Natamycin is a polyene macrolide antifungal antibiotic ,which is wide ly used in the food industry in order to prevent mould contamination .Biosynthe s is gene cluster of natamycin is discovered by the overall of progress in molecul ar biology of natamycin, including 16 open reading frames which includes the gen e for 26-member ring formation of natamycin (pimS0-pimS4 ) and the modifying gene s, and the function of the protein including polyketide synthases(PKSs)、PimD、P imJ、PimK were studied

OBJECTIVETo analyze the relationship between inorganic ingredients (Ca, P and F) concentration of plaque residues in the primary teeth and caries status, sugar exposure frequency.

METHODSSubjects were 93 of 3-5 years old children living in Beijing area. According to their caries status, they were classified into two groups: Severe early childhood caries(S-ECC) group and caries free(CF) group. Plaque samples were collected from subjects respectively. Ca, P and F concentration of plaque residues was measured by atomic absorption spectroscopy, spectrophotometer and fluoride electrode respectively. The information of daily sugar exposure frequency of children was obtained from a standardized questionnaire filled by their parents. The relationship between inorganic ingredients (Ca, P and F) concentration of plaque residues and daily sugar exposure frequency was analyzed and the relationship between inorganic ingredients (Ca, P and F) concentration of plaque residues and caries status in the S-ECC group was also analyzed.

RESULTSThe mean values for Ca, P and F concentration of plaque residues were lower in the S-ECC group, but the difference was not statistically significant (P > 0.05). The daily sugar exposure frequency in the S-ECC group was higher than that in the CF group. There were no relationships between Ca, P and F concentration of plaque residues and caries status (dmft, dmfs), sugar exposure frequency in the primary dentition.

CONCLUSIONPlaque residues from CF subjects did not contain a greater pool of calcium, inorganic phosphorus, and fluoride ions than that from S-ECC subjects.

Calcium ; Carbohydrates ; Child ; Child, Preschool ; Dental Caries ; Dental Caries Susceptibility ; Dental Plaque ; Fluorides ; Humans ; Phosphates ; Tooth, Deciduous

Objective To assess the chest X-ray radiographic findings in children with influenza A (H1N1) virus infection. Methods The chest X-ray radiographies in 67 children with influenza A(H1N1) virus infection were reviewed in this study. The chest radiographies were obtained 3-8 days after the onset of symptoms and for the follow-up. Results The abnormalities were bilateral in 53 patients and unilateral in 7 patients. The predominant radiographic findings were bilateral patchy consolidation (n=42) with rapid confluence in 10 patients, lobular consolidation (n=7) with interstitial hyperplasia in 1 patient 3 month later, diffuse consolidation (n=11) with interstitial hyperplasia in all patients after 3 month. ConclusionThe predominant chest X-ray radiographic findings are bilateral patchy consolidation and diffuse consolidation with interstitial hyperplasia afterward.

Aim To evaluate the bioequivalence of demestic brodimoprim capsules and imported hyprim tablets and provide experimental basis for clinical application. MethodsA single dosage of Brodimoprim or hyprim was given to 18 healthy volunteers in a randomized 2-way cross-over test and the brodimoprim concentrations in plasma were determined by HPLC with β-naphtol as internal standard. The pharmacokinetic parameters and the relative bioavailability of the two preparations were calculated and their bioequivalence was evaluated. ResultsThe major pharmacokinetic parameters of test and reference preparations were as follows respectively:t1/2(α) (2.1 + 1.0) and (1.9+± 0.9) h, t1/3(β)(43.2±4.8) and (42.4±4.3)h, Tpeak(3.4±1.6) and (3.1±1.5) h,Cmax(5.9+ 0.9) and (5.9±1.0)μg · ml-1, AUC0～132(360.2± 55.3) and (358.7±52.6) μg · h · ml-1, AUC0～∞ (423.8±56.0) and (422.5±51.1) μg · h · ml-1. The relative bioavailability(F) of brodimprim capsules was (99.7± 4.8)%。 Conclusion . The multi-factorial analysis of variance showed that there was no significant difference in AUC0- 132between the test and reference preparations (P＞ 0.05) . The bioequivalent assumption was proved by further two one-side t-test and (1～2 α) confidence interval analysis in individuals, periods and forms of these two preparations.

Acetylcholine ; pharmacology ; Adenocarcinoma ; metabolism ; Cell Line, Tumor ; Gastric Mucosa ; cytology ; drug effects ; metabolism ; Humans ; Receptors, Cholinergic ; metabolism ; Stomach Neoplasms ; metabolism

Objective To study inhibitory activity of anthraquinone in Polygonum Cillinerve(Nakai) Ohwi (anthraquinone in PCO) to nitrosation. Methods Anthraquinone was extracted from Polygonum Cillinerve(Nakai) Ohwi with sulfuric acid and chloroform as solvents. The capability of scavenging sodium nitrite and disconnecting nitrosamine synthesis with anthraquinone in PCO were determined by spectrophotometry under simulated human gastric juice. Results Both the sodium nitrite scavenging rate and the nitrosamine synthesis disconnecting rate showed the positive correlation with the concentration of anthraquinone in PCO. The strongest capability of scavenging sodium nitrite was 53.5%, and the strongest capability of disconnecting nitrosamine synthesis was 71.3%. Conclusion Anthraquinone in PCO had strong capability of scavenging sodium nitrite and disconnecting nitrosamine synthesis.

Acetylcholine ; pharmacology ; Cell Line, Tumor ; Cell Membrane ; Epithelial Cells ; cytology ; Gastric Mucosa ; cytology ; drug effects ; metabolism ; Humans ; Receptors, Nicotinic ; drug effects ; metabolism ; Stomach Neoplasms ; metabolism

Congresses as Topic ; Humans ; Otorhinolaryngologic Surgical Procedures

OBJECTIVE:To establish a method for the simultaneous determination of narirutin,naringin,hesperiden and neo-hesperiden in Weisu granule. METHODS:UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile-0.2% Phosphoric acid aqueous(gradient elution)at a flow rate of 0.40 ml/min,the detection wavelength was 284 nm, the column temperature was 30 ℃,and the injection volume was 1 μl. RESULTS:The linear range was 9.38-93.75 μg/ml for narirutin(r=0.999 7), 32.25-322.50 μg/ml for naringin(r=0.999 7), 11.25-112.50 μg/ml for hesperiden(r=0.999 9) and 11.88-118.75 μg/ml for neohesperidin(r=0.999 8);limits of quantitation were 20 ng,18 ng,18 ng and 18 ng,the limits of detec-tion were 6 ng,5 ng ,5 ng and 5 ng,respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;re-coveries were 96.24%-103.12%(RSD=2.45%,n=6),98.43%-102.10%(RSD=1.42%,n=6),96.10%-101.41%(RSD=2.07%,n=6)and 95.57%-99.06%(RSD=1.44%,n=6),respectively. CONCLUSIONS:The method is rapid and efficient,and suitable for the simultaneous determination of narirutin,naringin,hesperiden and neohesperiden in Weisu granule.

Objective To establish the optimum preparation process for Syrigopicroside Dropping Pills.Methods The preparation conditions,such as the proportion between the extracts and matrix,dripping temperature,the ratio of matrix,were studied with orthogonal test.The obtained results were tested by three indexes,such as the variation coefficient of weight of pill,time limit of dissolution and appearance of the dropping pills with analysis of variance.The preparation process was optimized.Results The optimal technical conditions were as follows:the ratio of PEG4000 and PEG6000 as matrix was 4∶1,the ratio of syrigopicroside to matrix was 1∶4,and the dripping temperature was 80 ℃.Conclusion The preparation technology can meet the requirement for dripping pill specified in China Pharmacopeia(2005 Edition).