Objective To investigate effects of Livin antisense oligodeoxynucleotides(ASODN) on the proliferation and apoptosis of human leukemia(HL60) cells.Methods Livin protein on HL60 cells was examined by immunohistochemistry.Specific phosphorothioate antisense oligodeoxynucleotides and missense oligodeoxynucleotides target Livin mRNA were synthesized and transfected into HL60 cells following cationic liposome.The proliferation inhibition of HL60 cells was assessed by MTT.The expression of Livin mRNA was detected by RT-PCR.Transmission electron microscope and TUNEL technology were used to detect the apoptosis and morphologic change.ResultsASODN of 600 nmol/L inhibited the HL60 cell proliferation and the expressions of Livin mRNA.The percentage of apoptosis detected by TUNEL was 38.48%?4.37%.cellar ultrastructure was markedly destroyed by Livin ASODN.A significant difference was found when compared with the control group(P

Objective: To investigate the effect of antisense oligodeoxynucleotide (ASODN) of Livin mRNA on the proliferation and apoptosis of human lung adenocarcinorna A549 cells. Methods: Livin ASODN was transfected into A549 cells mediated by cationic liposome. The proliferation rates of A549 cells were assessed by MTT method. The transcription of Livin mRNA was detected by RT-PCR. The Livin protein expression was determined by immunohistochemistry and confocal laser scanning microscopy before and after transfection. The apoptotic ratios of A549 cells were examined by acridine orange/ethidium bromide(AO/EB) fluorescent staining. Results: It was observed that A549 cells expressed both Livin α and Livin β simultaneously, and the distribution of Livin protein could be observed in both cytoplasm and nuclei. The proliferation of A549 cells was inhibited significantly by Livin ASODN in a dose-dependent manner (P<0.01). After transfection of Lip-ASODN at a final concentration of 400 nmol/L, the expressions of Uvin were inhibited at mRNA and protein levels and the apoptotic ratio reached (31.25 ± 5.75)%. The difference was significant compared with control group [ (3.23% ± 1.98)%, P<0.01]. Conclusion: Livin ASODN significantly down-regulates the expression of Livin mRNA and effectively inhibites the proliferation and induces the apoptosis of A549 cells. Therefore Livin gene may become a new target for gene therapy for lung cancer.

dy for public hospitals.Discussions in relation were made on these subjects in an effort to offer references for the financial subsidy system for public hospitals.

Bioreactors ; Humans ; Liver Failure, Acute ; therapy ; Liver, Artificial ; classification ; Sorption Detoxification ; instrumentation ; methods

Adenocarcinoma, Mucinous ; pathology ; Adult ; Aged ; Chondrosarcoma ; pathology ; Chordoma ; pathology ; radiotherapy ; surgery ; Combined Modality Therapy ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; pathology ; radiotherapy ; surgery ; Neoplasm Recurrence, Local ; Paranasal Sinus Neoplasms ; pathology ; radiotherapy ; surgery ; Retrospective Studies ; Sphenoid Sinus

Air ; analysis ; Chromatography, High Pressure Liquid ; methods ; Polyamines ; analysis ; Workplace

Adolescent ; Alopecia ; diagnosis ; therapy ; Bone and Bones ; abnormalities ; Denture, Complete, Upper ; Diarrhea ; diagnosis ; therapy ; Female ; Humans ; Spasm ; diagnosis ; therapy

Purpose:To study the effect of insulin-like growth factor 1 receptor ?-strain(IGF1R-?) interrupted by a special antibody (IGFⅠR-?Mab)on lung adenoma cell line SPC-A-1.Methods:IGFⅠR-?Mab was extracted by hybrid technology. SPC-A-1 cells were separated into 2 groups,the IGFⅠR-?Mab and the blank control. The IGFⅠR-?Mab cells were interfered by different densities of IGFⅠR-?Mab, including 20,40,60,80,100,120,140,160,180 and 200 ng/ml. The MTT curve line, morphology, ultrastructure and cell cycles were observed at 0,24,48,72 hours after the intervention respectively. Results:Compared with the control, apoptosis in IGFⅠR-?Mab group was significant(P= 0.009)and proliferation rate was decreased obviously within 160 ng/ml. However, the proliferation rate was no significant when the special antibody density was more than 200 ng/ml.Conclusions:The affinity of IGFⅠR-?Mab at IGF1R ?-strain is high. The interruption of IGF1R ?-strain by IGFⅠR-?Mab shows the obvious biological effects in vitro ,with inclusion of promoting apoptosis and suppressing proliferation, which indicate the interruption targeting IGF1R ?-strain is prospective for non-small-cell lung carcinoma.

OBJECTIVE:To provide references for how to enhance industrial technical level and research&development ability.METHODS:The status quo of the research&development and the investment of the multinational pharmaceutical enterprises in China,and the motive and impact of their increased investment in China were studied and analyzed.Some countermeasures and recommendations in coping with the international economical situation were presented.RESULTS&CONCLUSION:Under the situation of increasing investment on research&development in china by multinational pharma-ceutical enterprises,we should take advantage of the positive effects and avoid the negative effects,insist on independence and innovation meanwhile enhance international cooperation so as to promote the development of Chinese pharmaceutical indus-tries.

Objective To evaluate the accuracy of bispectral index(BIS)and entropy index in monitoring the depth of anesthesia in patients during target-controlled infusion(TCI)of propofol on induction of anesthesia.Methods Fifty ASA grade Ⅰ-Ⅱ of chronic sinusitis patients who performed the surgery of nasal sinus patency were enrolled in this study.After into operation room(T0),anesthesia was induced with TCI of propofol,and it was added 0.3 μ g/ml after 30 seconds once the plasma drug level was 2.1 μ g/ml(T1)until loss of consciousness(T2),and added 0.5 μg/ml(T3).When tracheal intubation,the patients was injected 0.6 mg/kg rocuronium in their intravenous at the prospective plasma drug level(T4).Each case was monitored with BIS,state entropy index(SE)and response entropy index(RE).The data at following time were recorded:T0-T4,tracheal intubation(T5),1 minute and 3 minutes after tracheal intubation(T6,T7),skin incision(T8).Results The value of BIS,SE and RE were significantly decreased compared with T0 (P ＜0.05).Mean arterial pressure(MAP)and heart rate were in normal range.The value of RE was significantly higher than SE at all the time points(93 ± 9 vs.87 ± 5,88 ± 12 vs.82 ± 12,73 ± 25 vs.72 ± 21,57±21 vs.56±22,46± 16vs.43 ± 17,39± 14 vs.37± 12,36± 14vs.34± 11,35 ± 11 vs.32±9,39±15 vs.36 ± 12)(P ＜ 0.05),but there was no significantly difference between BIS and SE at all the time points(P ＞ 0.05).The value of BIS had significantly positive correlation with SE and RE(r =0.887,0.901 ;P ＜ 0.01).Conclusions During deep hypnosis,BIS,SE and RE all can provide information about the level of consciousness during TCI of propofol on induction of anesthesia.RE is more preponderant as a monitor than BIS and SE.