Objective:To assess the efficacy and tolerability of sibutramine as a weight reduction agent in treatment of overweight patients with type 2 diebetes.Methods: Forty overweight patients(body mass index ≥25 kg/m~(2)) with type 2 diebetes were randomized into sibutramine treatment group and control group(n=20).Both groups received a reduced-calorie diet(deficit of 1 045-2 090 kJ/d from an average daily intake) and continued their original treatment;sibutramine treatment group was also given sibutramine 15 mg once daily for 12 weeks.Before and after sibutramine treatment,body mass index(BMI),fasting blood glucose(FBG),fasting serum insulin(FINS),hemoglobin A_(1C)(HbA_(1C)),and serum lipids were measured in the patients.Homeostasis model assessment(HOMA) was applied to assess the status of insulin resistance(IR).Results: After treatment,BMI,FBG,HbA_(1C),_()TG,FINS and HOMA-IR all decreased(P

OBJECTIVE:To analyze and identify the liposoluble constituents in Isatis indigotica.METHODS:The liposoluble constituents in Isatis indigotica were extracted with petroleum benzin,and the constituents in methyl ester products were sperated,identified and determined by GC/MS.RESULTS:A total of 42 liposoluble compounds were detected,with fatty acid methyl esters making up 36.72%(saturated fatty acid methyl esters 8.67% and unsaturated fatty acid methyl esters 28.05%),and of which,35 were reported for the first time in the study of Isatis indigotica.CONCLUSION:The liposoluble compounds in Isatis indigotica serve as reference for its comprehensive benefits and development of its antioxydant applilication.

Objective　To observe the effect of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line. Methods　The effect of drugs on the cells was measured by MTT, and the protein expression of cyclin D1 gene in the cells was measured by immunohistochemistry assay. Results　①The anti-tumor effects of drugs on the cells were increased with the increasing of concentrations, and had a combined function between NDGA and MTX or VCR. ②There was no difference between the effect of treating NDGA with MTX or VCR given at the same time and MTX or VCR given after 24 h of NDGA treatment, but the effects were apparently different when NDGA was administrated after MTX or VCR treatment for 24 h. ③The protein expression of cyclin D1 gene in the cells was apparently reduced after NDGA treatment. Conclusion　There is a cooperative function between NDGA and MTX or VCR on the cells, and the function is probably related to the NDGA-induced down-regulation of protein expression of cyclin D1 gene in the cells.

Central post-stroke pain refers to pain resulting from a primary lesion or dysfunction of the central nervous system after a stroke.The prevalence of central post-stroke pain varies from 8％ to 46％.This article reviews the definition,epidemiology,pathophysiology,clinical features,and treatment of central post-stroke pain.

Aim To study the protective effects of Presumptive endotheliumartmendependent hyperpolarizing factor(EDHF)released from the rat middle cerebral arteries(MCA),which was mediated by acetylcholine(ACh),on primarily cultured hippocampal neurons subjected to hypoxia-reoxygenation injury.Methods Primarily cultured hippocampal neurons was insulted by hypoxia-reoxygenation;EDHF was produced in rat MCA ring by 1 ?mol?L-1 ACh in the presence of NG-nitro-L-argininemethyl ester(L-NAME,a NOS inhibitor)and indomethcacin(Indo,a COX inhibitor);MTT absorbance and the LDH activity were served as the cell injury index,The level of free calcium fluorescence intensity in the cultured hippocampal neurons was monitored by laser scanning confocal microsope.Results Compared with nomal group,MTT absorbance were decreased significantly,LDH activity in the supernate culture fluid and the Ca2+ in hippocampal neurons increased significantly in model group.The conjoined use of 1 ?mol?L-1 ACh and the endothelium-intacted MCA(MCA/Endo)or ACh+MCA/Endo+L-NAME+Indo can repress both the decrease of MTT absorbance and the increases of LDH activity in the supernate culture fluid and the Ca2+ in neurons which resulted from hypoxia-reoxygenation injury;Neither 1 ?mol?L-1ACh alone nor MCA/Endo alone has the similar effects mentioned above,the conjoined use of 1 ?mol?L-1 ACh and the endothelium-denuded MCA(ACh+MCA/-Endo)also has little effect.K+ which concentration is between 25~35 ?mol?L-1 can significantly attenuate the effects afforded by the conjoined use of 1 ?mol?L-1 ACh,MCA/Endo,L-NAME and Indo,but it is not the same situation when it comes to Ba2+.Conclusions Presumptive EDHF can protect primarily cultured rat hippocamal neurons insulted by hypoxia-reoxygenation and the mechanism is partially concerned with the inhibition of calcium overload.

AIM: To study the protection effect of baicalin on isoproterenol (ISO) induced myocardial ischemia in rats and its mechanisms. METHODS: Forty-five Sprague-Dawley rats were randomly divided into 3 groups. The rats in control group (n=15) received no treatment. The rats in ISO group (n=15) received two times of subcutaneous injection of ISO (20 mg?kg~ -1), which was separated by a 24 hours-interval. The rats in baicalin group (n=15) received ISO as described above and received baicalin 50 mg?kg~ -1 ip. simultaneously. Two days later the hemodynamic measurement was performed and the serum levels of LDH, CK and TNF-? were detected. The expression of p38mapk in myocardium was detected by immunohistochemical technique. RESULTS: The heart function of the group treated with baicalin was improved significantly compared with that of control group. The levels of LDH, CK and TNF-? in baicalin group decreased significantly compared with those in control group and the expression of p38mapk in myocardium also decreased markedly. CONCLUSION: Baicalin has protection effect on isoproterenol induced myocardial ischemia in rats, and the effect maybe due to the decrease of TNF-? induced by reducing the p38mapk expression.

Purpose To investigate the expression of TAZ and β-catenin in colorectal carcinoma and to analyze their correlation with the clinical pathological features of colorectal carcinoma. Methods The immunohistochemical method was used to detect the expres-sion of TAZ and β-catenin in 168 colorectal carcinoma specimens and 30 normal colorectal mucosal specimens. Results The positive rate of TAZ expression was significantly higher in colorectal carcinoma than that in normal colorectal mucosal (P<0. 01). The ectopic expression ofβ-catenin was significantly higher in colorectal carcinoma than that in normal colorectal mucosa (P<0. 01). The expres-sion of TAZ was correlated with degree of differentiation, lymph node metastasis, invasion depth and TNM stages (P<0. 05), and that of β-catenin was correlated with lymph node metastasis, invasion depth and TNM stages (P<0. 05). The positive expression of TAZ and the ectopic expression of β-catenin were significantly related to the 5-years survival rate ( P<0. 05 ) . The expression of TAZ was significantly positively correlated with the ectopic expression of β-catenin ( P<0. 01 ) . Conclusion Up-regulated expression of TAZ and ectopic expression of β-catenin may be synergistically involved in the occurrence, invasion and metastasis of colorectal carcinoma.

Ultrasonic imaging diagnostic technique is one of the most widely used imaging diagnostic techniques,whose fast development has greatly raised the level of clinical diagnosis.The development of ultrasonic imaging diagnostic technique is mainly introduced and various kinds of ultrasonic imaging diagnostic techniques are mentioned including A-mode,M-mode and B-mode ultrasonic diagnostic techniques,Doppler imaging technique,3D imaging technique and harmonic imaging technique.

AIM: To present the epidemiology, cause of injury, ocular status and final visual acuity after management of severe ocular trauma required hospitalization during 7 years in a representative urban Chinese population.METHODS: A retrospective analysis of the hospital admission files of ocular trauma patients admitted to the Daping hospital from January 2000 to December 2006 was carried out.RESULTS: A total of 268 patients were open-globe injury and the remaining 294 patients were closed-globe types. The most common causes of ocular injuries were metal (29.4%), explosive (14.6%) and stone (13.9%). And the visual outcomes of most of eye injury patients in this study were poor; half of injured eyes ended with visual acuity worse than 0.1.CONCLUSION: Therapeutic methods to ocular trauma make a great progress in recent years, but the visual outcomes are poor.

Objective To discuss the CT features of mediastinal teratoma.Methods 35 patients with mediastinal teratoma confirmed by clinical and pathologic data were retrospectively analyzed.Results Most mediastinal teratomas(97.1%) were localized in the middle or upper area of anterior mediastinum.Mediastinal teratoma included cystic teratoma(60.0%) and solid teratoma(40.0%).All kinds of tissues could be found in the tumors.CT features of cystic teratoma(n=21) were the cystic mass with fluid density,most of them(n=19) had the characteristics of "obviouswalled cysts".CT features of solid teratoma(n=14) were the soft-tissue mass with mixed densities.CT features of malignant teratoma(n=5) were that the layer of fatty tissue between the tumor and the surrounding structures disappeared,and that the wall of neighbour vessels were mostly or all surrounded by the tumor.Mediastinal teratoma could cause pleural offusion(n=6) and pericardial offusion(n=12).Conclusion Mediastinal teratomas have obvious CT imaging features.CT has very important value in the diagnosis and differential diagnosis of mediastinal teratoma.