Crystal HRP was applied to the cut ends of the left infraorbital nerve and branches of the facial nerve of 34 adult mice. Then the animals were killed and perfused, and HRP solution was applied to the cut ends of the root of the right facial nerve and the right maxillary nerve. Frozen sections of the brain stem and the trigerminal ganglia were made and reacted with BDHC, TMB or DAB method for light and electrom microscopic examination.Under light microscopy, the neurons labeled when alive in the left trigerminal ganglion and the nucleus of the left facial nerve showed that more cell bodise but less nerve fibers were labeled. The reaction products with all the 3 methods in thess cells were granular. The neurons labeled after death in the right trigerminal ganglion and the nucleus of the right facial nerve showed that more fibers but less cell bodies were labeled. The reaction products in these cells were also granular with BDHC and TMB methods but diffuse with DAB method.Under electron microscopy, there were electron-dense positive products in all the cells labeled either when alive or after death. In those cells labeled when alive, the products were found in the round or oval secondary lysosomes or multive-sicular bodies, while in those labeled after death, they were mostly found on one side of vacuoles without membrane boundary. In the fibers labeled after death, many large or small positive granules were found in the axons and many minute positive granules in the myelin lamellae especially on their inner surface in contact with the axons.

Objective To investigate the value of laparoscopic treatment for post-laparoscopic cholecystectomy complications.Methods From October 1996 to October 2007,totally 754 patients suffering from gallbladder diseases underwent laparoscopic cholecystectomy in our hospital;21 of them developed postoperative complications and received laparoscopic intra-abdominal exploration.Results By laparoscopy,bile leakage was diagnosed in 11 cases,intra-abdominal bleeding in 6,intraperitoneal fluid in 2,duodenal bulb perforation in 1,and obstructive jaundice in 1.The patients who developed obstructive jaundice was converted to open surgery because of hepatic duct transection.The other 20 cases underwent laparoscopic treatment.All the patients were followed up for a mean of 6 years(1 to 11),during which none of them had biliary complications.Conclusion Laparoscopic treatment is a microinvasive,safe and effective method for post-laparoscopic cholecystectomy complications.

Objective To explore the feasibility of applying laser scanning confocal microscopy to investigate the metastatic characteristic of tumor cells in vitro. Methods The confocal microscope combined with transwell assay was employed to observe the invasion and migration of the metastatic colon cancer ]ovo cells when stimulated by IL-8. Results The results show that the number of invasive lovo cells in the stimulated group was much higher than the unstimulated group (P<0.05). The migration way of lovo cells observed under the confocal microscopy was ameba like movement. These findings suggest that the metastatic ability of lovo cells stimulated by IL-8 was enhanced. Conclusion The metastatic characteristic of colon adenocarcinoma lovo cells is similar to the chemotactic movement of leukocytes.

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Chemotherapy, Adjuvant ; Genomics ; Humans ; Neoadjuvant Therapy ; Neoplasm Staging ; Proteomics ; Quality of Life ; Salvage Therapy ; Stomach Neoplasms ; drug therapy ; pathology ; surgery ; Survival Rate

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Chemotherapy, Adjuvant ; Colorectal Neoplasms ; radiotherapy ; surgery ; therapy ; Combined Modality Therapy ; Humans

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Chemotherapy, Adjuvant ; Chromosomal Instability ; Colonic Neoplasms ; drug therapy ; genetics ; pathology ; Deoxycytidine ; analogs & derivatives ; therapeutic use ; Fluorouracil ; administration & dosage ; analogs & derivatives ; therapeutic use ; Humans ; Leucovorin ; administration & dosage ; Microsatellite Instability ; Neoadjuvant Therapy ; Neoplasm Staging ; Organoplatinum Compounds ; administration & dosage ; Prognosis ; Rectal Neoplasms ; drug therapy ; genetics ; pathology ; Risk Factors

Antibodies, Monoclonal, Humanized ; therapeutic use ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Bevacizumab ; Gastrointestinal Neoplasms ; classification ; epidemiology ; therapy ; Humans ; Indoles ; therapeutic use ; Neuroendocrine Tumors ; classification ; epidemiology ; therapy ; Octreotide ; therapeutic use ; Pancreatic Neoplasms ; classification ; epidemiology ; therapy ; Peptides, Cyclic ; therapeutic use ; Pyrroles ; therapeutic use ; Sirolimus ; analogs & derivatives ; therapeutic use ; Somatostatin ; analogs & derivatives ; therapeutic use

Antibodies, Monoclonal, Humanized ; therapeutic use ; Antineoplastic Agents ; therapeutic use ; Chemoradiotherapy ; Chemotherapy, Adjuvant ; Esophageal Neoplasms ; classification ; pathology ; surgery ; therapy ; Esophagogastric Junction ; Humans ; Stomach Neoplasms ; classification ; pathology ; surgery ; therapy ; Trastuzumab

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Colonic Neoplasms ; drug therapy ; pathology ; Disease Progression ; Drug Administration Schedule ; Guidelines as Topic ; Humans ; Neoplasm Staging ; Survival Analysis

Communicable Diseases ; Disease Outbreaks ; Humans ; Infection Control ; Science