AIM: To establish a RP HPLC method to determine the content of alliin in Allium sativum Linn. METHODS: Chromatographic separation was performed on ODS column with a mobile phase composed of methanol water (3∶7 by volume). The eluate was monitored at 214nm wavelength. RESULTS: The average recovery of sample for alliin was 98.7%, The RSD of within day was 1.1%. The RSD of between day was 1.9%. CONCLUSION: The method is simple, rapid with good suitability.

Objective To explore the effects of peripheral stem cell transplantation on extremely severe bone marrow form of acute radiation sickness.Methods One patient was radiated aecidently with the radiation dosage of 9～15 Gy and diagnosed as extremely severe bone marrow form of acute radiation sickness.Pretreatment was performed at 4th day after the accident and 3 days later,HLA- matched allogeneic stem cell transplantation on the patient was performed.Graft versus host disease (GVHD) was prevented with cyclosporin A (CsA) and mycophenolate mofetil (MMF).Results The haematopoiesis was recovered at 9th day after transplantation.At 11th day after transplantation, WBCs were increased to 14.74?10~9/L and returned to the normal levels subsequently,number of platelets risen to 51?10~9/L and hemaglobin was over 80 g/L.TRS-PCR and blood type dynamic detection testified that the donor's cells were stably implanted,the chromosome aberration and micro- nuclei disappeared after transplantation,and the patient's blood type changed into the donor's at 27th day after transplantation.But the radiation injury were still getting worse complicated with multiple infections,At 68th day after transplantation (75 days after the accident),the patient was died of mul- tiple organs failure.Conclusion Extremely severe bone marrow form of ARS can achieve hematopoie- sis recovery by allogeneic stem cell transplantation,but only hematopoiesis recovery can not cure the immunodefficiency and the radiation injury of the whole body.

It is a trend that innovate the traditional bilingual education model and select a new teaching model.Code-switching is a lingual phenomenon when a passages or articles are expressed with two or more language.To guarantee effect of bilingual education and improve education quality,penetration bilingual education was applied during microbiology teaching.Professional English vocabulary,words,passages or articles were introduced to students timely and by measure by the way of language code-switching.The results showed that bilingual teaching mode with language code-switching inspire study emotion and self-confidence of English expression from students.

To study the preventive effect of sophocarpine (Soc) on dextran sulfate sodium (DSS)-induced colitis in mice, in order to analyze the influence of Soc on toll like receptor 4 (TLR4)/mitogen-activated protein kinases (MAPKs) and janus tyrosine kinase 2 signal transducer and activator of transcription 3 (JAK2/STAT3) signal pathways in mice intestinal tissues. The mice was given 2.5% DSS for 6 days to induce the acute colitis model. The Soc-treated group was intraperitoneally injected with sophocarpine 30 mg · kg(-1) · d(-1) since the day before the experiment to the end. The disease activity index (DAI) was assessed everyday, and the colonic morphology and histological damage were observed with HE staining. The mRNA expressions of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were detected by real-time RT-PCR. The changes in key protein kinase p38 mitogen-activated protein kinase (p38MAPK), c-Jun NH2-terminal protein kinase1/2 (JNK1/2), extracellular signal-regulated kinase1/2 (ERK1/2), JAK2, STAT3 in TLR4/MAPKs and JAK2/STAT3 signaling pathways were detected by western blot. The result showed that the model group showed statistical significance in body weight, DAI, colon length and histopathological changes compared with the normal group (P <0.05); however, the Soc-treated group showed significant improvements in the above indexes compared with the model group (P <0.05). TNF-α, IL-1β and IL-6 in the model group was significantly higher than that in the normal group (P <0.05), but lowered in the Soc-treated group to varying degrees (P <0.05). In the normal group, the expressions of TLR4 and the phosphorylation of P38, JNK1/2, JAK2, STAT3 were at low levels; in the model group, the phosphorylation of P38, JNK1/2, JAK2, STAT3 increased; the Soc-treated group showed a decrease in TLR4 expression compared with the model group, with notable declines in the phosphorylation of TLR4, P38, JNK1/2, JAK2, STAT3. These findings indicate that Soc can inhibit TLR4/MAPKs, K2/STAT3 signaling pathway activation, reduce the expression of proinflammatory cytokines TNF-α, IL-1β and IL-6 and relieve inflammatory reactions, so as to effectively prevent experimental colitis.
Alkaloids ; pharmacology ; therapeutic use ; Animals ; Colitis ; drug therapy ; immunology ; pathology ; Cytokines ; genetics ; Janus Kinase 2 ; antagonists & inhibitors ; physiology ; Male ; Mice ; Mice, Inbred BALB C ; Phosphorylation ; STAT3 Transcription Factor ; antagonists & inhibitors ; physiology ; Toll-Like Receptor 4 ; antagonists & inhibitors ; physiology

Abducens Nerve Diseases ; therapy ; Acupuncture Points ; Adult ; Aged ; Electroacupuncture ; Female ; Humans ; Male ; Middle Aged ; Paralysis ; therapy ; Treatment Outcome ; Young Adult

Acupuncture Points ; Adolescent ; Adult ; Aged ; Coma ; drug therapy ; etiology ; therapy ; Combined Modality Therapy ; Craniocerebral Trauma ; complications ; Drugs, Chinese Herbal ; administration & dosage ; Electroacupuncture ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Objective To investigate the expression and effect of secondary lymphoid tissue chemokine(SLC)in experimental ulcerative colitis(UC)in rats.Methods Thirty Spague-Dawley rats were divided into control group and UC group.SLC expression in colon tissues was detected by RT-PCR and immunohistochemistry,respectively.Results The transcriptional level of SLC in UC tissues was significantly higher than that in the control group(0.846?0.07 vs 0.312?0.12,P＜0.01).The positive expression of SLC was concentrated mainly on submucosa,and the positive rate of SLC protein expression in UC group significantly higher than that in control group(P＜0.01).Conclusion SLC overexpression could contribute to the pathological processes in UC rats,thus SLC may be an ideal ther- apeutic target for UC.

Objective To study the consumption rate of qualified iodized salt at household level based on the salt surveillance results from 2004 to 2011,and to provide a scientific basis for setting up appropriate control strategies to iodine deficiency disorders.Methods Iodized salt monitoring results in Hainan Province from 2004 to 2011 were collected with retrospective method.Coverage rate of iodized salt,qualified rate of iodized salt and consumption rate of qualified iodized salt were calculated at the provincial,city(county) levels and on geographic distribution (coastal,plains and mountains).Qualified iodized salt criteria was set as (35 ± 15)mg/kg,unqualified iodized salt criteria was set as 5 to ＜ 20 mg/kg or ＞ 50 mg/kg,and criteria of non-iodized salt was set as ＜ 5 mg/kg.Results From 2004 to 2011,at provincial level,the median of iodized salt was raised from 30.25 mg/kg to 32.14 mg/kg; the iodized salt coverage rate,the qualified rate of iodized salt and the consumption rate of qualified iodized salt was raised from 77.81％ (4780/6143) to 96.06％ (5890/6132),90.48％ (4325/4780) to 98.72％(5815/5890),and 70.41％(4325/6143) to 94.83％(5815/6132),respectively.From 2004 to 2011,at city (county) level,the proportion of iodized salt coverage rate that higher than 90.00％,of qualified rate of iodized salt that higher than 95.00％ and of consumption rate of qualified iodized salt that higher than 90.00％ was raised from 44.45％(8/18) to 88.89％(16/18),16.67％(3/18) to 100.00％(18/18),and 22.22％(4/19) to 88.89％(16/18),respectively.The iodized salt coverage rate in the coastal and plain townships was raised from 70.55％(1440/2041) to 95.02％(1869/1967),and 75.36％ (1762/2338) to 96.24％(2331/2422),respectively.The iodized salt coverage rate in mountainous townships maintained at 89.46％ (1578/1764)-97.46％ (1690/1734) in the 8 years.There were 2 counties where the iodized salt coverage rate was less than 90％.There were 9 and 4 townships,where the iodized salt coverage rate was less than 90％ in coastal and plain townships,respectively,in 2011.Conclusions The rate of qualified iodized salt has been raised in Hainan Province,but part of coastal and plain townships (towns) are still serious in non-iodized salt problem.Comprehensive intervention on iodine deficiency disorders should be strengthened in these areas.

Objective:To discuss the topical action characteristics of the biological transmission of moxibustion heat via temperature collection and numerical modeling.Methods:Temperature of moxibustion was measured at multiple points at a distance of 3 cm to obtain the moxibustion temperature field nephograms by the high-accuracy temperature measure array.Finite element analysis was used to imitate the three-dimensional dynamic distribution of temperature in acupoint tissues.Results:Through numerical analysis,the one-dimensional,two-dimensional and three-dimensional distributions of temperature in human acupoint tissues at 5 min of moxibustion were established.The result showed that moxibustion heat mainly transmitted from the surface of the tissue to the internal,and the influence of moxibustion heat decreased with the depth of the tissue.The analysis of the nephograms of acupoint tissue temperature at 5,10,15 and 20 min of moxibustion showed that with the increase of the moxibustion time,the temperature in acupoint tissues constantly rose,and the transmission depth of moxibustion heat also further expanded inside acupoint.Conclusion:By establishing the three-dimensional dynamic model of heat transmission inside acupoint tissues with the biological parameters of human tissues and the temperature values obtained,this study used finite element analysis software ANSYS 14.0 and discovered the rules in the transmission of heat in body tissues during moxibustion,and the features in moxibustion heat transmission (from the proximal to the distant) and heat penetration (from the surface to the internal).This study provides theoretical and experimental support for the application of moxibustion in clinical practice.

Silica gel column chromatography was used for the isolation and purification of the chemical constituents of the pericarp of Illicium macranthum. From dichloromethane-EtOAc (1:1) fraction and EtOAc fraction of the methanol extracts, eleven compounds were identified on the basis of chemical and spectral data. Two new compounds were elucidated to be 6-deoxyneomajucin (1) and 2-oxo-6-deoxyneomajucin (2), along with nine known compounds 6-deoxypseudoanisatin (3), pseudoanisatin (4), anisatin (5), pseudomajucin (6), protocatecheuic acid (7), shikimic acid (8), shikimic acid methylester (9), beta-sitosterol (10) and daucosterol (11). Compounds 1 and 2 are new majucin-type sesquiterpene lactones.
Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Illicium ; chemistry ; Lactones ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Plants, Medicinal ; chemistry ; Sesquiterpenes ; chemistry ; isolation & purification ; Shikimic Acid ; chemistry ; isolation & purification ; Sitosterols ; chemistry ; isolation & purification ; Spiro Compounds ; chemistry ; isolation & purification