OBJECTIVE:To analyze and identify the liposoluble constituents in Isatis indigotica.METHODS:The liposoluble constituents in Isatis indigotica were extracted with petroleum benzin,and the constituents in methyl ester products were sperated,identified and determined by GC/MS.RESULTS:A total of 42 liposoluble compounds were detected,with fatty acid methyl esters making up 36.72%(saturated fatty acid methyl esters 8.67% and unsaturated fatty acid methyl esters 28.05%),and of which,35 were reported for the first time in the study of Isatis indigotica.CONCLUSION:The liposoluble compounds in Isatis indigotica serve as reference for its comprehensive benefits and development of its antioxydant applilication.

Objective:To assess the efficacy and tolerability of sibutramine as a weight reduction agent in treatment of overweight patients with type 2 diebetes.Methods: Forty overweight patients(body mass index ≥25 kg/m~(2)) with type 2 diebetes were randomized into sibutramine treatment group and control group(n=20).Both groups received a reduced-calorie diet(deficit of 1 045-2 090 kJ/d from an average daily intake) and continued their original treatment;sibutramine treatment group was also given sibutramine 15 mg once daily for 12 weeks.Before and after sibutramine treatment,body mass index(BMI),fasting blood glucose(FBG),fasting serum insulin(FINS),hemoglobin A_(1C)(HbA_(1C)),and serum lipids were measured in the patients.Homeostasis model assessment(HOMA) was applied to assess the status of insulin resistance(IR).Results: After treatment,BMI,FBG,HbA_(1C),_()TG,FINS and HOMA-IR all decreased(P

For the purpose of creating a hospital culture, the present paper summarizes theories on research-oriented organizations, addresses the significance of developing a research-oriented hospital, summarizes the means and methods for developing a research-oriented hospital according to the model of a research-oriented organization, and demonstrates the effects of a research-oriented hospital and promoting functions for hospital culture.

Central post-stroke pain refers to pain resulting from a primary lesion or dysfunction of the central nervous system after a stroke.The prevalence of central post-stroke pain varies from 8％ to 46％.This article reviews the definition,epidemiology,pathophysiology,clinical features,and treatment of central post-stroke pain.

Severe acute respiratory syndrome(SARS)is caused by a new type of coronaviruses.The structure,pathogenicity,resistance of SARS-CoV and the current status and shortage of laboratory diagnosis for SARS was reviewed in this literature.

Objective　To observe the effect of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line. Methods　The effect of drugs on the cells was measured by MTT, and the protein expression of cyclin D1 gene in the cells was measured by immunohistochemistry assay. Results　①The anti-tumor effects of drugs on the cells were increased with the increasing of concentrations, and had a combined function between NDGA and MTX or VCR. ②There was no difference between the effect of treating NDGA with MTX or VCR given at the same time and MTX or VCR given after 24 h of NDGA treatment, but the effects were apparently different when NDGA was administrated after MTX or VCR treatment for 24 h. ③The protein expression of cyclin D1 gene in the cells was apparently reduced after NDGA treatment. Conclusion　There is a cooperative function between NDGA and MTX or VCR on the cells, and the function is probably related to the NDGA-induced down-regulation of protein expression of cyclin D1 gene in the cells.

Objective To discuss the CT features of mediastinal teratoma.Methods 35 patients with mediastinal teratoma confirmed by clinical and pathologic data were retrospectively analyzed.Results Most mediastinal teratomas(97.1%) were localized in the middle or upper area of anterior mediastinum.Mediastinal teratoma included cystic teratoma(60.0%) and solid teratoma(40.0%).All kinds of tissues could be found in the tumors.CT features of cystic teratoma(n=21) were the cystic mass with fluid density,most of them(n=19) had the characteristics of "obviouswalled cysts".CT features of solid teratoma(n=14) were the soft-tissue mass with mixed densities.CT features of malignant teratoma(n=5) were that the layer of fatty tissue between the tumor and the surrounding structures disappeared,and that the wall of neighbour vessels were mostly or all surrounded by the tumor.Mediastinal teratoma could cause pleural offusion(n=6) and pericardial offusion(n=12).Conclusion Mediastinal teratomas have obvious CT imaging features.CT has very important value in the diagnosis and differential diagnosis of mediastinal teratoma.

Purpose To evaluate the correlation between acute pulmonary embolism (APE) and right ventricular parameters by multi-slice spiral computed tomography pulmonary angiogmphy (MSCTPA),and assess the severity and mortality of patients with APE.Materials and Methods The clinical and MSCTPA image data of APE patients from January 2012 to November 2015 was analyzed retrospectively.All the patients were divided into three groups according to the pulmonary embolism type and clinical prognosis:66 cases in mixed embolism group (25 cases of death,41 cases of survival);36 cases in peripheral embolism group,2 cases in centralembolism group.The CT post-processing software was used to measure thoracic aorta and main pulmonary artery inner diameter ratio (PA/AO),right ventricular diameter to left ventricular diameter ratio (RVD/LVD);right ventricular area to left ventricular area ratio (RVA/LVA) on axial four-chamber (4-CH) sections and the largest of maximum minor axis diameters on axial sections (RV/LV-LD).The difference of CT related right ventricular parameters among the mixed embolism groups,peripheral embolism and normal groups was compared.Results Statistical difference was found in right ventricular parameters (RVD/LVD,RV-LD/LV-LD,RVA/LVA) in mixed embolism death patients compared with the patients in other three groups (P＜0.05).There was significant difference of right ventricular parameters RVD/LVD,RVA/LVA in mixed pulmonary embolism survival patients compared with patients in peripheral pulmonary embolism and normal groups (P＜0.05),but there was no significant difference between peripheral APE patients and the control group in all RV and PA/AO parameters (P＞0.05).According to the result of ROC curve in mixed type embolism death patients,RVA/LVA was the best indicator of predicting early death (AUC=0.881),with 100.0％ sensitivity and 73.2％ specificity.Conclusion The right ventricular parameters by multislice CT pulmonary angiography can assess APE patient's condition and forecast their early mortality;and the clinical diagnosis accuracy of RVA/LVA is higher.

With the development of ultrasonic imaging technique,the thyroid nodule detection rate is ever increasing.However,there is still no unique characteristic for the identification of benign and malignant lesions in ultrasonogram.On the basis of conventional ultrasound performance of nodules,thyroid imaging reporting and data system for the correct classification of thyroid nodules is conducive to their differential diagnosis.Contrast enhanced ultrasound (CEUS) can show the microvascular status of the lesion,and the benign and malignant thyroid nodules can be analyzed by observing the different enhancement patterns.By evaluating the difference in hardness between different tissues,the ultrasonic elastography (.UE) provides a complementary method for the identification of benign and malignant thyroid nodules.Ultrasound-guided fine-needle aspiration biopsy (US-FNAB) can accurately locate and offer pathological basis for preoperative diagnosis of thyroid nodules.It is helpful to preoperatively diagnose the thyroid cancer by detecting the molecular marker of the biopsy specimens which are difficult to be diagnosed clearly.

Objective To investigate the clinical effect of voriconazole in the treatment of invasive fungal infections in lung cancer patients with radiotherapy and chemotherapy,to provide a reference for clinical treatment. Methods The clinical data of 64 lung cancer patients with radiotherapy and chemotherapy with invasive fungal infec-tions were retrospectively analyzed.According to the treatment methods,they were divided into control group and observation group,32 cases in each group.Fluconazole treatment was used in the control group,and the observation group used voriconazole therapy.The overall response rate,time to return to normal white blood cells and other indica-tors of liver and kidney function were compared between the two groups.Results The effective rate of the observation group was 81.25%,which was significantly higher than 68.75% of the control group,the difference was statistically significant(χ2 =5.876,P=0.008).The temperature recovery,cough disappeared,leukocyte recovery time of the observation group were (3.61 ±0.61)d,(6.11 ±0.81)d,(7.85 ±0.92)d,which were significantly shorter than those of the control group,the differences were statistically significant(t=4.145,P=0.025;t=4.045,P=0.027;t=4.385,P=0.021).The treatment of the two groups had mild liver and kidney damage,and there was no signifi-cant difference between the two groups(all P>0.05 ).Conclusion Voriconazole treatment has good clinical effect for lung cancer patients after radiotherapy and chemotherapy with invasive fungal infections,the treatment period is short,but with mild liver and kidney function impairment,the treatment should strengthen the monitoring.