Child, Preschool ; Fibroma ; pathology ; Humans ; Male ; Oropharyngeal Neoplasms ; pathology

Humans ; Male ; Middle Aged ; Polychondritis, Relapsing ; surgery ; Tracheotomy ; methods

Objective To examine the distribution of flurbiprofen axetil in cerebral-spinal fluid (CSF) by determining the CSF concentration of flurbiprofen after iv administration. Methods Seventy-two ASA Ⅰ or Ⅱ patients of both sexes aged 18-75 yr weighing 54-82 kg undergoing spinal or combined spinal-epidural anesthesia for lower extremity or lower abdominal surgery were studied. Flurbiprofen axetil 1 mg/kg was injected intravenously.CSF 2 ml and venous blood 3 ml were obtained simultaneously every 5 min after iv injection for 45 min (T1-9 ) for determination of flurbiprofen concentration using high performance liquid chromatography, and the CSF/blood flurbiprofen concentration ratio was caculated. Results Flurbiprofen was not detected in CSF at T1,2 after iv injection in 3 and 4 patients. The CSF flurbiprofen concentration was significantly higher at T4-9, and CSF/blood flubiprofen concentration ratio higher at T5-9 than at T3 ( P ＜ 0.05). There was no significant difference in CSF flurbiprofen concentrations among T4-9 ( P ＞ 0.05 ) Conclusion Flurbiprofen is detected in CSF after iv injection, the CSF flurbiprofen concentration peaks at 20 min after iv injection and it lasts until 45 min after iv injection.

BACKGROUND:Bone marrow mesenchymal stem cells(BMSCs)are widely utilized as seed cells or carriers in bone tissue engineering and gene therapy.Thus,how to obtain BMSCs with high purity arose more attentions of researchers.OBJECTIVE:To observe the effects of different separation methods and culture conditions on biological characteristics of rabbit BMSCs.METHODS:BMSCs were obtained by whole bone marrow culture,density grand centrifugal and red blood cell lysis.At 48 hours after culture,the cell numbers were counted,the time of passage was recorded,in addition,the cell morphology was observed by phase contrast microscope,and the CD44 antigen expression was identified using flow cytometry.The 3rd and the 7th generation aging cells were cultured with DMEM-LG,MEM-HG,and DMEM/F12 culture medium.MTT and count cell plat were used to evaluate the growth of BMSCs.Phase contrast microscope was used to observe the morphological changes of aging cells.RESULTS AND CONCLUSION:BMSCs could be separated by each method.The adherent cells showed shuttle or multiple angle shapes,with rich cytoplasm,and positive for CD44 antigen.The more cell number and shortest primary culture time was presented in red blood cell lysis group(P ＜ 0.05).DMEM/F12 could promote the proliferation of quiescent cells.And the cells prevented the better viability.The method of read blood cell lysis improving the efficiency of BMSCs adherent is an effective method of extraction of BMSCs.DMEM/F12 could promote the proliferation Maybe,DMEM/F12 is more suitable for BMSCs.

Objective To study the combined effect of chlorotoluron and atrazine on the testis of mice. Methods Kunming mice were divided into groups and treated with chlorotoluron and atrazine alone or in combination by gavage at different doses for 25 consecutive days. Microscope and electron microscope were used to observe the morphological changes. Results The herbicides used alone or in combination, at all the test doses, caused the morphological changes in degrees in the testis. Under the light microscope, seminiferous epithelium arrayed loosely and disorderedly, spermatogenic cell shed, and layers lessened compared with the control group. Under the electron microscope, mitochondria in the seminiferous epithelium appeared vacuolated, karyotheca swelled, bent and the function of sustentacular cell declined. Compared with alone use groups, the pathological changes were more serious in combination use groups. Conclusion Chlorotoluron and atrazine can produce a combined toxic effect on the testis of mice.

Objective To investigate the potential risk factors for the death of patients with colorectal cancer during perioperative periods.Methods The clinical data of 545 patients with colorectal cancer were analyzed retrospectively.17 factors,which may influence mortality of patients with colorectal cancer during perioperative periods were evaluated.These enumeration data were analyzed with x2 test,then those factors with obvious difference in statistics were further analyzed by multi-factor Logistic regression analysis.The independent risk factors affecting the mortality of patients with colorectal cancer during perioperative periods were obtained from the analysis.Results There were 6 independent risk factors derived that had significant impacts on perioperative death that were postoperative complications,operation method,non-planed reoperation,preoperative electrolyte disturbance,preoperative hypoalbuminemia,and age(P ＜ 0.05).Conclusions Many potential factors could affect perioperative mortality of patients with colorectal cancer undergoing surgical procedure.

Histiocytoma, Benign Fibrous ; Humans ; Male ; Middle Aged ; Orbital Neoplasms

OBJECTIVETo compare differences of therapeutic effect between acupuncture method of tonifying-qi and dredging-meridian and conventional acupuncture method.

METHODSSeventy cases of blepharospasm were randomly divided into an observation group and a control group, 35 cases in each one. Cuanzhu (BL 2), Yuwei (Extra) through Yuyao (EX-HN 4), Xinming 1 (Extra), Sibai (ST 2) and Yintang (GV 29) were selected as main acupoints. The acupuncture method of tonifying-qi and dredging-meridian was applied in the observation group, which was an acupuncture manipulation that could achieve strong needling sensation of qi arrival combined with electric-impulse stimulation. The conventional acupuncture method was applied in the control group. The treatment was given once every other day, 10 times as a session for continuous three sessions in both groups. The severity of blepharospasm was compared before and after treatment in two groups and clinical therapeutic effect was assessed. Follow-up visit was performed after half a year to observe the recurrence rate.

RESULTSThe cured and markedly effective rate was 77.1% (27/35) in the observation group, which was superior to 51.4% (18/35) in the control group (P < 0.05). Among cured and markedly effective patients in both groups, the recurrence rate was 20.0% (4/20) in the observation group, which was inferior to 54.5% (6/11) in the control group (P < 0.05) in the follow-up visit.

CONCLUSIONThe acupuncture method of tonifying-qi and dredging-meridian could effectively improve severity of blepharospasm, which has superior short-term and long-term effects to conventional acupuncture method.

Acupuncture Points ; Acupuncture Therapy ; Aged ; Blepharospasm ; therapy ; Female ; Humans ; Male ; Meridians ; Middle Aged ; Qi ; Treatment Outcome

AlM: To explore the clinical application effect of phacofragmentation combined with vitrectomy in the treatment of traumatic lens dislocation.
METHODS:Totally 16 cases (16 eyes) of traumatic lens dislocation treated with phacofragmentation combined with vitrectomy were retrospectively analyzed, including 6 cases of high intraocular pressure ( lOP) and 10 cases of vitreous hemorrhage, and 1 case of retinal detachment. All patients were given conventional flat line standard three channel vitreous operation incision to remove the anterior, middle part and peripheral vitreous around lens dislocation. The crystalline lens were drawn to the center cavity of vitreous body and treated by ultrasonic disintegrator. ln the operation, the retina was examined and 8 of them had no retinal damage and in the first stage underwent fixation of posterior chamber intraocular lenses.
RESULTS: All crystalline lens dislocated were completely grinded and suctioned. There was no retinal detachment occurred in 3mo followed up. 16 eyes had normal lOP (12-20mmHg) at 1wk after operation. The average visual acuity was improved and with 8 cases got improved of 0. 2 or more 1wk after operation.
CONCLUSlON: Our research shows that phacofragmentation combined with vitrectomy is a safe and effective method for the treatment of traumatic lens dislocation. The patients with nondestructive retina in the operation are feasible to do first stage operation of intraocular lens suture fixation, which contributes to the best visual acuity.

Objective To describe the work of quality control for lipids measurement in CNHS 2002.Methods The quality control was applied over the whole project including staff training,making standard operating procedure(blood collection,separation,temporary storage,transportation,final storage),lab materials assignment,internal quality control in central lab and participating US Disease Control Center's Lipids Standard Program(LSP)as external quality control.Results Seven times of national training programs were held and 326 technicians from CDCs in 31 provinces,autonomous regions, municiplalities and 132 surveyed counties received related trainings.During the program,321 persons passed examination (98.5%)and among them 205 got the A score(62.9%);The field work was implemented strictly according to the procedure. Three automatic biochemical instruments were used in the measurement and there is no significant difference between means of serum TC,TG and HDL-C from several batches of quality control serum.Parallel measurements were conducted in five percent randomly selected samples,the ratio of bias less than 3% was 99.05%,96.40% and 98.30% for TC,TG and HLD-C, respectively.Compared with means of LSP control sera,the results showed that all bias was less than 5% except one bias of TG result(7.02%)in one batch of LSP controls.Conclusion The work of quality control in this survey guaranteed the accuracy of plasma lipids measurement,and provided the basic data for the epidemiological description of dyslipidemia status among Chinese and the further analysis.