Aged ; Humans ; Oligonucleotide Array Sequence Analysis ; Presbycusis ; genetics

The association between psychogenic illness and human endogenous viruses (HEVs), including human endogenous retrovirus and Borna disease virus, remains unclear. As the component of human genome, HEVs may become the joint of various pathogenic factors of schizophrenia (SZ), such as heredity, environment, and immunity. In this review, we strive to uncover the clinical and laboratory evidence for the roles and possible pathogenic mechanism of HEVs in the development of SZ.
Animals ; Environment ; Humans ; Schizophrenia ; etiology ; genetics ; immunology ; virology ; Viruses ; genetics

The determination and characterization of solid drug form polymorphisms plays an important role in drug quality control, selection of the production process and clinical efficacy evaluation. Vibrational spectroscopy is a powerful method for the characterization of drug polymorphisms. In this paper we review recent research and application advances in the polymorphic characterization of active pharmaceutical ingredients (APIs) and drug cocrystals/salts by using Fourier transform infrared (FTIR) and Raman spectroscopy to elucidate the characteristics of APIs and drug complexes. This may provide theoretical support for structural analysis during the development process for drugs.

Objective: To investigate the effect of PKG II (cGMP-dependent protein kinase II) on cell proliferation-related MAPK (mitogen-activated protein kinase)/ERK (extracellular signal-regulated kinase) downstream targets RSK1 (ribosomal S6 kinase 1) and proto-oncogenes c -Jun and c -Fos in gastric cancer SGC-7901 cells. Methods: Gastric cancer SGC-7901 cells were infected with Ad-LacZ and Ad- PKG II adenovirus, and then the PKG II was overexpressed in SGC-7901 cells. These cells were treated with PKG II specific activator - 8-pCPT-cGMP [8-(p-chlorophenylthio)-cyclic GMP], and then they were stimulated with EGF (epidermal growth factor). The proliferation of SGC-7901 cells was examined by MTT method, and the mRNAs expression levels of c-Jun and c-Fos and the protein expression levels of p-RSK1, c-Jun and c-Fos were examined by RT-PCR and Western blotting, respectively. The nuclear accumulation of p-RSK1 was observed under an immunofluorescence microscope. Results: EGF-induced increase of cell proliferation and the elevation of expressions of c-Jun and c-Fos mRNAs and proteins as well as p-RSK1 (Ser380-) protein in SGC-7901 cells which were infected with PKG II were significantly inhibited after treatment with 8-pCPT-cGMP. The phosphorylation of RSK1 (Ser380) was decreased, and the accumulation of p-RSK1 (Ser380) in nuclei of SGC-7901 cells was increased after stimulation with EGF, while it was decreased after treatment with 8-pCPT-cGMP. Conclusion: Activated PKG II can inhibits the proliferation of gastric cancer cells, phosphorylation of RSK1 (Ser380), nuclear accumulation of p-RSK1(Ser380) and the expressions of c -Jun and c -Fos genes which were induced by EGF. Copyright © 2012 by TUMOR.

Humans ; Male ; Middle Aged ; Pharynx ; pathology ; Pyriform Sinus ; Thyroglossal Cyst ; surgery

Acute Disease ; Case-Control Studies ; Child ; Child, Preschool ; Female ; GATA3 Transcription Factor ; genetics ; Humans ; Infant ; Interferon-gamma ; genetics ; Interleukin-4 ; genetics ; Male ; Mucocutaneous Lymph Node Syndrome ; blood ; genetics ; immunology ; RNA, Messenger ; Reverse Transcriptase Polymerase Chain Reaction ; T-Box Domain Proteins ; genetics ; Th1 Cells ; immunology ; Th2 Cells ; immunology

Aneuploidy ; Animals ; Cervical Intraepithelial Neoplasia ; genetics ; pathology ; DNA, Neoplasm ; analysis ; Diagnosis, Differential ; Female ; Image Cytometry ; methods ; Neoplasms, Squamous Cell ; genetics ; pathology ; Prognosis ; Uterine Cervical Neoplasms ; genetics ; pathology

Bioreactors ; Humans ; Liver Failure, Acute ; therapy ; Liver, Artificial ; classification ; Sorption Detoxification ; instrumentation ; methods

Autoimmune Diseases ; diagnosis ; immunology ; pathology ; therapy ; Child ; China ; Gastroenterology ; Hepatitis B ; diagnosis ; pathology ; therapy ; Humans ; Infant

Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Immunoglobulin D ; Male ; Middle Aged ; Multiple Myeloma ; classification ; pathology ; Retrospective Studies