1.Nicardipine vs labetalol in treating acute and severe hypertension
Chinese Journal of New Drugs and Clinical Remedies 2001;20(3):182-184
AIM: To compare the therapeutic effects and adverse reactions of nicardipine and labetalol injection in treating acute and severe hypertensions. METHODS: Sixty-seven patients in nicardipine group (M 46, F 21; age 52 a± s 11 a) were given nicardipine 2 mg in 0.9 % sodium chloride or 5 % glucose injection 10 mL within 2 min iv, first, and then given 30 mg nicardipine in 0.9 % sodium chlocide or 5 % glucose injection 250 mL by continual intravenous infusion for 6 h. Sixty-one patients in labetalol group (M 41, F 20; age 53 a±9 a) received 50 mg labetalol in 5 % glucose injection 20 mL by intravenous once. The treatment could be repeated every 15 min, in case it was less effective, but the total dose should be no more than 200 mg. RESULTS: The treatment for the nicardipine group and the labetalol group, reached the marked effect within 5 min. The total marked effective rate was all 100 % (P>0.05). Severe adverse reactions occured in the two patients (one occured significantly lowering of blood pressure, and the other aggravated asthma) in the labetalol group. CONCLUSION: Intravenous nicardipine and labetalol are fast and smooth in reducing the blood pressure of the patients with acute and severe hypertension, but the safety of nicardipine is superior to that of labetalol
2.In vitro study evaluating the effect of different subgingival root exposure methods and ferrule designs on fracture resistance of residual root.
Qingfei MENG ; Lijuan CHEN ; Jian MENG
West China Journal of Stomatology 2014;32(1):75-79
OBJECTIVETo evaluate the effect of different subgingival root exposure methods, namely, crown lengthening or forced eruption, and different ferrule lengths on fracture resistance of a residual root restored with a carbon fiber post-and-core system.
METHODSFifty-six extracted endodontically-treated mandibular first premolars were sectioned 1.0 mm coronal to the buccal cementoenamel junction. All the models were divided randomly into seven groups that each consist of eight roots. Group A was given non-ferrule as control. Simulated crown lengthening was performed for the dentin ferrule design in the cervical tooth structure for Groups B, C, and D with a ferrule length of 1.0, 2.0, and 3.0 mm, respectively. Simulated forced eruption was performed with a ferrule length of 1.0, 2.0, and 3.0 mm in the cervical tooth structure for Groups E, F, and G, respectively. After restoration with prefabricated carbon fiber post-and-core system, each specimen was embedded in a self-cured acrylic resin block from 2.0 mm apical to the margins of a cast Ni-Cr alloy crown, then loaded at 150 degrees from the long axis in a universal testing machine at a crosshead speed of 1.0 mm min(-1) until fracture. Data of failure loads and fracture mode were recorded and analyzed.
RESULTSMean failure loads for Groups A to G were (1.13 +/- 0.15), (1.27 +/- 0.18), (1.02 +/- 0.11), (1.05 +/- 0.12), (1.63 +/- 0.14), (1.92 +/- 0.19), and (1.93 +/- 0.15) kN, respectively. The effects of root exposure method and ferrule design differed significantly, and two factors exhibited significant interaction (F=33.396, P<0.0001). When the ferrule lengths were the same, fracture loads in the simulated forced eruption groups were significantly higher than those of the simulated crown lengthening groups (P<0.001). No statistical differences in fracture mode were observed among all groups (P=1.00).
CONCLUSIONMore than 1.0 mm ferrule placement on the apical crown margin by using forced eruption significantly increases fracture resistance.
Bicuspid ; Crowns ; Dental Stress Analysis ; Humans ; In Vitro Techniques ; Post and Core Technique ; Tooth Fractures ; Tooth Root
3.Research progression of ?-SYN in Cancer
Journal of International Oncology 2006;0(11):-
?-Synuclein (?-SYN ) .previously identified as a breast cancer specific gene, has a wildly tumor expression profile mainly in advanced stage. As a special chaperone,?- SYN stimulates the proliferation and metastasis of tumor cells through interacting with estrogen receptor and (or) BubR1. Meanwhile, it is also detectable in the serum or urine from tumor patients, which indicates that?- SYN could be potential as a tumor marker for diagnosis and prognosis.
4.Correlation analysis between outcome of exophthalmos and curative effect of hyperthyroidism in Graves disease after 131I therapy
Liang YIN ; Jian TAN ; Zhaowei MENG
Chinese Journal of Postgraduates of Medicine 2012;35(1):33-36
ObjectiveTo analyze the association between the outcome of exophthalmos and the curative effect of hyperthyroidism in Graves disease after 131I therapy.Methods Five hundred and thirty-eight cases of Graves disease accompanied with exophthalmos and hyperthyroidism who received 131I therapy from January 2001 to January 2010 were reviewed retrospectively,and the association between the outcome of exophthalmos and the curative effect of hyperthyroidism was analyzed.ResultsThe effective rate of hyperthyroidism after 131I therapy was 94.8% (510/538).The effective rate of exophthalmos after 131I therapy was 73.2%(394/538).And the effective rate of hyperthyroidism with exophthalmos was 71.6% (385/538).The incidence of improvement,inefficiency,exacerbation was 75.5% (385/510),21.0% (107/510),3.5%( 18/510) in hyperthyroidism effective patients ( 510 cases ) and 32.1% ( 9/28 ),50.0% ( 14/28 ),17.9% ( 5/28 )in hyperthyroidism ineffective and recrudescence patients(28 cases),there was significant difference between the two patients (P < 0.05 ).The incidences of improvement,inefficiency,exacerbation were 78.1%(281/360),18.9%(68/360),3.1%(11/360) in hyperthyroidism cured patients(360 cases),78.2%(36/46),17.4% (8/46),4.3% (2/46) in hypothyroidism patients(46 cases),there was no significant difference between the two (P > 0.05 ).The incidence of improvement,inefficiency,exacerbation was 86.4% ( 229/265 ),9.8%(26/265 ),3.8%( 10/265 ) in mild exophthalmos after 131I therapy(265 cases),59.9%(106/177),35.6%(63/177),4.5%(8/177) in moderate and severe inactive exophthalmos after 131I therapy( 177 cases),there were significant differences in the inciclence of improvement and inefficiency between the two patients (P<0.05).For patients with moderate and severe active exophthalmos,glucocorticoids therapy could obtain satisfactory effect.Conclusion131I therapy is an effective method for Graves disease with exophthalmos,and there is a significant association between the outcome of exophthalmos and the curative effect of hyperthyroidism.
5.Relationship between body mass index and left ventricular structure and function in young or middleaged men
Juanjuan MENG ; Jingfa TIAN ; Jian LIU
Chinese Journal of Health Management 2011;05(6):330-333
Objective To investigate the relationship between body mass index(BMI)and cardiac structure and function of adult men living in Hebei province.Methods A total of 160 adults were assigned to 4 groups by BMI:normal body weight group(group A,BMI 18.5 to 23.9 kg/m2),overweight group (group B,BMI 24.0 to 27.9 kg/m2),mild obese group(group C,BMI 28.0 to 29.9 kg/m2),and severe obese group(group D,BMI ≥ 30 kg/m2).Left ventricular end-diastolic diameter(EDD),end-systolic diameter(ESD),inter ventricular septal thickness(IVS),left ventricular posterior wall thickness (LVPW),left ventricular mass(LVM),and left ventricular mass index(LVMI)were examined by echocardiography.Early(E)peak diastolic and late(A)diastolic mitral inflow velocity and E/A ratio were measured by Doppler echocardiography.Pulsed wave Doppler tissue image(PW-DTI)of the lateral mitral annulus was performed to evaluate early peak diastolic velocity(Em),late peak diastolic velocity(Am)and Em/Am ratio.Results Compared with group A,EDD,ESD,LNM,LVMI,Am,IVS and LVPW were increased in the other 3 groups(all P < 0.05).E,E/A ratio,Em,and Em/Am ratio were decreased in group B,C,and D(all P < 0.05).Compared with group B,Sm was decreased in group C and D(both P < 0.05).Conclusion Overweight and obesity could contribute to ventricular hypertrophy and deteriorate diastolic function in a BMI dependent manner.Obesity may be also related to decreased Sm.
6.Percutaneous intracystic steroid injection using two needles for solitary bone cysts in children
Jian LU ; Nanfang MENG ; Weisheng WEI
Chinese Journal of Minimally Invasive Surgery 2001;0(02):-
Objective To investigate the effectiveness of percutaneous intracystic steroid injection using two needles in the treatment of solitary bone cysts in children. Methods A total of 28 children with solitary bone cyst underwent steroid injection from January 1996 to January 2004. Under fluoroscopy, two fine needles (either bone marrow biopsy needle or lumbar puncture needle) pierced the bone cyst by way of the top and the bottom of the cyst, respectively. The intracystic fluid was drawn off, the cyst cavity irrigated and the steroid injected into. Results Follow-up checkups for 10~62 months (mean, 28 months) in 27 children found no complications. According to the Chigira classification on the new bone formation, 20 cases were classified as grade Ⅳ, 5 case grade Ⅲ, 1 case grade Ⅱ and 1 case grade Ⅰ, the cure rate being 92 6% (25/27). The time for bone cysts to grow together again in the 25 children was 3~10 months (mean, 4 5 months). Conclusions Percutaneous intracystic steroid injection using two needles for solitary bone cysts in children is simple, safe and effective.
7.The Efficacy of Invasive and Non-invasive Mechanical Ventilation in Treating Severe Respiratory Failure with Chronic Obstructive Pulmonary Diseases.
Minghui DU ; Yuhong MENG ; Jian WEI
Journal of Medical Research 2006;0(02):-
Objective To discuss the efficacy and relative factors that affect the sequential therapy of invasive and non-invasive mechanical ventilation in treating severe respiratory failure.Methods We analyzed the clinical data of the patient who was hospitalized in Respiratory Department of the Sixth Hospital of Luohe on 3th February 2008.Results The patient received invasive mechanical ventilation for 4 days,then changed to mask non-invasive positive pressure ventilation for 10 days,while receiving comprehensive treatment of anti-infection and bronchial expansion,etc.Then the patient was recovered.Conclusion The sequential therapy of invasive and non-invasive mechanical ventilation in treating severe respiratory failure is effective.The efficacy depends on the optimum converting time between invasive and non-invasive mechanical ventilation,the choice of ventilator and the patient′s compliance.
8.Synergistic effects of nuclear factor-kappa B inhibition by small interferece RNA on 131I therapy in differentiated thyroid cancer cells
Yajing HE ; Zhaowei MENG ; Jian TAN
Chinese Journal of Nuclear Medicine and Molecular Imaging 2013;(3):207-212
Objective To study the effect of nuclear factor-kappa B (NF-κB) inhibition by small interference RNA (siRNA) on the apoptosis of DTC cells treated by 131 I.Methods DNA binding assay was performed at 24 h after 131I treatment (2 × 104 MBq/L) on KTC-1 cells.The cell survival assay was conducted at 48 h after 131 I treatment.Western blot was used to detect the changes of NF-κB p65 at 6 h after 131I treatment,and the changes of anti-apoptotic factors and apoptotic key factors at 24 h after 131 I treatment.The anti-apoptotic factors included in this study were X chromosome-linked inhibitor of apoptosis (XIAP),cellular inhibitor of apoptosis 1 (cIAP1) and B-cell lymphoma extra large (Bcl-xL),and the apoptotic key factors were caspase 3 and poly-ADP-ribose polymerase (PARP).A total of 4 groups were studied for the detection of p65 and anti-apoptotic factors by Western blot:no oligonucleotide transfection control group (A),no oligonucleotide transfection + 131I group (B),scrambled oligonucleotides transfection + 131I group (C) and p65 siRNA transfection + 131I group (D).Another 6 groups of studies were:oligonucleotide transfection control group (1),scrambled oligonucleotides transfection group (2),p65 siRNA transfection group (3),no oligonueleotide transfection + 131I group (4),scrambled oligonucleotides transfection +131I group (5) and p65 siRNA transfection + 131I group (6).One-way analysis of variance and q test were performed for statistical analysis.Results The results of DNA binding assays for the 6 groups (1,2,3,4,5,6) were (100.00 ± 11.65)%,(96.00 ± 17.98)%,(9.28 ±5.01)%,(322.72 ±50.81)%,(311.36 ±44.81)% and (36.96 ± 15.66)%,respectively (F =137.74,P <0.01).NF-κB functions were strengthened with 131 I treatment (qgrouo 4∶1 =10.90,qroup 5∶2 =11.38,both P < 0.01).However,NF-κB p65 siRNA transfection could inhibit NF-κB functions (qgroup1∶3 =18.25,qgroup4∶6 =13.71,both P <0.01).Cell survival rates of the 6 groups were (100.00 ± 11.65)%,(96.32 ± 9.44)%,(70.88 ±7.41)%,(64.16 ±9.50)%,(62.24 ±9.37)% and (28.64 ±6.74)% (F=52.76,P<0.01).There were significant differences between groups 3 and 6,groups 4 and 6 (q =10.76 and 7.79,both P < 0.01).Western blot results showed that the expression of NF-κB p65 in the 4 groups (A,B,C,D) were (56.60 ±7.37)%,(111.07 ± 13.31)%,(113.16± 15.04)% and (12.46 ±2.74)%,respectively (F=60.17,P < 0.01).The t65 levels increased with 131 I treatment (qgroup B∶A =6.20,qroup c∶ A =5.85,both P <0.01); while decreased significantly using NF-κB p65 siRNA transfection (qgroup B:D =-12.57; qgroupC∶D =11.41,both P < 0.01).Western blot results showed that XIAP,cIAP1 and Bcl-xL in the 4 groups were (17.59±1.96)%,(16.45± 1.85)% and (19.92 ±2.22)%; (98.37± 17.92)%,(109.81 ±19.16)% and (95.59 ±22.20)% ; (98.43 ±18.71)%,(98.86± 15.88)% and (100.99 ±21.70)% ;(7.00 ± 0.95) %,(5.86 ± 0.35) % and (9.52 ± 0.90) %,respectively (F =44.22,56.51 and 29.11,all P < 0.01).131 I treatment induced higher expression of all the 3 genes (qgroup B∶ A =7.76,8.40 and 5.88,all P <0.01),while NF-κB p65 siRNA transfection,on the contrary,reduced the expression of all the 3 genes (qgroupB:D =8.82,9.40 and 6.71,all P <0.01).There were significant differences of p19,p17,p116 and p89 in the 6 groups(F =39.03,48.45,32.56,52.20,all P < 0.01),especially among group 3,4 and 6 (q =3.18-9.98,all P < 0.05).Conclusions 131I could activate NF-κB function and enhance the expressions of anti-apoptotic factors.NF-κB p65 siRNA transfection could effectively suppress this effect and therefore magnify 131I induced apoptosis in DTC cells.
9.Qualitative research on status of residents' living condition in Third Grade Class A hospitals in Beijing
Guangyuan JIAO ; Meng ZHAO ; Jian ZHANG
Chinese Journal of Medical Education Research 2017;16(2):189-193
Objective By exploring the state of residents' living condition, it is helpful to promote the living condition of residents and improve the quality of medical services. Methods A qualitative re-search was conducted on 13 residents in 4 hospitals in Beijing by depth interview during April to May 2016, and the data were collected and analyzed by using Colaizzi seven step method. Results In terms of work, the residents had specific work, and the interpersonal relationship was more harmonious. In learning, residents were able to take the initiative to learn, and hoped to get more help in scientific research. In life, residents faced multiple pressures from the economy and the family. In the subjective cognition, the cogni-tion of resident was rational and objective. Conclusion The residents' work shows a heavy workload, and while working the residents need to take the study and the life pressure into account. And the residents can rationally recognize their role. To improve the living status of the resident physician, we need to integrate multiple forces, to promote the integration of live doctors into the hospital culture, and enhance their sense of existence. We also need to improve the construction of the system and improve the protection of live training physicians to enhance their sense of security. Most important of all, the whole society should pay more attention to the living condition of the residents and enhance their sense of access.
10.The role of nuclear factor-κB pathway on carcinogenesis and therapy of thyroid cancer
Zhaowei MENG ; Qiang JIA ; Jian TAN
Chinese Journal of Nuclear Medicine and Molecular Imaging 2014;34(5):415-418
The incidence rate of thyroid cancer is increasing very rapidly during the past years.131I treatment for DTC is an effective method.However,DTC refractory to 131I treatment or therapeutic failure is not uncommon.High level expression of nuclear factor-kappa B (NF-λB) in thyroid cancer is closely related with carcinogenesis,progression,anti-apoptosis and therapeutic resistance.NF-κB inhibitor was effective for the treatment of thyroid cancer.Combined NF-κB inhibitor with131I may improve the therapeutic efficacy.