Gastric cancer(GC) is deeply threatening the health of our people, the mortality rate of GC is the second death cause of carcinoma i n our country. Since the lack of special clinical symptom at the early state of GC, detecting GC at the molecular biological level is expected to diagnose early gastric cancer. This article discusses some markers such as PCNA, p53, telomera se, ras protein and so on to assess the possibility of diagnosing the early gas tric cancer with these molecular biological markers. According to analyzing rela ted papers published, PCNA and p53 were found to be the molecular biological mar kers which should be firstly taken into account in diagnosing gastric cancer. Bo th of them can be easily detected by using immunohistochemical method. Telomeras e is also a good marker, but because of its difficult technology and high cost, it is not so practicable as the former two as the diagnostic marker of early gas tric cancer. Besides, ras potein can also be taken into account as the early det ecting auxiliary marker for GC.

Objective: To evaluate the preventive effect of ephedrine on hypotension under spinal anesthesia in patients with different age. Methods: Sixty patients scheduled for spinal anesthesia were randomly allocated to four groups with 15 each. Group Ⅰ and Ⅱ were adult patients aged from 25 to 55 years old and group Ⅲ and Ⅳ were geratic aged patients of 6575 years old. Every patient received a fluid loading of 7ml/kg before spinal anesthesia. The ephedrine was administrated to group Ⅱ and IV patients in a dose of 10mg i v and 30 mg i m and same amount of normal saline was administrated to group Ⅰ and Ⅲ as soon as spinal anesthesia was performed. Results:Thirty patients in the placebo groups developed a maximal decrease in MAP exceeding 20%. Ten of these (2 in group Ⅰ and 8 in group Ⅲ ) developed a decrease in MAP exceeding 35% and required treatment. In aged groups, all patients in group Ⅲ had a decrease in MAP exceeding 20 %, 53 % of whom, the decrease exceeded 35 %. Conclusion:Prophylactic use of ephedrine is effective in the prevention of hypotension during spinal anesthesia, especially in aged patients.

OBJECTIVETo investigate the effect of U-shaped gypsum functional fixation after closed manipulative reduction for treatment of Colles' fractures.

METHODSFrom January 2011 to April 2014,47 cases of Colles fracture were treated by closed reduction and U-shaped gypsum functional fixation including 12 males and 35 females with an average age of 54.8 years old ranging from 8 to 72 years old. The time from injury to treatment was 40 min to 3 d. The patients were closed fractures without neurovascular injury. After manual reduction and U-shaped gypsum functional fixation, the thumb of injury hand were traction by contralateral hand, and other fingers of injury hand were taken flexion to exercise grip function. According to the situation of fracture healing, plaster was removed at 4 to 8 weeks' fixation, the wrist joint functional exercise was strengthened after plaster removed.

RESULTSAll patients were followed up from 6 to 12 months with an average of 6.4 months, the fracture healing time was 4 to 8 weeks. According to the Cooney wrist function scoring: the result was excellent in 44 cases, good in 2 cases, 1 case.

CONCLUSIONU-shape gypsum functional fixation for treatment of Colles fracture limits the activity of thumb and extensor tendon, can reduce shortening of radial by traction of the injury thumb with the contralateral hand; it is good for blood circulation of hand and wrist, and swelling.

Adolescent ; Adult ; Aged ; Child ; Colles' Fracture ; therapy ; Female ; Fracture Fixation ; methods ; Humans ; Male ; Manipulation, Orthopedic ; methods ; Middle Aged

Objective To observe the influence of total flavonoids from Scutellaria baicalensis (TFSB) on Bleomycin-induced pulmonary fibrosis in rats and investigate the relative mechanism. Methods The pulmonary fibrosis model was established by intratracheal injection of Bleomycin. After 28 d, rats with TFSB treatment by ig administration once a day were killed. The levels of T-AOC, GSH, and MPO in serum were measured and analyzed and the fixed parts of the lung tissue were obtained for HE and Masson staining for histopathological examination. The influences of TFSB on the expression of transforming growth factor-β1 (TGF-β1), Smad2, Smad7, α-SMA, and Collagen I mRNA in lung tissue of rats with Bleomycin-induced pulmonary fibrosis were assayed by RT-PCR. Results Compared with the model group, the levels of T-AOC and GSH in serum were dramatically increased and the level of MPO was decreased remarkably after TFSB treatment; The alveolitis and fibrosis extent were attenuated after TFSB treatment (P< 0.05, 0.01), and the expression levels of TGF-β1, Smad2, α-SMA, and Collagen I mRNA were decreased, and the expression level of Smad7 mRNA was increased (P<0.05, 0.01) significantly. Conclusion TFSB could have mitigable effect on Bleomycin-induced pulmonary fibrosis in rats. The mechanisms might be related to the anti-oxidative damage, the inhibition of activation of inflammatory cell infiltration, and the regulation of TGF-β1/Smad signal pathway.

Hepatectomy ; Humans ; Liver Neoplasms ; surgery ; Risk Assessment

Objective: To investigate the effects of thyroid motilin (MTL) on migrating motor complex (MMC) in fasting rats. Methods: The distribution and quantitative expression of motilin in the thyroids were detected by immunohistochemistry and radioimmunoassay. We also observed the effect of thyriod motilin on the MMC by recording the gastrointestinal motility in conscious rats. Results: Tail vein injection of MTL increased MMC in antrum and duodenum in a dose-dependent manner, as manifested by increased contraction frequency and amplitude (P<0.05, P<0.01). However, the contraction frequency and amplitude were significantly decreased after injection of MTL-R antagonist GM109(P<0.05). GM109 could obviously block the promoting effect of MTL on MMC. Removing left thyroid significantly increased MMC cycle, contraction amplitude, and decreased contraction frequency (P<0.05). Injection of anti-MTL serum into the thyroids could significantly promote MMC and increase the frequency and amplitude of contraction (P<0.05). Immunohistochemistry demonstrated that motilin immunoreactive (MTL-IR) cells, together with calcitonin (CT) ,were observed in the thyroids, indicating the expression of MTL in thyroid C-cells. Radioimmunoassay analyses displayed that MTL level in the duodenal mucosa was 128.02 and 100.57 times those in the antrum and thyroid, respectively. Conclusion: MTL is expressed in the thyroid C-cell and participates in the regulation of MMC.

Objective To observe pain and ADL change in patients with osteoporotic vertebral fracture trea- ted by percutaneous kyphoplasty.Methods Percutaneous kyphoplasty were performed in 14 patients with thoracic and lumbar vertebral compression fracture resulted from osteoporosis.All patients were evaluated with regard to pain index and Barthel index before and after operation.Results All the patients'pain were significantly relieved,their score with Barthel index was markedly improved.Conclusion Percutaneous kyphoplasty is a kind of good tech- nique to treat patient with thoracic and lumbar compression fractures resulted from osteoporosis.

Objective To evaluate the surgical procedures and clinical efficacy of endoprosthetic replacement as a salvage procedure for intertrochanteric fracture suffered failure of internal fixation.Methods The study involved 18 intertrochanteric fracture patients that suffered failed internal fixation from 2001 to 2009,including 16 patients with failed dynamic hip screw (DHS) fixation and two with failed proximal femoral nail anti-rotation (PFNA).The patients were at mean age of 76.5 years (range,58-92 years).The treatment methods included total hip arthroplasty in five patients and the bipolar hemiarthroplasty in 13.Harris score was used to evaluate the function outcome during the follow-up.Results The mean operation time lasted for 115 minutes,with intraoperative blood loss of 500 ml.Two patients presented with intraoperative complications.Of all the patients,three patients died within three months after operation and three patients were lost to follow-up.The remaining 12 patients received complete follow-up for a mean of 2.3 years ( range,1-7 years).One patient with hip joint dislocation was treated with closed reduction and distraction for three weeks.At the latest follow-up,the pain disappeared or was reduced in all the patients,including two patients with moderate pain and four with mild pain after some movements.The average Harris score was increased from preoperative 34 points to 83 points at one year after operation.Conclusions Endoprosthetic replacement is an effective salvage procedure for the failed treatment of the intertrochanteric fracture,as it can attain satisfactory pain relief and functional improvement.

0.05).Conclusion DTI can noninvasive detect the potential disorder of corpus eallosum in vivo,thus providing useful information to differentiate the cerebral ischemia disease from multiple sclerosis.

Actins ; metabolism ; Angiomyolipoma ; pathology ; Carcinoma, Renal Cell ; pathology ; Diagnosis, Differential ; Epithelioid Cells ; metabolism ; pathology ; Female ; Gastrointestinal Neoplasms ; pathology ; Gastrointestinal Stromal Tumors ; pathology ; Humans ; Kidney Neoplasms ; metabolism ; pathology ; Lung Neoplasms ; pathology ; Lymphangioleiomyomatosis ; pathology ; Male ; Melanoma ; pathology ; Melanoma-Specific Antigens ; metabolism ; Pancreatic Neoplasms ; pathology ; Perivascular Epithelioid Cell Neoplasms ; metabolism ; pathology ; Sarcoma, Clear Cell ; pathology ; Skin Neoplasms ; pathology ; Uterine Neoplasms ; pathology