2.Clinical research on second electric transurethral resection for noninvasive bladder cancer
Ying LIU ; Jian JIN ; Xishuang SONG ; Qizhong FU ; Hui CHI
Chinese Journal of General Practitioners 2009;8(8):541-543
iple occurrence of the tumor, which can decline its recurrence and postpone its progression.
3.STUDY ON THE ANTIFUNGAL ACTIVITIES OF ENDOPHYTIC FUNGI ISOLATED FROM SEVERAL PHARMACEUTICAL PLANTS
Gui-Ling LI ; Jian-Feng WANG ; Yao-Jian HUANG ; Zhong-Hui ZHENG ; Wen-Jin SU ;
Microbiology 1992;0(06):-
One hundred and seventy-two strains of endophytic fungi were isolated from Taxus mairei,Cephalotaxus fortunei and Torreya grandis cv.merrillia.The result of the antifungal assay shows that ninety strains of the fungi have antagonism against one or more botanical pathogenic fungi,such as Neurospora sp.,Trichoderma sp.,Fusarium sp.etc.The percentage of antifungal strains to tested strains are as follows:40% Cephalotaxus fortunei,54.2% Taxus mairei,57.1% Torreya grandis cv.merrillia.Thirty-five strains have high antifungal activities,and their inhibition zone diameter is at least 15mm.The active endophytic fungi were identified as 18 genera,most of which belong to Paecilomyces and Fusarium etc.
4.Comparison between hemoperfusion and hemodialysis-hemoperfusion-line for treatment of severe organophosphorous pesticide poisoning.
Jin-zhong FENG ; Jian ZHANG ; Zhi-jin YU ; Wei-ping ZHENG ; Jing-hui ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(6):377-378
Female
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Hemoperfusion
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Humans
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Male
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Organophosphate Poisoning
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Pesticides
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poisoning
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Poisoning
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therapy
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Renal Dialysis
5.Regulatory effect of Flos Abelmoschus manihot in mice with inflammatory bowel disease based on gut microbiota sequencing and untargeted lipidomics
Shu-hui YANG ; Cheng-xi LI ; Jian-ping LI ; Yu-meng WANG ; Yun LIU ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2022;57(12):3546-3556
In this study, the ameliorative effects of
6.1H-NMR based metabonomic approach to evaluate detoxification effect of vinegar-processed Euphorbia kansui.
Yu-Mei LIU ; Rong-Rong HUI ; Cui-Cui HE ; Jin-Ao DUAN ; Jian-Xin LI
China Journal of Chinese Materia Medica 2014;39(2):322-326
Euphorbia kansui (EK) is a toxic herbal drug, and often used after vinegar-processing to reduce its toxicity. In present study, a 1H-NMR based metabonomic approach was used to evaluate the detoxification effect of vinegar-processed EK. The water extracts of EK and VEK were administered orally to male SD rats at doses of 9 g x kg(-1) x d(-1) for 1 week, respectively, and one more week observation was further conducted. The control group was orally given with saline. Histopathological studies of liver samples on the 8th and 15th day were conducted, and the metabolites of rat urine and liver were analysed by 1H-NMR. Histopathological studies of liver samples from EK and VEK treated rats showed no negative impacts. In metabonomic analyses of urines, changes of metabolites indicated liver damages, kidney lesions and imbalance of gut microbes in the second week. VEK-treated rats showed a quite lower toxicity compared with EK-treated ones. The present study revealed that the metabonomic approach might be helpful for the evaluation of toxicity of EK and detoxic effect of VEK.
Acetic Acid
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chemistry
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Animals
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Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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pharmacokinetics
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toxicity
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Euphorbia
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chemistry
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Inactivation, Metabolic
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Liver
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drug effects
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metabolism
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Magnetic Resonance Spectroscopy
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Male
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Metabolomics
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methods
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Rats
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Rats, Sprague-Dawley
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Urinalysis
7.Optimization of Extraction Process for Albizia julibrissin in Baijin Capsule by Orthogonal Test
Hui ZHANG ; Jin ZHANG ; Juanjuan QI ; Xiaofang DANG ; Sali CAO ; Jian NI
Chinese Journal of Information on Traditional Chinese Medicine 2014;(3):78-80
Objective To optimize the extraction process of Albizia julibrissin in Baijin Capsule. Methods Using the extraction rate of quercitrin and total flavonoids as indexes, the orthogonal design was used to investigate effects of solvent volume, extraction time and extraction frequency on extraction results. Results The optimal extracting condition was as follows:extracted 2 times with 12 fold 70%alcohol, 2 h for each time. Conclusion The optimized process condition was simple, stable and feasible. It provides the basis for the production of Baijin Capsule.
8.Effects of glycyrrihizic acid and prednisone on pathological and ultrastructural changes of kidney in rats with chronic aristolochic acid nephropathy.
Hui-ling WANG ; Jin-yuan ZHANG ; Jian HUANG
Chinese Journal of Integrated Traditional and Western Medicine 2007;27(1):45-49
OBJECTIVETo investigate the effects of glycyrrihizic acid (GA) and prednisone on renal injury in chronic aristolochic acid nephropathy (AAN) rat model.
METHODSNinety-eight male Wistar rats of SPF grade were randomly divided into 4 groups, the normal control group (n = 20), the GA group, the model group, and the prednisone group, 26 rats in each group. Rats in the latter 3 groups were made into AAN model by administration of aristolochic acid (AA, contained in extract of Caulis Aristolochiae Manshuriensis) 20 mg/(kg x d) by gastric gavage, and equal volume of drinking water was given to rats in the control group. Medication was started 2 h later, the GA group was treated with GA 25 mg/(kg x d), the prednisone group with prednisone 3.15 mg/(kg x d), and to the other two groups equal volume of drinking water was given. Body weight was measured weekly, renal function related indices and morphology of the renal tissue were examined at the 4th, 8th and 12th weekend.
RESULTSAlong with the feeding time, body weight in the control group increased steadily, while that in the treated groups increased slowly. The ratio of serum creatinine/body weight increased markedly in the model group, while it significantly lowered in the treated groups. Morphological examination showed that the structural injury in the treated groups was milder than that in the model group, and its degree of fibrosis was milder also (15% - 20% vs 30%). Electronmicroscopy showed that AA induced, injury degeneration and necrosis of renal tubular epithelial cells, and markedly injured the cell organs, such as mitochondria, and induced nuclear variation, while in the treated groups, it was mainly limited in renal tubule, with normal cell organs, few nucleolus variation and less interstitial collagen fibers.
CONCLUSIONGA and prednisone could reduce the serum creatinine level, improve renal function, relieve the renal morphological changes, and decrease the inter stitial fibrosis, showing a definite preventing effect on chronic AAN in rats.
Animals ; Anti-Inflammatory Agents ; therapeutic use ; Aristolochic Acids ; Chronic Disease ; Drug Therapy, Combination ; Glycyrrhetinic Acid ; therapeutic use ; Kidney ; drug effects ; pathology ; ultrastructure ; Kidney Diseases ; chemically induced ; drug therapy ; Male ; Microscopy, Electron ; Prednisone ; therapeutic use ; Random Allocation ; Rats ; Rats, Wistar ; Treatment Outcome
9.Combined surgery of open and ultrasound-guided Mammotome in the treatment of multiple breast lumps
Hongliang CHEN ; Ang DING ; Jian SUN ; Yuchun JIN ; Taiming SUN ; Maoli WANG ; Hui SONG
Fudan University Journal of Medical Sciences 2009;36(4):417-421
Objective To evaluate the effect and clinical value of open surgery combined ultrasound-guided Mammotome in the treatment of multiple breast lumps. Methods Four hundred and forty-four patients in our hospital from Jan. 2006 to Jun. 2008 were divided into 3 groups, who underwent classical open surgery, ultrasound-guided Mammotome operation, or combined therapy respectively and followed by post-operation visits reguarly. The operation effects were compared between the 3 groups. Results Compared with the classical open surgery, combined therapy had no difference in time of procedure and procedural bleeding, but had lower incidence of local skin, better incision condition and higher satisfaction of patients. Compared with ultrasound-guided Mammotome operation, combined therapy took less time in procedure, and in the same time had less procedural bleeding, lower post-operation complication and higher patients satisfaction. Conclusions Combined therapy has high complete removal rate, low post-operation complication as well as cosmetic effect. It has special advantages over the other two kinds of surgery, so it has wide clinical application.
10.Association of genetic polymorphisms in the FGB promoter region with idiopathic deep venous thrombosis
Shengbin HAN ; Jian DONG ; Hui JIN ; Bin YANG ; Fang YIN ; Youli WANG
Chinese Journal of General Surgery 2015;30(4):272-275
Objective To probe the association between possible single nucleotide polymorphism (SNP) in the FGB promoter region and idiopathic deep venous thrombosis.Methods A prospective analysis was performed in both IDVT group and control group (120 cases each) followed by a duplex examination using gene sequencing technique and restriction fragment length polymorphism (RFLP) in the promoter region of fibrinogen gene β.Possible SNPs in this region were detected arranged before HardyWeinberg equilibrium test and Linkage disequilibrium (LD) analyses.Ultimately,we compared the genotype frequencies between the two groups and undertook a multiple Logistic regression.Results Six kinds of SNPs were determined in the promoter region of β-fibrinogen gene:-148C/T,-249C/T,-455G/A,-854G/A,-993C/T and-1420G/A.A stronger linkage disequilibrium was confirmed between-993C/T and -455G/A (r2 =0.699) ;-993C/T and-148C/T (r2 =0.509) ;-455G/A and-148C/T (r2 =0.556).Statistical differences of genotype frequencies between two groups were observed in-148C/T,-249C/T,-455G/A and-1420G/A polymorphisms (all P < 0.05).Conclusions The risk of IDVT was 4.579 times higher with every 1 g/L increase of fibrinogen concentration.Allele-148T,-455G and-1420A are IDVT risk factors.-993C/T may indirectly affect IDVT through linkage disequilibrium with-455G/A and-148C/T.