To observe the levels of platelet-activating factor (PAF), interleukin-6 (IL-6) and IL-17 in peripheral blood of rats with adjuvant arthritis (AA), and the effects of Xinfeng Capsule (XFC), a compound traditional Chinese herbal medicine.

Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional ; Proteome

Objective To observe effects of Xinfeng capsule (XFC) on platelet parameters,ultrastructure,p-selectin and therapeutic effect on rheumatoid arthritis (RA) patients in active phase.Methods Platelet parameters,p-selectin and reactive indexes of 60 RA patients in active phase were detected.RA patients were divided into XFC treated group (35 examples) and Zhengqing Fengtongning (ZQF) control group (25 examples) randomly.After a course of treatment,the therapeutic effect and changes of platelet parameters,p-selectin,reactive indexes of two groups were observed.Setting up a normal control (20 examples) and detecting above-mentioned indexes.Observing platelet ultrastructure of two examples with transmission electron microscope of per groups.Results Compared with normal control group,PLT,PCT,MPV,CD62P of RA patients in active phase was obviously increased.Platelet ultrastructure was obviously damaged.The excellence rate of XFC exceeded ZQF control group.After being treated,platelet ultrastructure of the two groups were obviously improved,and XFC group was better than ZQF group.Compared with ZQF group,PLT,PCT,CD62P were obviously decreased in XFC group.PLT,PCT were positively correlated with CD62P,IgG,ESR,CRP,RF,arthrpain,arthrocele.And PLT was positively correlated with arthrtenderness,morning stiffness time.Conclusion PLT and PCT of RA patients in active phase were increased,and have correlation with reactive indexes.It suggests that PLT,PCT can reflect reactiveness of RA as objective indexes.XFC can not only treat inflammation and delay the aggravation of RA patient by improving platelet ultrastructure.

OBJECTIVE: To observe the effects of Xinfeng Capsule (XFC), a compound Chinese herbal medicine, on behaviors and myocardial ultrastructure in adjuvant arthritis (AA) rats. METHODS: Fifty-five rats were randomly divided into normal control group, untreated group, methotrexate (MTX) group, Tripterygium wilfordii glycosides tablet (TGT) group and XFC group. There were 11 rats in each group. Except for those in the normal control group, the rats in the other groups were all intracutaneously given 0.1 ml Freund's complete adjuvant in the right hind limb. Changes of behaviors and myocardial ultrastructure of the rats in different groups were observed. RESULTS: Voix pedis' swelling and arthritis index in XFC, MTX and TPT groups were all obviously improved as compared with the untreated group. After XFC treatment, the autonomic activities were obviously increased, number of errors in exercise period and test period were obviously decreased, step-down latency (SDL) was lengthened and escape latency (EL) was shortened. Overall structure of myocardial myofibril and the majority of cristae was intact in XFC group. The effects of XFC in improving the behaviors and myocardial ultrastructure were better than those of MTX and TPT. CONCLUSION: Changes of behaviour and myocardial ultrastructure of AA rats can be observed. XFC can improve the myocardial ultrastructure of AA rats as well as their behaviors.

APOBEC3 is a class of cytidine deaminase, which is considered as a new member of the innate immune system, and APOBEC3G belongs to this family. The research about APOBEC3G is a new direction of innate immune defense mechanism against virus. APOBEC3G has the restrictive activity on many viral replications, which deaminates dC to dU in the viral genome and then induces extensive hypermutation. APOBEC3G can also interrupt viral replication at several phases such as reverse transcription, replication, nucleocapsid and so on by non-deamination mechanisms. However, virus can encode viral proteins to counteract the restriction activity of APOBEC3G. Elucidation of the antagonistic interaction between APOBEC3G and the virus will be contributed to development of new antiviral drugs in the future.
APOBEC-3G Deaminase ; Animals ; Cytidine Deaminase ; genetics ; metabolism ; DNA Replication ; Deamination ; HIV-1 ; physiology ; Hepacivirus ; genetics ; physiology ; Hepatitis B virus ; genetics ; physiology ; Humans ; Paramyxoviridae ; genetics ; physiology ; Retroviridae ; physiology ; Virus Replication ; vif Gene Products, Human Immunodeficiency Virus ; metabolism

Adolescent ; Athletes ; Cortisone ; blood ; Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Hemoglobins ; analysis ; Humans ; Male ; Medicine, Chinese Traditional ; Phytotherapy ; Testosterone ; blood

Air Pollutants, Occupational ; analysis ; Chromatography ; methods ; Sulfur Dioxide ; analysis ; Workplace

Calcinosis ; Female ; Humans ; Infant, Newborn ; Vascular Diseases ; pathology

Objective:To observe effects of Xinfeng Capsule(XFC)on platelet parameters(platelet count,thrombocytocrit,mean platelet volume,platelet distribution width),p-select,platelet ultrastructure and its therapeutic effect on rheumatoid arthritis(RA)patients in active phase.Methods:To detect platelet parameters,p-select and reactive indexes(ESR,?1-AGP,CRP,RF)of 60 RA patients in active phase.Patients were divided into XFC treated group(35 examples),Zhengqingfengtongning(ZQF)control group(25 examples)according to random digits table.After a course of treatment,to observe therapeutic effect and the changes of platelet parameters,p-select,reactive indexes of two groups.Setting up a normal control(20 examples)and detecting above-mentioned indexes.To observe platelet ultrastructure with transmission electron microscope.Results:1.Compared with normal control group,PLT,PCT,MPV,Ps of RA patients in active phase increased obviously(P0.05).But the excellence rate of XFC exceeded that of control group(P

Objective To investigate the effect of vaccum-assisted closure(VAC)on the expression of C-ski and Smad3 in the human wound margin tissue and to explore their significance in triggering the wound healing.MethodsFourteen patients,9 males and 5 females with chronic wound admitted in our department from April 2007 to April 2009 were enrolled into the study after signed an informed consent,and the full-thickness skin defects were collected before treatment,and 1,4 or 7 d after treatment.Immunohistochemistry(IHC)was carried out to detect the expressions of C-ski and Smad3,and RT-PCR was used to determine their mRNA expressions of C-ski and Smad3.ResultsIHC results showed that there existed little C-ski in the wound margin tissue before treatment,and after treatment,it were gradually increased.Compared with before treatment group,the differences in 4 and 7 d after treatment were markedly significant(P0.05).Compared with above groups,the differences in 4 and 7 d after treatment groups were markedly significant(P