Chromosome Aberrations ; Gene Fusion ; Genes, Tumor Suppressor ; Humans ; Oncogene Proteins, Fusion ; metabolism ; Oncogenes ; Pathology ; standards ; trends ; Soft Tissue Neoplasms ; genetics ; metabolism ; pathology

Actins ; metabolism ; Dermatofibrosarcoma ; classification ; pathology ; Desmin ; metabolism ; Head and Neck Neoplasms ; metabolism ; pathology ; Histiocytoma, Benign Fibrous ; classification ; metabolism ; pathology ; Histiocytoma, Malignant Fibrous ; classification ; metabolism ; pathology ; Humans ; Oncogene Proteins, Fusion ; metabolism ; Skin Neoplasms ; metabolism ; pathology ; Vimentin ; metabolism ; Xanthomatosis ; pathology

Fibrin related markers include several proteins and fragments developed in the conversion of fibrinogen to cross-linked fibrin and following degradation.FDP played as a more important role than conventional global coagulation test before application of D-dimer in clinical settings,moreover,combination of FDP and D-dimer improve the diagnostic of DIC greatly,and D-dimer has become an effective tool excluding venous thromboembolism besides the imaging.Whether basic or clinical research on soluble fibrin monomers and complexes were focused in an early stage,however,limited by diversity of analytes and nonstandardization,no full application was come into being.It is worthwhile to note that fibrin related markers release into plasma sequentially from initialization of fibrinogen activation to fibrinolysis.Thus,the combination of these index is useful to understand patient's condition (such as DIC),prognosis of VTE recurrence,and prophylaxes of post-operation thrombosis.

Objective To explore the effect of fluticasone propionate nasal spray(Fu Shu-liang) in improving children's symptoms of allergic rhinitis and recurrent asthma.Methods 100 children with allergic rhinitis and asthma syndrome were randomly divided into control group and experimental group.The control group was treated with loratadine.The experimental group was treated with water-soluble auxiliary Fu Shu-liang nasal spay on the basis of the control group.The treatment effects were observed.Results The total effective rate of the observation group was 94％,which was significantly higher than 76％ of the control group (x2 =6.35,P ＜ 0.05).After 10-12 weeks,the rhinitis symptom score and asthma symptom score between the two groups had statistically significant differences (t =2.47,2.64,3.41 ; 2.30,3.17,2.47,all P ＜ 0.05).The incidence rate of adverse reactions such as nasal dryness,epistaxis between the two groups had statistically significant differences (x2 =7.11,7.53,all P ＜ 0.05).Conclusion Fu Shu-liang(Eph propionate and fluticasone propionate nasal spray) has good effect in improving symptoms of children with allergic rhinitis and asthma,it is worthy of clinical vigorously promoted.

ObjectiveTo evaluate the clinical efficacy and toxicity of the regimen of paclitaxel combined with gemcitabine for anthracycine-resistant metastatic breast cancer. MethodsThirty patients with anthracycine-resistant metastatic breast cancer were enrolled in the study: paclitaxel 135mg/m2 infusion on day 1; Gemcitabine 850mg/m2 infusion on day 1,8 ,and administered in three weeks cycle. The clinical efficacy was evaluated every two cycles. ResultsOf all thirty patients qualified for efficacy analysis and among them CR 3 cases( 10％ ) ,PR 11 cases (36.7 ％ ), SD 11 cases ( 36.7％ ), and PD 5 cases ( 16.7％ ), with an overall response rate of 46.7％. The median TTP and overall survival in those 30 patients was 7.1 and 15.6 months. The main toxicities with grade 3 and 4 were hypoleucocytosis in 10 patients(30.0％ ) ,thrombocytopenia in 4 patients( 13.3％ ). ConclusionThe regimen of paclitaxel combined with gemcitabine was effective,well-tolerated scheme in the anthracycine-resistant metastatic breast cancer.

The new current versions of Hospital Finance System and Hospital Accounting System promote the reform of medicine accounting. It requires the method of the drugs cost accounting to change the retailing price accounting to the purchasing price accounting. It clearly defines individual valuation method, “first-in, first-out” method or weighted average method should be used to determine the actual cost of the materials based on the actual situation. It analyzes the problems of medical management and cost accounting, and proposes the practical solutions to strengthen the standard management of medicine and the medical cost accounting.

Objective To investigate the causes and preventive measures of subclavian venous catheter-related infections oc-curred in traumatic hemorrhagic shock patients .Methods 336 traumatic hemorrhagic shock patients indwelling subclavian vein catheter was included to explore the causes and treatment of catheter-related infections .The outcome was infect rate and strains dis-trubtion .Results Of 336 patients with subclavian vein catheterization average time was 4 .3 days ,including 27 cases of infection , and the infection rate was 8 .04% .All patients were discharged after active treatment ,with no deaths .Conclusion Traumatic hem-orrhagic shock patients with subclavian vein catheter are safe and effective .Risk factors for infection ,improve treatment and patient care ,shorten the time of catheter ,can effectively prevent catheter infections .

Molecular targeted agents changed the therapeutic pattern on gastrointestinal stromal tumor (GIST).Imatinib of 400 mg/d is recommended to be the first line therapy for metastatic GIST.Escalation of imatinib dose or sunitinib could improve the overall survival of patients when the treatment with standard dose of imatinib failed.In addition,new targeted agents showed potential anti-tumor efficacy.Imatinib adjuvant therapy could improve the recurrence-free survival of GIST patients with median or high risk after complete tumor resection.Preoperative treatment with imatinib raises tumor resection rate,but whether it could provide survival benefit has not been proved.C-kit/PDGFRα gene mutation can predict the efficacy of imatinib and sunitinib,and it is helpful to screen out the GIST patients to receive imatinib adjuvant therapy.

Objective To evaluate the safety and effect of adefovir dipivoxil (ADV) on hepatitis B in HBeAgpositive patients.Methods 131 patients were divided into two groups randomly.Two groups were treated with ADV (10mg/d)and matrine (0.6g/d),respectively.The rate of HBV negative,the normalization rates of ALT,HBeAg loss and anfi-HBe seroconversion were evaluated respectively.Results After 12 weeks of treatment,the rate of HBV negative,the normalization rates of ALT,HBeAg loss and anti-HBe seroconversion of ADV were all significantly higher than those of matrine group(53.2％ vs 7.8％,36.0％ vs 8.7％,13.6％ vs 3.1％,6.2％ vs 0,respectively,x2 =56.32,23.18,all P ＜ 0.01).Conclusion The domestic ADV superior to matrine in HBeAg-positive patients.

BACKGROUND: Cathepsin E, an intracellular asparUc proteinase of pepsin family, Is composed of 2 homologous domains, each containing a consensus DTG motif, in which the role of aspartic acid (Asp98) is still unknown. OBJECTIVE: To determine the role of Asp98 in the catalysis of Cathepsin E. DESIGN, TIME AND SETTING: An observation experiment was performed in the Guangdong Pharmacological College between November 2007 and December 2008.MATERIALS: Rat Cathepsin E antibody was prepared according to the references, and the purified with affinity chromatography. PcDNA3.0 was offered from Guangzhou Ganewindows Biotech Ltd. Human embryo kidney 293 cell strain was offered from Stem Cell Bank,Chinese Academy of Sciences.METHODS: The cDNA containing full-length rat Cathepsln E was inserted into pBluescdpt IISK plasmid between Smal and Xhol. Asp98 was replaced with an acidic amino acid termed glutamic acid to produce a D98E mutant by Kunkel gene site-directad mutagenesis and expressed in human embryo renal cells.MAIN OUTCOME MEASURES: Expression of wild type and mutant protein was determined, the wild type and mutant Cathepsin E was purified, the enzyme activity, folding and self-precessing were analyzed. RESULTS: Hetaroganous expression of wild type and mutant rat Cathepsin E were found in human embryo kidney 293 cells, and both were expressed in the cell culture media and cell lysate. Mutant D98E was still able to auto-activate into mature enzyme, although its activity was only 13% of that of wild type. The Km value of mutant was similar to that of wild type, but its K<,cat> value was significantly smaller than that of wild type. Both mutant and wild type were inhibited by pepstatin and Ascaris pepsin inhibitor with similar Ki values. The CD spectra of mutant and wild type were similar.CONCLUSION: The Asp98 plays an important role in catalysis, and it can be partly replaced by glutamic acid. Mutant D98E has a lowered activity since the mutation affects the catalytic efficiency.