Abdominal Wall ; pathology ; surgery ; Hernia, Abdominal ; etiology ; surgery ; Humans ; Models, Anatomic ; Postoperative Complications ; etiology ; surgery

Enterostomy ; adverse effects ; methods ; Hernia, Ventral ; etiology ; surgery ; Humans ; Postoperative Complications ; etiology ; surgery

Anastomosis, Surgical ; adverse effects ; Brachiocephalic Trunk ; pathology ; Hemorrhage ; etiology ; therapy ; Humans ; Larynx ; surgery ; Male ; Middle Aged ; Postoperative Complications ; Trachea ; surgery ; Tracheal Stenosis ; etiology ; surgery ; Tracheotomy ; adverse effects

Objective The study was to investigate the relationship among angiotensin 1-converting enzyme(ACE), plasminogen activator inhibitor-1 (PAI-1)gene polymorphisms and the common carotid artery (CCA-IMT), and the predicting effects of them on CCA-IMT in newly diagnosed type 2 diabetes (T2DM). Methods The polymorphisms of ACE (I/D) gene and PAI-I (4G/5G) gene were deter-mined by polymemse chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele-specific polymerase chain reaction (AS-PCR) method in 308 cases with T2DM. CCA-IMT was compared among the groups with different genotypes of ACE and PAI-1. The in-dependent or synergistic effects of the ACE I/D and PAI-1 40/5G polymorphisms on CCA-IMT in 308 patients with T2DM were analyzed with multivariate linear regression. Then the 156 newly diagnosed type 2 diabetics (durations< I year) without AS received the maltifactorial targeted intervention, including taking aspirin and controlling blood glucose, blood pressure, blood lipid and body weight. The differences of metabolic control, ACE (I/D) and PAId (40/5G) gene polymorphisms were analyzed. Logistic regression analysis was used to analyze the eorrelation among the CCA-IMT, ACE (I/D) and PAI-1 (4G/5G) polymorphisms. Results Patients with ACE DD genotypes had higher CCA-IMT than those with ACE-Ⅱ or ACE ID genotypes. Patients with both ACE DD and PAI-1 404G genotypes had a higher CCA-IMT than those with any other pairs of genotypes. Multivariate linear regression analysis showed that ACE DD and PAI-1 4G4G gene polymorphisms had synergistic effect on the CCA-IMT in T2DM patients. After 2 years multifactorial intervention, the frequencies of PAI-1 4G alleles and 404G genotypas were lower than those in the CCA-IMT non-inereasing group. Conclusions These findings indicate that the ACE-DD geno-type and its synergistic effects with the PAI-1 4G/4G genotype are independent risk factors for the CCA-IMT in T2DM patients. Under multi-factorial intervention for 2 years, PAI-1 4G/4G genotype may be a negative predictor for the progression of CCA-IMT in T2DM patients.

?ig-h_3 was first identified as a transforming growth factor-beta1-inducible gene in human lung adenocarcinoma cell line. It encodes for a secreted extracellular matrix (ECM) protein, which is thought to act on cell attachment and ECM composition. Previous study showed that ?ig-h_3 were highly expressed in human hepatoma cell lines and lowly expressed in human normal hepatic cells. The present study aimed to transfect ?ig-h_3 into 7721 cells to investigate its effect on secretion of MMPs in the transfected human hepatoma cells. Full-length ?ig-h_3 gene,cloned by reverse transcription polymerase chain reaction (RT-PCR) was inserted into the eukaryotic expression vector pEGFP-C_2. The recombinant plasmid was transfected into 7721 cells with Lipofectamine2000 and Gelatin-Zymography were adopted to detect the production of MMPs in the transfected cells. Results showed that ?ig-h_3/pEGFP-C_2 recombinant expression plasmid was successfully constructed and achieved high transfection efficiency. MMPs expression of the transfected cells was promoted significantly. These results suggest that overexpression of ?ig-h_3 promoted the production of MMPs, indicating that ?ig-h_3 may play roles in the invasive and metastatic processes of hepatoma.

Objective To evaluate the value of neutrophil gelatinase - associated lipocalin (NGAL) for diagnosis of acute kidney injury (AKI) after cardiopulmonary bypass established. Method 120 children undergoing cardiopulmonary bypass were enrolled in this prospectively study. Serial urine samples were analyzed by ELISA for NGAL expression. The primary outcome measure was AKI, which was defined as a 50% or greater increase in serum creatinine from baseline. Result 21 children ( 17.5% ) developed AKI. Urine concentrations of NGAL increased after cardiopulmonary bypass had been established for 2 hours. The concentration of NGAL in urine after cardiopulmonary bypass had been established for 2 hours was used to diagnose AKI. The area under the receiver-operating characteristic curve was 0. 96, the sensitivity was 92%, and the specificity was 91% for the cutoff value of 100ng/mg Cr. Conclusion Concentrations of NGAL in urine might be used as a sensitive and specific biomarker for early prediction of AKI after cardiopulmonary bypass had been established.

Objective To compare the perioperative outcomes and long-term therapeutic response of laparoscopic splenectomy versus open splenectomy for idiopathic thrombocytopenic purpura.Methods A retrospective analysis of 124 patients who under-went splenectomy(68 LS and 56 OS)for ITP between January 2011 and January 2015 was conducted.Results Patients undergoing LS were found to require a longer operative time(P <0.05 )but had reduced hospital stay,lower intra-operative blood loss(P <0.05),less postoperative pain,earlier drain removal,and decreased incidence of complications(P <0.05).Conversion to OS was re-quired in 4 patients for excessive loss of blood(5.8%).Deep venous thrombosis(DVT)was observed in 1 patients after OS.One pa-tient died from pneumonia after LS.Mean follow-up of (33±11.8)months was performed in LS group and of (32±12.9)months in OS group.50 patients(73.5%)in LS group and 43(76.7%)in OS group reached sustained complete haematological response(P >0.05).Kaplan-Meier analysis showed that there was no significant difference in the relapse-free survival rate between the groups (P =0.679).Conclusion Compared with open splenectomy,laparoscopic splenectomy for patients with ITP has similar long-term therapeutic response,but it has advantages of minimally invasiveness.

Objective To compare the effect of three methods in diagnosis of plague by detecting of Yersina pestis F1 antigen. Methods In natural foci of plague, wild animal samples, such as blood, liver, spleen,and lymphoid tissue were collected, and the three methods of enzyme linked immunosorbent assay (ELISA),reverse indirect hemagglutination assay(RIHA) and gold-immunochromatography assay(GICA) were employed to detect F1 antigen of Yersina pestis. Results Total of 414 infused organ samples of natural death and captured wild animals in natural foci of plague were determined. Positive samples detected by GICA and ELISA were the same,the positive rates were 5.31%(22/414), both positive and negative coincidence rates were consistently 100%. Only 18 samples were positive by retrial in 186 samples with more than 2 holes aggregation by preliminary examination of RIHA, with nonspecific agglutination rate of 40.6% (168/414) and positive rate of 4.35% (18/414). The positive coincidence rate was 81.82% (18/22) between RIHA with GICA and ELISA, and negative coincidence rate was statistically significant(t = 4.379, P ＜ 0.01). Conclusions ELISA, RIHA and GICA can be used for early diagnosis of plague by detecting F1 antigen. The results of RIHA have quantitative significance, with higher non-specific agglutination rate, and heavy workload of re-examination; GICA and ELISA has the same specificity and sensitivity, but the results of GICA is only qualitative. ELISA excluded the defect of RIHA and GICA, and combines the advantages of both methods.

Adolescent ; Adult ; Aged ; DNA, Viral ; Female ; Hepatitis B Core Antigens ; genetics ; Hepatitis B virus ; genetics ; Hepatitis B, Chronic ; genetics ; Humans ; Male ; Middle Aged ; Mutation ; Young Adult

Objective To evaluate the efficacy of corneal bandage contact lens or amniotic membrane in the treatment of pterygium.Methods A total of 114 patients (114 eyes) with primary pterygium treated in our hospital from April 2015 to December 2016 were randomly divided into amniotic membrane group (61 patients) and bandage contact lens group (53 patients).All patients underwent local anesthesia with pterygium excision combined with autologous free conjunctival flap transplantation by the same surgeon.In the amniotic membrane group,10 mm × 15 mm lyophilized biological amuiotic membrane was used,and its basal plane was applied to cover the keratoconjunctival wound of the patient,followed by fixation with a 10-0 nylon thread.In the bandage contact lens group,a piece of Bausch & Lomb pure vision chip was used to cover the patient's cornea.The best corrected visual acuity,surface asymmetry index (SAI),surface regularity index (SRI) and corneal astigmatism were recorded before and at 1 month and 3 months after operation.Wong-Baker face was used to score the pain intensity.Results Two weeks after operation,the fluorescein staining showed that positive staining presented in 7 eyes of the amniotic membrane group and in 1 eye of the bandage contact lens group,and the difference in the positive rate of both groups was statistically significant (P ＜ 0.05).There was no significant difference in the best corrected visual acuity between the two groups before and after the operation (all P ＞0.05).There was no significant difference in corneal astigmatism between the two groups before the surgery (P ＞ 0.05),but at 1 month and 3 months after operation,the bandage contact lens group was lower than the amniotic membrane group,and the differences were statistically significant (both P ＜ 0.05).The preoperative SRI and SAI of the amniotic membrane group were 1.52 ± 0.83 and 1.68 ± 0.62,respectively,as well as 1.61 ± 0.66 and 1.73 ± 0.59 in the bandage contact lens group,respectively,and there was no significant difference between the two groups (all P ＞ 0.05).At 1 month and 3 months after the treatment,the SRI (0.99 ±0.72,0.63 ±0.58) and SAI (0.61 ±0.53,0.54 ±0.28) in the bandage contact lens group were lower than those in the amniotic membrane group [(1.28 ± 0.67,0.85 ± 0.47) and (0.97 ± 0.47,0.82 ± 0.35)],and the differences were statistically significant (all P ＜ 0.05).On the day of the surgery and on the first day after surgery,the pain index of the bandage contact lens group was lower than that of the amuiotic membrane group,and the differences were statistically significant (all P ＜ 0.05).There was no significant difference in the pain index between the bandage contact lens group and the amniotic membrane group at 2 weeks postoperatively (P ＞ 0.05).Three months after operation,there was recurrence in one eye (1.64％) in the amniotic membrane group and two eyes (3.77％) in the bandage contact lens group,and the recurrence rate was not statistically different (P ＞ 0.05).Conclusion There are better improvment of corneal repair,lower corneal refraction and lower pain scores when the bandage contact lens is used in pterygium surgery when compared with amniotic membrane.