2.Clinical research on second electric transurethral resection for noninvasive bladder cancer
Ying LIU ; Jian JIN ; Xishuang SONG ; Qizhong FU ; Hui CHI
Chinese Journal of General Practitioners 2009;8(8):541-543
iple occurrence of the tumor, which can decline its recurrence and postpone its progression.
3.STUDY ON THE ANTIFUNGAL ACTIVITIES OF ENDOPHYTIC FUNGI ISOLATED FROM SEVERAL PHARMACEUTICAL PLANTS
Gui-Ling LI ; Jian-Feng WANG ; Yao-Jian HUANG ; Zhong-Hui ZHENG ; Wen-Jin SU ;
Microbiology 1992;0(06):-
One hundred and seventy-two strains of endophytic fungi were isolated from Taxus mairei,Cephalotaxus fortunei and Torreya grandis cv.merrillia.The result of the antifungal assay shows that ninety strains of the fungi have antagonism against one or more botanical pathogenic fungi,such as Neurospora sp.,Trichoderma sp.,Fusarium sp.etc.The percentage of antifungal strains to tested strains are as follows:40% Cephalotaxus fortunei,54.2% Taxus mairei,57.1% Torreya grandis cv.merrillia.Thirty-five strains have high antifungal activities,and their inhibition zone diameter is at least 15mm.The active endophytic fungi were identified as 18 genera,most of which belong to Paecilomyces and Fusarium etc.
4.Comparison between hemoperfusion and hemodialysis-hemoperfusion-line for treatment of severe organophosphorous pesticide poisoning.
Jin-zhong FENG ; Jian ZHANG ; Zhi-jin YU ; Wei-ping ZHENG ; Jing-hui ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(6):377-378
Female
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Hemoperfusion
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Humans
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Male
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Organophosphate Poisoning
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Pesticides
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poisoning
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Poisoning
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therapy
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Renal Dialysis
5.Regulatory effect of Flos Abelmoschus manihot in mice with inflammatory bowel disease based on gut microbiota sequencing and untargeted lipidomics
Shu-hui YANG ; Cheng-xi LI ; Jian-ping LI ; Yu-meng WANG ; Yun LIU ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2022;57(12):3546-3556
In this study, the ameliorative effects of
6.Effects of Neuromuscular Electrical Stimulation Combined with Strength Training on Motor Function in Children with Spastic Cerebral Palsy
hui-ci, LIANG ; kai-shou, XU ; lu, HE ; jin-ling, LI ; jian-ning, MAI
Journal of Applied Clinical Pediatrics 2004;0(12):-
0.05).Compared with the CSS,GMFM and WV before treatment,there were statistically difference after 6 and 12 weeks treatment in two groups(Pa
7.Clinical study on colonic transmission time and the effect of sini powder on it in functional constipation patients.
Zhao-hui JIN ; Jian-hua DUAN ; Hong-chuan ZHAO
Chinese Journal of Integrated Traditional and Western Medicine 2006;26(10):896-898
OBJECTIVETo investigate the characteristic of colonic transmission in functional constipation (FC) and the effect of traditional Chinese medicine (TCM) Sini Powder (SP) on it.
METHODSThe colonic transmission time (CTT) of 36 patients with FC (the FC group) and 22 healthy subjects (control group) was measured through colonic transmission test, and CTT of entire colon and that of various subsections was calculated with Hinton method and Arhan method respectively. After then, the FC group was treated with SP for 7 days, and CTT was detected again after treatment.
RESULTSBefore treatment, body mass index (BMI) was higher, CTT of entire colon, left half colonic section, and sigmoid-rectal section were longer in the FC group than those in the control group (P < 0.05), no statistical difference in CTT of right half colon was found between the two groups (P > 0.05). After FC patients being treated with SP, their CTT of whole colon, left half colonic section and sigmoid-rectal section were significantly shortened (P < 0.05).
CONCLUSIONFC patients were characterized by increased BMI and CTT prolonged and unevenly distributed in subsections, especially in the left half colon, sigmoid and rectum; SP could shorten the CTT in FC patients.
Adult ; Body Mass Index ; Colon ; drug effects ; physiopathology ; Colon, Sigmoid ; drug effects ; physiopathology ; Constipation ; drug therapy ; physiopathology ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Gastrointestinal Transit ; drug effects ; Humans ; Male ; Middle Aged ; Phytotherapy ; Time Factors ; Treatment Outcome
8.Experimental study on formation of neutrophil extracellular traps in human peripheral blood induced by paraquat in vitro
Wei JIN ; Jian LU ; Hui XIE ; Yiqun JIANG ; Xiaoxiao MENG ; Yong ZHU ; Ruilan WANG
Chinese Critical Care Medicine 2017;29(3):216-220
Objective To explore whether paraquat (PQ) can induce the formation of neutrophil extracellular traps (NETs) in human peripheral blood.Methods Neutrophils were isolated from healthy human peripheral blood,and the cells were identified by hematoxylin-eosin (HE) strain.The cells were treated with different concentrations of PQ [0 (as control),200,400,600,800,1 000 and 1 200 μmol/L],and the cell viability was measured by cell proliferation and CCK-8 cytotoxicity detection kit,and the median lethal concentration of PQ was selected.The cells were treated with the median lethal concentration of PQ (PQ poisoning group),and the untreated cells were served as the control.Immunofluorescence staining was adopted to evaluate NETs formation.PicoGreen dye was used to determine the quantitative content of circulating free DNA.Western Blot was used to determine the expressions of citrullinated histone 3(H3Cit) and myeloperoxidase (MPO) in the supernatant.Results The purity of neutrophils was about 95% by HE staining.The cells were treated with different concentrations of PQ,and the result showed that the viability of cells was (58 ± 2)% with 800 μmol/L PQ for treatment.The immunofluorescence showed that there were few expressions of H3Cit and MPO in neutrophils in the control group,and there was no NETs formation,which was composed of DNA,H3Cit and MPO.Compared with the control group,a large amount of NETs was generated from neutrophils stimulated by 800 μmol/L of PQ.Meanwhile,quantitative result showed that the content of cell free DNA in the supernatant was significantly increased in PQ poisoning group as compared with that of control group (μg/L:2 235 ± 462 vs.561 ± 87,P < 0.01).The protein expressions of H3Cit and MPO in the supernatant were also significantly increased as compared with those of control group [H3Cit protein expression (gray value):0.23 ± 0.03 vs.0.11 ± 0.01,MPO protein expression (gray value):0.47 ± 0.05 vs.0.21 ± 0.04,both P < 0.05].Conclusion 800 μmol/L of PQ can induce the formation of NETs in human peripheral blood.
9.The design and implementation of DICOM Server Mediate Layer.
Jian-jiang YE ; Jin-yan ZHANG ; Chen-hui ZHAO
Chinese Journal of Medical Instrumentation 2002;26(4):271-274
A DICOM Server Mediate Layer is introduced in this paper. It communicates with modalities according to DICOM3.0 standard on the one hand, provides a simple way to interface with other application on the other hand, this mades the implementation of DICOM service much easier for other applications.
Computer Communication Networks
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instrumentation
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Computer Security
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Computer Systems
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Computers
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Equipment Design
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Radiology Information Systems
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Software
10.Clinical significance of early immunological paralysis in patients with severe H1N1 influenza A
Yongbing QIAN ; Hui XIE ; Rui TIAN ; Jian LU ; Wei JIN ; Ruilan WANG
Chinese Critical Care Medicine 2017;29(7):581-585
Objective To analysis the immunological characteristics of patients with severe H1N1 influenza A, and to provide theoretical basis for predicting the prognosis of the disease. Methods A retrospective analysis was conducted. The clinical data of 15 patients diagnosed with severe H1N1 influenza A and admitted to Shanghai General Hospital of Nanjing Medical University from October 2015 to December 2016 were collected. All the patients were divided into survival and death groups according to 28-day survival. Clinical characteristics, treatment algorithm, organ function, inflammatory reaction and immune cell status were compared, and Cox regression was used to decide the risk factors of 28-day death in patients with severe H1N1 infection A. Results All 15 patients with severe H1N1 infection A were enrolled, most of who presented with cough (93.3%), fever (86.7%), sputum production (80.0%), shortness of breath (73.3%), myalgia (40.0%) and fatigue (40.0%). All had been received anti-virus, antibiotics, mechanical ventilation and anti-coagulation therapy; some were treated with prone position, neuromuscular blocker and extracorporeal membrane oxygenation (ECMO). The incidences of acute myocardial and kidney injury were high, and the acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score (14.1±6.1) and sequential organ failure assessment (SOFA) score (9.6±4.1) implicated the critical condition. Of 15 patients, 4 patients died in 28 days, while 11 were cured and discharged. Compared with survival group, the patients in death group had higher levels of APACHE Ⅱscore (22.7±3.8 vs. 11.8±3.8), troponin [cTn (μg/L): 0.52 (0.07, 2.02) vs. 0.15 (0.10, 0.45)] and blood urea nitrogen [BUN (mmol/L): 11.9 (6.7, 29.1) vs. 3.9 (2.7, 6.8)] and a lower level of blood platelets count [PLT (×109/L): 76±33 vs. 146±49, all P < 0.05]. The levels of C-reactive protein (CRP) and interleukin-6 (IL-6) within 24 hours of admission in death group were significantly higher than those of survival group [CRP (mg/L): 172.2±88.5 vs. 74.8±33.1, IL-6 (ng/L):283.3 (140.1, 711.0) vs. 18.5 (12.7, 71.4), both P < 0.01]. Compared with survival group, the expressions of CD3+, CD4+, CD8+ T cells and natural killer cell (NK cell) in death group were significantly decreased (CD3+ T cell: 0.348±0.119 vs. 0.573±0.106, CD4+ T cell: 0.135±0.046 vs. 0.344±0.098, CD8+ T cell: 0.089±0.057 vs. 0.208±0.054, NK cell: 0.124±0.057 vs. 0.252±0.182, all P < 0.05), but there were no significant differences in CD4+/CD8+ ratio and human leucocyte antigen-DR positive (HLA-DR+) T cell between death group and survival group (CD4+/CD8+ ratio:1.57±0.26 vs. 1.83±0.54, HLA-DR+ T cell: 0.035±0.022 vs. 0.062±0.036, both P > 0.05). B lymphocyte in death group was significantly higher than that of survival group (0.477±0.136 vs. 0.229±0.121, P < 0.01). Cox regression analysis revealed that APACHE Ⅱ score [risk ratio (RR) = 20.4, 95% confidence interval (95%CI) = 5.3-31.2, P = 0.017], CD4+ T cell (RR = 11.1, 95%CI = 5.1-20.0, P = 0.048) and CD8+ T cell (RR = 9.1, 95%CI = 4.3-16.7, P = 0.049) were independently risk factors of 28-day survival of patients with severe H1N1 influenza A. Conclusion Immunological paralysis and severe inflammatory response were early complicated with severe H1N1 influenza A, and these were significantly associated with prognosis.