1. In vitro effects of propofol on apoptosis and Bax expression induced by TNF-α in mouse spinal cord neurons
Academic Journal of Second Military Medical University 2006;27(2):169-172
Objective: To study the in vitro effects of propofol on apoptosis and Bax expression induced by TNF-α in mouse spinal cord neurons. Methods: Spinal cord neurons were isolated from fetal mice and cultured in neurobasal medium with B27 supplement. On the 7th day of culture, neurons were randomly divided into 6 groups: control group, propofol (50 μmol/L) group, TNF-α group, propofol(25 μmol/L) with TNF-α group, propofol(50 μmol/L) with TNF-α group and propofol(100 μmol/L) with TNF-α group. Propofol with different concentrations was incubated with cultured cells for 30 min, then TNF-α was added with the final concentration of 2 000 U/ml for another 24 h incubation. Apoptosis was detected by PI/Hoechst33342 double staining technique and fluorescence microscopy. Bax expression was determined by immunocytochemical technique. Results: The apoptosis rate and expression of Bax in TNF-α group were both increased compared with those in control group ([21.8±1.1]% vs [2.8±0.8]%,P<0.01; [0.251±0.016] vs [0.141±0.015],P<0.01). The apoptosis rates and expression of Bax in 3 propofol groups(25, 50, 100 μmol/L) were ([16.2±1.2]%, [15.3±0.6]% and [12.2±0.8]%) and ([0.198±0.011],[0.188±0.012] and [0.167±0.014]), respectively, and they were all significantly decreased compared with those in TNF-α group ([21.8±1.1]% and [0.251±0.0163], all P<0.05). Moreover, a dose-dependent manner was observed in the above changes. Conclusion: Propofol within clinical concentration ranges may inhibit the apoptosis of spinal cord neurons induced by TNF-α through modulating the expression of Bax.
2.Effects of exogenous FHIT gene on proliferation and apoptosis of human glioma cell line U87
yang-hua, XIA ; jian-kang, SHEN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(08):-
Objective To investigate the effects of exogenous fragile histidine triad(FHIT) gene on apoptosis of human glioma cell line U87. Methods By the method of liposome transfection,plasmids pcDNA3.1/myc-His(-)B-FHIT and pcDNA3.1/myc-His(-)B were transfected into glioma cell line U87.U87 cells were divided into three groups: U87-FHIT group,U87 cells transfected by plasmids pcDNA3.1/myc-His(-)B-FHIT;U87-vector group,U87 cells transfected by plasmids pcDNA3.1/myc-His(-)B;and blank control group,U87 cells without transfection.The expression of exogenous FHIT protein was detected by Western blot and immunofluorescence staining.The effects of FHIT on the growth characteristics of U87 were observed by MTT and flow cytometry. Results Growth inhibitory rate and apoptosis rate of the cells in U87-FHIT group were significantly higher than those in U87-vector group and blank control group(P
3.Research on tumor suppressor gene FHIT
yang-hua, XIA ; jian-kang, SHEN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(02):-
Fragile histidine triad(FHIT) gene is a tumor suppressor gene that locates on chromosome 3p14.2.FHIT can induce cell apoptosis and inhibit cell growth by activating caspase,inhibiting PI3K-Akt-survivin signal pathway and phosphorylation of I?B-?,and binding with microtube.The inactivation of FHIT is closely related with carcinogenesis.The advances in research on the structure,biological function,relationship between inactivation and carcinogenesis,and gene therapy of FHIT are reviewed in this paper.
4.The Pathogenesis of Cerebral Arteriovenous Malformations
Yang-Hua XIA ; Jian-Kang SHEN ;
International Journal of Cerebrovascular Diseases 2006;0(10):-
Cerebral arteriovenous malformation is a common cerebrovascular disease.Its exact pathogenesis remains unclear.At present,it is thought that this disease is caused by kinds of factors,including congenital and acquired factors.
5.Combination of prenatal sonography and MRI in diagnosis of fetal urology reproductive system abnormalities
Jian-hua, LI ; Xia, ZHU ; Xin-lin, CHEN
Chinese Journal of Medical Ultrasound (Electronic Edition) 2010;07(12):2156-2160
Objective To compare the diagnostic value of prenatal ultrasound with magnetic resonance imaging(MRI)in the abnormalities of fetal urinary reproductive system,and explore the value of combining prenatal ultrasound and MRI in diagnosis of fetal urinary and reproductive system abnormalities.MethodsTwenty-seven fetal abnormalities in urinary reproductive system detected by prenatal ultrasound received MRI examination within 24 hours after ultrasound,confirmed by autopsy or clinical follow-up.The results of prenatal ultrasound and MRI were analyzed retrospectively.Results Ultrasound,MRI,autopsy or after birth,postpartum follow-up confirmed 27 cases of Genitourinary system anomalies,including 2 cases of hydronephrosis,renal agenesis in 6 cases,14 cases of renal cystic disease,renal fusion in 3 cases,1 case of renal tumors,ovarian cyst in one case of crown .Fetal ultrasound can diagnose the majority of the genitourinary system anomalies (25/27),MRI can diagnose fetal anomalies of 26 cases of genitourinary system.Conclusion Ultrasound could diagnose the majority of fetal abnormalities in urology reproductive system accurately.However,combining ultrasound and MRI could establish more precise estimation in urology reproductive system,such as the level of obstruction of urinary system in hydronephrosis,the pulmonary hypoplasia secondary to urology system abnormalities,and location of cyst in the renal parenchyma or in the renal capsule.
6.Drug Sensitive Analysis of 207 Strains of Staphylococcus Aureus in Children
min, XU ; jian-hua, ZHANG ; liang-xia, WU ; zi-cai, WANG
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To analyze the changes of the frequency of staphylococcus aureus (SAU) and methicillin resistant staphylococcus aureus (MRSA) isolated from hospitalized children from 2003 to 2006,and investigate the 21 antimicrobial susceptibility against SAU strains for providing reference for clinical rational use of drug.Methods The SAU strains were isolated from 207 children who were hospitalized in department of pediatrics of the sixth people′s hospital affiliated to Shanghai jiaotong university from Jan.2003 to Dec.2006 during the first 48 h of hospital stay.Strains were isolated from cotton-tipped swab of throat culture 75 strains,sputum culture 57 strains,secretion of bellybutton culture 25 strains,liquor puris culture 23 strains,hemoculture 20 strains,tube thoracostomy culture 3 strains,middle piece urnary culture 3 strains,cerebrospinal fluid culture 1 strain.The strains were identified by latex agglutination test,the in vitro activities of the 21 antimicrobial agents against 207 SAU isolates was tested by disc diffusion test (K-B method) according to the guidelines of the clinical and laboratory standards institute.MRSA was screened by oxacillin disc diffusion test.The data were analyzed by WHONET 5 software,Cochran-Mantel-Haenszel was used to analyze the tendency of SAU isolating rate and MRSA detectable rate.Results Of these SAU,30 strains(14.5 %) isolates were MRSA and 177 strains(85.5 %) were methicillin sensitive staphylococcus aureus.The sensitive rates of SAU to Penicilin,Oxacillin,Cefuroxime Sodium,Cefotaxime,Ceftriaxone,Erythromycin,Clindamycin,Trimethoprim/Sulfamethoxazole,Ciprofloxacin,Fosfomycin and Rifampin were 4.8%,85.5%,84.9%,90.0%,88.3%,37.5%,46.3%,92.7%,74.2%,97.2%,94.0%,respectively.All the strains were sensitive to peptide antibiotic(for instance,vacomycin and teicoplanin) and Levofloxacin.The SAU isolated rate and MRSA detectable rate were 9.6%,19.8%,25.0%,39.5% and 5.0%,5.9%,12.7%,24.7% in 2003-2006,respectively.Conclusions SAU has strong resis-tance to Penicilin and Erythromycin,but relative sensitive to Oxacillin and Cephalosporin.The SAU isolatating rate and MRSA resistant rate is increased in our area,pediatrics should to strengthen the monitoring of SAU is important in pediatrics.
7.Effects of Family Allergic History and Dermatophagoides Farina on Expression of Th1/Th2 Cytokine of Cord Blood and Allergic Disorders in Infancy
jian-hua, ZHANG ; jun, SHI ; liang-xia, WU ; zi-cai, WANG
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To investigate the effects of family allergic history and dermatophagoides farina on the expressions of Th1/Th2 cytokine of cord blood and allergic disorders in infancy.Methods Ten mil cord blood were obtained from 34 neonates which 17 cases had family allergic history and 17 cases didn′t have.Cord blood mononuclear cells(CBMC) were isolated by gradient centrifugation with Ficoll and were cultured with phytohemagglutinin(PHA) or dermatophagoides farina for 48 hours in vitro.The expressions of interleukin(IL-4) and interferon(IFN-?) of the culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA).Two groups were visited with telephone or clinical service every 1 or 2 months in 1 year follow-up survey.Results In no stimulation,the expressions of IL-4 of family allergic history and no family allergic history were(11.35?1.80) ng/L and(11.0?1.50) ng/L,respectively,there was no significant difference.The expressions of IFN-? were(9.55?1.47) ng/L and(10.19?1.37) ng/L,respectively,there was no significant difference also.In PHA stimulation,the expressions of IL-4 were(43.45?4.57) ng/L and(37.58?3.41) ng/L,respectively,there was significant difference.The expressions of IFN-? were(72.61?25.40) ng/L and(65.84?29.96) ng/L,respectively,there was no significant diffe-rence.In low density dermatophagoides farina stimulation,the expressions of IL-4 were(40.54?3.64) ng/L and(37.17?2.60) ng/L,respectively,which had significant difference.The expressions of IFN-? were(35.30?2.73) ng/L and(40.55?1.85) ng/L,respectively,which had significant difference.In high density dermatophagoides farina stimulation,the expressions of IL-4 were(43.50?3.19) ng/L and(39.55?4.13) ng/L,respectively,which had significant difference.The expressions of IFN-? were(39.40 ?5.21) ng/L and(40.94?2.96) ng/L respectively,which had no significant difference.Allergic diseases were happened in 7 cases of 13 cases with family allergic history and in 2 cases of 15 cases without family allergic history in 1 year follow-up except lost follow-up cases.There were significant difference in 2 groups.Conclusions Th2 cells of cord blood are relative dominant in neonates having family allergic history.Th2 cells relative dominant are more obvious in dermatophagoides farina stimulation.The neonates having family allergic history have a tendency to get allergic diseases in childhood.
8.Expressions of Chemokine Receptor CXCR3 and Its Ligand Interferon-?-Inducible Protein-10 in Lung Tissue of Asthmatic Model Mice and Their Significances
liang-xia, WU ; min, WU ; xiao-liang, LIN ; jian-hua, ZHANG
Journal of Applied Clinical Pediatrics 2006;0(16):-
Objective To observe the expressions of the chemokine receptor CXCR3 and its ligand IFN-?-inducible protein-10(IP-10) in lung tissue of asthmatic model mice and to explore further the effect of dexamethasone(DEX)and bacillus Calmette-Guerin vaccine(BCG)on the expressions of CXCR3 and IP-10.Methods Forty Kunming mice were randomly divided into 4 groups:asthmatic group,DEX group,BCG group and control group,10 in each group.Mice were sensitized and challenged with ovalbumin (OVA) to establish asthmatic models.Ten mice in DEX group were administrated DEX (2 mg/kg) by abdomen injection 30 min before challenge (DEX group).Ten mice in BCG group were injected BCG(0.025 mg) intradermally 7,3,and 1 days before sensitization.The mice in control group mice were treated with 9 g/L saline instead of OVA.Mice of each group were executed 24 hours after the final challenge.Their lung tissue were paraffin embedded,sliced and stained by HE.The expressions of CXCR3 and IP-10 protein in lung tissue of mice were detected by immunohistochemistry.Results Distinct differences were discovered in the expressions of CXCR3(F=4.602 P=0.008) and IP-10(F=4.207 P=0.012) among the 4 groups.The expressions of CXCR3 and IP-10 in lung tissue of mice in asthmatic group were significantly higher than those in control group (Pa=0.002),while that in DEX group were significantly lower than that in asthmatic group (P=0.029,0.019).There were no significant difference between the BCG group and asthmatic group.Conclusions The chemokine receptor CXCR3 and its ligand IP-10 play an important roles in mechanisms in the pathogenesis of asthma.DEX and BCG can interfere the expressions of CXCR3 and IP-10 in varying degress.
9.Significance of Determination of Circulating Endothelial Cells in Children with Henoch-Schonlein Purpura
xi-xia, GUO ; hua, WANG ; jian-jiang, ZHANG ; li-ping, ZHENG
Journal of Applied Clinical Pediatrics 2006;0(21):-
Objective To explore the importance effect of circulating endothelial cells(CEC)in the pathogenesis of Henoch-Schonlein purpura(HSP)by determining the level of CEC in children with HSP,and to explore the relationship between CEC and therapeutic effect of HSP and the relationship between CEC and Henoch-Schonlein purpura nephritis(HSPN).Methods Sixty-six inpatients with HSP and 30 children as healthy control group were selected in the First Affiliated Hospital of Zhengzhou University from May to Dec.2008.The blood of all children,including the children in healthy control group,were sampled 1 mL in the early morning.And the CEC of all children were measured by flow cytometry method.The variation of CEC was analyzed in patients with HSP at acute stage and in healthy control group,in patients with HSP and in patients with HSPN,and in patients with HSP without renal damage at recovery stage and in patients with HSPN at recovery stage.SPSS 13.0 statistical software was used to analyze the data.Results CEC in patients with HSP at acute stage[(47.934 5?16.902 6)%]was significantly higher than that in healthy control group[(37.436 7?10.200 7)%](t=2.900 P0.05).CEC in patients with HSPN at recovery stage[(50.258 8?12.824 2)%]was significantly higher than that in patients with HSP without renal damage at recovery stage [(40.864 0?8.165 2)%](t=2.906 P
10.The relationship between liver fat content and liver disease outcome in patients with type 2 diabetes
Linshan ZHANG ; Hua BIAN ; Hongmei YAN ; Mingfeng XIA ; Xinxia CHANG ; Jian GAO ; Xin GAO
Chinese Journal of Endocrinology and Metabolism 2014;30(1):3-7
Objective To investigate the effects of diabetic duration on liver fat content (LFC) in patients with type 2 diabetes,and to explore its relationship with the outcome of liver disease.Methods A total of 435hospitalized patients with type 2 diabetes were recruited.The history data,results of laboratory tests,and hepatic 1 H-MRS were collected,and nonalcoholic fatty liver disease (NAFLD) fibrosis score (NFS) was calculated.Results The prevalence of NAFLD in newly-diagnosed type 2 diabetes mellitus (NT2DM) group was higher than that in predousb-diagnosed type 2 diabetes mellitus (PT2DM) group (92.7% vs 82.2%,P<0.05),with higher LFC [(27.97 ± 16.88 vs 19.44± 15.54) %,P<0.01].The LFC was reduced with prolonged duration of diabetes.Partial correlation analysis showed that LFC was negatively correlated with duration of diabetes (rs =-0.233,P<0.01) after adjustment for gender,age,body mass index (BMI),oral anti-diabetic drugs,lipid-lowering drugs,and insulin treatment.Multiple linear regression analysis showed that LFC was positively correlated with BMI,albumin,and alanine aminotransferase while negatively correlated with duration of diabetes.The proportion of patients without advanced fibrosis (NFS<-1.455) was significantly higher in NT2DM group than that in PT2DM group (26.3% vs 15.5%,P<0.05),and the proportion of PT2DM in patients with advanced fibrosis (NFS>0.676) was significantly higher than that of NT2DM (79.2% vs 20.8%,P<0.05).NFS was positively correlated with the duration of diabetes (rs =0.236,P<0.01).The liver fat content in patients with advanced liver fibrosis decreased significantly,and the LFC was negatively correlated with NFS (rs =-0.164,P<0.01).Conclusions The duration of diabetes is an independent influencing factor of LFC.With the extension of the duration of diabetes,the decreased LFC in type 2diabetic patients with NAFLD is related to the development of advanced fibrosis.The decrease in LFC in type 2diabetic patient is associated with poor outcome of NAFLD.