; Gongshun TANG
Journal of Chinese Physician 2015;17(8):1164-1166,1169
Objective To compare the diagnostic values of 18F-fluorodeoxyglucose-single photon emission computed tomography (18F-FDG-SPECT) and helical CT in the detection of the metastasis of postoperative breast cancer.Methods A total of 94 patients with postoperative breast cancer were chosen as research objects.The follow-up duration of post-operation was 2 years.All patients were received by 18F-FDG-SPECT and helical CT during follow-up.Lymph nodes that were suspected to the postoperative metastasis were taken for histological procedure.The diagnostic values of 18F-FDG-SPECT and helical CT to the metastasis of postoperative breast cancer were compared.Results Compared to helical CT,the sensitivity,specificity,positive predictive value,negative predictive value,and positive likelihood ratio of 18F-FDG-SPECT were higher (P < 0.05);however,the negative likelihood ratio of 1s F-FDG-SPECT were lower (P < 0.05).Conclusions 18F-FDG-SPECT has more important clinical value in the detection of metastasis of postoperative breast cancer relative to helical CT.
7.Analysis of the cause of hemorrhage after MPCNL and its interventional treatment
Yongzhong HE ; Jian-He LIU ; Guo-Hua ZENG ; Jian YUAN ; Xun LI ; Zhao-Hui HE
Chinese Journal of Urology 2001;0(06):-
Objective To analyze the cause of delayed hemorrhage after minimally invasive percu- taneous nephrolithotomy(MPCNL),and to summarize the experience in the interventional treatment of severe bleeding after MPCNL by superselective arteriolar embolization.Methods The clinical data of 3812 cases of MPCNL from June 1998 to July 2004 were reviewed.Of them,12 patients(11 men and 1 woman;mean age,45 years)who developed severe hemorrhage after MPCNL were identified.The cause of hemorrhage and the treatment results were analyzed.Results The rate of delayed hemorrhage after MPCNL was 0.31% (12/3812).The mean time to onset of severe bleeding was 10 d after MPCNL.Renal arteriography was per- formed in all 12 patients,showing 5 arteriovenous fistulas and 7 false aneurysms.Superselective arteriolar em- bolization for hemostasis was performed in all 12 cases.All these vascular abnormalities were successfully treated by superselective embolization.Follow-up showed that the hematuria disappeared and renal function recovered well.Conclusions Severe hemorrhage following MPCNL is a rare complication,the incidence of which is significantly lower than that of conventional PCNL.The cause is mainly the arteriolar injury of re- nal puncture passage.Superselective embolization provides effective control of bleeding and currently consti- tutes the treatment of choice based on our experience.
8.Study on the relation of plasma D-dimer levels of fracture patients and their situation
Li YAN ; Zhiping LI ; He LEI ; Jian YUE ; Miaomiao WANG
Chinese Journal of Primary Medicine and Pharmacy 2013;20(z1):11-12
Objective Through the two D-dimer content detection of the fracture patients and healthy con-trols,and analyze the difference between them ,to explore the correlation between fractures were detected two D-dimer and the importance and the patient′s condition.Methods 306 of fracture patients were collected for determination of D-dimer content were detected at the same time ,100 healthy people as control two D-dimer content;analysis of the correlation between the fracture in 306 cases patients with D-dimer content .Results The plasma D-dimer level of pa-tients with two fracture(10.7 ±2.6)mg/L,which was significantly higher than that of healthy subjects (0.3 ±0.1) mg/L(t=11.07,P<0.01);The fracture number,fracture patients plasma D-dimer detection results content is high-er,the more serious condition .Conclusion The plasma D-dimer level was significantly higher in patients with two fractures,coagulation Stata ,fibrinolytic enhancement ,easily lead to venous thrombosis and disseminated intravascular coagulation in patients with more complications such as fracture;fracture,the higher the content of plasma D-dimer, patients with more serious ,there is correlation .
9.SLOT Scan imaging in teenagers with scoliosis
Weijun SITU ; Yajun LI ; Zuohua LI ; Zhongjun HU ; Jian HE
Journal of Central South University(Medical Sciences) 2009;34(7):675-679
Objective To explore the improvement effect of SLOT Scan technology (narrow seam exposure capture technology) and the radiography techniques on the quality of the scoliosis X-ray films in teenagers. Methods The Sonialvision Satire Ⅱ equipment of Shimadzu corporation and SLOT Scan were applied to take the radiography for 60 patients taller than 1.50 metres. All the data were collected through a continual exposure, and the images were sewn up through a seamless connec-tion software. Results Cervical, thoracic, and lumbar and sacral segments could clearly present at the same time on one X-ray film by seamless splicing, and the quality of one time radiograph was as good as one film. Conclusion As a new radiology technique, SLOT Scan can wipe off splitting arti-fact effectively and make the whole spine seamless present on one X-ray film. It helps the spinal sur-geons to observe, calculate and measure accurately. It is useful to choose the operation mode and judge the curative effect.
10.Effect of Cilostazol on the Ion Channel of Right Ventricular Cells in Experimental Rats
Rong HE ; Guangping LI ; Jian LI ; Lijun CHENG
Chinese Circulation Journal 2015;(1):54-58
Objective: To observe the effect of cilostazol on the ion channel of right ventricular cells in experimental rats, and to explore the ion channel mechanism of ciolstazol for preventing the ventricular arrhythmia in Brugada syndrome.
Methods: Our research was composed of 2 groups: ①Perfusion group, the cells were treated in 4 sub-groups by cilostazole at 1, 2, 5, 50μmol/L respectively, and there were 9, 5, 3, 7 cells were recorded at each sub-group to observe the differences of current density Ito at before and after treatment. ②Oral group, which included 4 sub-groups:Control 1 with 7 rats, Experiment 1 with 5 rats, and Control 2 with 8 rats, Experiment 2 with 6 rats respectively. The differences of current density Ito and ICa,L were studied between each Control and Experiment sub-groups.
Results: In Perfusion group,①with cilostazole 1, 2, 5, 50μmol/L treatment, the current density Ito decreased in all sub-groups, when the self-command voltage at+60mV, the Ito was signiifcantly different in each sub-group at before and after treatment, all P<0.05.②When each command voltage decreasing, the reduction rates of Ito were similar among 4 sub-groups, all P>0.05. In Oral group,①When the self-command voltage from-50mV reached the maximum of+60mV, the Ito was similar between Control 1 and Experiment 1 sub-groups, P>0.05.②When the self-command voltage at+10mV, the current density of ICa,L was slightly higher in Control 2 sub-group than that in Experiment 2 sub-group, P>0.05.
Conclusion: Direct perfusion of cilostazole in right ventricular cells may inhibit Ito in experimental rats, such effect was similar with cilostazole treatment at (1-50)μmol/L. Cilostazole might prevent the ventricular arrhythmia in Brugada syndrome in experimental rats.