1.Effect and mechanism of intravenous bone marrow stromal cells transplantation against chronic ethanol-induced cognitive dysfunction in rats
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(2):101-103
Objective To investigate the effect and mechanism of intravenous bone marrow stromal cells (BMSCs)transplantation against chronic ethanol-induced cognitive dysfunction in rats.Methods BMSCs from rats were isolated,cultured and identified in vitro.Male Sprague-Dawley rats(n=28)were randomly divided into control group(n=7),chronic ethanol group(n=7),BMSCs group(n=7)and PBS group(n=7).BMSCs(1×10~7 cells/animal)or PBS were intravenously injected to the rats of BMSCs or PBS group at the 7th week after ethanol gavage,respectively.All rats were sacrificed at the end of the 8th week.The learning and memory behavior was assessed using Moms water maze task.Immunofluorescence was used to examine cell proliferation maker(BrdU)in hippocampal dentate gyrus.Immunohistochemistry was used to evaluate NGF and BDNF expression in hippocampal region.Results In Morris water maze,escape latency at each time point in BMSCs group((33.83±3.26)s,(13.14±2.64)s,(6.53±0.98)s,(4.45±0.85)s)were significantly shorter than those in chronic ethanol group((53.78±4.29)s,(36.53±2.39)s,(18.28±2.13)s,(8.67±0.46)s,all P<0.01))and time percentage of platform quadrant in BMSCs group((73.99±1.49)%) was dramatically longer than that in chronic ethanol group((51.25±3.69)%),but no significant difference with contro1.The number of BrdU-positive cells in the dentate gyrus was significantly higher in BMSCs group than that in chronic ethanol group(P<0.01).Comparing to chronic ethanol gavage,BMSCs transplantation significantly increased the expression of NGF alld BDNF (P<0.01)in hippocampus.Conclusion Intravenous BMSCs transplantation is able to result in enormous attennation of the learning and memory abnormalities in chonic alcoholism rats,and which might be involved to promote hippocampal endogenous cellular proliferation by secretary neurotrophic factors.
2.Comparison of ultrasound-guided transperineal and transrectal prostate biopsies with 12+X method
Xiangshu BO ; Xiaoning SUN ; Jian TAN
Clinical Medicine of China 2016;32(9):803-806
Objective To compare the positive rates and complications of ultrasound?guided transperi?neal and transrectal prostate biopsies with 12+X method. Methods From January 2011 to January 2015,252 cases patients underwent ultrasound?guided prostate biopsies with 12+X method for suspected prostate carcinoma were selected as research subjects,including 114 cases were perineal puncture( transperineal group) and 138 ca?ses were transrectal puncture( transrectal group) . The positive rates and complications of biopsy were compared between the two groups. Results The positive rates in the transperineal and transrectal groups were 43. 5%( 50/114) and 41. 3%( 57/138) ,and there was no significant difference( P>0. 05) . No statistically significant differ?ences were observed between the two groups according to different PSA levels(≤4. 0,>4. 0-≤10. 0,>10. 0-≤20. 0,>20. 0 μg/L) ( P>0. 05) . There were no significant differences between two groups in complications such as:hematuria,lower urinary tract symptoms and acute urinary retention( P>0. 05) . The incidence rates of post?biopsy infection and rectal bleeding were remarkably lower(2. 6%(3/114) vs. 13. 0%(18/138),1. 8%( 2/114) vs. 11. 6%( 6/138) ,P=0. 005,0. 002) in transperineal group than in transrectal group. However,the perineal swelling rate was remarkably higher in the transperineal group than that in the transrectal group( 8. 8%( 10/114) vs. 0. 7%( 11/138) ,P=0. 003) . Conclusion Ultrasound?guided transperineal and transrectal pros?tate biopsies with 12+X method are both effective for the diagnosis of prostate carcinoma. The positive rates of the two approaches are similar,but the complications are different. The choice between the two approaches depended on the clinical features of the patients.
3.Influence of IL-35 on occurrence and development of heart failure
Chinese Journal of cardiovascular Rehabilitation Medicine 2015;24(6):673-676
Heart failure is a syndrom of heart disease with high morbidity and mortality .There are more and more evidence indicating immune activation developing important role in the occurrence and development of heart failure . Interleukin (IL)‐35 ,known as a negative regulatory factor of one of the members of IL‐12 family ,participates pathogenetic process of heart failure .The present article made a review for influence of IL‐35 on heart failure .
4.Experience in diagnosis and treatment of neonate with congenital glossal root cyst.
Ke-bo GONG ; Xiao-wei SUN ; Jian-ji ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(12):1044-1045
Cysts
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congenital
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diagnosis
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surgery
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Female
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Humans
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Infant, Newborn
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Male
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Tongue Diseases
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congenital
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diagnosis
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surgery
6.Median effective target effect-site concentration of sufentanil inhibiting responses to insertion of laryngeal mask airway when combined with propofol in elderly patients
Ling LIU ; Bo LI ; Jian SUN ; Guoyi LYU
Chinese Journal of Anesthesiology 2014;34(7):824-825
Objective To determine the median effective target effect-site concentration (EC50) of sufentanil inhibiting responses to insertion of laryngeal mask airway (LMA) when combined with propofol in the elderly patients.Methods ASA physical status Ⅰ or Ⅱ patients,aged 66-75 yr,with body mass index of 19-30 kg/m2,scheduled for elective transurethral resection of prostate under general anesthesia,were included in this study.Anesthesia was induced with propofol given by target-controlled infusion.The initial target effect-site concentration (Ce) of propofol was 1 μg/ml,and the Ce was increased by 0.5 μg/ml every 1 min until it was increased to 2 μg/ml and maintained at this level for 5 min.Sufentanil was simultaneously administered by target-controlled infusion.The target Ce of sufentanil was 0.3 ng/ml in the first patient.The concentration of sufentanil was determined using the modified Dixon's up-and-down method.After the patients lost consciousness,LMA was inserted after the target effect-site and plasma concentrations were balanced.The insertion response was defined as positive when MAP and HR increased by 20% of the baseline value within 5 min after insertion of LMA.The Ce of sufentanil was increased/decreased in the next patient if the insertion response was positive or negative.The ratio between the two successive concentrations was 1.2.The EC50 and 95 % confidence interval of sufentanil blunting responses to insertion of LMA was calculated using Probit analysis.Results When combined with propofol,the EC50 (95% confidence interval) of sufentanil blunting responses to insertion of LMA was 0.160 (0.130-0.188) ng/ml.Conclusion The EC50 of sufentanil inhibiting responses to insertion of LMA is 0.160 ng/ml when combined with propofol in the elderly patients.
7.Norovirus in Hospital:Its Epidemiological Investigation
Haiyan LI ; Bo JIANG ; Zhijun SUN ; Hongyan CHEN ; Jian LI
Chinese Journal of Nosocomiology 2006;0(02):-
OBJECTIVE To investigate the transmission process of large scale abrupt diarrhea with unknown reason occurred in hospital,analyze the cause of infection outbreak and the characteristics of Norovirus in hospital and to provide evidence to prevent and control the infection in hospital.METHODS Standardized questionnaire was used on individual,looking over the case of illness and the investigation of outbreak scene,the relationship of various contact type and odds of infection.RESULTS Nine cases were occurred,fifty-two cases were contacted,The morbidity was 17.31%.The feature of Norovirus infection was clear.CONCLUSIONS The first source of infection was an input case.The main transmission way is no protecting close contact.The high risk group is inpatients and their relatives.
8.Initial Discussion About How To Improve Orthopedic Clinical Teaching Quality For Externs
Hong LI ; Shao-Bo LI ; Chao-Jun SUN ; Jian-Bo ZHAO ;
Chinese Journal of Medical Education Research 2006;0(10):-
During the orthopedic clinical teaching for interns,such methods as raising high-toned professional ethics,strengthening basic skills of orthopedics developing all-round scientific clinical thought to guide interns to correctly treat employment and taking the entrance exams for postgraduate schools,so as to improve the orthopedic teaching quality and attain the target of orthopedic clinical teaching.
9.Chicory extract's influence on gut bacteria of abdominal obesity rat.
Bo-Yu SUN ; Bing ZHANG ; Zhi-Jian LIN ; Li-Yu LI ; Hong-Po WANG ; Jun ZHOU
China Journal of Chinese Materia Medica 2014;39(11):2081-2085
OBJECTIVETo investigate the efficacy mechanisme of chicory extract interventing abdominal obesity rat from the aspect of gut bacteria.
METHODMale SD rats were randomly divided into five groups, namely the normal group, model group, large and small dose group of chicory and the fenofibrate group. Normal group was given deionized water, the other group was given fructose water and give the medical treatment of chicory and fenofibrate. Assay triglycerides, total cholesterol, LDL and HDL by biochemical methods and measure body weight and abdominal circumference and microscopicly observe the count changes of gut bacteria through real-time PCR method.
RESULTCompared with normal group, the triglyceride level and abdominal circumference were significantly higher (P < 0.05), weight and high-density lipoprotein increased but no significant changes and E. coli, lactobacillus increased significantly. Compared with model group, chicory extract large and small dose group and the fenofibrate group can significantly reduce triglyceride levels (P < 0.05), reduce the number of E. coli and Lactobacillus and increase the number of bifidobacteria. The fenofibrate group can significantly reduce total cholesterol and high-density lipoprotein levels.
CONCLUSIONThe chicory's treatment effect on abdominal obesity is significant. The efficacy mechanisme intervention abdominal obesity may be related to the reduction of the number of lactic acid bacteria and E. coli and the increase of bifidobacteria.
Animals ; Bacteria ; classification ; genetics ; isolation & purification ; Biodiversity ; Chicory ; chemistry ; metabolism ; Cholesterol ; metabolism ; Gastrointestinal Tract ; microbiology ; Humans ; Male ; Microbiota ; Obesity, Abdominal ; metabolism ; microbiology ; Plant Extracts ; metabolism ; Rats ; Rats, Sprague-Dawley ; Triglycerides ; metabolism
10.Chondroblastoma with associated aneurysmal bone cyst of the talus: a case report and review of relative literatures.
Bo SUN ; Xue-yin LI ; Xing-yu ZHAO ; Feng WEI ; Jian-guo LIU
China Journal of Orthopaedics and Traumatology 2015;28(7):657-659
Adult
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Bone Cysts, Aneurysmal
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diagnosis
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surgery
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Bone Neoplasms
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diagnosis
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surgery
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Chondroblastoma
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diagnosis
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surgery
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Female
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Humans
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Male
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Talus
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surgery
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Young Adult