Objective To discuss clinical effect of bronchodilator treatment on patients with chronic obstruc-tive pulmonary disease.Methods According to the exclusion criteria,106 patients were selected and randomly divid-ed into two groups,the control group were given glucocorticoid therapy,the study group was given glucocorticoid +bronchodilator treatment;lung index,including forced expiratory volume in one second,forced vital capacity,and the percentage of forced expiratory volume in one second of forced vital capacity of the two groups were compared,and the patientˊs clinical curative effect was observed.Results The patients of the study group of three indicators:forced expiratory volume in one second,forced vital capacity,forced expiratory volume in one second of forced vital capacity percentage were (1.6 ±0.8)L,(3.4 ±0.4)L,(48.6 ±2.3)%,which were higher than those of the control group, the differences were statistically significant (P <0.05);The total effective rate of the study group was 96.3%,which was higher than 81.2% of the control group,the difference was statistically significant (P <0.05).Conclusion The clinical effect of glucocorticoid +bronchodilator on patients with chronic obstructive pulmonary disease is good,which can effectively improve patient forced expiratory volume in one second,forced vital capacity,forced expiratory volume in one second percentage of forced vital capacity,and can avoid illness development,significantly improve the treat-ment efficiency.

[Objective] To explore the effects of mycocide and insecticide on indoor air quality in archives.[Methocls] The levels of camphor, formaldehyde and 2, 2-dichlorovinyldimethyl phosphate (DDVP) in indoor airwere determined in 17 archives. [Results] The average concentration of camphor in indoor air was 1.00mg/L inarchives applying synthetic camphor, 0.74 mg/m3 in archives applying commercialized mycocide (the main compo-nents were synthetic camphor and pyrethrin ), and 0.14 mg/m3, applying natural camphor. The concentrations offormaldehyde reached 0.297 mg/m3 at the 10th day after the co-fumigation by formaldehyde and DDVP. At thesametime, DDVP was not found in indoor air of archives.[Conclusion]The pollution of formaldehyde and camphorin indoor air of archives should be paid more attention to.

Objective:To have a research and application on management system of cold chain facilities temperature control in the hospital and real-time monitor the temperature and humidity changes of cold-chain facilities.Methods: Based on RFID radio frequency, Internet of things and computer technology to realize the construction of the system hardware and software platform, through commissioning and case analysis for system improvement, the system was popularized and applied comprehensively.Results: After one year’s trial run, it showed that the system could accurately and effectively monitor the abnormal temperature and humidity of the hospital’s cold chain facilities and give the acousto-optic hint and remote alarm in time, effectively improving the management level of cold chain temperature control.Conclusion: The system could guarantee the fine management of the cold chain facilities temperature control, provide safe and traceable historical data, and put forward a new solution for building the quality and safety of cold chain facilities temperature control system.

Objective To stady the clinical effects of repairing soft tissue defects on body surface by grafting free anterolateral thigh flaps. Methods 11 patients were treated for soft tissue defects on body surface by grafting free anterolateral thigh flaps from October,1986 to January,2002. Results all flaps in the 11 patients survived.Follow-up survey in the (6～18) months after the operation showed satisfactory results. Conclusions Free anterolateral thigh flap is well supplied with blood,and it is in a concealed area of the body,where flaps of large size can be cut off.It is the ideal flap to repair large areas of soft tissue defects on body surface.

The aim of this study is to prepare hyaluronic acid (HA) modified core-shell liponanoparticles (pHA-LCS-NPs) as gene delivery system and investigate its gene transfection efficiency in human retinal pigment epithelium (ARPE-19) cells in vitro. The pHA-LCS-NPs was prepared by firstly hydrating dry lipid film with CS-NPs suspension to get LCS-NPs, then modifying the lipid bilayer with HA by amidation reaction between HA and dioleoyl phosphatidylethanolamine (DOPE). Its morphology, particle size and zeta potential were investigated. XTT assay was used to evaluate the cell safety of different vectors in vitro. The gene transfection efficiency of pHA-LCS-NPs modified with different contents of HA was investigated in ARPE-19 cells with green fluorescent protein (pEGFP) as the reporter gene. The results showed that the obtained pHA-LCS-NPs exhibited a clear core-shell structure with the average particles size of (214.9 +/- 7.2) nm and zeta potential of (-35 +/- 3.7) mV. The 24 h cumulative release of gene from pHA-LCS-NPs was less than 30%. After 48 h incubation, gene transfection efficiency of pHA-LCS-NPs/pEGFP was 1.81 times and 3.75 times higher than that of CS-NPs/pEGFP and naked pEGFP, respectively. Also no obvious cytotoxicity was observed on pHA-LCS-NPs. It suggested that the pHA-LCS-NPs might be promising non-viral gene delivery systems with high efficiency and low cytotoxicity.
Cell Survival ; Gene Transfer Techniques ; Genes, Reporter ; Genetic Vectors ; Green Fluorescent Proteins ; metabolism ; Humans ; Hyaluronic Acid ; chemistry ; pharmacology ; Lipids ; Nanoparticles ; Particle Size ; Phosphatidylethanolamines ; chemistry ; pharmacology ; Retinal Pigment Epithelium ; drug effects ; Transfection

Animals ; Anti-HIV Agents ; pharmacology ; HIV ; drug effects ; genetics ; physiology ; HIV Infections ; drug therapy ; immunology ; virology ; Humans ; Mucous Membrane ; immunology ; virology ; Virus Replication ; drug effects

Animals ; Apoptosis ; drug effects ; Ginsenosides ; pharmacology ; Myocardial Reperfusion Injury ; pathology ; Myocytes, Cardiac ; cytology ; Rats ; Rats, Wistar

Anal Canal ; surgery ; Child ; Child, Preschool ; Colon, Sigmoid ; surgery ; Digestive System Surgical Procedures ; methods ; Female ; Hirschsprung Disease ; surgery ; Humans ; Infant ; Laparoscopy ; Male ; Rectum ; surgery ; Treatment Outcome

Objective To explore the related risk factors for bypass graft occlusion in patients within 1 year after coronary artery bypass grafting,in order to provide the basis for the prevention of postoperative bypass graft occlusion.Methods Clinical data of 197 cases treated with coronary artery bypass grafting were collected,and the incidence rate of bypass graft occlusion and its related risk factors were analyzed.Results Graft occlusion occurred in 28 (14.2％) of 197 patients.The incidence rate of bypass graft occlusion were much higher in patients with BMI ≥24 kg/m2,smoking,hypertension,hyperlipemia,diabetes,peripheral vascular diseases and cerebrovascular disease than in patients with BMI ＜24 kg/m2,non-smoking,patients without hypertension,hyperlipemia,diabetes,peripheral vascular diseases or cerebrovascular disease (20.0％ vs.9.8％,22.4％ vs.10.8％,21.2％ vs.3.8％,31.1％ vs.9.2％,23.4％ vs.9.8％,25.5％ vs.10.7％,26.3％ vs.11.3％,x2 =4.106,4.534,11.735,13.658,6.615,6.486,5.656,respectively,P=0.043,0.033,0.001,＜0.001,0.010,0.011,0.017).Smoking,diabetes,hyperlipidemia,peripheral vascular diseases and cerebrovascular disease were the independent risk factors for bypass graft occlusion.Conclusions Many risk factors are related with short-term postoperative bypass graft occlusion in patients with coronary artery bypass grafting.The corresponding control measures should be conducted to decrease the risk of postoperative bypass graft occlusion,aiming directly at the risk factors,especially the independent risk factors.

Objective To statistically analyze data of blood transfusion from General Hospital of Chengdu Military Command,Mianyang field blood station,Deyang field blood station and other military medical institutions from May 12 to June 30,2008 so as to provided certain references for reasonable blood supportability in wartime and disaster.Methods A statistical analysis was done on data of blood collection and supply including self-taken blood and assembled blood,total amount of blood supply as well as the transfusion information of inpatients injured by earthquake in our hospital.Results The amount of self-taken blood was 5 111 U,the amount of assembled blood 3 380 U and the total amount of blood supply 1 0405.5 U.But blood transfusion was 4 090.6 U in 132 patients admitted into General Hospital of Chengdu Military Command.In addition,the crest-time of transfusion appeared at 96 hours after earthquake.Moreover,patients with fractures received the highest rate of blood transfusion and crush syndrome patients received the most blood transfusion and the highest per capita transfusion.Conclusions Blood supportability in earthquake is different from that in wartime and other disasters in aspects of transfusion time,blood types and blood transfusion volume.It is important to analyze the characteristics of transfusion in patients injured by earthquake for national strategy of blood supportability in disasters and for blood supportability in the wartime.