Bone and Bones ; blood supply ; Tissue Engineering

Aim To explore the protective effect of SMT on acute cerebral infarction.Methods Focal cerebral infarction was induced by photochemistry.The expression of iNOS was detected in microglia by double fluorescent immunohistochemistry and laser scanning confocal microscopy. NO content at post-ischemic time points was determined by electron spin resonance(ESR) technique and standard addition method.TTC stain was used to determine the infarct volume.Results At ischemic early period,compared with control group,NO content was not remarkably different after 2 h,6 h of ischemia in the treatment groups,infarct volumes also were not obviously changed.At ischemia mid and late period 12 h,24 h,48 h,the expression of iNOS and NO content was suppressed by SMT treatment in microglia.In TTC stain results,infarct volumes were increased obviously after 12 h,and achieved the peak after 24 h,48 h in control groups,infarct volumes in treatment groups were significantly decreased after 12 h,24 h,48 h.Conclusion SMT may play a protective role in acute cerebral infarction by reducing the expression of iNOS and NO content in microglia.

Objective: To make an impersonal evaluation on the effect of TCM on main biochemical indexes of non-alcoholic fatty liver disease (NAFLD),including ALT,AST,GGT,TC,TG,and HDL-C.Methods: We searched all the randomized controlled trials (RCTs) of TCM for NAFLD from CBM, CNKI,and VIP (1999-2008).RevMan4.2 was used in the analysis. Results: 11 RCTs,including 1078 patients,met the inclusion criteria.All of the trials were not adequate in methodological quality. None of them was double-blinded trial.The result of analysis showed that compared with basic Western medicine,TCM could increase the content of HDL-C,reduce the content of ALT,AST,GGT,TC and TG.Conclusion: The present meta-analysis suggested that TCM was beneficial over Western medicine for NAFLD which indexes including ALT,AST,GGT,TC,TG,and HDL-C.But we can not make a final conclusion due to low quality of RCT.

Objective Identification of viral replication in patients with HB without HBeAg is significant for estimating course and adopting appropriate therapy.This research aimed to clarify clinical signification of detecting Pre-S1Ag for identifing viral replication in patients with HB without HBeAg.Methods Sera samples were collected from 65 outpatients with HB confirmed by liver biopsy. HBV markers ,Pre-S1 antigen in sera were detected by ELISA;HBV-DNA by PCR;HBsAg, HBcAg and Pre-S1Ag in tissue by immunohistochemistry method.Results Intracellular HBsAg,Pre-S1Ag mainly expressing in the plasma account for 78.4% (51/65) ,69.2%(45/65) respectively, HBcAg partly in the plasma and the nucleus account for 67.6(44/65).Positive rate of expressing HBcAg,Pre-S1Ag are little less than HBsAg, but very near between Pre-S1Ag and HBcAg.There are no significant difference by ?~2 test (P=0.736). There are no significant difference between HBV-DNA (≥10~3copies/ml) and Pre-S1Ag(P=0.059),but there are significant difference HBV-DNA-positive are 18, in which 14 are Pre-S1Ag-positive.It demonstrates that there are viral replication in 18 of 30 HBeAg-negative patients and Pre-S1Ag corresponds considerably with HBV-DNA. Among 14 HBeAg-negative patients there are 4 cases with Pre-S1Ag without HBeAg because of Pre-C mutation.Conclusion Pre-S1 is a valuably serological marker for HBV replication without HBeAg.

OBJECTIVE To explore the antagonistic effect of calpain inhibitor Ⅲ MDL 28170 on acute methylmercury (MeHg) neurotoxicity. METHODS The rats were randomly divided into MDL 28170 (50 mg·kg~(-1),ip) control group, MeHg (10 mg·kg~(-1),ig), and MeHg (10 mg·kg~(-1),ig)+MDL 28170 (50 mg·kg~(-1), ip) group. The escape latencies were observed by Morris water maze test. The neurons of positive μ-calpain were determined by immunohistochemical method, the expression of μ-calpain was analyzed by Western blotting, apoptotic neurons were observed by TUNEL method, and microtubule-associated protein 2 (MAP2) expression was detected in neuron by immunohistochemical method. RESULTS MeHg was ig given for 3-7 d, the initial signs of behavioral changes developed in MeHg group, the escape latencies were lengthened in Morris water maze test (P<0.01). The expression and activity of μ-calpain elevated obviously in parietal cerebral neurons(P<0.01), neuronal apoptosis was markedly increased(P<0.01), and the decomposition of MAP2 was reduced in cortical neurons obviously. And the above changes were suppressed obviously by treatment of MDL 28170. CONCLUSION μ-Calpain may correlate with neuronal apoptosis induced by MeHg, MDL 28170 markedly reduces neuronal apoptosis, which might act as therapeutical method for MeHg poison.

AIM:To explore the regulating mechanism of microglia transplantation in treating cerebral infarction.METHODS:Focal cerebral infarction model was established by photochemistry. The histological and immunohistochemical methods were used to observe the effects of microglia transplantation on cerebral infarction. The expressions of nerve growth factor(NGF) and interleukin-10(IL-10) were detected by double fluorescent immunohistochemistry and Western blotting.RESULTS:Microglia marked with green fluorescence was observed in ischemic penumbra,indicating that microglia can be transplanted across the blood-brain barrier. Infarct volumes and death number of cells were significantly reduced compared to non-transplantation animals. The expressions of NGF and IL-10 were markedly increased in microglia in transplantation group.CONCLUSION:Microglia can be transplanted across the blood-brain barrier. The cells have protective effects in ischemic penumbra by secreting NGF and IL-10,which might serve as therapeutical method for treating cerebral infarction.

Objective To explore the relationship between chronic tonsillitis and allergic purpura.Methods Fifty-six allergic purpura children with chronic tonsillitis were selected,26 children were given tonsillectomy (operation group),and 30 children were not given tonsillectomy (no-operation group).The duration of abdominal pain,duration of erythra,proteinuria negative time,hematuria negative time,antistreptolycin O rite,complement C3 were compared between the 2 groups,and the 24 h urine protein quantitarion and urine red blood cell count in operation group were compared between preoperative and postoperative.Results The duration of erythra,duration of abdominal pain,proteinuria negative time,hematuria negative time in operation group were significantly better than those in no-operation group [(10.58 ±4.22) d vs.(16.70 ±9.68) d,(6.49 ±2.45) d vs.(9.20 ± 2.21) d,(12.3 ±4.1) d vs.(16.5 ± 5.6) d,(10.1 ± 3.3) d vs.(12.7 ± 4.9) d],there were statistical differences (P ＜ 0.01 or ＜ 0.05).There were no statistical differences in antistreptolycin O tite and complement C3 between the 2 groups (P＞ 0.05).In operation group,the 24 h urine protein quantitation and urine red blood cell count postoperative were significantly better than preoperative [(0.72 ± 0.28) g vs.(1.61 ± 1.22) g,(14.95 ± 4.76) / μ l vs.(56.61 ± 12.57)/μ 1],there were statistical differences (P＜ 0.01).Conclusion Chronic tonsillitis is one of the important factors which can make allergic purpura erythra relapses and induce purpura nephritis,and tonsillectomy is the optional means of treatment of purpura nephritis with chronic tonsillitis and erythra relapses.

The effects of polysaccharidical peptides (PSP) on the functions of tumour infiltrating lymphocytes (TILs) in vitro was explored. The results showed that PSP, at the concentration of 37-1200?g/ml, promoted the proliferation of TILs in dose-related manner. Combination of PSP with interleukin-2 (IL-2) enhanced the cytotoxicity of TILs and reduced the dose of IL-2 used for activating TILs. The results suggested that PSP could augment the proliferation and cytotoxicity of TILs in vitro.

AIM: To present the epidemiology, cause of injury, ocular status and final visual acuity after management of severe ocular trauma required hospitalization during 7 years in a representative urban Chinese population.METHODS: A retrospective analysis of the hospital admission files of ocular trauma patients admitted to the Daping hospital from January 2000 to December 2006 was carried out.RESULTS: A total of 268 patients were open-globe injury and the remaining 294 patients were closed-globe types. The most common causes of ocular injuries were metal (29.4%), explosive (14.6%) and stone (13.9%). And the visual outcomes of most of eye injury patients in this study were poor; half of injured eyes ended with visual acuity worse than 0.1.CONCLUSION: Therapeutic methods to ocular trauma make a great progress in recent years, but the visual outcomes are poor.

Posterior scleritis,an inflammatory disease of the posterior sclera,is most commonly seen in middle-aged women and often associated with systemic immunological diseases.It presents with a variety of nonspecific signs and symptoms,and its clinical feature may be confused with ocular tumors,orbital inflammation,and retinochoroidopathy.Therefore,some auxiliary examinations are necessary for the diagnosis of posterior scleritis.With early proper diagnosis and treatment,severe complications of posterior scleritis can be avoided or lessened.In the paper,some advancement in the study of etiology,incidence,signs,symptoms,auxiliary examination,differential diagnosis and treatment of posterior scleritis were reviewed.