1.Expression of CD35 on erythrocyte and the changes of serum inflammatory cytokines in patients with nonalcoholic steatohepatitis and its immune regulation.
Mei-xian HUANG ; Jian-hang LENG ; Qun MIAO ; Yun-feng PAN
Chinese Journal of Hepatology 2004;12(8):503-504
Erythrocytes
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metabolism
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Fatty Liver
;
blood
;
Female
;
Hepatitis
;
blood
;
Humans
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Interleukin-6
;
blood
;
Interleukin-8
;
blood
;
Male
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Middle Aged
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Receptors, Complement 3b
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blood
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Thymosin
;
therapeutic use
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Tumor Necrosis Factor-alpha
;
metabolism
2.Inhibitory effect of ZD6474 combined with adriamycin on MCF-7 human breast cancer cells in vitro.
Jie SHEN ; Ben-fu HE ; Jian RUAN ; Peng ZHAO ; Jing-xia MIAO ; Hang ZHENG ; Rong-cheng LUO
Journal of Southern Medical University 2010;30(3):523-525
OBJECTIVETo investigate the killing effect of ZD6474 combined with adriamycin (ADM) on MCF-7 human breast cancer cells.
METHODSThe inhibitory effects of ZD6474 and ADM alone and in combination on the proliferation of MCF-7 cells were assessed by MTT assay. The cell cycle and cell apoptosis were detected by flow cytometry.
RESULTSZD6474 and ADM both significantly inhibited the proliferation of MCF-7 cells, showing a synergistic effect of their reactions in combined use (P<0.05). ZD6474 or ADM alone caused cell cycle arrest at G0/G1 and S phases, respectively. Combined use of the two drugs resulted in significant reduction of the M-phase cell percentage and cell cycle arrest at G0/G1 and S phases. The coadministration of the drugs significantly increased the apoptosis rate of the cells as compared with ZD6474 or ADM treatment alone (P<0.05).
CONCLUSIONSZD6474 and ADM show a synergistic effect in inhibiting the proliferation and inducing apoptosis of MCF-7 cells.
Antibiotics, Antineoplastic ; pharmacology ; Antineoplastic Agents ; pharmacology ; Apoptosis ; drug effects ; Breast Neoplasms ; pathology ; Cell Cycle ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Doxorubicin ; pharmacology ; Drug Synergism ; Female ; Humans ; Piperidines ; pharmacology ; Quinazolines ; pharmacology
4.Experimental study on Yougui recipe in preventing osteolysis surrounding artificial prosthesis.
Yun-Huo CAI ; Peng ZHAO ; Qiang MAO ; Hang-Xing BAO ; Jin-Fa WANG ; Miao-Feng HU ; Lu-Wei XIAO ; Pei-Jian TONG
China Journal of Orthopaedics and Traumatology 2012;25(12):1010-1015
OBJECTIVETo explore effects of Yougui recipe (see text) and salmon calcitonin acetate in preventing osteolysis surrounding artificial prosthesis.
METHODSThirty-two SD male rats with weighted (250 +/- 20) g, aged 8 weeks, were randomly divided into four groups: blank group, model group, salmon calcitonin acetate group and Yougui recipe (see text) group, and 8 rats in each group. Blank group did not undergo any process, other 24 rats underwent anesthesia by chloral hydrate, their knee joints were exposed through medial patellar side,drilling from fermoral condyle nest to marrow cavity,high density of polythlene particles were injected into hole, titanium nail were put into, bone wax closed the window, then suturing step by step. After the molding, saline were used to gavaged in blank group and model group, Yougui recipe (see text) for Yougui recipe (see text) group, salmon calcitonin maximus injection for calcitonin group. After 10 weeks' mediation, rats were executed, and arterial blood and bilateral femoral organization were collected to biochemical, imaging morphology, tissue pathology and molecular biology detection.
RESULTSThe key gene expression of activiting osteoclast were inhibited in Yougui recipe (see text) group and calcitonin group. The level of OPG, Ca, ALP in Yougui recipe group were higher than calcitonin group (P<0.01); the content of RANKL were lower (P<0.01). There were no significance meaning in RANK, Trap5b, P between two groups.
CONCLUSIONBoth of Yougui recipe (see text) and calcitonin can slow and treat surrounding osteolysis of artificial joint prosthesis, and Yougui recipe (see text) has better effect in promoting bone formation. The effect of Yougui recipe (see text) in promoting bone formation, inhibiting osteoclasts to provide a new method to treating surrounding osteolysis of artificial joint prosthesis.
Animals ; Biomarkers ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; Gene Expression Regulation ; drug effects ; Interleukin-1 ; genetics ; Interleukin-6 ; genetics ; Male ; Osteolysis ; etiology ; metabolism ; pathology ; prevention & control ; Prostheses and Implants ; adverse effects ; Rats ; Rats, Sprague-Dawley ; Tomography, X-Ray Computed ; Vacuolar Proton-Translocating ATPases ; genetics
5.Construcion of a chimeric Japanese encephalits virus/dengue virus-2.
Yan WEI ; Peng LU ; Jian-shi YU ; Jian-dong LI ; Qin-zhi LIU ; Quan-fu ZHANG ; Chuan LI ; Fang MIAO ; Shuo ZHANG ; Xiao-tong HANG ; De-xin LI
Chinese Journal of Virology 2009;25(3):185-189
The prM/E gene of DV2 was cloned into the JEV (SA14-14-2 strain) replicon vector which had been constructed previously, and the resulting recombinant plasmid was named pPartialdeltaprM/E. The constructed chimeric clone was linearized and then was transcripted into RNA in vitro. The produced RNA was transfected into BHK-21 cells. Five to seven days later, CPE could be observed on the transfected BHK-21cells, and then the supernatant containing the chimeric virus was collected. The Supernatant was inoculated to BHK-1 cells and C6/36 cells, respectively. CPE could be observed about 4 days post the infection of C6/36cell with the chimeric virus. The results from RT-PCR, IFA, Western blot showed that the virus contained the chimeric RNA and the envelop protein of DV2. However, the chimeric virus could not be passaged in BHK-21 cell. The successful construction of the infectious clone JE/DEN-2 laid the basis for the further research of the DV vaccine.
Animals
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Blotting, Western
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Cell Line
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Cricetinae
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Dengue Virus
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genetics
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Encephalitis Viruses, Japanese
;
genetics
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Genetic Vectors
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genetics
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Reassortant Viruses
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genetics
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Recombination, Genetic
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genetics
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Reverse Transcriptase Polymerase Chain Reaction
6.Diagnosis and treatment of infective endocarditis in chronic hemodialysis patients.
Jian-Ling TAO ; Jie MA ; Guang-Li GE ; Li-Meng CHEN ; Hang LI ; Bao-Tong ZHOU ; Yang SUN ; Wen-Ling YEA ; Qi MIAO ; Xue-Mei LI ; Xue-Wang LI
Chinese Medical Sciences Journal 2010;25(3):135-139
OBJECTIVETo analyze the clinical features of hemodialysis patients complicated by infective endo carditis.
METHODSThe clinical features of six such patients admitted to Peking Union Medical College Hospital during the year 1990 to 2009 were analyzed. All of them were diagnosed based on Chinese Children Diagnostic Criteria for Infective Endocarditis.
RESULTSThe average age of the six patients was 52.3 +/- 19.3 years old. Four were males. Vascular accesses at the onset of infective endocarditis were as follows: permanent catheters in three, temporary catheters in two, and arteriovenous fistula in one. Three were found with mitral valve involvement, two with aortic valve involvement, and one with both. Five vegetations were found by transthoracic echocardiography, and one by transesophageal echocardiography. Four had positive blood culture results. The catheters were all removed. Four of the patients were improved by antibiotics treatment, in which two were still on hemodialysis in the following 14-24 months and the other two were lost to follow-up. One patient received surgery, but died of heart failure after further hemodialysis for three months. One was well on maintenance hemodialysis for three months after surgery.
CONCLUSIONSInfective endocarditis should be suspected when hemodialysis patients suffer from long-term fever, for which prompt blood culture and transthoracic echocardiography confirmation could be performed. Transesophageal echocardiography could be considered even when transthoracic echocardiography produces negative findings. With catheters removed, full course of appropriate sensitive antibiotics and surgery if indicated could improve the outcome of chronic hemodialysis patients complicated by infective endocarditis.
Adult ; Aged ; Aged, 80 and over ; Anti-Bacterial Agents ; therapeutic use ; Echocardiography, Transesophageal ; Endocarditis ; diagnosis ; drug therapy ; mortality ; Female ; Humans ; Kidney Failure, Chronic ; therapy ; Male ; Middle Aged ; Renal Dialysis ; adverse effects ; Risk Factors
7.Recombinant envelope glycoprotein domain III of dengue virus inhibit virus infection.
Peng LU ; Yan WEI ; Shou-Chun CAO ; Jian-Dong LI ; Qin-Zhi LIU ; Quan-Fu ZHANG ; Chuan LI ; Fang MIAO ; Shuo ZHANG ; Xiao-Tong HANG ; Mi-Fang LIANG ; De-Xin LI
Chinese Journal of Experimental and Clinical Virology 2008;22(3):177-179
OBJECTIVETo observe the ability of dengue virus recombinant envelope protein domain expressed in E. coli to inhibit virus infection and induce the neutralizing antibody.
METHODSE III protein of Dengue virus serotypes 1-4 were expressed in E. coli BL21(DE3) then purified. Recombinant proteins were tested to inhibit DV2 from infecting BHK-21 cell. Rabbits were immunized with recombinant proteins to produce anti-E III serum. Antibody titers were determined by neutralizing assay.
RESULTSThe recombinant E III proteins of Dengue virus serotypes 1-4 were expressed in E. coli. They effectively protected BHK cells in culture against DV2 infection. All four type anti-E III sera can neutralize DV2 but their efficacies are different.
CONCLUSIONproteins of dengue virus expressed in E. coli can directly inhibit DV2 infection. Neutralizing antibodies were induced by E III proteins. Both E III protein of dengue virus and the neutralizing antibodies they induced are more efficient in inhibiting homologous dengue serotypes infection than heterologous serotypes.
Animals ; Antibodies, Viral ; immunology ; Cell Line ; Cricetinae ; Dengue ; immunology ; prevention & control ; virology ; Dengue Virus ; chemistry ; genetics ; immunology ; physiology ; Down-Regulation ; Escherichia coli ; genetics ; metabolism ; Humans ; Immunization ; Mesocricetus ; Protein Structure, Tertiary ; Rabbits ; Recombinant Proteins ; chemistry ; genetics ; immunology ; Viral Envelope Proteins ; chemistry ; genetics ; immunology ; Virus Replication
8.Secreted expression of dengue virus type I envelope glycoprotein in 293T cells.
Fang MIAO ; Chuan LI ; Shuo ZHANG ; Xiao-fang WANG ; Jian-dong LI ; Quan-fu ZHANG ; Qin-zhi LIU ; Yan WEI ; Xiao-tong HANG ; Mi-fang LIANG ; De-xin LI
Chinese Journal of Experimental and Clinical Virology 2009;23(6):415-417
OBJECTIVETo expression prM/E gene of dengue virus type I in mammalia cells.
METHODSThe full-length prM/E gene of dengue virus type I strain GZ01/95 was amplified by RT-PCR, the signal peptide preceding the prM gene was added or the carboxyl-terminal 20% of DEN-1 E was replaced with the corresponding JE sequence in the meanwhile, and three of the constructions were cloned into the pcDNA5/FRT.Then they were transfected into 293T cells by lipofectamine respectively. The expression of recombinant proteins were identified by indirect immuno-fluorescence assay(IFA) as well as Western blot.
RESULTSIn the cytoplasm of 293T cells transfected with all the recombinant plasmids DNA, the expressed products for gene of dengue virus type I were confirmed by IFA. The secreted expression products for gene of dengue virus type I specific protein bands were confirmed by Western blot only existing in the cell supernatants transfected with the modified recombinant plasmids DNA.
CONCLUSIONThe prM/E protein of dengue virus type 1 were expressed in 293T cells transfected with all the three recombinant plasmids DNA. The prM/E protein was obtained secretion after transfecting the modified recombinant plasmids adding a signal peptide preceding the prM gene or replacing the carboxyl-terminal 20% of E with the corresponding JE sequence.
Cell Line ; Dengue ; virology ; Dengue Virus ; genetics ; metabolism ; Gene Expression ; Glycoproteins ; genetics ; metabolism ; Humans ; Protein Transport ; Recombinant Fusion Proteins ; genetics ; metabolism ; Viral Envelope Proteins ; genetics ; metabolism
9.Predicting value on short-term outcome of various established risk prediction models in extracorporeal membrane oxygenation treated cardiogenic shock patients due to ST-segment elevation myocardial infarction.
Shuo PANG ; Zi Ao RUI ; Yang DU ; Yuan Hang ZHOU ; Guang Rui MIAO ; Lu WANG ; Jian Zeng DONG ; Xiao Yan ZHAO
Chinese Journal of Cardiology 2022;50(9):881-887
Objective: To investigate the predicting value of different risk prediction models for short-term death in patients with ST-segment elevation myocardial infarction (STEMI) complicated by cardiogenic shock and treated with extracorporeal membrane oxygenation (ECMO). Methods: This study was a retrospective case-control study. Forty patients with STEMI complicated by cardiogenic shock who hospitalized in the First Affiliated Hospital of Zhengzhou University from April 2017 to August 2021 and treated with percutaneous coronary intervention (PCI) and ECMO, were enrolled in this study. Patients were divided into survival group and death group according to their clinical outcomes at 30 days after ECMO implantation, and clinical data of the two groups were collected and analyzed. Receiver operating characteristic (ROC) curve and decision curve analysis (DCA) were used to compare the predictive value of ACEF, AMI-ECMO, Encourage and SAVE risk scores for mortality at 30 days after ECMO implantation. According to the evaluation results of DCA, the optimal risk score was selected. Kaplan-Meier curve estimating the 30-day survival after ECMO implantation was plotted by grouping risk scores with reference to previous literatures. Results: A total of 40 patients with STEMI combined with cardiogenic shock were included, age was (57.4±16.7) years, 31 (77.5%) patients were male, there were 21 (52.5%) patients in the death group and 19 (47.5%) in the survival group. Compared with the survival group, patients in the death group had higher lactic acid values, higher proportion of anterior descending artery or left main artery lesions, and a higher proportion of acute renal failure and continuous renal replacement therapy during hospitalization (all P<0.05). Compared with survival group, ACEF, AMI-ECMO and Encourage scores were higher in death group, SAVE score was lower in death group (all P<0.05). The ROC curve analysis showed that the area under the curve (AUC) of ACEF, AMI-ECMO, Encourage and SAVE scores in predicting mortality were 0.707, 0.816, 0.757, and 0.677 respectively (P>0.05). ACEF score demonstrated the highest sensitivity (90.5%) and Encourage score exhibited the highest specificity (89.5%). DCA indicated that the AMI-ECMO and Encourage scores had the best performance in predicting the 30-day mortality after ECMO therapy. Kaplan-Meier survival curve analysis showed that the 30-day mortality after ECMO implantation increased with the increase of AMI-ECMO and Encourage scores (log-rank P≤0.001). Conclusions: The 4 scoring systems are all suitable for predicting 30-day mortality after VA-ECMO therapy in patients with ST-segment elevation myocardial infarction complicated by cardiogenic shock. Among them, AMI-ECMO and Encourage scores have better predicting performance.
Adult
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Aged
;
Case-Control Studies
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Extracorporeal Membrane Oxygenation/methods*
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Female
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Humans
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Male
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Middle Aged
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Percutaneous Coronary Intervention/methods*
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Retrospective Studies
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ST Elevation Myocardial Infarction/therapy*
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Shock, Cardiogenic/therapy*
10.Early effect of extracorporeal membrane oxygenation and factors related to early outcome in adult patients with fulminant myocarditis.
Yuan Hang ZHOU ; Xi ZHAO ; Ying Ying GUO ; Jia Ming YANG ; Dong Pu DAI ; Zi Ao RUI ; Yang DU ; Shuo PANG ; Guang Rui MIAO ; Xiao Fang WANG ; Xiao Yan ZHAO ; Jian Zeng DONG
Chinese Journal of Cardiology 2022;50(3):270-276
Objective: To evaluate the efficacy within the first 24 h post extracorporeal membrane pulmonary oxygenation (ECMO) and the impact of early efficacy on the prognosis of adult patients with fulminant myocarditis (FM). Methods: This retrospective case analysis study included hospitalized patients (age≥18 years) who were diagnosed with fulminant myocarditis from November 2016 to May 2021 in the First Affiliated Hospital of Zhengzhou University. Patients were divided into survival or non-survival groups according to treatment outcomes. The age, sex, treatments, drug use, ECMO use, clinical and laboratory data (before and 24 h after the use of ECMO) were analyzed. The change rate of clinical and laboratory data after 24 h use of ECMO was calculated to find differences between two groups. Multivariate logistic regression was used to analyze the related factors with in-hospital death and complication between the two groups. Results: A total of 38 FM patients treated with ECMO were included. There were 23 cases (60.5%) in the survival group, aged (39.6±13.7) years, and 17 (73.9%) cases were female. The total ECMO time was (134.4±71.3)h. There were 15 cases (39.5%) in non-survival group, aged (40.0±15.8) years, and there were 12(80.0%) female, the ECMO time was (120.1±72.4) h in this group. The proportion of tracheal intubation and continuous renal replacement therapy in the survivor group and dosage of norepinephrine within 24 h after ECMO implantation were significantly less than in non-survival group (all P<0.05). There was no significant difference in all efficacy related biochemical indexes between two groups before ECMO use. The levels of lactic acid, procalcitonin, creatinine, alanine aminotransferase, aspartate aminotransferase, creatine kinase-MB, cardiac troponin I and N-terminal B-type natriuretic peptide prosoma were significantly less in survival group than in non-survival group at 24 h after the use of ECMO (all P<0.05). Results of multivariate logistic regression analysis showed that the higher 24 h change rate of creatinine (OR=0.587, 95%CI 0.349-0.986, P=0.044) and creatine kinase-MB (OR=0.177, 95%CI 0.037-0.841, P=0.029) were positively correlated with reduced risk of in-hospital mortality. The central hemorrhage and acute kidney injury in survival group were less than in non-survivor group (P<0.05). Conclusions: After 24 h early use of ECMO in FM patients, the improvement of various efficacy related biochemical test indexes in the survival group was better than that in the non-survival group. Faster reduction of creatine kinase-MB and creatinine values within 24 h ECMO use is positively correlated with reduced risk of in-hospital mortality in adult patients with FM.
Adolescent
;
Adult
;
Extracorporeal Membrane Oxygenation/methods*
;
Female
;
Hospital Mortality
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Humans
;
Middle Aged
;
Myocarditis/therapy*
;
Retrospective Studies
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Treatment Outcome
;
Young Adult