1.Construction of lentiviral vector of microRNA targeting IGF1R gene and its inhibition effect on liver cancer cell growth
Jian NIU ; Bin LIU ; Bin YU ; Xiangnong LI
Chinese Journal of General Surgery 2001;0(07):-
Objective To study the effects of lentiviral vector of microRNA targeting IGF1R gene on growth of liver cancer cells.Methods The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed,synthesized and cloned into the pPRIME vector,named pPRIME-IGFIR-miR30-shRNA.The viruses were propagated on 293T cells.Viruses were purified by CsCI gradient according to standard techniques,and functional PFU titers were determined by plaque assay on 293 cells.The effect of pPRIME-IGFIR-miR30-shRNA on IGFIR expression of Hep3B、SMMC7721 cells was detected by RT-PCR and Western blot.The antitumor potential of PRIME-IGFIR-miR30-shRNA to Hep3B、SMMC7721 cells was evaluated by CCK-8 assay and Tunel.Results PRIME-IGFIR-miR30-shRNA was constructed successfully.Functional PFU titers of pPRIME-IGFIR-miR30-shRNA were 4.58?109PFU/ml.PRIME-IGFIR-miR30-shRNA inhibited IGFIR expression and the proliferation of Hep3B、SMMC7721 cells.Conclusions PRIME-IGFIR-miR30-shRNA expressing IGFIR-siRNA can inhibit IGFIR expression and may be used for further investigation of gene therapy of liver cancer.
2.Advances in image-guided intensity modulated conformal radiotherapy of non-small cell lung cancer.
Jun XING ; Jian-Bin LI ; Jin-Ming YU
Chinese Journal of Oncology 2007;29(6):401-404
Carcinoma, Non-Small-Cell Lung
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diagnostic imaging
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radiotherapy
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Humans
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Image Processing, Computer-Assisted
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methods
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Lung Neoplasms
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diagnostic imaging
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radiotherapy
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Radiotherapy Planning, Computer-Assisted
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methods
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Radiotherapy, Intensity-Modulated
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methods
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Tomography, Spiral Computed
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methods
4.Comparison of the clip displacement in the operation cavity during external-beam partial breast irradiation depicted by kV-plain film and cone-beam CT under active breathing control assisted with moderate deep inspiration breath hold..
Cui-cui LIU ; Jian-bin LI ; Jin-ming YU
Chinese Journal of Oncology 2009;31(12):921-922
5.Target volume definition, verification and correction in image-guided conformal intensity-modulated radiotherapy for non-small cell lung cancer.
Ying-Jie ZHANG ; Jian-Bin LI ; Jin-Ming YU
Chinese Journal of Oncology 2008;30(4):241-244
Carcinoma, Non-Small-Cell Lung
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diagnostic imaging
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pathology
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radiotherapy
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Cone-Beam Computed Tomography
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Humans
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Lung Neoplasms
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diagnostic imaging
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pathology
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radiotherapy
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Radiotherapy Planning, Computer-Assisted
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methods
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Radiotherapy, Conformal
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methods
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Radiotherapy, Intensity-Modulated
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methods
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Tomography, X-Ray Computed
8.Comparative study of different screening markers for hemodialysis patients infected with hepatitis C virus
Bin LOU ; Yajun TAN ; Jian FAN ; Jie ZHANG ; Yu CHEN
Chinese Journal of Laboratory Medicine 2009;32(9):993-996
ssay provides a higher detection rate for HCV infection in HD patients.
9.The Value of Neutrophil to Lymphocyte Ratio in Predicting In-stent Restenosis in Patients with Coronary Heart Disease
Liguo ZHANG ; Youming ZHANG ; Bin JIAN ; Zhuo YU
Journal of Kunming Medical University 2013;(8):114-116
Objective To investigate the value of neutrophil to lymphocyte ratio (NLR) in predicting in-stent restenosis (ISR) in patients with coronary heart disease. Methods We collected the data of 618 patients with coronary heart disease hospitalized in the Department of Cardiology in the First Affiliated Hospital of Kunming Medical University from January 2011 to June 2012. All selected patients underwent coronary angiography and stent implantation, and were divided into two groups according to the average size of NLR. The relationship between the neutrophil to lymphocyte ratio and coronary heart disease in-stent restenosis was investigated. Results There were statistically significant differences in the presence of diabetes, high density lipoprotein, low density lipoprotein, the percentage of neutrophils and lymphocytes in patients between two groups (P<0.05) . We found 13 patients (4.2%) and 32 patients (10.4%) with in-stent restenosis in the first group and the second group, respectively, and there was a statistically significant difference between two groups (P<0.01) . Conclusion Neutrophil to lymphocyte ratio is correlated with ISR,and has clinical value in predicting ISR.
10.Automatic printing of hospital imaging film and its report: design and application
Fei XIONG ; Yinshi XU ; Bin CUI ; Haitao YU ; Jian YIN
Chinese Journal of Medical Library and Information Science 2017;26(5):72-74
The problems in traditional imaging film and its reporting process were described.A virtual printing server network and a self-service printing service model of image film and its reporting model,established by improving the traditional imaging film and reporting process,could shorten the waiting time of patients after examinations,reduce the errors in imaging film reports,and improve the working efficiency of staff.