Chromosome Aberrations ; Gene Fusion ; Genes, Tumor Suppressor ; Humans ; Oncogene Proteins, Fusion ; metabolism ; Oncogenes ; Pathology ; standards ; trends ; Soft Tissue Neoplasms ; genetics ; metabolism ; pathology

Actins ; metabolism ; Dermatofibrosarcoma ; classification ; pathology ; Desmin ; metabolism ; Head and Neck Neoplasms ; metabolism ; pathology ; Histiocytoma, Benign Fibrous ; classification ; metabolism ; pathology ; Histiocytoma, Malignant Fibrous ; classification ; metabolism ; pathology ; Humans ; Oncogene Proteins, Fusion ; metabolism ; Skin Neoplasms ; metabolism ; pathology ; Vimentin ; metabolism ; Xanthomatosis ; pathology

Biliary-pancreatic-duodenal junction is a special region in the human body.It is difficult to diagnose and treat diseases in this region because of the complicated anatomy,the limitation of diagnosing methods,the variety of disease,the high risks of operation and the severity of complications. We should pay more attention to the pathophysiology aspects and the laboratory researches of diseases in this region.Proper diagnosis and combination of different diagnosing methods could improve the rate of early diagnosis and identify the characteristics of the disease. Individualized operation should be considered to deal with iatrogenic injury of biliary-pancreatic-duodenal junction according to different patients' condition.The principle of operation is to control damage firstly and to preserve the function of biliary-pancreatic-duodenal junction and the physiological passway of bile and pancreatic juice to the greatest extent.

Objective To evaluate the safety and effect of adefovir dipivoxil (ADV) on hepatitis B in HBeAgpositive patients.Methods 131 patients were divided into two groups randomly.Two groups were treated with ADV (10mg/d)and matrine (0.6g/d),respectively.The rate of HBV negative,the normalization rates of ALT,HBeAg loss and anfi-HBe seroconversion were evaluated respectively.Results After 12 weeks of treatment,the rate of HBV negative,the normalization rates of ALT,HBeAg loss and anti-HBe seroconversion of ADV were all significantly higher than those of matrine group(53.2％ vs 7.8％,36.0％ vs 8.7％,13.6％ vs 3.1％,6.2％ vs 0,respectively,x2 =56.32,23.18,all P ＜ 0.01).Conclusion The domestic ADV superior to matrine in HBeAg-positive patients.

ObjectiveTo evaluate the clinical efficacy and toxicity of the regimen of paclitaxel combined with gemcitabine for anthracycine-resistant metastatic breast cancer. MethodsThirty patients with anthracycine-resistant metastatic breast cancer were enrolled in the study: paclitaxel 135mg/m2 infusion on day 1; Gemcitabine 850mg/m2 infusion on day 1,8 ,and administered in three weeks cycle. The clinical efficacy was evaluated every two cycles. ResultsOf all thirty patients qualified for efficacy analysis and among them CR 3 cases( 10％ ) ,PR 11 cases (36.7 ％ ), SD 11 cases ( 36.7％ ), and PD 5 cases ( 16.7％ ), with an overall response rate of 46.7％. The median TTP and overall survival in those 30 patients was 7.1 and 15.6 months. The main toxicities with grade 3 and 4 were hypoleucocytosis in 10 patients(30.0％ ) ,thrombocytopenia in 4 patients( 13.3％ ). ConclusionThe regimen of paclitaxel combined with gemcitabine was effective,well-tolerated scheme in the anthracycine-resistant metastatic breast cancer.

This study was aimed to further explore theory of small intestinal diseases and its principle of syndrome differentiation and treatment and lay the base for the clinical study, through the clinical research on the treatment of small intestinal diseases. Through the clinical survey among 584 patients, using Traditional Chinese Medicine (TCM) Clinical Multidimensional Relational Query System, which was established by the research on the clinical individual diagnosis and treatment evaluation system on the prevention and treatment of diseases, to conduct the multidimen-sional relational analysis on small intestinal diseases' disease-symptoms-syndromes-therapy-medicines-effects. Using data mining and statistical methods, such as WEKA, Business Objects, SPSS to mine and analyze the syn-dromes and symptoms. The results showed that commonly-used TCM methods on treatment of small intestinal dis-eases, therapeutic principles of main syndromes of small intestinal diseases were summarized. It was concluded that through scale-free network diagram analysis on the commonly-used treatment of small intestinal diseases, the main treatment methods included promotion the flow of qi, relieving pain, tonifying spleen, removing dampness, clearing away heat, mildly regulating cold and heat. And the treatment methods of dispersing cold, invigorating blood circula-tion and removing blood stasis, nourishing yin, moistening and loosening bowels, abductively dispersing food stagna-tion were also used. It corresponds with the results of clinical research.

Objective To set a RFID(Radio Frequency Identification) system for Gold Immunochromatographic Assay by using RFID-reading module,RFID tags and the master-MCU.Methods Radio frequency technology is characterized by the automatic identification and large dada-storage capacity.Electronic tags were packed with Gold Immunochromatographic Assay(GICA) strips and such data were stored in electronic tags as strip information,the test results,the identity of the tested,the test date,etc.The information were transmitted to PC through the RS-232 serial port,thus realizing the rapid and accurate recording and management of data.Results The application of RFID guaranteed the proper use of Gold Immunochromatographic Assay strips and improved the efficiency of sample management and data storage.Conclusion The application of RFID to Gold Immunochromatographic Assay can be used in general investigation of epidemic situation,biomedical detection and other relevant fields.

Objective To study the quality control method of Shaolin Zhenggujing tincture. Methods Angelicae Pubescentis Radix and Angelicae Sinensis Radix in the preparation were identified by TLC. The content of dracorhodin, the effective component of Resina Draconis, was determined by HPLC with acetonitrile-0.05 mol/L sodium dihydrogen phosphate (50∶50) as a mobile phase and 440 nm as the detection wavelength. Results Characteristic spots of TLC were clear with strong specificity and the negative test showed no interference. There was a good linear relationship for dracorhodin in the range of 3.997-43.97 ng and the recovery was 101.26% (RSD=2.84%). Concludion The qualitative and quantitative methods are accurate, reliable and repeatable, it can effectively control the inner quality of Shaolin Zhenggujing tincture.

Objective:To Construct a prokaryotic expression vector of cystatin C(Cys C)to provide a basis of purify Cys C protein produced by the expression system.Methods:Total RNA was isolated from HL-60 cells,and human Cys C gene was amplified with RT-PCR.The cDNA fragment was cloned into pET32a(+)vector,which was confirmed with sequencing and electrophoresis.Results:The result of electrophoresis showed that Cys C had been cloned into pET32a(+)vector,and the result of.DNA sequence analysis also showed the cloned Cys C gene sequence was completely correspondent to GeneBank data.Conclusion:The prokaryotic expression vector of cystatin C(Cys C)was obtained,providing a basis of purification of Cys C protein produced by the expression system.

Objective To study the adhesion and proliferation of rabbit bone marrow mesenchymal stem cells(BMSCs) on the HA/TCP bone graft scaffold.Methods BMSCs were harvested from the iliac bone and obtained by using adhesive culture method.BMSCs were induced with osteogenic medium,at the 7th day,the cells were stained by the calcium cobalt method to show the activities of alkaline phosphatase(ALP).At the 10th day,the mineralized nodules of osteoblasts stained with chinalizarin.Otherwise BMSCs were induced with adipogenic medium,and at the 21st day the cells stained with oil red O.We cultured the BMSCs on the surface of a matrix scaffold with osteogenic medium.The morphologic characters were checked by light microscopy,fluorescence microscopy and scanning electronic microscopy,and the proliferation of BMSCs were assayed using the MTT test.Results At the 7th day after osteoblastic induction,ALP was strongly positive,and at the 10th day,mineralized nodules stained with chinalizarin were jacinth.At the 21th day after adipogenic induction,the adipocytes were stained with oil red O.BMSCs grew well around or in pores of the HA/TCP bone graft scaffold and could be seen using both fluorescence microscopy and scanning electron microscopy.The MTT test assay showed that the HA/TCP couldn't inhibit the proliferation of BMSCs.Conclusion BMSCs have a good biocompatibility with a HA/TCP bone graft scaffold.