Objectives: To study the apoptosis induced by homocysteine (Hcy) and the protective effects of folic acid, VE and Se. Methods: Inverted microscopy and MTT test were used to observe the protection of these nutrients on the cytotoxic effect of Hcy. DNA agarose gel electrophoresis and flow cytometry were used to observe the inhibition of these nutrients on the apoptosis induced by Hcy. The level of MDA, the activity of SOD and GSH-Px and the generation of active oxygen in cells were also measured to investigate the mechanism of the protective effects. Results: Significant effects of folic acid, VE and Se on the protection of cell damages and the inhibition of apoptosis induced by Hcy were observed. These protective effects may be the results of decreasing MDA in cells provided by folic acid, VE and Se, increasing the activity of SOD by folic acid, increasing the activity of GSH-Px by folic acid and Se, and decreasing the generation of active oxygen by VE. Conclusions: The protective effects of folic acid, VE and Se on the damage of ECV304 cell from Hcy and the inhibitive effect on apoptosis induced by Hcy might be in different mechanisms.

0.05),and was superior in group C than that in groups A and B(P0.05),and was superior in group C than that in groups A and B(P

With the rapid development and wide application of forensic DNA technology, strict management and guidance are required. Conclusion could be gained the public and judge recognition, would be more scientiifc, accurate and convincing by using the standard operation. In recent years, forensic DNA standard technology has been rapid development, but there are still insuffcient. This paper will summarize the present situation and the existing problems of forensic DNA technology standards and prospects.

This paper reviews studies on the relationship between health insurance and health status both at home and abroad. First, we put forward three viewpoints on their relationship;Second, based on different data, we review the studies from three different perspectives; Third, we review the recent studies at home. The paper draws the conclusion as following:(1) most studies on observational data prove that there is relationship between health in-surance and health status. To establish the causal relationship between them, we must overcome the endogeneity of health insurance. ( 2 ) Different studies have different conclusions, which are caused by different methodology and subjects. (3)The conclusions cannot be generalized to whole populations. Future studies should focus on the effect of different health insurance on different populations.

Diet Therapy ; Feeding Behavior ; Humans ; Metabolic Diseases ; diet therapy

Child ; Child, Preschool ; Diarrhea ; virology ; Female ; Genotype ; Humans ; Infant ; Male ; Rotavirus ; classification ; genetics ; isolation & purification ; Seasons

Objective To explore the influence of psychological first aid training on cognitive appraisal of sudden events in medical students.Methods A medical university undergraduate students were selected and divided into the training group(n=42) and the control group(n=53).The former was treated with 20 hours short-term targeted psychological first aid,and the latter was treated as blank control.The SAM and LMSQ were adopted to measure students' cognitive appraisal before and after the training.Results After the training,the training group gained significant differences (P＜ 0.05) in threat (11.95 ±3.17) vs (10.45±2.48)(t=2.426,P=0.020),challenge (12.64t2.84) vs (14.40±1.95)(t=-3.304,P=0.002),controllable-by-self (13.05±3.15) vs (14.93±2.00) (t=-3.240,P=0.002),controllable-by-others (12.40±3.10) vs (14.52±2.52) (t=-3.553,P=0.001),stressfulness (13.26±2.69) vs (12.00±1.96) (t=2.592,P=0.013) of SAM,and Physical threat ((23.88±7.59) vs (19.76±4.82),t=3.057,P=0.004) of LMSQ compared with before training.Other factors had no significant difference(P＞0.05).Compared with two groups,the training group gained significant increase(P＜0.05) on the score differences for SAM factors (challenge (t=-2.466,P=0.016),controllable-by-self (t=-2.557,P=0.012),controllable-by-others (t=-2.800,P=0.006)) than the control group,and significant reduction (P＜0.05) in physical threat (t=-2.308,P=0.023) of LMSQ.Conclusion Short-term targeted psychological first aid training has certain effects on changing cognitive appraisal about sudden events for medical students.

Objective To improve the efficiency,repeatability,expansibility and maintainability and reduce the construction and maintenance cost of the PACS.Methods Struts and Hibernate frame works based on MVC and Object-Oriented Analysis(OOA) were used to build PACS.Results It saved more time for developers,and promoted the development efficiency.Conclusion It is rapid and efficient to use Struts and Hibernate frame in building PACS.[Chinese Medical Equipment Journal,2008,29(2):55-57]

Objective To establish a method for detection of RhD(+) red blood cells mixed in D-negative blood by flow cytometry(FCM).Method RhD(+) and RhD(-) RBCs were mixed according to predefined ratios.Cells were indirectly labeled,with IgG anti-D labeled as the first antibody,and FITC-anti-IgG F(ab')2 as the second antibody.The percentage of RhD(+) RBCs was determined by FCM,and the best dosage of IgG anti-D was also defined.The ratio of red cells in the two groups,measured by FCM,was compared with the actual ratio.The consistency of method was also evaluated.Results The effective dosage of IgG anti-D was 1∶4,and 50?l/1?106 cells.When the actual percentages of RhD(+) cell among RhD(-) cells were 2.5%-0.312%,the correlation coefficient between the percentages measured by FCM and the actual percentages was 0.987.The same tubes,containing 10% and 2.5% RhD(+) RBCs,were each tested for 10 times,and their coefficient of variation were 3.4%,and 4.9%,respectively.Conclusion The method of quantifying the RhD(+) RBCs in D-negative blood by FCM is feasible and repeatable,which deserves a further clinical application.

Objective To investigate the efficacy of microwave ablation for big liver cancer by using laparoscopy.MethodsFrom August 2000 to October 2006,28 patients with large big liver cancer(over 5 cm in diameter)was treated by microwave ablation under the guidance of a laparoscope.On each tumor nodule,3 to 5 electrode insertions was applied.And for each lesion,the treatment was performed for 1 to 3 times.Results The complete ablation rate of this series was 78.6%(22/28),and the rate of local recurrence was 35.7%(10/28).1-,3-,and 5-year survival rates were 75.0%(21/28),57.1%(12/21),and 36.4%(4/11),respectively.Conclusions Laparoscopic microwave ablation is safe and effective for liver cancer.The procedure can increased the efficacy of the microwave by enlarging the extent of the ablation.