Crystal HRP was applied to the cut ends of the left infraorbital nerve and branches of the facial nerve of 34 adult mice. Then the animals were killed and perfused, and HRP solution was applied to the cut ends of the root of the right facial nerve and the right maxillary nerve. Frozen sections of the brain stem and the trigerminal ganglia were made and reacted with BDHC, TMB or DAB method for light and electrom microscopic examination.Under light microscopy, the neurons labeled when alive in the left trigerminal ganglion and the nucleus of the left facial nerve showed that more cell bodise but less nerve fibers were labeled. The reaction products with all the 3 methods in thess cells were granular. The neurons labeled after death in the right trigerminal ganglion and the nucleus of the right facial nerve showed that more fibers but less cell bodies were labeled. The reaction products in these cells were also granular with BDHC and TMB methods but diffuse with DAB method.Under electron microscopy, there were electron-dense positive products in all the cells labeled either when alive or after death. In those cells labeled when alive, the products were found in the round or oval secondary lysosomes or multive-sicular bodies, while in those labeled after death, they were mostly found on one side of vacuoles without membrane boundary. In the fibers labeled after death, many large or small positive granules were found in the axons and many minute positive granules in the myelin lamellae especially on their inner surface in contact with the axons.

Objective To investigate the value of laparoscopic treatment for post-laparoscopic cholecystectomy complications.Methods From October 1996 to October 2007,totally 754 patients suffering from gallbladder diseases underwent laparoscopic cholecystectomy in our hospital;21 of them developed postoperative complications and received laparoscopic intra-abdominal exploration.Results By laparoscopy,bile leakage was diagnosed in 11 cases,intra-abdominal bleeding in 6,intraperitoneal fluid in 2,duodenal bulb perforation in 1,and obstructive jaundice in 1.The patients who developed obstructive jaundice was converted to open surgery because of hepatic duct transection.The other 20 cases underwent laparoscopic treatment.All the patients were followed up for a mean of 6 years(1 to 11),during which none of them had biliary complications.Conclusion Laparoscopic treatment is a microinvasive,safe and effective method for post-laparoscopic cholecystectomy complications.

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Chemotherapy, Adjuvant ; Chromosomal Instability ; Colonic Neoplasms ; drug therapy ; genetics ; pathology ; Deoxycytidine ; analogs & derivatives ; therapeutic use ; Fluorouracil ; administration & dosage ; analogs & derivatives ; therapeutic use ; Humans ; Leucovorin ; administration & dosage ; Microsatellite Instability ; Neoadjuvant Therapy ; Neoplasm Staging ; Organoplatinum Compounds ; administration & dosage ; Prognosis ; Rectal Neoplasms ; drug therapy ; genetics ; pathology ; Risk Factors

Communicable Diseases ; Disease Outbreaks ; Humans ; Infection Control ; Science

Asthma ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Colonic Neoplasms ; drug therapy ; pathology ; Disease Progression ; Drug Administration Schedule ; Guidelines as Topic ; Humans ; Neoplasm Staging ; Survival Analysis

Objective To explore and summarize the clinical features and treatmentof the nonpuerperal mastitis.Methods Eighty-two cases of nonpuer-peral mastitis from 2003 to 2013 were analyzed retrospectively.All patients were divided into surgery alone group (11 cases)and medication combined surgery group (71 cases),which PDM patients with triple anti-mycobacterial drugs therapy,GM patients with cortical hormone therapy.Results All patients were diagnosed by biopsy.The total duration of simple drug group (triple or hormone) is (11.92 ±6.50) weeks and remission rate after 4 weeks was (89.06 ± 2.25) ％.Drugs plus surgery group,the total duration of the group is (6.57 ± 2.30) weeks,and no recurrence.The total duration of the simple surgery group is (17.09 ± 13.11) weeks and remission rate after 4 weeks was (63.64 ± 1.10) ％.Comparison of the total duration of the three groups and the cumulative response rate curves were significantly different(P ＜ 0.05).Conclusions Nonpuerperal mastitis requires a combination of clinical and pathological diagnosis.Surgery is not the only treatment option,triple anti-mycobacterial or cortisol hormones for different types of pathology conservative treatment can achieve a satisfactory therapeutic effect.Drug therapy combined with surgery can shorten the duration and reduce the relapse rate,it is an effective treatment for nonpuerperal mastitis.

Objective To explore the effect of the joint teaching rounds of the multidisciplinary radiotherapy.Methods 54 clinical college students of Grade 2013 was selected as the control group,adopting the traditional single teaching ward round model,while 58 clinical college students of Grade 2014 was selected as the research group,adopting the multidisciplinary joint radiation operation mode.By contrasting the satisfaction questionnaire,calculating and delineating the target volume error rate and the theory examination results,the effect of teaching ward round was evaluated.Results ①According to the survey,the research group's satisfaction in teaching methods,stimulating students' enthusiasm for learning,improving their clinical thinking ability and consolidating the knowledge (respectively 91.38％,86.21％,82.76％,93.10％),was higher than that of the control group (75.93％,68.52％,62.96％,77.78％),and the difference was statistically significant (chi square values were 4.95 5.05,6.25,5.36;P＜0.05).②The research group's pass rate in delineation of Gross Target Volume (GTV) and the Clinical Target Volume (CTV) was (82.76％,86.21％),higher than that of the control group (53.70％,62.96％),and the difference was statistically significant (chi square values were 14.36,9.48;P＜0.01).③Research group's score in clinical Three Basics and oncology theory achievement was(88.75 ± 5.23) and (82.72 ± 3.69),higher than the control group [(81.88 ± 613),(80.74 ± 3.25) points],and the difference was statistically significant (t=6.40 respectively,3.01;P＜ 0.05).Conclusion Radiotherapy department's multi-disciplinary joint ward round helps to improve the teaching quality.

Objective To investigate the influence of the different second stage of labor duration on the maternal and neo‐natal outcomes .Methods Data were retrospectively analyzed from 201 nulliparous women delivering a singleton infant in cephalic presentation without labor analgesia hospitalized in our hospital between January 2012 and May 2015 ,whose second stage pro‐gressed beyond 2 hours .They were divided into observation group and control group according to different second stage of labor du‐ration ,and cases with a second stage of labor duration of three hours were in the observation group (163 cases) ,and those within two hours were in the control group (38 cases) .Results Whether the limit of the second stage of labor duration of 2 hours ,or 3 hours ,the risk of postpartum hemorrhage ,postpartum fever ,urinary retention and neonatal asphyxia ,admission to the neonatology department in two groups were not significantly different(P>0 .05) .However ,there was significance difference in the rate of soft birth canal laceration(72 cases in the observation group) ,poor wound healing (0 case in the observation group and 3 cases in the control group ,P<0 .05) .Conclusion a second stage of labor duration of 3 hours could reduce the rate of cesarean section ,and with no increase in severe adverse maternal and neonatal outcomes .

98%. Conclusion The developed method is simple, reproducible and easy to operate. The solvent is cheap, with low boiling point and easy to recovery property.