Objective To investigate the association between polymorphism of S1, S2 locus allele in ADAM 33 gene and chronic obstructive pulmonary disease (COPD) and lung function in Xinjiang Uygur population. Methods Blood sam?ples from 217 COPD patients and 218 healthy controls were collected. Samples of DNA was extracted, and S1, S2 single nu?cleotide polymorphism (ADAM 33) was detected by ABI SNaPshot SNP genotyping. Results There were no significant dif?ferences in the frequencies of S1 locus CC, CT, TT genotypes and C, T alleles between patient group and control group (P>0.05). There were no significant differences in the frequencies of S1 locus CC, CG, GG genotypes and C, G alleles between patient group and control group (P>0.05). In patient group, there were no significant differences in S1, S2 locus genotype and clinical indicators of lung function display, and in the FEV1%predicted and FEV1/FVC (P>0.05). Haplotype analysis showed that there were no significant differences in three kinds of haplotypes between patient group and control group ( P>0.05). Conclusion There is no significant difference in the polymorphism of S1, S2 locus allele in ADAM 33 gene and the susceptibility to COPD in Xinjiang Uygur population.

OBJECTIVETo prepare a monoclonal antibody against hepatoma-specific gamma-glutamyltransferase (GGT-II) and study it's application.

METHODSTwo Bal B/C mice were immunized with pure GGT-II, then their spleen cells were separated and fused to SP 2/0 myeloma cells so as to make hybridoma cell strain which could yield monoclonal antibody against GGT-II. And it's effect of binding GGT-II was detected by competitive inhibitory enzyme linked-immunosorbance assay (ELISA).

RESULTSA mouse hybridoma cell strain which could steadily secrete the monoclonal antibody against GGT-II was obtained and named 2G4F6B2. This monoclonal antibody belonged to IgG1 subclass and was specific to GGT-II, without cross-reaction to GGT-II. The result of detecting human serum GGT-II by ELISA with the monoclonal antibody accorded with that by polyacrylamide gradient electrophoresis.

CONCLUSIONThe monoclonal antibody against GGT-II prepared in this study has high specificity and can be applied in clinic to detect human serum GGT-II.

Animals ; Antibodies, Monoclonal ; Carcinoma, Hepatocellular ; diagnosis ; enzymology ; Enzyme-Linked Immunosorbent Assay ; Female ; Liver Neoplasms ; diagnosis ; enzymology ; Mice ; Mice, Inbred BALB C ; gamma-Glutamyltransferase ; analysis ; immunology