Objective To explore the polymorphism distribution of the hormone-sensitive lipase (HSL) gene i6 in Zunyi region. Methods HSL i6 in the Zunyi population was examined with ploymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and analyzed for its distribution. Results Six different alleles and 11 different genotypes were found in the HSL i6. The PIC is more than 0.7, in which allele 6 and allele 7 were most widely distributed. Conclusion The polymorphism of HSL i6 was identified and the result suggests in Zunyi HSL as a candidate gene of glucose and lipid metabolism.

Objective To explore the polymorphism distribution of the hormone-sensitive lipase (HSL) gene i6 in Zunyi region. Methods HSL i6 in the Zunyi population was examined with ploymerase chain reaction-single strand conformation polymorphism (PCR-SSCP),and analyzed for its distribution. Results Six different alleles and 11 different genotypes were found in the HSL i6. The PIC is more than 0.7,in which allele 6 and allele 7 were most widely distributed.Conclusion The polymorphism of HSL i6 was identified and the result suggests in Zunyi HSL as a candidate gene of glucose and lipid metabolism.

strains that could produce raw starch-digesting glucoamylase were isolated from soil and mildewed rice.The highest raw starch-digesting glucoamylase activity strain named OR-1 was identified as Rhizopus.sp.The raw starch-digesting glucoamylase activity of the strain is 90U/mL.Through UV and NTG mutagenesis,the raw starch-digesting glucoamylase activity raised to 200U/mL and 325U/mL respectively.The RDA were 70% and 65% respectively.

OBJECTIVE:To study the effects of Xiaoruzeng capsule on the gastric acid and pepsin in rats. METHODS:Rats were randomized into a blank group (distilled water),a positive control group (3.6 mg/kg omeprazole ) and the groups of low, middle and high doses of Xiaoruzeng capsule [2.25,4.5,9.1 g(crude drug)/kg]. These groups were respectively marked as groups A,B,C,D and E,with 10 rats in each group. All the rats were given corresponding drugs,ig,for consecutive 10 d. Their suc-cus gastricus was collected 3 h after the last administration,and determined for pH value with precision pH test strip and for free acidity and total acidity by acid-base neutralization titration method. The content and activity of pepsin were determined and calculat-ed with the test kit and microplate reader. The pathological change of the stomach was observed under the electron microscope. RE-SULTS:Compared with group A,groups B,C and D had higher pH value of succus gastricus;groups C,D and E had lower free acidity;groups B,C and D had lower total acidity,group E had higher total acidity;groups B,D and E had lower activity of pep-sin;and group C had higher content of pepsin. Compared with group B,group D had lower pH value of succus gastricus;group C had lower total acidity;group E had higher acidity;and groups C,D and E had higher activity of pepsin. There was statistical sig-nificance(P<0.01 or P<0.05). Gastric mucosal erosive haemorrhage was noted in three rats in group E,and other groups demon-strated no obvious pathological change. CONCLUSIONS:Low dose of Xiaoruzeng capsule can slightly inhibit the gastric acid in rats,but will not effect the activity of pepsin.

Effect of organic acids on the synthesis of 1,3 propanediol was studied.The adsorption of organic acids from glycerol fermentation liquor by ion-exchange resins was investigated.The results showed that organic acid and 1,3 propanediol production was in negative relationship.The static adsorption showed that ion-exchange resin 005 had the best adsorption abilities of the organic acids in the glycerol fermentation liquor.It was showed that the yield of 1,3propanediol increased by 166% after the extraction of organic acids from glycerol fermentation liquor and the convertion rate increased by 34%.

In order to obtain higher L-lactic acid yield industrial strain, the original strain Rhizopus oryzae PW352 was mutated by means of N+ ions implantation and a mutant strain Rhizopus oryzae RE3303 was obtained. Its lactic acid yield was increased by 75% than that of the original one. The acid producing condition was optimized by orthogonal design. The concentration of L-lactic acid reached to 131～136g/L and the conversion rate of glucose was as high as 86%～90% under the optimum condition.

Depression is a serious mental illness with a high incidence. At present, we do not fully understand the specific pathological mechanisms of depression, and the efficacy of drug treatments is very limited. Recent studies have shown that epigenetic changes that occur in specific brain regions may be a key mechanism by which environmental factors to interact with individuals to influence the risk of depression. Therefore, drugs that target epigenetic regulation may become a new direction for the development of antidepressants. Histone deacetylase inhibitors (HDACi) are a class of compounds that inhibit histone deacetylase activity, which has been reported to be associated with depression; this article addresses the use of HDACi in preclinical studies, and their potential therapeutic role and limitations of use in depression.

Objective To study the effect of Porphyra polysaccharide on blood -brain barrier after cerebral ischemia -reperfusion in mice. Methods A total of 180 male ICR mice were randomly divided into sham operation group , model group, control group and low, middle and high dose experimental groups , 30 mice in each group.The transient middle cerebral artery occlusion ( tMCAO) model was established by the method of suture thread in model group , control group and experimental groups.The sham operation group received similar surgical treatment but no line thrombectomy was performed .Control group was intraperitoneally injected 4 mg· kg -1nimodipine before modeling , low, middle and high dose experimental groups were intraperitoneally injected 100, 200, 400 mg· kg -1Porphyra polysaccharide , once a day, continuously for 7 d. Sham group and model group were intraperitoneally injected with normal saline.The cerebral infarct size and brain wa-ter content of the mice in each group were observed .The permeability of the blood -brain barrier was observed by Evans blue ( EB) method.The expressions of matrix metalloproteinase -9 ( MMP -9 ) and zonula occludens -1 (ZO-1) protein in ischemic cerebral cortex were detected by Western blot .Results The volume of cerebral infarc-tion in model group and low, middle, high dose experimental groups were (28.45 ±5.32 )%, (24.73 ±3.65 )%, (22.36 ±4.19 )%, ( 18.61 ±5.72 )%; brain water content were ( 83.23 ±1.56 )%, ( 80.72 ±1.17 )%, (80.38 ±1.32)%, (79.59 ±1.41)%; brain EB content were (8.02 ±0.71), (7.18 ±0.66), (4.75 ±0.73) and (3.93 ±0.64 ) μg· g-1, the levels of MMP-9 protein in ischemic cerebral cortex were 0.65 ±0.09 , 0.52 ±0.11 , 0.39 ±0.08 and 0.31 ±0.07 ; the levels of ZO -1 protein in cerebral cortex were 0.18 ±0.07 , 0.24 ±0.06 , 0.37 ±0.08 and 0.51 ±0.10 , all with significant differences ( all P<0.05 ).Conclusion Porphyra polysaccharide can down-regulate the expression of MMP -9 in brain tissue after cerebral ischemia -reperfusion, up-regulate the expression of ZO -1 and reduce the permeability of blood -brain barrier, which may be one of the mechanisms of Porphyra polysaccharides protecting brain injury after ischemia -reperfusion.

OBJECTIVESTo investigate the techniques of posterior lumbar interbody fusion and pedicle screws in the treatment of degenerative scoliosis and evaluate their clinical significance.

METHODSTwenty five patients with degenerative scoliosis were treated operatively during 1997 - 2002. Among the 25 patients, 13 patients were treated with Brantigan I/F carbon cages in 28 intervertebral spaces, 7 patients were treated with Prospace cages in 15 spaces, 1 patient was treated with CONTACT cages in 2 spaces. The correcting effects, complications and follow-up results were analyzed retrospectively.

RESULTSThe average correction rate of scoliosis was 59% and the rate of pain relief was 88%. There was a case of pseudoarthritis and a case of embolism. During follow-up, correction rate and height of disc spaces were not lost nor shift of interbody cages.

CONCLUSIONPosterior pedicle screws combined with interbody fusion is a safe and effective treatment for degenerative scoliosis.

Adult ; Aged ; Bone Screws ; Female ; Humans ; Internal Fixators ; Male ; Middle Aged ; Retrospective Studies ; Scoliosis ; surgery ; Spinal Fusion

The first hand documents, the process and achievements of Mr. CHENG' s study in Japan in the 1930s were investigated in order to provide basic information to explore the original academic thoughts of Mr. CHENG and the Chengjiang Acupuncture School. It was concluded that the experiences of Mr. CHENG's study in Japan had significant influence on enriching and developing his academic thoughts. It has not only enhanced his confidence in therapeutic effect of moxibustion and revival of Chinese acupuncture, but also established the foundation for the reform of needles and expansion of the domain of Chinese acupuncture and its development after returning home.
Acupuncture ; education ; history ; Acupuncture Therapy ; history ; China ; History, 19th Century ; History, 20th Century ; Humans ; Japan ; Male