Air ; analysis ; Chromatography ; methods ; Ions ; analysis ; Metals, Alkali ; analysis ; Workplace

Hydrogen Peroxide ; analysis ; Sensitivity and Specificity ; Spectrophotometry ; methods ; Titanium ; Workplace

Objective To construct luciferase reporter plasmid containing synthetic CArG elements and to investigate their radiation-inducible property in tumor cells. Methods Insert chimeric regulation elements consisting of nine tandem-repeat copies of CArG sequence (CCATATAAGG) and CMV IE basal gene promoters into pGL3-Basic vectors to construct luciferase reporter plasmids. Tumor cells(HeLa, A549 and HepG2) were transiently transfected by reporter plasmids using lipofectamine, and transfected cells were irradiated by ?-ray with different doses. After 36 h, we assayed the level of reporter gene expression. Results The CArG elements could successfully induce the expression of a downstream reporter gene following irradiation, with maximal expression seen after 3 Gy irradiation. Conclusion The synthetic CArG elements are responsive to low dose of radiation and are able to enhance down-stream gene expression. It is expected to be the essential potential for future application of radiogenetic cancer therapy.

BACKGROUND: Microcirculation disturbance of pharynx may be one of the pathogenesis base caused recurrent attacks of chronic pharyngitis. The negative pressure can improve the microcirculation of inflammary target organs by biomechanical changes.OBJECTIVE: To explore the therapeutic effect of oral negative pressure on patients with chronic pharyngitis and nail fold microcirculation.DESIGN: A randomized single blind controlled observation.SETTING: Department of Pathophysiology of Hebei North University.PARTICIPANTS: A total of 65 patients with chronic pharyngitis and course of disease about 1-3 years admitted to Department of Neurology,First affiliated Hospital of Hebei North University from January to September 2002 were selected. They were randomly divided into treatment group (n=35) and control group (n=30), including 36 cases with chronic pharyngitis (18 cases from the treatment group, 18 cases from the control group), 29with chronic hypertrophied pharyngitis (17 cases from the treatment group,12 cases from the control group).METHODS: The Hices oral negative pressure instrument was used and the negative pressure exert equipment was put into the optimal position of the patients' mouth to keep the negative pressure of (0.05±0.01) Mpa, 10minutes once (the first therapy time was 5 minutes), once daily and the time was fixed, 5 days as a period of therapy for three periods. Patients in the control group were also given the negative pressure exerts equipment,but no negative pressure was exerted.MAIN OUTCOME MEASURES: ① Observation and evaluation of nail fold microcirculation: The nail fold microcirculation was observed by the XTL- Ⅱ microcirculation micro-television system, and the first row nail fold of the left ring figure was checked conventionally to record the state of microvessel, state of micro-bloodstream and peri-loop. The degree of microcirculatory disturbance was quantitatively analyzed according to Tian Niu's weighing integral method, the lower the scores, the better the microcirculation. ②The improvement of chronic pharyngitis after treatment of 3courses.RESULTS: Totally 65 patients with chronic pharyngitis entered the stage of result analysis. ①After 1 course of treatment, the symptoms of 1/3 of the patients in the treatment group were significantly improved; after 3 courses of treatment, except there was no obvious changes of hypertrophied morphology such as lymphatic follicle proliferation on posterior wall of throat and hypertrophy on side cable of throat, the pathological signs and prevalence of mucosa congestion were obviously lower than those before treatment and the control group (P ＜ 0.05-0.01) effective rate was 88.6%.②) Improvement of microcirculation disturbance with chronic pharyngitis was evident, restoring to basically normal from medium degree abnormality before treatment, the total score values reduced to 1.941 ±0.165 from 4.836±0.242 (P ＜ 0.01).CONCLUSION: Oral negative pressure can significantly release or eliminate the symptoms and signs of chronic pharyngitis, which may relate to improvement of microcirculation disturbance.

Objective To study the activity of the survivin gene promoter in several tumor cell lines and evaluate the possible application of this promoter in tumor gene therapy. Methods ①The expressions of survivin gene in A549,MDA-MB231 and HepG2 cell lines were detected by RT-PCR and Western blotting.②Tumor cells(A549,MDA-MB231,HepG2) were transiently transfected by reporter plasmids containing different length of survivin promoter using lipofectamine.And 48 h later,the level of reporter gene expression was analyzed.Results There were different levels of survivin expression in A549,MDA-MB231 and HepG2 cell lines.Transient transfection assay approved that pLuc-surP-987,pLuc-surP-596,pLuc-surP-269 and pLuc-surP-158 showed high activity and 269 bp survivin promoter demonstrated the highest activity. Conclusion In transcriptional level,survivin promoter can activate the reporter gene in several tumor cell lines.It is a potential candidate promoter in tumor gene therapy.

OBJECTIVETo study the diagnosis and treatment of varices of the vocal cord.

METHODSThe clinical data of 21 cases with varix of vocal cord were analyzed. All the patients presented hoarseness. There were 15 female and 6 male cases with their ages ranged from 23 to 68 years (median 44 years old). The varix was found on the right vocal cord in 12 cases, on the left vocal cord in 9 cases. Isolated varix existed on the vocal cord in 10 cases, varix with vocal cord polyps or nodules in 10 cases, varix with vocal cord paralysis in 1 case.

RESULTSAll the patients were diagnosed under the laryngovideoscopy. The lesions appeared on the superior surface of the vocal cord. Varices manifested as abnormally dilated capillary running in the anterior to posterior direction in 6 cases, as clusters of capillary in 3 cases, as a dot or small sheet or short line of capillary in 12 cases. The varices were disappeared in 2 of 8 cases with vocal cord varices and polyps after removed the polyps. The varices of others patients had no change after following up for more than 6 months, but one patient happened hemorrhage of the contralateral vocal cord.

CONCLUSIONSVarices are most commonly seen in female. Laryngovideoscopy is the key in determining the vocal fold varices. Management of patients with a varix includes medical therapy, speech therapy, and occasionally surgical vaporization.

Adult ; Aged ; Female ; Humans ; Laryngoscopy ; Male ; Middle Aged ; Varicose Veins ; diagnosis ; therapy ; Vocal Cords ; blood supply ; pathology ; Young Adult

Objective To explore the relationship between obstructive sleep apnea-hypopnea syndrome (OSAHS) and hypertension.Methods There were 1149 middle aged patients who were suspected to have OSAHS underwent polysomnography (PSG) from Jan,1999 to June,2011.The age of the patients ranged from 45 to 65 years. The patients were divided into 10 groups according to sleep apnea hypopnea index (AHI),group 1:AHI ＜5; group 2:5≤AHI ＜ 15; group 3:15≤AHI ＜30; group 4:30≤AHI ＜40 ; group 5:40 ≤ AHI ＜ 50 ; group 6:50 ≤ AHI ＜ 60 ; group 7:60 ≤ AHI ＜ 70 ; group 8:70 ≤ AHI ＜ 80 ;group 9:80 ≤ AHI ＜ 90; group 10:AHI ≥90.The incidence of hypertension in each group was tested using crosstabs method.The correlation between AHI,lowest oxygen saturation (LSaO2 ),age,body mass index (BMI) and hypertension were analyzed.Results Among 1149 suspected OSAHS patients,1004 were diagnosed as OSAHS by PSG.There were positive correlation between AHI and diastolic,systolic blood pressure ( r were 0.239 and 0.305,respectively,P ＜ 0.01 ). AHI was independent risk factor of hypertension(P ＜0.001 ).No statistically significant difference in the incidence of hypertension was found among group 1 and group 2,3,group 4 and group 5,group 6 to group 10 ( x2 were 3.821,0.005 and 1.325,P ＞ 0.05,respectively).There was significant difference in the incidence of hypertension among group 4 and group 1,2,3,group 5 and group 6 ( x2 were 2.615 and 4.300,P ＜0.05,respectively).There were two cutoff points at the AHI of 30 and 50.Conclusion With OSAHS exacerbations,the incidence of hypertension was significantly increased.

OBJECTIVETo observe the clinical shapes of vocal cord polyps and their pathological features.

METHODSThe laryngoscopic photographs of 156 patients with vocal cord polyps were reviewed. All patients received phonomicrosurgery. Eighty eight males and 68 females were included. Their ages ranged from 14 to 76 years (median 43.0 years). The clinical courses of the diseases were 1 to 132 months (median 6.5 months). There were 57 cases with right vocal cord polyps, 61 cases with left vocal cord polyps, 38 cases with bilateral vocal cord polyps. The vocal cord polyps with pedicles were found in 27 cases, without pedicles in 129 cases. The specimens of the vocal cord polyps were fixed in formalin solution and embedded in paraffin. Sections were cut, stained with HE method, and observed under light microscope.

RESULTSThe vocal cord polyps were classified into 5 types according to the clinical shapes of vocal cord polyps and their pathological features: edematous type in 70 cases (44.9%), vascular type in 49 cases (31.4%), fibrous type in 15 cases (9.6%), hemorrhagic or thrombotic type in 13 cases (8.3%), and amyloid type in 9 cases (5.8%). The clinical courses among the five types of vocal cord polyps had no statistic significance (P > 0.05).

CONCLUSIONSThe classification of the vocal cord polyp according to the clinical shapes of vocal cord polyps has clinical significance for the treatment. Some vocal cord polyps of edematous type, vascular type and hemorrhagic type can be cured with voice therapy. The vocal cord polyps of fibrous type and amyloid type need treatment with phonomicrosurgery. The differentiation diagnosis between amyloid type vocal cord polyps and vocal cord carcinoma should be made.

Adolescent ; Adult ; Aged ; Female ; Humans ; Laryngeal Neoplasms ; pathology ; Laryngoscopy ; Male ; Middle Aged ; Polyps ; pathology ; Vocal Cords ; pathology ; Young Adult

Objective To evaluate the value of electronic laryngoscope for transnasalesophagoscopy.Methods The electronic laryngoscope was used for transnasal esophagoscopy in 50 patientsfrom June 2009 to June 2011.There were 32 males and 18 females with their age ranged from 16 -88 years (mean 53.8 years).Before esophagoscopy,1％ ephedrine-dicaine mixture was sprayed into the nasal and pharyngeal cavities for topical anesthesia.The esophagoscopy was used for screening examination in the patients with reflux,globus sensation,dysphagia,head and neck cancer,suspected foreign body,and vocal cord paralysis,etc.Results The transnasal esophagoscopy with electronic laryngoscope was performed successfully in all the patients.Mild nausea and vomiting occurred in 4 patients,but no patient required to stop the examination.Of the 50 patients,38 patients (76％) had normal findings and 12 patients (24％)had positive findings of the esophagus.Esophageal cancer was diagnosed in one patient,esophageal foreign body in 2 patients,esophageal injury in one patient,candidal esophagitis in 3 patients,esophagitis in 3 patients,and achalasia of the cardia in 2 patients.Conclusions The electronic laryngoscope for transnasal esophagoscopy is an alternative to conventional esophagoscopy,which is useful for screening the esophagus in the patients with reflux,globus,dysphagia,head and neck cancer,and suspected foreign body,etc.

OBJECTIVESTo explore the relationships between the peripheral blood levels of CD61, CD63, PAC-1 and the incidence of acute rejection and tubular necrosis after renal transplantation, and recovery of the graft function.

METHODSThe peripheral blood levels of CD61, CD63, and PAC-1 of 86 patients with uremia in different stages before and after transplantations were analyzed by flow cytometry. The patients were divided into three groups: (1) twenty-nine patients with normal grafts function, (2) hirty with acute rejection and (3) twenty-seven with acute tubular necrosis. The patients with acute rejection were randomly divided into treatment group with anticoagulants and cntrol group.

RESULTSThe peripheral blood levels of CD61, CD63 and PAC-1 significantly increased (P < 0.05) in the patients with acute rejection, in comparison with those with normal grafts function and those with acute tubular necrosis. The peripheral blood levels of CD61, CD63 and PAC-1 in patients with acute rejection in anticoagulants therapy was lower, recovery time of the grafts function was shorter, one-year survival rates of patients and grafts were higher, as compared with those of controls.

CONCLUSIONSThe patients with acute rejection have significantly high peripheral blood levels of CD61, CD63 and PAC-1 before transplantation, however, these values in patients with acute tubular necrosis are not high, this suggesting that acute rejection might relate to platelet activation, while acute tubular necrosis might not relate to it. After anticoagulants therapy in patients with acute rejection, the grafts function might recover faster and their one-year survival rates and grafts might be higher in those with CD61, CD63 and PAC-1 decreasing remarkably.

Adult ; Aged ; Antigens, CD ; blood ; Dual Specificity Phosphatase 2 ; Female ; Graft Rejection ; Humans ; Integrin beta3 ; blood ; Kidney ; physiopathology ; Kidney Transplantation ; Male ; Middle Aged ; Platelet Activation ; Platelet Membrane Glycoproteins ; Protein Phosphatase 2 ; Protein Tyrosine Phosphatases ; blood ; Tetraspanin 30