Objective To study the gene expression patterns in human lung squamous cell carcinoma and adenocarcionma with cDNA chip.Methods The mRNA was extracted from cancer tissue and normal lung tissue,and then was labeled by Cy5-dCTP or Cy3-dCTP.The mixed probes were hybridized to cDNA chip containing 4096 genes and to study the gene expression patterns in lung squamous cell carcinoma and adenocarcionma.Results Seventeen genes were up-regulated and 19 genes were down-regulated in both lung squamous cell carcinoma and adenocarcinoma,the expression of 20 genes were remarkable higher in squamous cell carcinoma than that in adenocarcinoma and the expression of 14 genes were remarkable lower in squamous cell carcinoma than that in adenocarcinoma.Conclusion Many genes are possibly involved in the initiation and progression of human lung cancer;cDNA chip technique might be a useful method in screening lung cancer implicated genes.

Objective:To discuss management and use for electrocardiogram (ECG) monitor during detection and repairing.Methods: To solve the problems in the process of management and use of electrocardiogram (ECG) monitor through detection and repairing of this equipment, and will discuss the management measures of ECG monitor. Furthermore, the effective quality control measures for ECG monitor will be raised.Results: Qualified rate of ECG monitor is 80.47%.It is useful to improve measure of quality control by PDCA.Conclusion: Quality control will improve the utilization rate of medical resources, and reduce investment costs of these resources.

Recently,with the recognition of blood supply and anatomy of the liver caudate lobe,the intervention therapies of hepatocellular carcinoma in caudate lobe were enriched.However,because of the complex anatomy,the treatment of hepatocellular carcinoma in caudate lobe is still controversial.The interventional methods for hepatocellular carcinoma in caudate lobe were mainly included transcatheter arterial chemoembolization (TACE),percutaneous ethanol injection (PEI),transcatheter arterial embolization (TAE),radioembolization,radiofrequency ablation (RFA) and endoscopic ultrasound-guided ethanol injection.The present statues and progresses of interventional therapy of hepatocellular carcinoma in caudate lobe were reviewed in this article.

Acupuncture Therapy ; Adult ; Alcoholism ; therapy ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Peripheral Nervous System Diseases ; therapy

OBJECTIVE: To investigate the progress of clinical application of implant during distraction osteogenesis in the jaw bones, and to evaluate its biocompatibility.METHODS: The first author retrieved CNKI database from 1989 to 2009 in Chinese and Medline database from 1989 to 2009 in English with the key words of "jaw, distraction osteogenesis, treatment". A total of 56 articles were screened according to inclusion and exclusion criteria. Finally, 20 articles concerning treatment progress of distraction osteogenesis and clinical application of implant were included.RESULTS: Distraction osteogenesis implants contained intraoral or extraoral distraction device, which could be used for distraction of transport plate. A distraction was selected in accordance with patients' condition and requirement. Distraction osteogenesis had been an important manner for complicated tooth jaw facial deformity and jaw defect in oral dentofacial surgery and Orthodontic treatment. It could have effective osteogenesis in combination with various systemic therapy, local therapy and physical therapy. Presently, distraction osteogenesis implants were metal materials. Metal implants have great advantages in preventing bacterium growth, keeping implant fixation and distraction effects, and the price was high. However, the cost of Ni-Ti memory alloy wire was lower compared with common retractors. The fixing device and alloy wire closely adhered to the bone could be completely implanted into tissues, with the advantages of anti-infection and completely closing the exterior and interior wound. CONCLUSION: Distraction osteogenesis is a new method for treating dental and craniofacial hypodevelopment straightening of teeth and for repairing jaw bone defect deformation. Biocompatibility of metal implants was good such as internal small Ni-Ti memory alloy wire. The biocompatibility of other types of implants should be improved.

Some human leucocyte antigens(HLA),espeually class Ⅱ genotypes such as HLA DQ/DR are closely linked to type 1A diabetes mellitus(T1ADM).But the genetic susceptibility of HLA DR/DQ are different between Caucasian and Asian populations.The aspartic acid at position 57 of DQ? can not confer resistance to T1ADM in Asians.DQ2 and DQ8 are the well-known susceptible genotypes in Caucasians while DQ4 and DQ9 are in Asians.An unexpected high risk of T1ADM is observed for the linkage disequilibrium between DRB1*0301/ DRB1*0401 and DQ2/DQ8 in both the populations.The DQB1*0302,DQA1*0501,DRB1*0401,*0402,*0407 are associated with T1ADM in Caucasians,but it is not the case in Asians.In contrast,DQB1*0401 shows a positive association to T1ADM in Asians,has a protective effect against T1ADM in Caucasians.Additionally DRB1*09 could be another susceptible gene in Asians.

BACKGROUND: Drug therapy can improve the clinical symptoms and extend the gestational age through the maternal way to treat intrahepatic cholestasis of pregnancy (ICP), however, fetal hypoxia and meconium-stained amniotic fluid can not clearly improved. OBJECTIVE: To explore the effect of replacement of amniotic fluid that contains high concentrations of bile acids by artificial amniotic fluid through amniocentesis in the rat model of ICP. DESIGN, TIME AND SETTING: Random control animal experiments were completed in the Laboratory (first-degree laboratory) of Animal Experimental Center in Chongqing Medical University from March to September 2008. MATERIALS: Forty SD pregnant rats with 15 days of pregnancy were randomly divided into intracavitary injection of S-Adenosyl-L-methionine (SAMe) (IUS), intracavitary injection of sodium chloride (IUN), intravenous injection of SAMe (IVS) and blank control (IC) groups, with 10 rats in each group. METHODS: On day 15 of pregnancy, ICP rat models were induced by daily injection of estradiol benzoate. On day 17 of pregnancy, 1 mg SAMe and 0.3 mL sodium chloride were injected into amniotic cavity of in the IUS and IUN groups through amniocentasis. Meanwhile, 30 mg SAMe were injected though tail veins in IVS group. No treatment was performed in the IC group. MAIN OUTCOME MEASURES: The meconium-stained amniotic fluid rate and the still birth rate were calculated. Total bile acid (TBA) level in amniotic fluid was measured. In addition, the expression of hypoxia-inducible factor 1 ?(HIF-1?) mRNA in the placenta of rats were examined by immunohistochemistry and reverse transcription PCR at the days 20 of pregnancy. RESULTS: The meconium-stained amniotic fluid rate in the experimental group was lower than that of the IC group (P

Objective To study the growth inhibision effect of disodium cantharidinate and Vitamin B6 injection on hepatocellular carcinoma cell line HepG2.Methods Different concentrations of disodium cantharidinate and Vitamin B6 injection were applied on the hepatocellular carcinoma cell line HepG2.The inhibition rate of HepG2 growth was measured by MTT assay.The apoptotic rate was detected with flow cytometry,and the morphology of apoptosis was observed by fluorescence microscope.Results The growth of HepG2 cells was significantly inhibited as well as the apoptosis of HepG2 cells was significantly encouraged by disodium cantharidinate and Vitamin B6 injection(P

Objective To evaluate the efficacy of D98A,a newly developed superparamagnetic oral MRI contrast agent,in the diagnosis of abdomen.Methods Plain and contrast MRI after taking 500～1000 ml D98Ain 80 patients were performed by using different field strength MR scanners,and the sequences included T 1WI,T 2WI,and fat suppressed sequences.The contrast effect,distribution,influence on demonstration of contours of abdominal organs,and side effects were studied concerning this suspension.Results It showed that this oral contrast agent had good security character,wide consideration area,low cost,good contrast effect,and high value of clinical application.The gastrointestinal tracts appeared as hypointensity on various MR sequences,and thus,made them easy to be identified.The margins of abdominal organs were easier to be located after taking the contrast,and the distinction had statistical denotation.The demonstration rate of gastrointestinal tracts was raised from 10% to 89% after using D98A.There were no side-effects in all patients. Conclusion D98A is safe and effective in facilitating the localization of the abdominal organs.It can improve the accuracy in delineating the gastrointestinal tracts and in diagnosing the lesions of abdominal organs.

Objective To investigate the etiology of the nosocomial infection and immune function changes anus and anal after operation.Methods 130 patients who needed anus and anal canal surgery were recruited in the study.The clinical data were recor-ded and postoperative nosocomial infection data were observed,including infection type,distribution,pathogenic analysis and im-mune function.Results A total of 67 cases of nosocomial infection occured,the infection rate was 51.5% (67/130),the main types of infection were perineal wound infection,abdominal infections and pelvic infections,urinary tract and vaginal infections were rare. 93 isolates were collected,of which gram-negative bacilli accounted for 67.7% (63/93 ),including bacteroides fragilis (21.5%), Escherichia coli (18.3%),Pseudomonas aeruginosa (10.8%);Gram-positive bacteria accounted for 23.7% (30/93 ),including Staphylococcus aureus(17.2%),Staphylococcus epidermidis(6.5%);5 fungi were isolated accounting for 5.4%,other strains ac-counted for 3.2%.All the immune parameters detected in patients with postoperative nosocomial infection were statistically differ-ent from those before infection(P <0.05)except for IgM(P >0.05).Conclusion The incidence of nosocomial infection was rela-tively high in patients who had anus and anal canal surgery,which could serious affect the patients'outcomes,preventive and control measures should be taken in clinical practice.