1.The pilot study on mutations of HIV-1 envelop V3 loop in AIDS patients in Shenzhen
Chinese Journal of Infectious Diseases 2001;0(05):-
Objective To investigate the characteristics of human immunodeficiency virus(HIV-1)V3 loop amino acid mutations among AIDS patients in Shenzhen. Methods Fragments of HIV-1 env gene were amplified by RT-nested-polymerase chain reaction(PCR)from plasma of the AIDS patients in Shenzhen.The PCR products were then directly sequenced and the sequences were aligned,translated and analyzed. Results Sequence analysis showed that there were B and CRF01-AE HIV-1 subtypes in Shenzhen.The V3 loop terra peptide GPGQ accounted for about 48%,GPGR about 36%,other V3 loop terra peptide forms were 16%;the V3 loop central motif in the majority(76.9%)of the CRF01-AE strains was GPGQ,the majority(75%)of the B strains was GPGR.We also found some unusual terra peptide compositions:DQDR and DQGQ on the tip of V3 loop,some amino acid mutations at specific of V3 loop associated with SI phenotype were very common in AIDS patients(80%). Conclusions The V3 loop amino acid mutations were very common in AIDS patients,the V3 loop central motif in the majority was GPGQ and GPGR in Shenzhen,the mutations shows the change of HIV-1 phenotype and predicts that disease progress to AIDS.
2.Construction of a general piggyBac transposon inducible cell immortalization vector and verification of its basic properties.
Hui HUANG ; Guangdong HU ; Jian KANG ; Suzhu QING ; Yong ZHANG
Chinese Journal of Biotechnology 2014;30(8):1182-1192
In order to construct generally efficient cell immortalization vector, pTP-hTERT, we modified the traditional piggyBac (PB) transposon using artificial synthesis, PCR and enzyme digestion. The modified vector contained the necessary transposon elements, a PB transposase expression cassette, a co-expression selectable element and a human telomerase reverse transcriptase (hTERT) expression cassette. The co-expression selectable element had two markers, enhanced green fluorescent protein (EGFP) gene and puromycin-resistance (Puro) gene, linked by porcine teschovirus-1 2A peptide (P2A). To validate the functionality of vector elements, we transfected pTP-hTERT into HEK293 cell, selected the positive cell clones and then conducted RT-PCR, Western blotting (WB) and Tail-PCR, methylene blue staining and statistic analysis on selected cells. The results of sequencing and cell culture show that the pTP-hTERT was constructed successfully and the positive cell could be selected by puromycin. The WB results, P2A cutting EGFP and Puro fusion protein with high efficiency, reflected the selectable element worked. The sequencing result of Tail-PCR confirmed the vector integrated into the genome through transposition. The results of methylene blue staining and statistic analysis indicated the clone of positive cells triggered by pTP-hTERT significantly increased (P < 0.01) compared with control group. The construction of pTP-hTERT provides an efficient tool for establishing immortalized cell lines and a demonstration for building other eukaryotic plasmids.
Cell Line
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DNA Transposable Elements
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genetics
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Genetic Vectors
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Green Fluorescent Proteins
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genetics
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HEK293 Cells
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Humans
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Plasmids
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Polymerase Chain Reaction
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Telomerase
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genetics
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Transfection
3.Construction of sharing type network resource data base for clinical skills teaching
Ailing HUI ; Dong HU ; Jian CHEN ; Lijun TANG ; Jinchun SI
Chinese Journal of Medical Education Research 2012;11(5):525-528
The construction of a network resource data base for clinical skill teaching aiming at sharing excellent teaching resources was completed by integrating all kinds of teaching resource including characters,eourseware,pictures,cartoons,videos and examination questions,etc.In view of the generally weak situation of the current domestic education resource data base construction,the contents,guiding ideology,principle,object,orientation,function and the management mechanism of the network resource data base construction were devised for clinical skills teaching.Suggestions for specific ideas and construction problems were proposed to promote the construction and optimization of the network resource database for clinical skills teaching.
4.Bovine jugular venous conduit treated with the polyepoxy compound.
Zhao-jun XU ; Zhong-shi WU ; Tie-hui HU ; Jian-guo HU ; Yao-guang FENG ; Zhong-sha MA ; Hui WANG ; Jian HU ; Ye-rong HU
Journal of Central South University(Medical Sciences) 2006;31(3):355-358
OBJECTIVE:
To determine the feasibility whether the bovine jugular venous conduit (BJVC) can be fixed with polyepoxy compound (PC).
METHODS:
Twenty-four BJVCs were divided into 3 groups and fixed with polyepoxy compound (PC group, n = 8), glutaraldehyde (GA group, n = 8), and unfixed group (Control group, n = 8), respectively. The morphologic and mechanical properties of BJVCs in the 3 groups, including thickness, diameter, moisture content, denaturation temperature, tensile strength, elongation at break, and fixation index were measured. The rat subcutaneous model for the assessment of tissue calcification was used. The calcium content in bovine jugular vein patches and valves was determined by flame atomic absorption spectrophotometer.
RESULTS:
There was no difference in the wall thickness, diameter, and tissue water content between PC and the control group, but significant difference was found between GA and PC groups. The mechanical properties of PC group and GA group were not significantly different, but they were better than those of the control group. GA-fixed BJVC samples showed clear calcification, while PC fixed BJVC were calcified significantly less.
CONCLUSION
PC is an effective and suitable choice for the treatment of BJVC since it can effectively preserve the structure and the anti-reflow function of valves in bovine jugular vein and it has better anti-calcification properties.
Animals
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Biocompatible Materials
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Bioprosthesis
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Blood Vessel Prosthesis
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Cattle
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Cross-Linking Reagents
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pharmacology
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Epoxy Compounds
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pharmacology
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Jugular Veins
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Polymers
5.Clinical analysis of death cases in pediatric intensive care unit
Hui ZHANG ; Ying WANG ; Biru LI ; Juan QIAN ; Xiaowei HU ; Hong REN ; Jian ZHANG
Journal of Clinical Pediatrics 2014;(7):664-667
Objectives To analysis the main characteristics and changes of the internal death in pediatric intensive care unit (PICU) in the past five years. Methods The clinical data of 330 death cases in PICU internal medicine were retrospectively analyzed from January 2008 to December 2012. Results The mortality rate in PICU dropped from 5.85%to 3.96% from 2008 to 2012. Among 330 death cases, 136 cases (41.2%) were infants, 73 cases (22.1%) were toddlers, 51 cases (15.45%) were preschoolers, and 70 cases (21.21%) were school-age and adolescence. In different years, the differences of distribution of death in different age groups were statistical significance (χ2=6.90, P=0.009). In all years, the infant had the highest death rate. As the time progresses, the death rate of the infant and young children decreases, while the death rate of the school-age and adolescence increases. Among the diseases caused death, the cardiovascular disease was the most common disease (33.94%), followed by hematologic malignancy (31.52%). The difference of distribution of the diseases caused death in different age group was statistically significant (P<0.01). The cardiovascular disease was main cause of death in infant, and the hematologic malignancy was the main cause of death in other three age groups. Within 24 h admission, the pediatric critical illness score (PCIS) had been assessed, 67.49% was critical and 15.48%was extremely critical. The hospitalization time was negatively correlated with PCIS (r=-0.313, P<0.001). Conclusions In the past five years, the mortality in PICU declined year by year. Cardiovascular disease in infancy and hematologic malignancy in non infancy are the leading cause of death in children. Admission in critical or extremely critical condition is the reason of early death in hospital.
6.Effects of Lonicera Japonica flavone on immunomodulation in mice.
Jian-hui PI ; Juan TAN ; Zhao-tun HU ; De-biao XIANG
Chinese Journal of Applied Physiology 2015;31(1):89-92
OBJECTIVETo study immunomodulating activity of Lonicera Japonica flavone by investigating immune enzymatic activity of serum and antoxidized activity of lymphoid organs in mice.
METHODSFifty KM mice were randomly divided into control group, model group, low dose group, middle dose group and high dose group(n = 10), respectively. And low dose group, middle dose group and high dose group were given Lonicera Japonica flavone with 100 mg/kg, 200 mg/kg and 400 mg/kg every day, respectively, while control group and model group were administered with NS. After continuously giving drug 7 weeks, other groups were injected with Dexamethasome (Dex: 25 mg /kg) for 3 days by subcutaneous injection, but the control group were treated with NS. And after giving Lonicera Japonica flavone 1 week simultaneously, organ indexes , the activity of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LSZ) in serum , and the content of monoamine oxidase (MAO), total antioxidant capacity (T-AOC), total superoxide dismutase (SOD) and malondialdehyde (MDA) in lymphoid organs in mice were tested, respectively.
RESULTSLonicera Japonica flavone could significantly improve the organ indexes, and significantly improve the activity of ACP, AKP and LSZ in serum, and significantly improve the contents of T-AOC and SOD, but reduce that of MAO and MDA in lymphoid organs in immunosuppressed mice.
CONCLUSIONIonicera Japonica flavone can significantly improve the activity of immune enzyme in serum and the antioxidized activity of lymphoid organs in mice. It suggests that Ionicera Japonica flavone has a good immunomodulatory effects.
Acid Phosphatase ; blood ; Alkaline Phosphatase ; blood ; Animals ; Antioxidants ; metabolism ; Flavones ; pharmacology ; Immunomodulation ; Lonicera ; chemistry ; Malondialdehyde ; metabolism ; Mice ; Monoamine Oxidase ; metabolism ; Muramidase ; blood ; Superoxide Dismutase ; metabolism
7.Design of a controlling switch set based on RS-232.
Jian-hu HE ; Qing-li ZHOU ; Hui TIAN
Chinese Journal of Medical Instrumentation 2008;32(5):384-387
As a common computer keyboard and a mouse can't meet the requirements of some special occasions, this paper introduces the design of a controlling switch set based on RS-232 for capturing the image at the medical image workstation. Its working principles, circuit, assembly together with its associated software controller are given in detail. The control switch set features easy manufacturing, low cost and a good reliability.
Equipment Design
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Image Processing, Computer-Assisted
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instrumentation
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methods
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Software Design
8.A1 H magnetic resonance spectroscopy study of obsessive-compulsive disorder
Xiuzhi ZHAO ; Xibin HU ; Hui WANG ; Jian QIAO ; Zhonghua SU ; Zhiyin YANG
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(1):28-31
Objective To approach potential abnormalities of neuro-chemical compounds in cerebrum of obsessive-compulsive disorder and to analyze the relationship among the abnormalities with duration of illness and symptom severity. Methods 19 patients with obsessive-compulsive disorder and 22 comparison subjects were scanned by 1H magnetic resonance spectroscopy to exam bilateral prefrontal lobe, temporal lobe, parietal lobe, occipital lobe, anterior cingutate and caudate nucleus, hippocampus as well as splenium and genu of corpus callosum,thalamus. The ratios of N-acetylaspartate (NAA) and choline (Cho) to creatine (Cr) were respectively recorded.The severities of obsessive-compulsive symptoms were assessed by Yale-Brown Obsessive-Compulsive Scale. Results ( 1 ) The ratios of NAA/Cr in right caudate nucleus and left hippocampus were higher than those in normal controls (1.23 ±0.37 vs 1.0 ±0.33, t=2.14, P=0.039;1.34 ±0.25 vs 1. 10 ±0.45, t=2.41, P=0.021 ), but NAA/Cr ratios in genu of corpus callosum were lower(0. 97 ±0.30 vs 1.19 ± 0. 26, t = -2.47, P= 0.018 ).(2) The Cho/Cr ratios in right caudate nucleus and left temporal lobe were higher than those in control groups (1.09±0.51 vs0. 67±0.21,t=3.32, P=0.003;2.58±0.62vs0.84±0.17, t=2.21,P=0.03). (3) The duration of illness was negatively correlated with the Cho/Cr ratios of right prefrontal lobe ( r = - 0. 507, P =0. 027 ). (4) The NAA/Cr ratios of genu of corpus callosum as well as the Cho/Cr ratios of left temporal lobe were negatively correlated with YBOCS score ( r 1 = - 0.457, P = 0.049; r 2 = - 0. 585, P = 0.009 ). Conclusion Neuronal functions increase in right caudate nucleus and left hippocampus which are opposite in genu of corpus callosum. These abnormalities may play an important role in pathogenesis of OCD.
9.Cerebral sparganosis: clinical and radiological features of four cases
Jian BAO ; Hui WANG ; Aimin WU ; Zhuang KANG ; Zhengqi LU ; Ying GUO ; Xueqiang HU
Chinese Journal of Neurology 2010;43(12):869-873
Objective To discuss the diagnosis and treatment of cerebral sparganosis. Methods To summary four cases of cerebral sparganosis, focusing on the clinical course and imaging findings, with the goal of better diagnostic skills. Results All 4 cases had some kind of misdiagnosis and improvement after surgery or parasiticidal pharmacotherapy. Cerebral MRI and CT scans revealed relatively extensive white matter degeneration and focal enhancements. Subsequent scans showed changes in shape and location of the enhanced foci, indicating the migration of sparganum. Pathologic findings of 3 patients who had undergone surgery showed granuloma and sparganum. Conclusions Cerebral sparganosis has relatively special manifestions on imaging, which are of diagnostic value. The spaganum should be as completely removed as possible during surgery.
10.Correlation between expression of aquaporin-4 and the apparent diffusion coefficient on diffusion-weighted imaging after focal cerebral ischemia/reperfusion in rats
Hong LU ; Shizheng ZHANG ; Hui HU ; Jian HE ; Feng ZHAO ; Tao WU
International Journal of Cerebrovascular Diseases 2009;17(3):166-170
Objective To investigate the correlation between the apparent diffusion coefficient (ADC) and the expression of aquaporin-4 (AQP4) in brain tissue after ischemia/reperfusion in rats. Methods A model of right middle cerebral artery occlusion (MCAO) induced by suture method. Seventy Wistar rats were randomly divided into 7 groups: sham-operation (A), MCAO 30 min (B), MCAO 30 min and reperfusion 30 min (D), MCAO 30 minand reperfusion 60 min (E), MCAO 60 min (C); MCAO 60 min and reperfusion 30 min (F), and MCAO 60 min and reperfusion 60 min (G) groups (n=10 in each group). The rats in all groups underwent diffusion-weighted imaging (DWI). The relative apparent diffusion coefficient (rADC) was calculated. Triphenyltetrazolium chloride (TTC) staining was used to detect the ratio of ischemic area. Immunohistochemistry, in site hybridization, and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expressions of AQP4. Results No abnormal intensity signals were observed on DWI in sham-operation group. The ranges of the high signal intensity lesions on diffusion-weighted imaging (DWI) were from small to large in groups B, C, D, E, F, and G. Then it reduced gradually, so did the ischemic area. The AQP4 expression was up-regulated significantly in groups B and E. The AQP4 expression was decreased significantly after the reperfusion in all groups. 1here was significant difference between ischemic groups and reperfusion groups (all P<0. 05). The expressions of rADC and AQP4 showed negative correlation (r=0. 72, P<0.01). Conclusions The AQP4 expression in brain tissue is closely associated with the changes of ADC after cerebral ischemia/reperfusion. DWI may indirectly reflect the levels of AQP4 expression.