Objective: To observe the effects of ginsenoside Rg_(1) , chitosan and TGF-?_(1) on the proliferation and differentiation of human periodontal ligament cells( PDLC). Methods:Human periodontal ligament cells were isolated and cultured. The effects of ginsenoside Rg_(1)(0.01 ?mol/L), chitosan((0.05 g/L,)0.1 g/L, 0.2 g/L) and TGF-?_(1)(0.5 ?g/L, 1 ?g/L, 2 ?g/L) on the proliferative ability of human PDLCs were evaluated with MTT method. The alkaline phosphatase activities of human PDLCs were measured with spectrophotometric assay. The secretion of osteocalcin of human PDLCs were measured with radioimmunological method and the apotosis rates of human PDLCs were assayed with flow cytometry with PI staining method. Results: ①Comparing with the control group, the proliferative ability of human PDLCs in ginsenoside Rg_(1)(0.01 ?mol/L)group ,Chi(0.05 g/L, 0.1 g/L, 0.2 g/L) groups and TGF-?_(1 )((0.5 ?g/L), 1 ?g/L, 2 ?g/L) groups on day 3,5,7 were considerably increased (P

Objective To explore the difference of serum and pleural effusion CEA ,CA125 and CA199 levels in benign and ma‐lignant pleural effusions to accumulate the practical experience for clinical diagnosis .Methods 125 patients with pleural effusion in this hospital from February 2010 to March 2014 were performed the retrospective analysis and divided into the benign group(49 ca‐ses) and malignant group(76 cases) according to the nature of the fluids .The etiology composition ,pleural effusion and serum tumor markers levels were recorded in the two groups and at the same time the diagnosis efficiency of the tumor markers in pleural effusion was calculated .Results The specificity of the combined detection of CEA ,CA125 and CA199 for diagnosing benign and malignant pleural effusion was 90 .6% ,the sensitivity was 95 .1% ,the positive predictive value was 90 .2% ,negative predictive val‐ue was 95 .3% and accuracy was 92 .7% ,which were higher than those of the single index detection and any 2‐marker combined de‐tection .Conclusion The combined diagnosis of CEA ,CA125 and CA199 in pleural effusion conduces to the assisted diagnosis of be‐nign and malignant pleural effusion .

Objective To explore the effect of fluticasone propionate nasal spray(Fu Shu-liang) in improving children's symptoms of allergic rhinitis and recurrent asthma.Methods 100 children with allergic rhinitis and asthma syndrome were randomly divided into control group and experimental group.The control group was treated with loratadine.The experimental group was treated with water-soluble auxiliary Fu Shu-liang nasal spay on the basis of the control group.The treatment effects were observed.Results The total effective rate of the observation group was 94％,which was significantly higher than 76％ of the control group (x2 =6.35,P ＜ 0.05).After 10-12 weeks,the rhinitis symptom score and asthma symptom score between the two groups had statistically significant differences (t =2.47,2.64,3.41 ; 2.30,3.17,2.47,all P ＜ 0.05).The incidence rate of adverse reactions such as nasal dryness,epistaxis between the two groups had statistically significant differences (x2 =7.11,7.53,all P ＜ 0.05).Conclusion Fu Shu-liang(Eph propionate and fluticasone propionate nasal spray) has good effect in improving symptoms of children with allergic rhinitis and asthma,it is worthy of clinical vigorously promoted.

Objective To analyze the pathophysiological variation in patients with penetrating cardiac trauma (PCT) and discuss the classified treatment strategies.Methods A retrospective analysis was conducted on pathogenetic condition,medical interventions and other clinical information of 133 PCT patients.The patients were divided into four groups in accordance with their symptom classification on arrival at emergency department,including sub-clinic type,cardiac tamponade type,hemorrhagic shock type and agonal type.Meanwhile,the patients were classified into three groups according to their pathogenesis phase,including sub-clinical phase,clinical phase and agonal phase.Development characteristic of patients' condition in each group was investigated in each time point by survival analysis.Impacts of medical interventions on prognosis of those patients were analyzed,such as pericardial space exploration,emergency room thoracotomy (ERT),operating room thoracotomy (ORT).Results Some patients at sub-clinical phase were aggravated into clinical phase or agonal phase,as well as some patients at clinical phase were aggravated into agonal phase during in-hospital treatment.There were significance differences of posttraumatic suvival course among the four groups,namely sub-clinical type,cardiac tamponade type,hemorrhagic shock type and agonal type (P ＜ 0.01).The differences of posttraumatic survival course were also significant among the three groups,namely sub-clinical phase,clinical phase and agonal phase (P ＜ 0.01).Conclusion Clinical symptom classification (or pathogenesis phase) of PCT may not be always unchangeable,thus it is recommended that PCT patients should be treated based on their clinical symptom classification or patbogenesis phase at consultation.

Acute Disease ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Nitrites ; poisoning

Ehrlich's ascites cancer (EAC) in BALB/C mice by ip transplantable EAC cell is reproduced by us. The change of serum malondialdehyde(MDA), plasma cellular cAMP and adenylate cyclase (AC) are examined in the early, middle and late phase of mice bearing EAC respectively in comparison with that of control group. Results indicate that serum MDA in mice bearing EAC is continuously increased with the progress of the disease in the remarked ways in comparison with the control group. Whereas the plasma cAMP in the mice bearing EAC is markedly decreased in that order. Relation between the change of serum MDA and the cellular cAMP, AC or plasma cAMP shows a significant negative correlation efficiency (P

Objective To explore the cause and epidemiologic characteristics of Keshan disease.Methods The cases observation method,epidemiologic methods,fungus-mycotoxin methods and the analytic chemistry methods were used in our studies.Results From the year 1973-1975,in serious endemic areas,fifteen families and one disease outbreak village were investigated,it was found that all patients and his family members had eaten a vast amount of mould millet or maize 4-12 months before onset of the disease; the millet samples collected from endemic areas were cultured and they werc found to be polluted mainly by fungus(20％ of all),the toxin was citreoviridin(CIT).The cause factor of Keshan disease entered into human body through grain but not drinking water,and the transmitting effect was not different with different grains,millet and maize transmit but wheat did not;according to data of endemic disease inspection,after 1990 no new cases of Keshan disease had been reported in the country,at the same time the CIT content in grain produced in endemic areas did not reduced so quickly.However,after 1983,the reform of agriculture promoted significantly the hygienic quality of grain which was considered to be a specific cause for the decreasing of Keshan disease threatening; Keshan disease was distributed on the narrow-long areas from Yunnan till Heilongjiang.These areas were the connection parts,between the cold west north high lands and the warm-moist east-south flatlands,a narrow-long region.The year mean temperature and humidity in endemic areas might be suitable for growing of fungus and production of its toxin ; of animal experiment,rats were feed with CIT contaminated grain every day 4 mg/kg(body weight) for 4-6 weeks,the necrosis of myocardium and cell mitochondria swelling,proliferation and damage were found,just like what had been seen in died cases of Keshan disease.Conclusions The cause of Keshan disease is CIT toxin,the endemic conditions are geographic climate suitable for growing of fungus and production of its toxin and bad hygienic situation of grain storage.Some discussion about the prevention mcthod was proceeding.

Objective To understand the distribution of pathogen and antibiotic resistance of nosocomial fungal infection,and provide evidence for the prevention of fungal infection in premature infants in hospital.Methods The clinical data of 72 cases of premature infants with nosocomial fungal infections were retrospectively analyzed.Results The top three of the pathogenic of fungal infection in premature infants in our hospital were 38 strains of Candida pelliculosa (52.8％,38/72),10 strains of Candida albicans (13.9％,10/72),9 strains of Candida parapsilosis (12.5％,9/72).The weights of 72 cases with nosocomial fungal infection in preterm infants were below 2000 g,who were dominated by very low birth weight infants.Gestational age of 27 ～ 30 weeks were in the majority,which accounted for 36.1％ of 27 ～ 28 weeks (26/72) and 34.7％ of 29 ～30 weeks (25/72),each group were dominated by bacteria pathogen candida.Seventy-two strains of fungi were isolated to amphotericin B drug resistance,the 1.4％ (1/72) resistance rate to fluconazole,4.2％ (3/72) pairs of voriconazole resistance rate,2.8％ (2/72) for itraconazole resistant rate.There were no significant differences among the last three resistant rate (x2 =1.02,P ＞ 0.05).The 5-fluorine cytosine resistance accounted for 59.7％ (43/72),and the 4 kinds of drug resistance rate had significant difference (x2 =57.73,P ＜ 0.05).There was no significant difference in resistance rate between amphotericin B and fluconazole (x2 =1.01,P ＞ 0.05).Conclusion Fluconazole could be used to those premature infants with high risk factors of fungal infection.Once the fungal infection is diagnosed,intravenous fluconazole could be the first choice,when necessary,amphotericin B can be used together.

Objective: To investigate the root canal configuration and the incidence and location of the isthmus in extracted maxillary second premolar with single root. Methods: 60 maxillary second premolars were included in the study. 1 mm root was cut from the apical end of root. The transverse section was stained with methylene blue dye and viewed with a surgical operating microscope. 1 mm increment sections were made and stained and observed by the same way from apex to 6 mm. The number of canals as well as the type of canal isthmuses were recorded. Results: 41.67% maxillary second premolar with single root had two canals. Canal isthmus happened more frequently in the apical 4 mm levels. Conclusion: The incidence of isthmus in the maxillary second premolar was highest in the apical 3-to 5-mm level. Canal morphology in the apical 1-to 6-mm is complicated.

Traditional Chinese medicine emphasizes the overall state of health, pays attention to individual health condition, highlights maintaining health rather than diseases, shows the clinical effectiveness with flexible treatment. It is the unique resource of health service in our country. Currently, the qualification and certification of traditional Chinese medicinewere only applied in medical practitioners of traditional Chinese medicine and Gua sha.This led to the status quo of vacuum to the traditional Chinese medicine in the fields of access system and standardization. Thus, it is urgent to unify and standardize the access and qualification traditional Chinese medicine, in order to TCM inheritance and development. The establishment of the system should be based on clinical effect and difference among the clinical application. The system can be divided into easy, middle and senior level TCM therapist. TCM therapist with different levels can apply the qualification and certification based on their own levels.