The effect of traditional therapy is limited for liver cancer,gene therapy gets more and more recognition in recent years.Oncolysis virus is a kind of conditionally replicating virus,with special reproductivity in cancer cells,and then kills them.Gene agents are usually introduced into tumor tissue by intra-tumor and intra-arterial injection,and the technique of interventional therapy is able to satisfy the demand excellently.So,some breakthrough is expected in treating liver cancer by skillfully combining oncolysis virus and interventional technique

CCAAT-Enhancer-Binding Proteins ; genetics ; Cytogenetic Analysis ; Cytogenetics ; methods ; Extremities ; Humans ; Liposarcoma ; etiology ; genetics ; Liposarcoma, Myxoid ; etiology ; genetics ; Molecular Biology ; methods ; Oncogene Proteins, Fusion ; genetics ; RNA-Binding Protein FUS ; genetics ; Retroperitoneal Neoplasms ; etiology ; genetics ; Ring Chromosomes ; Soft Tissue Neoplasms ; etiology ; genetics ; Transcription Factor CHOP ; Translocation, Genetic

Twenty-nine thyroid tissue samples were collected from patients with thyroid nodules.The total RNA were extracted,the gene expressions of TFF3,HMGA2,C1orf24,and DDIT3 were detected by RT-PCR.In comparison with normal thyroid tissues,the expression of C1orf24 mRNA was decreased in the follicular thyroid adenoma (FTA) group,but increased in the follicular thyroid carcinomas (FTC) group.The expressions of DDIT3 and HMGA2 mRNA were increased in both FTA and FTC groups,and were even higher in the latter.The expressions of TFF3 mRNA level were decreased in FTA and FTC.The data suggested that molecular markers C1ort24,DDIT3,HMGA2,and TFF3 in thyroid tissue seem to be helpful in the differential diagnosis between follicular adenomas and carcinomas.

To investigate the expression of inducible nitric oxide synthase (iNOS) in the hydropic cochlea of guinea pigs, the experiment consisted of two groups: normal control group and experimental group. The hydropic animal model was established by destroying the endolymphatic sac. The expression of iNOS in the hydropic cochlea was examined by immunohistochemistry. The results showed that the expression of iNOS in the hydropic cochlea was positive. The expression of iNOS in the area of stria vascularis and spiral ganglion cells was much stronger. It is suggested that nitric oxide is involved in the pathophysiology of cochlear dysfunction in hydropic cochlea.

The object of this study was to investigate the anti-ototoxic effect of iminoethyl-lysine on cochlea of guinea pig damaged by gen-tamicin. The experiment consisted of three groups: normal control group, experimental treatment control group and experimental treatment group. Guinea pigs of the latter two groups were subjected to hypodermic injection of gentamicin, at the same time guinea pigs of the experimental treatment group were treated with intraperitoneal injection of iminoethyl-lysine. while guinea pigs of the experimental control group were treated with the same volume of saline. Guinea pigs of the normal control group were treated with hypodermic and intraperitoneal injection of the same volume of saline as that of the other two groups. The expression of iNOS was examined in the cochleas of all groups by immunohisto-chemistry. ABR hearing threshold shift were examined, and the damage of hair cells of cochleas of all groups was investigated by scanning electronic microscopy. The results showed that iNOS expression was negative in cochleas of the normal control group, and positive in that of other two groups. The expression of iNOS in the experimental control group was much more stronger than that of the experimental treatment group. The ABR threshold shift of the experimental treatment group was much more lower than that of the experimental control group. The damage of cochlea of the experimental control group was much more severe than that of the experimental treatment group. These results suggest that the expression of iNOS is positive in gentamicin-damaged cochleas. Iminoethyl-lysine has significant anti-ototoxic effect on the cochlea damaged by gentamicin, suggesting nitric oxide plays a very important part in the process of the cochlea injury by gentamicin.

Adolescent ; Adult ; CD57 Antigens ; metabolism ; Diagnosis, Differential ; Female ; Glial Fibrillary Acidic Protein ; metabolism ; Humans ; Male ; Middle Aged ; Neurilemmoma ; metabolism ; pathology ; Pelvic Neoplasms ; metabolism ; pathology ; Peripheral Nervous System Neoplasms ; metabolism ; pathology ; Retroperitoneal Neoplasms ; metabolism ; pathology ; S100 Proteins ; metabolism

Tibetan medicineLiu-Wei Mu-Xiang(LWMX) pill, a traditional compound medicine, has special therapeutic effects in the treatment of gastrointestinal anabrosis, especially chronic gastric ulcer. It has been widely applied in the clinics in Tibetan region. Nevertheless, the current standards for quality control are insufficient and incomplete, which makes it difficult to ensure its quality. This review focused on two closely related perspectives, which were the effective material basis and quality control, to summarize and review the recent advancement in relevant studies. This review also pointed out that the burning questions for Tibetan medicine standards were the origin confusion and“different medicines with the same name”. It offered meaningful suggestions for the consideration to improve its quality standards.

Classification ; methods ; Dermatofibrosarcoma ; classification ; pathology ; Gastrointestinal Stromal Tumors ; immunology ; pathology ; Hemangioendothelioma ; classification ; metabolism ; pathology ; Humans ; Neoplasms, Connective and Soft Tissue ; classification ; metabolism ; pathology ; Neurilemmoma ; pathology ; Rhabdomyosarcoma ; metabolism ; pathology ; Sclerosis ; World Health Organization

Key trial activities include: development of the trial protocol;development of standard operating procedures;development of support systems and tools;generation and approval of trial information documents;selection of trial sites and the selection of properly qualified,trained,and experienced investigators and study personnel;ethics committee review and approval of the protocol;review and approval by applicable regulatory authorities;enrollment of subjects into the study: recruitment,eligibility,and informed consent;the investigational product(s): quality,handling,and accounting;trial data acquisition: conducting the trial;trial data acquisition: conducting the trial; safety management and reporting;monitoring the trial;managing trial data;quality assurance of the trial performance and data;reporting the trial.

Adult ; Base Sequence ; DNA, Neoplasm ; genetics ; Exons ; Female ; Humans ; Liposarcoma ; genetics ; Liposarcoma, Myxoid ; genetics ; Male ; Middle Aged ; Molecular Sequence Data ; Oncogene Proteins, Fusion ; genetics ; Polymerase Chain Reaction ; methods ; RNA-Binding Protein EWS ; genetics ; RNA-Binding Protein FUS ; genetics ; Sequence Analysis, DNA ; Transcription Factor CHOP ; genetics ; Translocation, Genetic