Adenocarcinoma ; diagnosis ; metabolism ; pathology ; Carcinoma, Signet Ring Cell ; diagnosis ; metabolism ; pathology ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Neoplasm, Residual ; metabolism ; Prostatic Hyperplasia ; metabolism ; Prostatic Intraepithelial Neoplasia ; metabolism ; Prostatic Neoplasms ; diagnosis ; metabolism ; pathology ; Racemases and Epimerases ; metabolism ; Sensitivity and Specificity

Objective To evaluate the effect of three diamension-digital subtraction angiography (3D-DSA) or computed tomography angiography (CTA) on the patients with ruptured multiple intmcranial aneurysm (MIA). Methods A retrospective study on 21 patients with MIA was performed.After scanning with 3D-DSA or 3D-CTA, three-dimensional reconstruction of MIA was carried out by 3D workstation,then the diagnosis was decided and the treatment plan (endovascular treatment or microsurgery) was selected according to stereoscopic image of MIA. Results (1) 3D-DSA or CTA was performed in 21 patients with subarachnoid hemorrhage (SAH),it was revealed these patients carried with 48 aneurysms,including 35 small aneurysms (25 mm).Not only miero-aneurysms and small aneurysms could be precisely showed,also the size of aneurysmal neck,the relationship of the aneurysm and the parent vessel and contiguous branches by stereoscopic image.(2) According to the standard of classification,9 patients with MIA for gradeⅠ(42.9%),10 for gradeⅡ(47.6%),2 for gradeⅢ(9.5%),0 for gradeⅣ.Endovascular treatment was selected prior to microsargery for those high grade patients.In this group,17 patients with 40 aneurysms underwent endovascular embolotherapy with GDC coils.Twenty four anemysms were completely occlusioned,12 beyond 90%,4 were left without treatment because of their small size.In microsurgery group,3 aneurysrus were totally clipped,1 could not be found during operation.No any treatment was accepted in 2 patients with 4 aneurysms. Conclusions 3D-DSA or CTA,which is very useful for the diagnosis and treatment of MIA,could improve the accuracy of diagnosis of MIA and clearly show the stereoscopic image of MIA,also the relation of sac and parent artery.For those patients with high grade MIA,endovascular treatment was selected prior to microsurgery,pro re nata,used to combine with mierosurgery.

Three new compounds, including a naphthoquinone, a reduced naphthoquinone derivative naphthalenone, and a tricarboxylic acid, along with five known naphthalenone derivatives were isolated from ethyl acetate extract of rice fermentation products of the fungus Pleosporales sp. by multiple column chromatographic methods, including Sephadex LH-20 gel column chromatography, silica gel column chromatography, reversed-phase HPLC, and chiral chromatography. Their structures were elucidated by MS, NMR, and specific rotation spectroscopic analyses as well as ECD calculations. Three novel compounds were named as pleospathone A (1), pleospathone B (2), and pleosporalic acid A (3). Five known compounds were separately identified as (3S,4R)-3,4,8-trihydroxy-6-methyl-3,4-dihydronaphthalen-1(2H)-one (4), (4R)-3,4-dihydro-4,6,8-trihydroxy-1(2H)-naphthalenone (5), (-)-scytalone (6), (3S,4S)-3,4-dihydro-3,4,6,8-tetrahydroxy-1(2H)-naphthalenone (7), and cis-4-hydroxyscytalone (8). Compounds 4-8 were isolated from the Pleosporales fungi for the first time. Compound 1 shows inhibitory activities against human cervical carcinoma cell line HeLa and murine leukemia cell line P388 with the IC₅₀ values of 78.93 and 98.80 μmol·L^-1, respectively.

Objective To investigate the value and dynamic characteristics of diffusion-weighted imaging(DWI)in rabbit liver VX_2 tumor model,with correlation of pathology.Methods Forty New Zealand rabbits were included in the study and VX_2 tumor piece was implanted directly into the liver after laporotomy.Fiffteen had two intrahepatic implants while twenty-five had one implant.DWI was performed on the seventh,fourteenth and twenty-first day after implantation,while routine T_1WI and T_2WI sequences were done on the seventh and fourteenth day.Ten VX_2 tumor samples were studied by pathology.Results The lump detection rates on the seventh day after implantation of DWI,T_1WI and T_2WI were 78.7%(37/47), 10.7%(3/28)and 53.6%(15/28)respectively with statistical significance(x~2=32.61,P

0.05).Conclusions The potential of VX_2 tumor infiltration and metastasis will decrease after chemoembolization in the long-run,while the proliferation ability will increase within a short period of time. The ADC may reflect the proliferation of VX_2 tumor cells.

OBJECTIVETo investigate the preventive effect of Jiangu granule (JGG) on experimental primary osteoporosis type I.

METHODSOsteoporosis model was established through ovary resection of female rats. Bone mineral density (BMD) was measured with double energy X-ray absorptiometry. Level of endocrine markers, including osteocalcin (BGP), estradiol (E2) and calcitonin (CT) in serum were examined by RIA. And uterus parameters was calculated also.

RESULTSJGG could significantly increase BMD and uterus index, improve the levels of E2 and CT in serum, at the same time reduce the BGP level.

CONCLUSIONJGG can efficiently prevent type I primary osteoporosis or delay its occurrence by enhancing the function of endocrine system, coordinating the action of calcium related hormone, reducing bone turnover rate and increasing BMD.

Animals ; Bone Density ; Calcitonin ; blood ; Drugs, Chinese Herbal ; pharmacology ; Estradiol ; blood ; Female ; Osteocalcin ; blood ; Osteoporosis ; blood ; prevention & control ; Ovariectomy ; Phytotherapy ; Random Allocation ; Rats ; Rats, Sprague-Dawley

Adult ; Anemia, Aplastic ; complications ; Antiphospholipid Syndrome ; complications ; Humans ; Male ; Sinus Thrombosis, Intracranial ; etiology

OBJECTIVETo investigate the chemical constituents from Hedyotis diffusa.

METHODThe compounds were isolated and purified by various chromatographic techniques and identified by their physicochemical properties and spectral data.

RESULTEight compounds were isolated and identified as octadecyl (E)-p-coumarate (1), p-E-methoxy-cinnamic acid (2), ferulic acid (3), scopoletin (4), succinic acid (5), aurantiamide acetate (6), rubiadin (7), robustaquinone D (8).

CONCLUSIONCompounds 1-8 were obtained from genus Hedyotis for the first time.

Anthraquinones ; chemistry ; isolation & purification ; Coumaric Acids ; chemistry ; isolation & purification ; Dipeptides ; chemistry ; isolation & purification ; Hedyotis ; chemistry ; Plant Extracts ; chemistry ; isolation & purification ; Scopoletin ; chemistry ; isolation & purification ; Succinic Acid ; chemistry ; isolation & purification

Background and objectiveMaintenance of genomic integrity is essential to ensure normal organismal development and to prevent diseases such as cancer. PR-Set7 (also known as Set8) is a cell cycle regulated enzyme that catalyses monomethylation of histone 4 at Lys20 (H4K20me1) to promote chromosome condensation and prevent DNA damage. Recent studies show that CRL4CDT2-mediated ubiquitylation of PR-Set7 leads to its degradation during S phase and atfer DNA damage. hTis might occur to ensure appropriate changes in chromosome structure during the cell cycle or to preserve genome integrity atfer DNA damage.Methods We developed a new model of lung tumor development in mice harboring a conditionally expressed allele of Cul4A. We have therefore used a mouse model to demonstrate for the ifrst time that Cul4A is oncogenicin vivo. With this model, staining of PR-Set7 in the preneoplastic and tumor lesions in AdenoCre-induced mouse lungs was performed. Meanwhile we identiifed higher protein level changes of γ-tubulin and pericentrin by IHC.Results hTe level of PR-Set7 down-regulated in the preneoplastic and adenocarcinomous lesions following over-expression of Cul4A. We also identiifed higher levels of the proteins pericentrin and γ-tubulin in Cul4A mouse lungs induced by AdenoCre.Conclusion PR-Set7 is a direct target of Cul4A for degradation and involved in the formation of lung tumors in the conditional Cul4A transgenic mouse model.

Objective To discuss the effect of extended care on quality of life of cervical cancer patients with hysterectomy.Methods A total of 56 cases of cervical cancer patients with hysterectomy were randomly divided into the experimental group and the control group,each with 28 cases.The control group received routine discharge instructions,while the experimental group was given extended nursing in addition.Cancer rehabilitation evaluation system-short form (CARESSF) scale was used to evaluate patients'quality of life respectively 1,3 and 6 months after discharge.Results 1,3 and 6 months after intervention,the scores of biological dimensions were respectively (15.36 ± 1.77),(11.07 ± 2.19),(7.75 ± 1.58),the scores of psychological and social dimensions were (14.32 ± 1.74),(9.79 ± 2.36),(6.71 ± 1.49),and the scores of relationship with medical staff were (4.21 ± 1.10),(4.29 ± 1.08),(2.96 ± 1.14) in the experimental group.1,3 and 6 months after intervention,the scores of biological dimensions were respectively (17.36 ± 1.64),(14.39 ± 1.42),(9.79 ± 2.32),the scores of psychological and social dimensions were (16.21 ± 2.04),(14.07± 1.59),(9.14 ± 1.94),and the scores of relationship with medical staff were (6.71 ± 1.27),(6.86 ± 1.33),(5.79 ± 1.40).Quality of life related to health changed with time in both groups,with statistically significant difference (P ＜0.01).Quality of life was much better in the experimental group than in the control group,and the differences were statistically significant (t =-4.391,-6.723,-3.845,-3.729,-7.971,-5.260,-7.863,-7.947,-8.285,respectively; P ＜0.05).Conclusions Extended care can improve the quality of life of cervical cancer patients with hysterectomy,and help to develop healthy mental status and establish health behaviors.