Adult ; Aged ; Aged, 80 and over ; Cadmium ; blood ; Case-Control Studies ; Coronary Disease ; blood ; Female ; Humans ; Lead ; blood ; Male ; Middle Aged

Adenocarcinoma ; metabolism ; pathology ; Adenoma ; metabolism ; pathology ; Colonic Neoplasms ; metabolism ; pathology ; Cytoplasm ; metabolism ; Female ; HSP27 Heat-Shock Proteins ; Heat-Shock Proteins ; analysis ; Humans ; Intestinal Mucosa ; metabolism ; pathology ; Male ; Neoplasm Proteins ; analysis ; Proteome ; analysis ; Proteomics ; methods ; Rectal Neoplasms ; metabolism ; pathology

Objective To optimize the preparation of high-efficiency galactocylated poly-L-lysine (Gal-PLL) ligand of the asialoglycoprotein receptor in liver, providing premise and foundation for upper preparation of ultrasound contrast agent of liver targeted nanoscale perfluorocarbon microballoon and the liver targeted molecular imaging. Methods Chemical reactions of reductive amination were carried out on group A and group B according to different proportions of reaction component. Each group was subdivided into three subgroups. In group A, three different molar ratios of D-galactose and poly-L-lysine (PLL) were compounded respectively with equivalent and sufficient reductant borohydride. In group B, identical molar ratios of D-galactose and PLL were compounded respectively with three unequal reductants borohydride. Products of each group were separated and purified by sephadex column to acquire different molecular weight distributions and the results were analyzed. Results In the condition of identical reductant, the peak curve of compound's molecular weight appeared earlier when D-galactose decreased properly. In the condition of identical molar ratio of D-galactose and PLL,the peak curve of compound's molecular weight appeared also earlier when reductant decreased properly. When the molar ratio of D-galactose and reductant was 1∶1, the peak curve of compound Gal-PLL and free components was more obvious, and the quantity of compound Gal-PLL increased to maximum. Conclusions In the condition of identical reductant, coupling effect of D-galactose and PLL increased when D-galactose decreased properly. In the condition of identical molar ratio of D-galactose and PLL, coupling effect was better when reductant decreased properly. When the molar ratio of D-galactose and reductant was 1∶1, coupling effect of them was the best. The coupling of D-galactose and PLL was related to not only the proportion of D-galactose and PLL, but also the proportion of D-galactose and reductant.

Child, Preschool ; Fibroma ; pathology ; Humans ; Male ; Oropharyngeal Neoplasms ; pathology

OBJECTIVETo compare differences of therapeutic effect between acupuncture method of tonifying-qi and dredging-meridian and conventional acupuncture method.

METHODSSeventy cases of blepharospasm were randomly divided into an observation group and a control group, 35 cases in each one. Cuanzhu (BL 2), Yuwei (Extra) through Yuyao (EX-HN 4), Xinming 1 (Extra), Sibai (ST 2) and Yintang (GV 29) were selected as main acupoints. The acupuncture method of tonifying-qi and dredging-meridian was applied in the observation group, which was an acupuncture manipulation that could achieve strong needling sensation of qi arrival combined with electric-impulse stimulation. The conventional acupuncture method was applied in the control group. The treatment was given once every other day, 10 times as a session for continuous three sessions in both groups. The severity of blepharospasm was compared before and after treatment in two groups and clinical therapeutic effect was assessed. Follow-up visit was performed after half a year to observe the recurrence rate.

RESULTSThe cured and markedly effective rate was 77.1% (27/35) in the observation group, which was superior to 51.4% (18/35) in the control group (P < 0.05). Among cured and markedly effective patients in both groups, the recurrence rate was 20.0% (4/20) in the observation group, which was inferior to 54.5% (6/11) in the control group (P < 0.05) in the follow-up visit.

CONCLUSIONThe acupuncture method of tonifying-qi and dredging-meridian could effectively improve severity of blepharospasm, which has superior short-term and long-term effects to conventional acupuncture method.

Acupuncture Points ; Acupuncture Therapy ; Aged ; Blepharospasm ; therapy ; Female ; Humans ; Male ; Meridians ; Middle Aged ; Qi ; Treatment Outcome

The risk factor of dental fluorosis are mainly related to the cancen tration of fluoride in the drinking water,the supplements of fluoride,nutrition states and regime,the time of fluoride exposure.Other factor such as the period of mother's milk feeding,the altitude of resident,the environmental pollution,the education degree of parents,and the level of health service of the community are also contributed to the development of dental fluorosis.Besides,the knowledge of dentists,the health habits of individual,the condition of economic,and so on is somehow the risk factor of dental fluorosis.The chances of fluoride exposure will become more often as the prosperous of the society.Thus any health policy of the complement of fluoride should be adapted to local conditions.

Objective　To find out the infection of brucella among local people.Methods　To implement requirement of brucella surveillance program according to the prevention and cure of brucella document.From 1996-1998,continous surveillance of the group people aged 7～60 years was done.Results　Three years surveillance showed that in Yuli county the brucella average infection rate is 13.11%,the rate of contracting disease is 2.62%.Such disease happened among different profession and different kinds of people.It shows that the brucela infection has transferred and diffused from pasturing area to rural section and town.Conclusions　The ways is to adopt the available prevention and cure ways,to avoid the source of brucella infection,to strengthen the prevention and cure of the brucella infection among the animals,to improve people's protection consciousness,to reduce the harm that disease done to people.

Objective To explore the clinical feature,treatment and prognosis of neonatal small intestinal atresia.Methods Retrospective study was carried out of 80 neonatal small intestinal atresias over the last 14 years,including diagnosis,treatment and prognosis.Results Of them,62 cases were healed(77.5%),18 cases were dead(22.5%),24 cases(30%)associated with other anomalities.The living cases were followed up for 6 months to 3 years.These symptoms had not relapse.Conclusions Satisfactry effect can be obtained by early diagnosis and operative treatment.Sonography is an ideal way for early finding intestinal atresias in a fetus.End-to-end single layer anastomosis is an ideal way for monointestinal atresia,and poly-end-to-end single layer anastomosis plus long gastrointestinal silicone tube for supporting is an ideal way for multipal intestinal atresia.

Objective To summarize the experience of nursing cooperation for immediate breast reconstruction with the latissimus dorsi myocutaneous flap. Methods Twenty breast cancer patients underwent modified radical surgery with intraoperative immediate application of the latissimus dorsi myocutaneous flap in combination with the same period breast prosthesis for breast reconstruction. The nursing measures included preoperative preparation and mental support, intraoperative active cooperation, position care, sustaining of body temperature and strict aseptic and disease-free technology. Results All the breast cancer patients lived through breast cancer modified radical immediate breast reconstruction. No flap necrosis occurred , neither did obvious complications. The operations were satisfactory. Conclusion The key to the breast reconstruction for the breast cancer patients exists in preoperative psychological nursing , intraoperative strict aseptic and disease-free technology , the proper operation position and the correct use of instruments and equipment.

Agricultural Workers' Diseases ; Agriculture ; Humans