Objective To establish a method for rapidly detecting the G-protein ?3 subunit (GNB3) 825 site single nucleotide polymorphism (SNP) and to analyse the relationship between GNB3 825 site gene SNP and obesity. Methods The real-time fluorescent PCR was employed to analyse the GNB3 825 site gene SNP of 420 samples from 21 provinces and the the frequencies of genotypes were compared with those detected by gene sequencing. GNB3 825 site genotype, body weight, body mass index (BMI) and fat content were examined from 207 subjects and the correlation between GNB3 825 site gene SNP and obesity was analysed. Results The result by real-time fluorescent PCR showed that the frequencies of 825T and 825C haploid were 46.90% and 53.10%, respectively, and the frequencies of 825TT, 825TC and 825CC genotype were 22.38%, 51.42% and 28.10%, respectively, with no other genotype detected, which was consistent with the result by gene sequencing. BMI and fat content were significantly higher in subjects with GNB3 825TT than in subjects with other genotypes. Body weight was much higher in subjects with GNB3 825TT genotype than in subjects with 825CC genotype, but not significantly different with 825CT genotype. Conclusion A new rapid method for the detection of GNB3 825 site SNP has been successfully established. There existed significant correlation between GNB3 825TT genotype and obesity.

OBJECTIVETo explore the differences of electroacupuncture (EA) on onset time and symptom improvement for treatment of different types of functional constipation.

METHODSThirty-eight cases of constipation were selected, including 9 cases of constipation-predominant irritable bowel syndrome (IBS-C), 10 cases of slow transit constipation (STC), 10 cases of pelvic floor dyssynergia (PFD) and 9 cases of inadequate defecatory propulsion (IDP). The electroacupuncture was applied at Tianshu (ST 25), Fujie (SP 14), Shenshu (BL 23), Dachangshu (BL 25) and so on in abdominal and lumbosacral area, 5 times per week, 10 times as a treatment course. The onset time, score of clinical symptoms of constipation and improvement of every symptom in each group were compared.

RESULTS(1) The onset time was (1.78 +/- 0.83) days in IBS-C type, (3.11 +/- 1.90) days in IDP type, (4.10 +/- 1.85) days in STC type and (4.30 +/- 2.00) days in PFD type, indicating statistical differences between IBS-C type and STC type, IBS-C type and PFD type (both P < 0.05). (2) Compared before the treatment, the total scores of symptoms on the onset day in each group were all improved (P < 0.05, P < 0.01), and score of IBS-C type was superior to the rest 3 types (P < 0.05, P < 0.01). (3) EA improved desire to defecate or frequency of defecation in each type (P < 0.05, P < 0.01), in which both were improved in STC type and PFD type, and the improvement of defecation frequency was more significant in STC type (P < 0.01). EA relieved unsmooth defecation or pendant-expansion feeling in each type (all P < 0.05), in which both were improved in IBS-C type (both P < 0.05). EA relieved abdominal distension and pain in IBS-C type, STC type and PFD type (all P < 0.05), while its effects were not obvious on defecation difficulty, defecation time and defecation texture (all P > 0.05).

CONCLUSIONThe electroacupuncture for treatment of 4 types of constipation is characterized by rapid onset; the improved symptoms are not identical in the electroacupuncture treatment plan for each type of defecation; the main improvement of symptoms are lied on desire to defecate and frequency of defecation, unsmooth defecation or pendant-expansion feeling and abdominal distension and pain. Meanwhile the improvements of defecation texture, defecation difficulty and defecation time were not signi-ficant.

Adult ; Aged ; Constipation ; etiology ; physiopathology ; therapy ; Defecation ; Electroacupuncture ; Female ; Humans ; Irritable Bowel Syndrome ; complications ; Male ; Middle Aged ; Pelvic Floor Disorders ; complications ; Treatment Outcome ; Young Adult

Objective To investigate the inhibitory effects of norcantharidin (NCTD),paclitaxel (PTX) and their combination on androgenic independent prostate cancer cells.Methods The experiment was divided into 3 groups:NCTD group,PTX group and combination group (NCTD + PTX).NCTD group and PTX group:In the exponential growth of the prostate cancer cell line DU145,different concentrations (12.5,25.0,50.0,100.0,200.0,400.0,800.0 μmol · L-1)of NCTD and PTX were added.Combination group:NCTD and PTX according to the ratio of 1:1 concentration,repeat first group operation.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution 20 μL was added after 48 h.The culture supematant liquid was extracted after train 4 h,DMSO 200 μL was added in order to dissolve.The recovery rate of DU145 and the inhibition rate of cell growth were measured by centrifugation.half inhibitory concentration was determined by MTT.Whether the two drugs have synergistic effect was investigated by simulating the joint index.Results The inhibition rate of the above 7 concentration of NCTD group were 0.12％,0.14％,0.25％,0.30％,0.58％,0.70％,0.74％.That of the above 7 concentration of NCTD group were 0.12％,0.14％,0.25％,0.30％,0.58％,0.70％,0.74％.The inhibition rate of combined group was higher than that of NCTD group and PTX group.The difference was statistically significant (all P ＜ 0.05).With the increase of the dosage of two drugs,the simulated joint index (CI) value was higher and lower than 1.The lowest of CI was 0.07 ± 0.01,with a maximum of 0.59 ± 0.20.Compared with the lowest concentration,the difference was statistically significant (P ＜ 0.05).The results showed that when NCTD and PTX were combined,there was a synergistic effect between the two groups.Conclusion Both NCTD and PTX have inhibitory effects on androgenic independent prostate cancer cells.The higher of the drug contents,the stronger of the inhibitory effect.The combined use of NCTD and PTX has stronger inhibitory effect on cancer cells.

Objective To investigate the protective effects of polydatin on performance of sepsis injury of renal glomerular vascular endothelial and the activity of adenosine monophosphate (AMP)-activated protein kinase(AMPK) and mammalian target protein (rmTOR)in the process.Methods The sepsis model was established by injection of lipopolysaccharide through the tail vein of rats.The rats were divided into four groups:sham operated group (implementation of anesthesia,intubation),model group (anesthesia,intubation and sepsis),control group (anesthesia,intubation and application of saline resuscitation),experimental group (anesthesia,intubation and sepsis,saline combined with tiger polydatin 10 kU · kg-1).The activity of AMPK and rmTOR in each groups were detected by ELISA.Results After administration for 6 h,the heart rate (beat per minute) in experimental group,control group,model group were 88.21 ± 17,102.36 ± 12.83,118.04 ± 16.51.The urea nitrogen(BUN) in the three groups were (49.77 ±9.67),(77.92 ± 12.01),(122.19 ± 12.87) mg · dL-1.Compared with the control and model groups,all of them in experimental group were statistically significant (all P ＜ 0.05).The protein activeties of AMPK and mTOR in experimental group,model group were respectively 3.06 ± 1.34,0.77 ± 0.21;2.48 ± 1.12,3.95 ± 1.02.Compared with model groups,all of them in experimental group were statistically significant (all P ＜ 0.05).Conclusion Polydatin had protective effect on septic renal damage glomerular vascular endothelial,which might be correlated with its inhibition on mTOR activity through AMPK pathway.

Objective To evalute the efficacy of high-resolution CT(HRCT) in differential diagnosis and treatment of chronic suppurative otitis media and cholesteatoma otitis media by soft-tissue shadows. Methods HRCT scanning was performed in 120 cases, 153 ears, with chronic otitis suppurative media and cholesteatoma otitis media, of which original data were processed with multi-planar reconstructtion (MPR) and maximum intensity projection ( MIP) , the characteristics of the soft-tissue shadows' growth, window width or window leveling and bony destruction were respectively observed, as well as compared with the surgery findings. Results In 120 patients (133 ears), 109 ears were diagnosed as cholesteatoma otitis media, and 44 ears were diagnosed as chronic suppurative otitis media, among which 33 ears had granulation tissue and 11 ears had secretion. One hundred and seven ears were postoperatively diagnosed as cholesteatoma otitis media, among which 25 ears had granulation tissue. Among 46 ears of chronic suppurative otitis media, 35 ears had granulation tissue, and only 11 ears had secretion. A 98. 6% diagnostic accuracy can be reached with HRCT in diagnosing cholesteatoma otitis media and chronic suppurative otitis media. The Youden's index was 0. 98, 0. 98 and 1. 00 respectively with HRCT in diagnosing cholesteatoma, granulation tissue and secretion. Conclusions Combination of the three different imaging methods, axial images, coronal MPR images and MIP images, can improve the efficacy of the HRCT diagnosis and definite chronic otitis media, which can be routinely used for surgery plan.

Estrogen receptor alpha (ERalpha) has been a primary target of treatment as well as a prognostic indicator for breast cancer. The level of human X-box binding protein 1 (XBP-1) mRNA was related with that of ERalpha in breast tumors and was over-expressed in some breast tumors. These previous studies suggested that XBP-1 may interact with ERalpha. XBP-1 has two isoforms, XBP-1S and XBP-1U, as the result of unique splicing. GST pull-down assay showed that both XBP-1S and XBP-1U bound to ERalpha in vitro. The binding of XBP-1S to ERalpha was stronger than that of XBP-1U to ERalpha. Co-immunoprecipitation revealed that the binding was in a ligand-independent manner. XBP-1S and XBP-1U interacted with the region of ERalpha that contains a DNA-binding domain. The ERalpha-interacting regions on XBP-1S and XBP-1U have been mapped to two regions, the N-terminal basic region leucine zipper domain (bzip) and the C-terminal activation domain. These findings suggest that XBP-1S and XBP-1U may participate in ERalpha signaling pathway through the mediation of ERalpha.
Breast Neoplasms ; genetics ; metabolism ; Cell Line, Tumor ; DNA-Binding Proteins ; genetics ; metabolism ; Estrogen Receptor alpha ; genetics ; metabolism ; Female ; Humans ; Protein Interaction Domains and Motifs ; physiology ; RNA, Messenger ; biosynthesis ; genetics ; Regulatory Factor X Transcription Factors ; Signal Transduction ; Transcription Factors ; genetics ; metabolism ; X-Box Binding Protein 1

OBJECTIVETo study the effects of Erlong Zuoci Pill (, ELZCP) and its disassembled: prescriptions on gentamicin (GM)-induced ototoxicity model in vitro.

METHODSAfter the spiral organ of cochleae: of newborn mice (postnatal days: 2-3) cultured for 24 h, GM alone or combined with water extracting-alcohol precipitating solution of ELZCP or with its disassembled prescriptions was added. Hair cells were observed under a fluorescence microscope after TRITC-phalloidin staining, and the cochlear hair cell loss rate was calculated by counting the whole cochlear hair cells and analyzed by whole cochlear hair cells analyzing software.

RESULTSGM induced cochlear outer hair cells (OHCs) and inner hair cells (IHCs) injuries in a dose-dependent manner, and they were significantly different as compared with those in the normal control group (P<0.05, P<0.01). ELZCP at the concentration of 0.003-3 mg/mL could decrease the hair cells loss induced by the 0.3 mmol/L GM (P<0.05, P<0.01), the effects was in a dose-dependent manner, and the concentration of 0.3 mg/mL showed the optimal protective effect. For the ELZCP disassembled prescriptions, Liuwei-Dihuang could decrease OHC loss rate than that in the 0.3 mmol/L GM model group (P<0.05), but the OHC loss rate was still higher than that in the ELZCP group (P<0.01), which indicated that the protective effect of hair cells by Liuwei-Dihuang was not better than that of ELZCP. Poria decreased OHC loss rate from 72.1 % +/-3.7 % to 58.8 %+/- 8.2 % (P<0.05).

CONCLUSIONSELZCP could play a role in antagonizing the injury of cochlear hair cells induced by GM ototoxicity,: and its disassembled prescriptions, Liuwei-Dihuang was the main component to protect the cochlear hair cells from GM-induced ototoxicity, and Magnetitum combined with Radix Bupleurui could strengthen the action of the whole prescription; Poria could reduce GM-induced OHC loss.

Animals ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; pharmacology ; Gentamicins ; toxicity ; Hair Cells, Auditory, Inner ; drug effects ; pathology ; Hair Cells, Auditory, Outer ; drug effects ; pathology ; Mice ; Organ of Corti ; drug effects ; pathology ; Prescriptions ; Tablets

Objective To evaluate clinical significance of monitoring noninvasive intracranial pressure (NICP) and cerebral perfusion pressure (CPP) in treatment of patients with hypertensive intracerebral hemorrhage. Methods This clinical randomized controlled trial enrolled 120 patients with hypertensive intracerebral hemorrhage who had sought medical treatment in our department from June 2008 through May 2010. They were randomized equally into a monitoring group where NICP and CPP were continuously monitored before and after operation and a non-monitoring group where no monitoring of NICP and CPP was performed. Results In the monitoring group,increased NICP and decreased CPP were shown in 50 patients and only 10 patients were shown with normal NICP (＜26.6mmHg) and CPP (＞ 124.3 mm Hg). The abnormal NICP and CPP continuously monitored were treated with specific interventions like further operation or medication. In the non-monitoring group,patients received only conventional treatments.According to the Glasgow Outcome Scale (GOS), 31 patients (51.7％) had good recovery,20 (33.3％) were moderately disabled,5 (8.3％) severely disabled and 4 (6.7％) dead in the monitoring group while 23 (38.3％) patients had good recovery,18 (30.0％)were moderately disabled,10 (16.7％) severely disabled and 9 (15.0％) dead in the non-monitoring group.The outcomes of the monitoring group were significantly better than those of the non-monitoring group (P＜0.05). Conclusion Continuous monitoring of NICP and CPP before and after operation should be performed in the treatment of patients with hypertensive intracerebral hemorrhage because it is helpful for clinical medication and reducing complications and mortality as well.

Objective To research the intervention effect with Lamaze non-drag methods to allevate pains for lying-in woman during stay and delivery periods for natural childbearing. Methods Taking 300 pregnant women and dividing them into two groups: the intervention group of 150 pregant women and the contral group of 150 pregnant women. In the intervention group, 150 pregnant women have trained Lamaze non-drag methods to allevate pain from 7th fetation and practised them in stay and spontaneous delivery periods. In control group, 150 pregnant women were not given the trainings, but spontaneously deliveryed with the routine one to one nursing care. Results The time of first stage, the time of second stage, and the total time of the two stages of labor in the intervention group were statistically shorter than those in control group. Labor pain was much alleviated in the intervention group, but not in control group. PPH, and neonatal asphysxia in the intervention group were statistically less than those in the control group. Conclusions Lamaze non-drag method is not only safe and effective to allevate pain for lying-in woman, but also is possible to shorten total labor time, decrease Postpartum Hemorrhage (PPH) and neonatal asphysxia.

OBJECTIVETo seek the problems of position, measuring and locating methods of Baliao points (posterior sacral foramina) in modern researches.

METHODSUsing Baliao (eight sacral foramina), Shangliao(BL 31), Ciliao(BL 32), Zhongliao(BL 33), Xialiao(BL 34), Dihoukong (posterior sacral foramina), Dikong (sacral foramina) and Digu(sacrum) as the key words, literature in the database of the CNKI from 1957 to 2012 were re trieved and analyzed.

RESULTSProblems were found in the past researches including limited numbers of relative literature, disunity of the measurement targets, complicated terms of indices, disunity of the starting and ending point of measurement, unclear weight of indices, deviation of results, lacking of combination with clinical practice and variety of locating methods.

CONCLUSIONPosition of Baliao points (eight sacral foramina) are clear. However, the locating methods are blurred and vary a lot. Study on living body has more significance for measurement and researches. Factors of gender, body weight, height and childbearing should also be taken into consideration. Therefore, it is necessary to find a more accurate and easier way of locating.

Acupuncture Points ; Acupuncture Therapy ; Humans ; Sacrum ; anatomy & histology ; diagnostic imaging ; Tomography, X-Ray Computed